Evaluation of coffee ground as a feedstuff in practical diets for olive flounder Paralichthys olivaceus

Rahimnejad, S.,Lee, S.M. Academic Press 2016 FISH AND SHELLFISH IMMUNOLOGY Vol.53 No.-

Comparison of Free and Dipeptide Lysine Utilization in Diets for Juvenile Olive Flounder Paralichthys olivaceus

Rahimnejad, Samad,Lee, Kyeong-Jun The Korean Society of Fisheries and Aquatic Scienc 2014 Fisheries and Aquatic Sciences Vol.17 No.4

We compared the utilization efficiency of free lysine (FL) and dipeptide lysine-glycine (LG) in terms of growth performance and whole-body amino acid composition in olive flounder Paralichthys olivaceus. A basal experimental diet was formulated to contain 0.5% (basal) lysine from fish meal, and four other diets were prepared by supplementing 0.5% or 1.0% of either FL or LG. The experiment was performed in triplicate, and 20 randomly selected fish averaging $5.41{\pm}0.16g$ were fed one of the test diets at the rate of 3% BW/day twice daily for 6 weeks. At the end of the feeding trial, fish fed the basal diet showed significantly less weight gain than did the other groups. The results of a two-way ANOVA showed that both lysine level (P = 0.001) and type (P = 0.034) influenced growth rate; however, we found no significant interaction between lysine level and form (P > 0.05). Our results revealed a significant improvement in protein efficiency ratio (PER) with each increment of dietary lysine, and the groups fed LG-supplemented diets showed higher PER than did those offered FL. Hepatosomatic and viscerosomatic indices were significantly influenced by lysine level and form, and higher values were recorded in fish fed diets containing LG. Significantly higher whole-body arginine levels were found in LG-fed groups, and a significant interaction was observed between lysine level and form (P = 0.009). Whole-body valine and aspartic acid contents were affected by lysine level, and alanine concentration was influenced by both lysine level and form. Our findings indicate that juvenile olive flounder can utilize LG more efficiently than FL for protein synthesis.

Evaluation of Coffee Ground as a Feedstuff in Practical Diets for Olive Flounder Paralichthys olivaceus

Rahimnejad, Samad,Choi, Jin,Lee, Sang-Min The Korean Society of Fisheries and Aquatic Scienc 2015 Fisheries and Aquatic Sciences Vol.18 No.3

A 10-week feeding trial was carried out to investigate the feasibility of using spent coffee ground (CG) as a potential feed ingredient for olive flounder. Growth, feed utilization, body composition and antioxidant enzyme activity were examined. A control diet was formulated and three other diets were prepared to contain 5, 10 or 15% CG (designated as Con, CG5, CG10 and CG15, respectively) by replacing for wheat flour. Two hundred forty fish ($104{\pm}0.7g/fish$) were allotted to 12 circular tanks of 400 L capacity at a density of 20 fish per tank and fed the experimental diets twice daily. At the end of the feeding trial, fish fed the CG5 diet exhibited significantly (P < 0.05) higher growth performance than those fed the control diet. Also, fish fed the CG10 diet had a comparable growth to that of the control group, but further increase of dietary CG inclusion level to 15% resulted in significant decrease of growth performance. Fish fed the CG15 diet showed significantly lower feed efficiency and protein efficiency ratio than other treatments. Significantly lower muscle protein content was observed in fish fed CG15 diet compared to the control. Significant reduction in plasma cholesterol concentration was found in fish fed CG15 diet compared to control. No significant changes were found in alkyle and superoxide radicals scavenging activities of plasma, muscle and liver among dietary treatments. Also, liver total protein, total antioxidant capacity, catalase and glutathione peroxidase activities were not significantly influenced by dietary inclusion of CG. According to these results, we concluded that CG can be included up to 10% in the diet for olive flounder without any adverse effects on growth, feed utilization and antioxidant enzyme activity.

