혈청 Hepatitis B Virus DNA의 검출

최명재 서강정보대학 1992 論文集 Vol.11 No.-

Radiological molecular hybridization test was employed to detect hepatitis B virus (HBV) DNA in human serum. HBV-DNA was determined in 136 serum samples by the method, and the results were compared to that obtained with radioimmunoassay(RIA) for serological HBV markers. From total 136 HBsAg positive cases, serum HBV-DNA was detected in 92 (67.6%) cases. HBV-DNA was shown in 71 of 83 (85.5%) HBeAg positive cases and 16 of 43 (37.1%) anti-HBe positive cases, 5 of 10 (50%) negative of both HBeAg and anti-HBe. Among 92 HBV-DNA positive cases, HBeAg positive cases showed higher HBV-DNA than anti-HBe positive cases. These results indicate that radiological molecular hybridization test is sensitive and accurate method to detect hepatitis B virus DNA in patient serum.

Rhodopseudomonas sphaeroides의 Uptake Hydrogenase에 관한 연구

최명재,강대길 서강정보대학 1990 論文集 Vol.9 No.-

We study the effect of uncouplers, which inhibite phosphorylation and inhibitors, which block electron transport in specific sites of electron transport chain, on hydrogenase activity from whole cell Rhodopseudomonas sphaeroides. In the presence of 10μM 2,4-DNP and CCCP as uncouplers, and 10μM rotenone which blocks between NADH₂ and ubiquinone, 5μM antimycin A which blocks between cytochrome b and c in the electron transport system, the uptake hydrogenase activities change into 95%, 102%, 96%, 37% of normal hydrogenase activity in resting cell. These result show that the hydrogenase coupling which ubiquinone in electron transport chain and ATP make an important role in hydrogenase synthesis.

모험기업의 라이프 싸이클 단계별 재무전략

김재명,오정화,손재영,최용훈 강원대학교 석재복합신소재제품연구센터 1997 석재연 논문집 Vol.2 No.-

본 논문은 신제품의 사업화와 관련한 모험기업의 라이프 싸이클 단계별 재무전략, 특히 자금조달 전략에 관해 연구하였다. 특히 금융상품의 유형과 특성을 자본원천별로 분석하고, 이에 기초하여 모험기업의 성장단계별 자금조달전략을 제시하였다. 본 논문은 라이프싸이클을 4단계로 나누고, 각 단계별 재무특성과 자금원천의 특성을 고려하여 라이프싸이클 단계별 자금조달원천 행렬표를 제시하였다. 이에 따르면 모험기업은 라이프싸이클 단계별로 개인저축, 에인젤, 모험자본, 동업자, 기업공개, 그리고 잉여금 등의 지분자본원천과 회사채, 거래신용, 자산기초금융, 은행이나 보험회사 차입, 그리고 정책지원자금 등 부채자본원천을 중첩하여 자본조달원천으로 사용할 수 있음을 보여주고 있다. This paper discusses the relationships between stage of development in venture business life cycles and financial strategies. The paper selectively examines the study by reviewing the characteristics of the various finacial instruments on the basis of the cash flows of venture business life cycles. I suggested the source of capital matrix combining the various sources of capital. At each stage, the venture businessess should raise the funds by using the various combinations of sources of equity financing and debt financing.

