CA-125 cut-off value as a predictor for complete interval debulking surgery after neoadjuvant chemotherapy in patients with advanced ovarian cancer

Naoto Furukawa,Yoshikazu Sasaki,Aiko Shigemitsu,Juria Akasaka,Seiji Kanayama,Ryuji Kawaguchi,Hiroshi Kobayashi 대한부인종양학회 2013 Journal of Gynecologic Oncology Vol.24 No.2

Objective: In the present study, we evaluated changes in CA-125 cut-off values predictive of complete interval debulking surgery (IDS) after neoadjuvant chemotherapy (NAC) using receiver operating characteristic (ROC) analysis. Methods: This retrospective single-institution study included patients with International Federation of Gynecology and Obstetrics (FIGO) stage III epithelial ovarian cancer and a pre-NAC serum CA-125 level of greater than 40 U/mL who were treated with neoadjuvant platinum-based chemotherapy followed by IDS between 1994 and 2009. Logistic regression analysis was used to evaluate univariate and independent multivariate associations with the effect of clinical, pathological, and CA-125 parameters on complete IDS, and ROC analysis was used to determine potential cut-off values of CA-125 for prediction of the possibility of complete IDS. Results: Seventy-five patients were identified. Complete IDS was achieved in 46 (61.3%) patients and non-complete IDS was observed 29 (38.7%). Median pre-NAC CA-125 level was 639 U/mL (range, 57 to 6,539 U/mL) in the complete IDS group and 1,427 U/mL (range, 45 to 10,989 U/mL) in the non-complete IDS group. Median pre-IDS CA-125 level was 15 U/mL (range, 2 to 60 U/mL) in the complete IDS group and 53 U/mL (range, 5 to 980 U/mL) in the non-complete IDS group (p<0.001). Multivariate analyses performed with complete IDS as the endpoint revealed only pre-IDS CA-125 as an independent predictor. The odds ratio of non-complete IDS was 10.861 when the pre-IDS CA-125 level was greater than 20 U/mL. Conclusion: The present data suggest that in the setting of IDS after platinum-based NAC for advanced ovarian cancer, a pre-IDS CA-125 level less than 20 U/mL is an independent predictor of complete IDS.

Parameter dependence of nickel oxide nanoparticles prepared by pulsed-laserablation

Naoto Koshizaki,Takeshi Sasaki,Leszek Zbroniec 한양대학교 세라믹연구소 2005 Journal of Ceramic Processing Research Vol.6 No.2

Nickel oxide nanoparticles were fabricated by a laser ablation technique using the third harmonic of an Nd:YAG laser and sintered NiO targets in an on-axis configuration under argon pressures of 0.67, 1.33, and 2.00 Pa. The effects of the number of irradiating laser pulses, gas pressure, and target-to-substrate (T-S) distance on the average particle size, size distribution, and particle areal density were investigated. The nanoparticles obtained were always crystalline and 2 to 6 nm in diameter, irrespective of the preparation conditions as determined by high-resolution transmission electron microscopic (HRTEM) analysis. The average particle size increased with the number of laser pulses, indicating the particle growth on the substrate. The particle areal density was strongly affected by the target-to-substrate distance. The formation process of the nanoparticles is also discussed.

Near-Field Raman Spectra of GMO Single Crystal

Akira Sakai,Naoto Sasaki,Takanori Ninomiya 한국물리학회 2003 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.42 No.IV

In order to observe the lattice vibration in nano-scale, we have developed a new type of scanning near-eld Raman scattering system by combining scanning atomic force microscopy with microprobed Raman scattering spectroscopy. A special designed cantilever with aperture of about 150 nm in diameter was used both as an illumination nano-source and a nano-detector of scattering light. Near-eld Raman spectra of the 1 internal modes of MoO4 molecules in the GMO single crystal were observed at its cleavage surface at room temperature. The spectrum is compared with that obtained by a conventional Raman microscope. With using the piezoelectric stage, the sample was scanned in nano-scale and near-eld Raman spectra were observed as a function of position on the surface.

Highly Stable Au Nanoparticles with Tunable Spacing and Their Potential Application in Surface Plasmon Resonance Biosensors

Gao, Shuyan,Koshizaki, Naoto,Tokuhisa, Hideo,Koyama, Emiko,Sasaki, Takeshi,Kim, Jae-Kwan,Ryu, Joonghyun,Kim, Deok-Soo,Shimizu, Yoshiki WILEY-VCH Verlag 2010 Advanced Functional Materials Vol.20 No.1