Comparison of Free and Dipeptide Lysine Utilization in Diets for Juvenile Olive Flounder Paralichthys olivaceus

Samad Rahimnejad,이경준 한국수산과학회 2014 Fisheries and Aquatic Sciences Vol.17 No.4

We compared the utilization efficiency of free lysine (FL) and dipeptide lysine–glycine (LG) in terms of growth performance and whole-body amino acid composition in olive flounder Paralichthys olivaceus. A basal experimental diet was formulated to contain 0.5% (basal) lysine from fish meal, and four other diets were prepared by supplementing 0.5% or 1.0% of either FL or LG. The experiment was performed in triplicate, and 20 randomly selected fish averaging 5.41 ± 0.16 g were fed one of the test diets at the rate of 3% BW/day twice daily for 6 weeks. At the end of the feeding trial, fish fed the basal diet showed significantly less weight gain than did the other groups. The results of a two-way ANOVA showed that both lysine level (P = 0.001) and type (P = 0.034) influenced growth rate; however, we found no significant interaction between lysine level and form (P > 0.05). Our results revealed a significant improvement in protein efficiency ratio (PER) with each increment of dietary lysine, and the groups fed LG-supplemented diets showed higher PER than did those offered FL. Hepatosomatic and viscerosomatic indices were significantly influenced by lysine level and form, and higher values were recorded in fish fed diets containing LG. Significantly higher whole-body arginine levels were found in LG-fed groups, and a significant interaction was observed between lysine level and form (P = 0.009). Whole-body valine and aspartic acid contents were affected by lysine level, and alanine concentration was influenced by both lysine level and form. Our findings indicate that juvenile olive flounder can utilize LG more efficiently than FL for protein synthesis.

Improvement of sediment microbial fuel cell performance by application of sun light and biocathode

Mostafa Rahimnejad,Zahra Najafgholi 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.1

Three series of experiments were conducted to improve sediment microbial fuel cell (SMFC) performance. At first, dissolved oxygen level of catholyte was increased with native seaweed of the Caspian Sea. Power output was improved about 2-fold, and maximum power density of 46.148mW/m2 was produced in the presence of seaweed as biocathode in cathode compartment. Secondly, the best depth to embed anode was then determined. Anode was embedded in 3, 6, 9 and 12 cm below the sediment/water interface. The best depth to bury the anode was finally determined in 3 cm below the sediment/water interface, maximum generated power and current density of 42.156mW/m2 and 282.92 mA/m2, were respectively obtained in this depth. In addition, influence of agitated flow on power generation from SMFC was investigated.

Thionine Increases Electricity Generation from Microbial Fuel Cell Using Saccharomyces cerevisiae and Exoelectrogenic Mixed Culture

Mostafa Rahimnejad,Ghasem Darzi Najafpour,Ali Asghar Ghoreyshi,Farid Talebnia,Giuliano C. Premier,Gholamreza Bakeri,김중래,오상은 한국미생물학회 2012 The journal of microbiology Vol.50 No.4

Microbial fuel cells (MFCs) have been shown to be capable of clean energy production through the oxidation of biodegradable organic waste using various bacterial species as biocatalysts. In this study we found Saccharomyces cerevisiae,previously known electrochemcially inactive or less active species, can be acclimated with an electron mediator thionine for electrogenic biofilm formation in MFC, and electricity production is improved with facilitation of electron transfer. Power generation of MFC was also significantly increased by thionine with both aerated and non-aerated cathode. With electrochemically active biofilm enriched with swine wastewater, MFC power increased more significantly by addition of thionine. The optimum mediator concentration was 500 mM of thionine with S. cerevisae in MFC with the maximum voltage and current generation in the microbial fuel cell were 420 mV and 700 mA/m2, respectively. Cyclic voltametry shows that thionine improves oxidizing and reducing capability in both pure culture and acclimated biofilm as compared to non-mediated cell. The results obtained indicated that thionine has great potential to enhance power generation from unmediated yeast or electrochemically active biofilm in MFC.