석재복합제품 관련산업의 신제품 개발전략

김재명,손재영,최용훈,오정화 강원대학교 석재복합신소재제품연구센터 1998 석재연 논문집 Vol.3 No.-

본 연구는 신제품 개발이론에 대한 이론적 틀을 연구하고, 이에 기초하여 석재복합 관련제품의 신제품 개발 노선, 기술, 신제품 개발전략 구축과 선택 방안을 제시하였다. 석재복합 관련 제품의 대부분은 첨단 고도기술을 필요로 하는 영역이 한정되어 있기 때문에 이 산업 분야에서의 신제품 개발은 신제품 개발계획과 지침을 마련할 필요가 있다. 또한 유망 신제품 안건으로 계층화하여 신제품 개발의 기본전략을 구축하는 신규사업 시나리오를 작성하여 신제품 개발전략을 구축하는 것이 효과적이다. 이러한 과정을 거쳐 중소기업의 경우는 현존하는 시장, 제품, 그리고 기술우위에 기반을 둔 신제품을 개발할 경우 성공 가능성이 클 것이다. 이 이외에도 석재복합제품 개발은 석재자원과 신소재 개발에 기초한 신제품 개발에 필요한 기술 진전 사항, 즉 기술개발-제품개발-시장진입 활동간 상호 연계성을 고려해야 한다. This study suggests the theoretical framework and strategy of new product development in the avdanced mineral aggregate composite industry. The advanced mineral aggregate composite area needs to prepare the plan and guidelines on the new product development owing to the characteristic of the related technology. Alternative scenarios on the hierachy of new product idea is effective to the success of new product development strategy. Especially, the existing market, product, and the technological advantages affects the possibility of the success of new product development of the small firms. Also considering the interrelationship between the technological trends is important to the new product development of advanced mineral aggregate composite industry.

도암호의 수질과 어류군집 특성 연구

이상하,최재석,이광열,장영수,임인수,허우명,김재구,김범철 강원대학교 부속 환경연구소 2005 Journal of the Environment Vol.2 No.-

To investigate nutrient dynamics of the lake and fish community, both water and fish samples were collected at three different areas (dam site, upper inflowing and downstream of lake outfow) for one year from Sep.2004 to Aug.2005 During the experimental periods, mean concentration of chl, a in epilimnetic layer (0-5m) was 18.5mg^(-3) and transparency ranged from 0.3m to 2.4m, respectively. In nutrient, mean concentrations of TP and TN were 111 mgP m^(-3) and 4.4 mgN L^(-1), respectively. Based on the water quality standard suggested by U.S.EPA(*1976), the trophic state of Lake Doam was classified as eutrophic state. Total number of fish collected in Lake Doam was 9,600 indiveduals in 26 species of 6 family. Sixteen of Korean endemic soecies including H. mylodon, A. signifer, P. tenuicorpa occurred. O. mykiss as introduced fish was also found in Lake Doam. Dominant and subdominant species were P. herzi and Z. platypus that took about 34.6% and 22.5% of total fish composition, respectively. Six species including A. signifer. P. tenuicorpa. and R. kumgangensis were rare in Lake Doam. Pelagic typw of figh commonly occurred at upper regions, whereas benthic type was mainly domenated at downstream area. Therefore, pollution source inflowed from the upper regions did not seriously affect downstream area (Songcheon water system) due to the role of dam as pollutant barrier. In addition high appearance of Comat type of fish that si hybrid between gold fish (C.auratus) and C. auratus was found in the lake, It was unclear the reasons that high prorortion of mutant species apperared in the lake. Further more researches are required in this area.

Randomized, Controlled, Multi-center Trial: Comparing the Safety and Efficacy of DA-9701 and Itopride Hydrochloride in Patients With Functional Dyspepsia

( Myung Gyu Choi ),( Poong Lyul Rhee ),( Hyojin Park ),( Oh Young Lee ),( Kwang Jae Lee ),( Suck Chei Choi ),( Sang Young Seol ),( Hoon Jai Chun ),( Jong Sun Rew ),( Dong Ho Lee ),( Geun Am Song ),( H 대한소화기기능성질환·운동학회 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.3