<P>Colloidal Au-amplified surface plasmon resonance (SPR), like traditional SPR, is typically used to detect binding events on a thin noble metal film. The two major concerns in developing colloidal Au-amplified SPR lie in 1) the instability, manifested as a change in morphology following immersion in organic solvents and aqueous solutions, and 2) the uncontrollable interparticle distance, determining probe spacing and inducing steric hindrance between neighboring probe molecules. This may introduce uncertainties into such detecting techniques, degrade the sensitivity, and become the barricade hampering colloidal Au-based transducers from applications in sensing. In this paper, colloidal Au-amplified SPR transducers are produced by using ultrathin Au/Al<SUB>2</SUB>O<SUB>3</SUB> nanocomposite films via a radio frequency magnetron co-sputtering method. Deposited Au/Al<SUB>2</SUB>O<SUB>3</SUB> nanocomposite films exhibit superior stability, and average interparticle distances between Au nanoparticles with similar average sizes can be tuned by changing surface coverage. These characteristics are ascribed to the spacer function and rim confinement of dielectric Al<SUB>2</SUB>O<SUB>3</SUB> and highlight their advantages for application in optimal nanoparticle-amplified SPR, especially when the probe size is smaller than the target molecule size. This importance is demonstrated here for the binding of protein (streptavidin) targets to the probe (biotin) surface. In this case, the dielectric matrix Al<SUB>2</SUB>O<SUB>3</SUB> is a main contributor, behaving as a spacer, tuning the concentration of Au nanoparticles, and manipulating the average interparticle distance, and thus guaranteeing an appropriate number of biotin molecules and expected near-field coupling to obtain optimal sensing performance.</P> <B>Graphic Abstract</B> <P>An innovative colloidal Au-amplified surface plasmon resonance (SPR) transducer is achieved by a using remarkably stable and space-tunable Au/Al<SUB>2</SUB>O<SUB>3</SUB> nanocomposite film. This study indicates that the Au/Al<SUB>2</SUB>O<SUB>3</SUB> nanocomposite film is very promising; it simultaneously overcomes the instability and uncontrollable interparticle distance, which are the current bottlenecks hampering the application of SPR sensors. <img src='wiley_img/1616301X-2010-20-1-ADFM200901232-content.gif' alt='wiley_img/1616301X-2010-20-1-ADFM200901232-content'> </P>

Topological analysis of Au particles in Au/SiO₂ nanocomposite films designed for molecular conduction measurement through Voronoi diagram

Kim, Dae-Gun,Shimizu, Yoshiki,Sasaki, Takeshi,Koshizaki, Naoto,Lee, Byunghoon,Kim, Deok-Soo,Lee, Young Jung,Kim, Young Do IOP Pub 2007 Nanotechnology Vol.18 No.14

<P>Monolayered Au/SiO<SUB>2</SUB> nanocomposite films with a high Au particle number density and insulating property were prepared by radio frequency magnetron co-sputtering to develop a new substrate for molecular conduction measurement. The topologies of Au nanoparticles distributed in the SiO<SUB>2</SUB> matrix were statistically evaluated by morphology observation using a field emission scanning electron microscope (FE-SEM) and the Voronoi diagram of a circle set by regarding the Au nanoparticles as a circle generator. The mean Au particle size and the interparticle distance between neighbours increased with deposition time. However, the fraction of the neighbouring Au nanoparticle combinations having interparticle distance shorter than a certain length increased as the deposition time increased. The results also demonstrated that many conducting paths several tens of nanometres long can be created by attaching conductive molecules 2.4?nm long between the Au nanoparticle combinations. Thus this suggests that the nanocomposite substrate can provide a facile way to measure conducting properties of molecules.</P>

Pulsed-Laser-Induced Simple Synthetic Route for Tb ₃ Al ₅ O ₁₂ :Ce ³⁺ Colloidal Nanocrystals and Their Luminescent Properties

Mhin, SungWook,Ryu, JeongHo,Kim, KangMin,Park, GyeongSeon,Ryu, HanWool,Shim, KwangBo,Sasaki, Takeshi,Koshizaki, Naoto Springer 2009 NANOSCALE RESEARCH LETTERS Vol.4 No.8

<P>Cerium-doped Tb<SUB>3</SUB>Al<SUB>5</SUB>O<SUB>12</SUB>(TAG:Ce<SUP>3+</SUP>) colloidal nanocrystals were synthesized by pulsed laser ablation (PLA) in de-ionized water and lauryl dimethylaminoacetic acid betain (LDA) aqueous solution for luminescent bio-labeling application. The influence of LDA molecules on the crystallinity, crystal morphology, crystallite size, and luminescent properties of the prepared TAG:Ce<SUP>3+</SUP>colloidal nanocrystals was investigated in detail. When the LDA solution was used, smaller average crystallite size, narrower size distribution, and enhanced luminescence were observed. These characteristics were explained by the effective role of occupying the oxygen defects on the surface of TAG:Ce<SUP>3+</SUP>colloidal nanocrystal because the amphoteric LDA molecules were attached by positively charged TAG:Ce<SUP>3+</SUP>colloidal nanocrystals. The blue-shifted phenomena found in luminescent spectra of the TAG:Ce<SUP>3+</SUP>colloidal nanocrystals could not be explained by previous crystal field theory. We discuss the 5d energy level of Ce<SUP>3+</SUP>with decreased crystal size with a phenomenological model that explains the relationship between bond distance with 5d energy level of Ce<SUP>3+</SUP>based on the concept of crystal field theory modified by covalency contribution.</P>

