From "View"(境象) to "Flavour"(韻味)

Liu, Ning 성균관대학교 동아시아학술원 2002 Sungkyun Journal of East Asian Studies Vol.2 No.1

From "View"(境象) to "Flavour"(韻味)

Genome-wide analyses of the NAC transcription factor family to reveal the potential candidate genes responding to powdery mildew in balsam pear

Ning Yu,Liu Jing,Song Bo,Xu Hai,Liu Zhiyang,Chen Longzheng 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.6

Powdery mildew (PM) is the most serious disease in balsam pear and usually causes severe yield and quality decreases. Although NAC transcription factors involved in the growth, development, and regulation of biotic and abiotic stress responses have been published for many crops, comprehensive data regarding the structure, evolution, and functions of the NAC family in balsam pear are still unavailable. In this study, a comprehensive analysis of the NAC transcription factor family in balsam pear was performed, and 90 NAC genes of balsam pear (McNAC) were identified and divided into 18 subfamilies. Gene structure and protein motif analyses revealed that the functions of McNAC proteins exhibit diversity during evolution and are mainly involved in the response to light, growth, development, and abiotic and biotic stresses. Fifteen McNAC genes (McNAC03, McNAC09, McNAC15, McNAC19, McNAC25, McNAC29, McNAC34, McNAC47, McNAC55, McNAC59, McNAC61, McNAC69, McNAC71, McNAC73, and McNAC78) were differentially expressed after PM pathogen infection. Moreover, the predicted interacting proteins of 7 genes (McNAC09, McNAC15, McNAC25, McNAC29, McNAC34, McNAC73, and McNAC78) were related to the plant hormone signal transduction, betalain biosynthesis, and sphingolipid metabolism pathways by transcriptomic data, implying that the 7 genes participated in PM pathogen infection through these pathways. Metabolome data showed that nine metabolites in the same pathways were changed after PM infection, which suggests that these 7 McNAC genes could regulate these metabolites through the pathways mentioned above to respond to PM infection. The expression levels of McNAC09, McNAC15, McNAC25 and McNAC34 were further confirmed by qPCR to show the reliability of the obtained RNA-seq data. Interestingly, McNAC34 was predicted to be targeted by miRNA164, indicating that McNAC34 might be involved in the response to PM infection by the miRNA-regulated pathway. This study will provide deep insight into NAC gene family evolution and functions, especially in the response to PM pathogen infection in balsam pear.

p38 MAPK Signaling Mediates Mitochondrial Apoptosis in Cancer Cells Induced by Oleanolic Acid

Liu, Jia,Wu, Ning,Ma, Lei-Na,Zhong, Jia-Teng,Liu, Ge,Zheng, Lan-Hong,Lin, Xiu-Kun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Oleanolic acid (OA) is a nutritional component widely distributed in various vegetables. Although it has been well recognized for decades that OA exerts certain anti-tumor activity by inducing mitochondria-dependent apoptosis, it is still unclear that what molecular signaling is responsible for this effect. In this study, we employed cancer cell lines, A549, BXPC-3, PANC-1 and U2OS to elucidate the molecular mechanisms underlying OA anti-tumor activity. We found that activation of MAPK pathways, including p-38 MAPK, JNK and ERK, was triggered by OA in both a dose and time-dependent fashion in all the tested cancer cells. Activation was accompanied by cleavage of caspases and PARP as well as cytochrome C release. SB203580 (p38 MAPK inhibitor), but not SP600125 (JNK inhibitor) and U0126 (ERK inhibitor), rescued the pro-apoptotic effect of OA on A549 and BXPC-3 cells. OA induced p38 MAPK activation promoted mitochondrial translocation of Bax and Bim, and inhibited Bcl-2 function by enhancing their phosphorylation. OA can induce reactive oxygen species (ROS)-dependent ASK1 activation, and this event was indispensable for p38 MAPK-dependent apoptosis in cancer cells. In vivo, p38 MAPK knockdown A549 tumors proved resistant to the growth-inhibitory effect of OA. Collectively, we elucidated that activation of ROS/ASK1/p38 MAPK pathways is responsible for the apoptosis stimulated by OA in cancer cells. Our finding can contribute to a better understanding of molecular mechanisms underlying the antitumor activity of nutritional components.