Optimum dietary protein to energy ratio for red seabream (Pagrusmajor)

Samad Rahimnejad,Dae-Han Oh,Sung-Sam Kim,Se-Min Choi,Kyeong-Jun Lee 한국수산과학회 양식분과 2012 한국수산과학회 양식분과 학술대회 Vol.2012 No.6

Evaluation of Coffee Ground as a Feedstuff in Practical Diets for Olive Flounder Paralichthys olivaceus

Samad Rahimnejad,최진,이상민 한국수산과학회 2015 Fisheries and Aquatic Sciences Vol.18 No.3

A 10-week feeding trial was carried out to investigate the feasibility of using spent coffee ground (CG) as a potential feed ingredient for olive flounder. Growth, feed utilization, body composition and antioxidant enzyme activity were examined. A control diet was formulated and three other diets were prepared to contain 5, 10 or 15% CG (designated as Con, CG5, CG10 and CG15, respectively) by replacing for wheat flour. Two hundred forty fish (104 ± 0.7 g/fish) were allotted to 12 circular tanks of 400 L capacity at a density of 20 fish per tank and fed the experimental diets twice daily. At the end of the feeding trial, fish fed the CG5 diet exhibited significantly (P < 0.05) higher growth performance than those fed the control diet. Also, fish fed the CG10 diet had a comparable growth to that of the control group, but further increase of dietary CG inclusion level to 15% resulted in significant decrease of growth performance. Fish fed the CG15 diet showed significantly lower feed efficiency and protein efficiency ratio than other treatments. Significantly lower muscle protein content was observed in fish fed CG15 diet compared to the control. Significant reduction in plasma cholesterol concentration was found in fish fed CG15 diet compared to control. No significant changes were found in alkyle and superoxide radicals scavenging activities of plasma, muscle and liver among dietary treatments. Also, liver total protein, total antioxidant capacity, catalase and glutathione peroxidase activities were not significantly influenced by dietary inclusion of CG. According to these results, we concluded that CG can be included up to 10% in the diet for olive flounder without any adverse effects on growth, feed utilization and antioxidant enzyme activity.

EVALUATION OF SUPPLEMENTAL EFFECTS OF Pediococcus acidilactici AND GALACTOOLOGOSACCHARIDE SINGLY OR COMBINED IN DIETS FOR ROCKFISH Sebastes schlegeli

Samad Rahimnejad,Seyed Hossein Hoseinifar,M. Angeles Esteban,Mathieu Castex,Sang-Min Lee 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

Effects of Dietary Supplementation of Spirulina and Quercetin on Growth, Innate Immune Responses, Disease Resistance Against Edwardsiella tarda, and Dietary Antioxidant Capacity in the Juvenile Olive Flounder Paralichthys olivaceus

김성삼,Samad Rahimnejad,김강웅,이봉주,이경준 한국수산과학회 2013 Fisheries and Aquatic Sciences Vol.16 No.1

A 10-week feeding trial was conducted to examine the effects of dietary spirulina and quercetin on growth, innate immunity, disease resistance and dietary antioxidant capacity in the juvenile olive flounder. Triplicate groups of fish (initial body weight, 2.9 ± 0.01 g) were fed one of isonitrogenous (48% crude protein) and isocaloric (17.4 MJ/kg DM) experimental diets containing 0% spirulina (as a control), 3.4% spirulina, or 6.8% spirulina with or without supplementation of 0.5% quercetin (designated as CON, SP3.4, SP6.8, and SP6.8 + Q, respectively) at a rate of 3% body mass twice daily. Higher dietary antioxidant capacity was found with spirulina supplementation, and the highest value (P < 0.05) was obtained with SP6.8 + Q diet. At the end of the feeding trial, no significant effects were observed on growth performance, body composition and disease resistance against Edwardsiella tarda. Lysozyme activity was significantly increased by spirulina supplementation (P < 0.05), and the highest value was observed in the group fed SP6.8 + Q diet. Also, significantly higher respiratory burst activity (P < 0.05) was found in SP3.4 group. According to the results of this study, dietary supplementation of 3.4% spirulina may enhance innate immunity of olive flounder.