Electronic structure of Li⁺@C₆₀: Photoelectron spectroscopy of the Li⁺@C₆₀[PF₆ ⁻] salt and STM of the single Li⁺@C₆₀ molecules on Cu(111)

Yamada, Yoichi,Kuklin, Artem V.,Sato, Sho,Esaka, Fumitaka,Sumi, Naoto,Zhang, Chunyang,Sasaki, Masahiro,Kwon, Eunsung,Kasama, Yukihiko,Avramov, Pavel V.,Sakai, Seiji Elsevier 2018 Carbon Vol.133 No.-

<P><B>Abstract</B></P> <P>We report the scanning tunneling microscope (STM) observation of the Li<SUP>+</SUP> ion endohedral C<SUB>60</SUB> on Cu(111), prepared by means of evaporation of a high-purity Li<SUP>+</SUP>@C<SUB>60</SUB>[PF<SUB>6</SUB> <SUP>−</SUP>] salt. The electronic state of Li<SUP>+</SUP>@C<SUB>60</SUB> in the Li<SUP>+</SUP>@C<SUB>60</SUB>[PF<SUB>6</SUB> <SUP>−</SUP>] salt was also determined using photoemission and X-ray absorption spectroscopy, along with the density functional theory (DFT) calculations. In the salt, Li and PF<SUB>6</SUB> had nearly single positive and negative charge, respectively; thus the C<SUB>60</SUB> cage was practically neutral. The salt decomposed under ultra-high vacuum while heating at 400 °C. This allowed the selective deposition of Li<SUP>+</SUP>@C<SUB>60</SUB> on Cu(111). Although secondary-ion mass spectroscopy of the deposited Li<SUP>+</SUP>@C<SUB>60</SUB> film showed a decrease in the Li-content during evaporation, Li<SUP>+</SUP>@C<SUB>60</SUB> was successfully identified using STM. The DFT calculations of Li<SUP>+</SUP>@C<SUB>60</SUB> on Cu(111) suggested that the Li<SUP>+</SUP> ion was singly charged and the location of the Li<SUP>+</SUP> ion was displaced in an upward direction, which altered the local density of states in an upper section of C<SUB>60</SUB>, especially for LUMO+2. The calculated results were mostly in agreement with the bias-dependent STM and dI/dV images. However, an inconsistency was observed between the calculation and experiments in case of empty state imaging where tip-induced displacement of the Li<SUP>+</SUP> ion may occur.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Hepatoprotective Effects of Potato Peptide against D-Galactosamineinduced Liver Injury in Rats

Kiyoshi Ohba,Kyu-Ho Han,Ruvini Liyanage,Megumi Nirei,Naoto Hashimoto,Ken-ichiro Shimada,Mitsuo Sekikawa,Keiko Sasaki,Chi-Ho Lee,Michihiro Fukushima 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.6

The effect of some peptides on hepatoprotection and cecal fermentation against D-galactosamine (GalN)-treated rats was studied. In acute hepatic injury tests, serum alanine aminotransferase (ALT), aspartate aminotranferase (AST), and lactic dehydrogenase (LDH) activities were remarkably increased after injection of GalN. However, potato and soybean peptides significantly decreased GalN-induced alterations of serum ALT and AST activities. Hepatic thiobarbituric acidreactive substance (TBARS) concentration in GalN-treated groups fed potato and soybean peptides was significantly lower than that in GalN-treated control group. Hepatic glutathione level in the GalN-treated group fed potato peptide was significantly higher than that in GalN-treated control group. Furthermore, cecal Lactobacillus level in GalN-treated groups fed potato and soybean peptides was significantly higher than that in GalN-treated control group, and cecal short-chain fatty acid concentrations in GalN-treated group fed potato peptide were significantly higher than in GalN-treated control group. These results indicate that potato peptide may improve the cecal fermentation and prevent the GalN-induced liver damage in rats.

Simple synthetic route for hydroxyapatite colloidal nanoparticles via a Nd:YAG laser ablation in liquid medium

Mhin, Sung Wook,Ryu, Jeong Ho,Kim, Kang Min,Park, Gyeong Seon,Ryu, Han Wool,Shim, Kwang Bo,Sasaki, Takeshi,Koshizaki, Naoto Springer-Verlag 2009 APPLIED PHYSICS A MATERIALS SCIENCE AND PROCESSING Vol.96 No.2