haracterization of a Recombinant Thermostable Xylanase from Hot Spring Thermophilic Geobacillus sp. TC-W7

( Liu Bin ),( Ning Ning Zhang ),( Chao Zhao ),( Bai Xue Lin ),( Lian Hui Xie ),( Yi Fan Huang ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.10

A xylanase-producing thermophilic strain, Geobacillus sp. TC-W7, was isolated from a hot spring in Yongtai (Fuzhou, China). Subsequently, the xylanase gene that encoded 407 amino acids was cloned and expressed. The recombinant xylanase was purified by GST affinity chromatography and exhibited maximum activity at 75℃ and a pH of 8.2. The enzyme was active up to 95℃ and showed activity over a wide pH range of 5.2 to 10.2. Additionally, the recombinant xylanase showed high thermostability and pH stability. More than 85% of the enzyme`s activity was retained after incubation at 70℃ for 90 min at a pH of 8.2. The activity of the recombinant xylanase was enhanced by treatment with 10 mM enzyme inhibitors (DDT, Tween-20, 2-Me, or TritonX-100) and was inhibited by EDTA or PMSF. Its functionality was stable in the presence of Li+, Na+, and K+, but inhibited by Hg2+, Ni2+, Co2+, Cu2+, Zn2+, Pb2+, Fe3+, and Al3+. The functionality of the crude xylanase had similar properties to the recombinant xylanase except for when it was treated with Al2+ or Fe2+. The enzyme might be a promising candidate for various industrial applications such as the biofuel, food, and paper and pulp industries.

Similar Singer Search by Property of Vocal Sound through Weighted Euclidean Distance

Ning-Han Liu 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.6

Research on Dust Migration Law and Dust Reduction Efficiency in the Operation Process of Tunnel Boring Machine

Ning Liu,Kun Chen,Piao Jiang,Yi-Xiong Huang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.3

At present, most dust reduction methods do not consider the difference of dust particle diameter distribution along the tunnel and the applicability of droplet group under different parameters. In order to improve the dust reduction efficiency of each section, the particle size analyzer was used to analyze the dust samples of each section along the tunnel, and the migration law of dust of each particle diameter during the construction of Tunnel Boring Machine (TBM) was studied. The dust falling efficiency of droplets with different particle diameter under different sets of parameters was determined by the experimental platform built by ourselves. Research indicates: along the axis of tunnel, the farther away from the face of the tunnel, the higher the peak volume frequency of dust, and the distribution range of dust gradually narrowed to the direction of small particle diameter. The settling distance of dust with different particle diameter is quite different, and the content of small particle diameter dust shows an upward trend as a whole. The reduction efficiency of dust first increases and then decreases with the particle diameter, and there is particle diameter of the optimum dust reduction efficiency under the action of the same droplet group. The calculation formula of the air and water flow of the air-water nozzle and the particle diameter of the optimum dust reduction efficiency are put forward. This research can provide basis and guidance for multi-stage dust reduction and nozzle parameter setting in different sections of the tunnel.

Improving Energy Efficiency in Discrete Parts Manufacturing System using an Ultra-Flexible Job Shop Scheduling Algorithm

Ning Liu,Y. F. Zhang,Wen Feng Lu 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.6 No.2

Improving energy efficiency has been one of main objectives in modern manufacturing enterprises. Various approaches aiming at efficient energy management have been proposed/developed, among which minimizing energy consumption by energy-sensible production scheduling techniques has emerged as a promising one. However, reported workshop models are quite simple and unrealistic. This paper studies a more realistic workshop model called ultra-flexible job shop (uFJS). In an uFJS, the sequence among operations for a job can be changed within certain constraints. To formulate this energy-efficient scheduling problem, a mixed-integer linear programming model was developed. To deal with large-sized problems, a specially designed genetic algorithm (GA) was subsequently proposed and implemented. Numerical results showed the proposed GA worked with decent effectiveness and efficiency. Finally, several comparative studies are carried out to further demonstrate its efficacy in terms of energy efficiency improvement. The advantage of the uFJS as compared to other relative simple workshop models is also shown. By considering the flexibility in operation sequencing in each job, the uFJS effectively integrates process planning and scheduling in discrete parts manufacturing system, thus providing a much larger solution space for more energy-efficient solutions. It therefore provides an excellent platform for decision-makers when developing energy-efficient techniques and strategies

Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA

Ning Xue,Jian-Hua Lin,Shan Xing,Dan Liu,Shi-Bing Li,Yan-Zhen Lai,Xue-Ping Wang,Min-Jie Mao,Qian Zhong,Mu-Sheng Zeng,Wan-Li Liu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.1

Purpose The purpose of this study was to identify novel plasma biomarkers for distinguishing nasopharyngeal carcinoma (NPC) patients from healthy individuals who have positive Epstein-Barr virus (EBV) viral capsid antigen (VCA-IgA). Materials and Methods One hundred seventy-four plasma cytokines were analyzed by a Cytokine Array in eight healthy individuals with positive EBV VCA-IgA and eight patients with NPC. Real-time polymerase chain reaction, Western blotting, enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry were employed to detect the expression levels of macrophage migration inhibitory factor (MIF) and CC chemokine ligand 3 (CCL3) in NPC cell lines and tumor tissues. Plasma MIF and CCL3 were measured by ELISA in 138 NPC patients, 127 EBV VCA-IgA negative (VN) and 100 EBV VCA-IgA positive healthy donors (VP). Plasma EBV VCA-IgA was determined by immunoenzymatic techniques. Results Thirty-four of the 174 cytokines varied significantly between the VP and NPC group. Plasma MIF and CCL3 were significantly elevated in NPC patients compared with VN and VP. Combination of MIF and CCL3 could be used for the differential diagnosis of NPC from VN cohort (area under the curve [AUC], 0.913; sensitivity, 90.00%; specificity, 80.30%), and combination of MIF, CCL3, and VCA-IgA could be used for the differential diagnosis of NPC from VP cohort (AUC, 0.920; sensitivity, 90.00%; specificity, 84.00%), from (VN+VP) cohort (AUC, 0.961; sensitivity, 90.00%; specificity, 92.00%). Overexpressions of MIF and CCL3 were observed in NPC plasma, NPC cell lines and NPC tissues. Conclusion Plasma MIF, CCL3, and VCA-IgA combination significantly improves the diagnostic specificity of NPC in high-risk individuals.

Controlled synthesis of transition metal/conducting polymer nanocomposites

Liu, Zhen,Liu, Yang,Zhang, Lin,Poyraz, Selcuk,Lu, Ning,Kim, Moon,Smith, James,Wang, Xiaolong,Yu, Yajiao,Zhang, Xinyu IOP Pub 2012 Nanotechnology Vol.23 No.33

<P>A novel displacement reaction has been observed to occur between conducting polymers (CP) and metal salts which can be used to fabricate nanostructured CP–metal composites in a one-pot manner. Vanadium pentoxide (V <SUB>2</SUB>O<SUB>5</SUB>) nanofiber is used during the synthesis as the reactive seeds to induce the nanofibril CP–metal network formation. The CP–metal nanocomposites exhibit excellent sensory properties for hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) detection, where both high sensitivity and a low detection limit can be obtained. The sensory performance of the CP–metal composite can be further enhanced by a facile microwave treatment. It is believed that the CP–metal nanofibril network can be converted to a carbon–metal network by a microwave-induced carbonization process and result in the sensory enhancement. </P>

Facile synthesis of polypyrrole coated copper nanowires: a new concept to engineered core–shell structures

Liu, Yang,Liu, Zhen,Lu, Ning,Preiss, Elisabeth,Poyraz, Selcuk,Kim, Moon J.,Zhang, Xinyu The Royal Society of Chemistry 2012 Chemical communications Vol.48 No.20

<P>Polypyrrole coated copper nanowires were synthesized in a one-pot manner in the presence of cupric precursor and pyrrole. They displayed ultra-high sensitivity as a potentiometric sensor with considerable environmental stability comparable to noble metals.</P> <P>Graphic Abstract</P><P>Polypyrrole coated copper nanowires (PPy-CuNWs) were obtained in one-pot synthesis, which possess outstanding chemical sensing properties and environmental stability. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2cc16961d'> </P>