Effect of Trichostatin A on Anti HepG2 Liver Carcinoma Cells: Inhibition of HDAC Activity and Activation of Wnt/β-Catenin Signaling

Shi, Qing-Qiang,Zuo, Guo-Wei,Feng, Zi-Qiang,Zhao, Lv-Cui,Luo, Lian,You, Zhi-Mei,Li, Dang-Yang,Xia, Jing,Li, Jing,Chen, Di-Long Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Purpose: To investigate the effect of deacetylase inhibitory trichostatin A (TSA) on anti HepG2 liver carcinoma cells and explore the underlying mechanisms. Materials and Methods: HepG2 cells exposed to different concentrations of TSA for 24, 48, or 72h were examined for cell growth inhibition using CCK8, changes in cell cycle distribution with flow cytometry, cell apoptosis with annexin V-FTIC/PI double staining, and cell morphology changes under an inverted microscope. Expression of ${\beta}$-catenin, HDAC1, HDAC3, H3K9, CyclinD1 and Bax proteins was tested by Western blotting. Gene expression for ${\beta}$-catenin, HDAC1and HDAC3 was tested by q-PCR. ${\beta}$-catenin and H3K9 proteins were also tested by immunofluorescence. Activity of Renilla luciferase (pTCF/LEF-luc) was assessed using the Luciferase Reporter Assay system reagent. The activity of total HDACs was detected with a HDACs colorimetric kit. Results: Exposure to TSA caused significant dose-and time-dependent inhibition of HepG2 cell proliferation (p<0.05) and resulted in increased cell percentages in G0/G1 and G2/M phases and decrease in the S phase. The apoptotic index in the control group was $6.22{\pm}0.25%$, which increased to $7.17{\pm}0.20%$ and $18.1{\pm}0.42%$ in the treatment group. Exposure to 250 and 500nmol/L TSA also caused cell morphology changes with numerous floating cells. Expression of ${\beta}$-catenin, H3K9and Bax proteins was significantly increased, expression levels of CyclinD1, HDAC1, HDAC3 were decreased. Expression of ${\beta}$-catenin at the genetic level was significantly increased, with no significant difference in HDAC1and HDAC3 genes. In the cytoplasm, expression of ${\beta}$-catenin fluorescence protein was not obvious changed and in the nucleus, small amounts of green fluorescence were observed. H3K9 fluorescence protein were increased. Expression levels of the transcription factor TCF werealso increased in HepG2 cells following induction by TSA, whikle the activity of total HDACs was decreased. Conclusions: TSA inhibits HDAC activity, promotes histone acetylation, and activates Wnt/${\beta}$-catenin signaling to inhibit proliferation of HepG2 cell, arrest cell cycling and induce apoptosis.

An Interactive Planning and Scheduling Framework for Optimising Pits-to-Crushers Operations

Shi Qiang Liu,Erhan Kozan 대한산업공학회 2012 Industrial Engineeering & Management Systems Vol.11 No.1

In this paper, an interactive planning and scheduling framework are proposed for optimising operations from pits to crushers in ore mining industry. Series of theoretical and practical operations research techniques are investigated to improve the overall efficiency of mining systems due to the facts that mining managers need to tackle optimisation problems within different horizons and with different levels of detail. Under this framework, mine design planning, mine production sequencing and mine transportation scheduling models are integrated and interacted within a whole optimisation system. The proposed integrated framework could be used by mining industry for reducing equipment costs, improving the production efficiency and maximising the net present value.

Ankrd7, a Novel Gene Specifically Expressed in Sertoli Cells and Its Potential Roles in Sertoli Cell Maturation

Yu-Qiang Shi,Lian-Cai Du,Qing-Zhong Wang,Chun-Fang Han 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.2

The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a maturation-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immunohistochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermatogenesis.

An Interactive Planning and Scheduling Framework for Optimising Pits-to-Crushers Operations

Liu, Shi Qiang,Kozan, Erhan Korean Institute of Industrial Engineers 2012 Industrial Engineeering & Management Systems Vol.11 No.1

In this paper, an interactive planning and scheduling framework are proposed for optimising operations from pits to crushers in ore mining industry. Series of theoretical and practical operations research techniques are investigated to improve the overall efficiency of mining systems due to the facts that mining managers need to tackle optimisation problems within different horizons and with different levels of detail. Under this framework, mine design planning, mine production sequencing and mine transportation scheduling models are integrated and interacted within a whole optimisation system. The proposed integrated framework could be used by mining industry for reducing equipment costs, improving the production efficiency and maximising the net present value.

Ileus tube placement combined with laparoscopy in the treatment of refractory small bowel obstruction: Case series and literature review

Wei-Hai Shi,Hao-Qiang Chao,Nian-Yuan Ye,Hong-Jian Shi 소화기인터벤션의학회 2019 Gastrointestinal Intervention Vol.8 No.1

Post-surgery small bowel obstruction (SBO) has substantial morbidity and mortality. Even with refinement of surgical technique, SBO still remains challenging with the most common causes of acute hospital admission. Most SBOs can be managed with conservative treatment. However, the pal-liative methods may fail to relieve the symptoms in some clinical settings. Currently, laparotomy is the standard of care for difficult SBO. And lapa-roscopy offers an alternative approach for operative intervention in SBO and was first described by Bastug et al in 1991. Emerging literature dem-onstrated that laparoscopic surgery for SBO is less invasive, resulting in a shorter hospital stay than open surgery. And most recurrent adhesive SBO can be managed with ileus tube placement. This case series is to evaluate the efficacy of transnasal ileus tube placement combined with laparoscopic surgery in the treatment of consecutive 5 cases of refractory SBO post oncologic gastrointestinal surgery.

Ileus tube placement combined with laparoscopy in the treatment of refractory small bowel obstruction: Case series and literature review

Wei-Hai Shi,Hao-Qiang Chao,Nian-Yuan Ye,Hong-Jian Shi 소화기인터벤션의학회 2019 International journal of gastrointestinal interven Vol.8 No.1

Post-surgery small bowel obstruction (SBO) has substantial morbidity and mortality. Even with refinement of surgical technique, SBO still remains challenging with the most common causes of acute hospital admission. Most SBOs can be managed with conservative treatment. However, the pal-liative methods may fail to relieve the symptoms in some clinical settings. Currently, laparotomy is the standard of care for difficult SBO. And lapa-roscopy offers an alternative approach for operative intervention in SBO and was first described by Bastug et al in 1991. Emerging literature dem-onstrated that laparoscopic surgery for SBO is less invasive, resulting in a shorter hospital stay than open surgery. And most recurrent adhesive SBO can be managed with ileus tube placement. This case series is to evaluate the efficacy of transnasal ileus tube placement combined with laparoscopic surgery in the treatment of consecutive 5 cases of refractory SBO post oncologic gastrointestinal surgery.

Serum Peroxiredoxin3 is a Useful Biomarker for Early Diagnosis and Assessemnt of Prognosis of Hepatocellular Carcinoma in Chinese Patients

Shi, Liang,Wu, Li-Li,Yang, Jian-Rong,Chen, Xiao-Fei,Zhang, Yi,Chen, Zeng-Qiang,Liu, Cun-Li,Chi, Sheng-Ying,Zheng, Jia-Ying,Huang, Hai-Xia,Yu, Fu-Jun,Lin, Xiang-Yang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: Recently, peroxiredoxin3 (PRDX3) was identified as a novel molecular marker for the progression of hepatocellular carcinoma (HCC). However, its potential clinical application as a serum marker for the early diagnosis and prognosis of HCC has not been investigated. Methods: PRDX3, alpha-fetaprotein (AFP), and other biochemical parameters were measured in serum samples from 297 Chinese patients, including 96 with HCC, 98 with liver cirrhosis (LC), and 103 healthy controls (HCs). Correlations between serum PRDX3 expression and clinicopathological variables and the relationship between serum PRDX3 expression and prognosis were analyzed. Results: Serum PRDX3 was significantly higher in HCC patients than in the LC and HC groups. The sensitivity and specificity of serum PRDX3 for the diagnosis of HCC were 85.9% and 75.3%, respectively, at a cutoff of 153.26 ng/mL, and the area under the curve was 0.865. Moreover, serum PRDX3 expression was strongly associated with AFP level, tumor diameter, TNM stage, and portal vein invasion. Kaplan-Meier curve analysis revealed that HCC patients with high serum PRDX3 expression had a shorter median survival time than those with low PRDX3 expression. Moreover, serum PRDX3 expression was an independent risk factor for overall survival. The inverse correlation between serum PRDX3 and patient survival remained significant in patients with early-stage HCC and in those with normal serum AFP levels. Conclusions: Serum PRDX3 can be used as a noninvasive biomarker for the diagnosis and/or prognosis of HCC.

Synergistic effect of adsorption and photocatalysis of BiOBr/lignin-biochar composites with oxygen vacancies under visible light irradiation

Qiang Yang,Xiang Li,Qingwen Tian,Aixiang Pan,Xingjian Liu,Hang Yin,Yingqiao Shi,Guigan Fang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

Effective utilization solar energy through photocatalysis is an ideal way to solve environmental problemsand achieve sustainable development. Herein, a novel BiOBr/Lignin-Biochar photocatalyst has been successfullysynthesized by a simple hydrothermal method. The number of oxygen vacancies of BiOBrincreased after C doping, which improves visible-light absorbance, reduces the recombination ofphoto-generated carriers and promotes O2 activation to produce O2. UV–vis DRS result demonstratedthat the visible-light absorption capacity of BiOBr improved significantly with the addition of lignin. Compared with BiOBr, the adsorption and photocatalytic ability of BiOBr/Lignin-Biochar composites weregreatly enhanced due to enriched oxygen vacancies and the congenerous effect between BiOBr andlignin-biochar. The RhB removal with pure BiOBr and BiOBr/Lignin-Biochar under visible-light irradiationat 60 min was 54.5% and 99.2%, respectively, owing to the interface interaction between BiOBr and ligninbiocharpromoted the separation between electron and holes and the enrichment of RhB around the photocatalysts. Notably, the bandgap of BiOBr/Lignin-Biochar composites decreased from 2.65 eV to 2.56 eVafter C doping, useful for visible-light-driven photocatalysis. The superoxide radical anions (O2) were themain active species, as demonstrated by free radical capture experiments and ESR characterizationresults. Hence, the present work provides new insights into constructing cost-effective, high-efficiencycomposite materials for environmental remediation.

Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells

Qiang Fu,Huijun Shi,Yan Ren,Fei Guo,Wei Ni,Jun Qiao,Pengyan Wang,Hui Zhang,Chuangfu Chen 한국미생물학회 2014 The journal of microbiology Vol.52 No.7

Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.

Analysis of restrained steel beams subjected to heating and cooling Part II: Validation and parametric studies

Shi-Xiong Guo,Guo-Qiang Li 국제구조공학회 2008 Steel and Composite Structures, An International J Vol.8 No.1

This paper presents the results of a validation and parametric study for the theory presented in the companion paper. The parameters investigated include the stiffness of axial and rotational restraints, load ratio, depth-span ratio of the beam, the yield strength of steel, load type and the temperature distribution in the crosssection of the beam.