Background/Aims: Therapies of functional dyspepsia (FD) are limited. DA-9701 is a novel prokinetic agent formulated with Pharbitis semen and Corydalis Tuber. We aimed to assess the efficacy of DA-9701 compared with itopride in FD patients. Methods: Patients with FD randomly received either itopride 50 mg or DA-9701 30 mg t.i.d after a 2-week baseline period. After 4 weeks of treatment, 2 primary efficacy endpoints were analyzed: the change from baseline in composite score of the 8 dyspeptic symptoms and the overall treatment effect. Impact on patients`` quality of life was assessed using the Nepean Dyspepsia Index (NDI) questionnaire. Results: We randomly assigned 464 patients with 455 having outcome data. The difference of the composite score change of the 8 symptoms between the 2 groups was 0.62, indicating that DA-9701 was not inferior to itopride. The overall treatment effect response rate was not different between the groups. When responder was defined as ≥ 5 of the 7 Likert scale, responder rates were 37% of DA-9701 and 36% of itopride group. Patients receiving DA-9701 experienced similar mean percentage of days with adequate relief during the 4-week treatment period compared with those receiving itopride (56.8% vs 59.1%). Both drugs increased the NDI score of 5 domains without any difference in change of the NDI score between the groups. The safety profile of both drugs was comparable. Conclusions: DA-9701 significantly improves symptoms in patients with FD. DA-9701 showed non-inferior efficacy to itopride with com - parable safety. (J Neurogastroenterol Motil 2015;21:414-422)

Expression and Characterization of Thiol-specific Antioxidant Protein, DirA of Corynebacterium diphtheriae

Choi,Myung-Jai,Choi,Won-Ki,Kim,Kanghwa THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1998 Journal of biomedical laboratory sciences Vol.4 No.1

효모의 티올특이성 항산화단백과 아미노산 서열상 상동성을 보이는 50 종류의 단백은 새로운 항산화 단백군을 형성하며 또한 병원성 미생물에도 널리 분포하고 있으나 이들 단백의 행화학적 및 생리적인 기능은 거의 알려져 있지 않은 실정이다. 본 연구는 병원성 미생물의 티올특이성 항산화단백의 기능에 관한 연구로서 Saccharomyces cerevisiae의 TSA 및 Salmonella typhimurium alkyl hydroperoxide reductase의 AhpC subunit와 상동성를 나타내는 Corynebacterium diphtheriae의 DirA 유전자를 PCR 방법으로 클로닝하고 대장균에 발현시킨 후 정제하여 항산화 특성을 조사하였다. 정제된 DirA는 티올을 함유하는 금속촉매 산화계인 DTT/Fe³+를 선택적으로 억제하였으며 티오레독신 의존성 과산화물 분해활성을 나타내었다. DTT/Fee³+ 금속촉매 산화계에 의한 효소의 불활성화를 50% 억제하는 DirA의 농도는 0.12 mg/ml로 효모 TSA 항산화활성의 약1/4 수준이었으며, 효모의 티오레독신계와 반응시켰을 때 과산화물 분해활성은 0.02 unit/mg로서 TSA의 티오레독신 의존성 과산화물 분해활성의 1/20 수준이었다. 정제된 단백질을 이용하여 항체를 제조하였으며 이항체를 이용하여 Corynebacterium diphtheriae에서 발현됨을 확인하였다. 이러한 결과를 통하여 Corynebacterium diphtheriae의 병원성은 숙주세포의 방어기전인 백혈구에 의하여 생성되는 과산화수소 또는 다른 활성산소종을 제거하는 DirA작용과 연관이 있는 것으로 사료된다. A Corynebacterium diphtheriae iron-repressible gene dirA, that was homologous to TSA of Saccharomyces cerevisiae and AhpC subunit of Salmonella typhimurium alkyl hydroperoxide reductase, was amplified with PCR and expressed in E. coli. The DirA purified from the transformed E. coli crude extracts prevented the inactivation of enzyme caused by metal-catalyzed oxidation (MCO) system containing thiols but not by ascorbate/Fe³+/O₂MCO system. The DirA concentration, which inhibited the inactivation of glutamine synthetase by 50%(IC50) against MCO system, was 0.12 mg/ml. The multimeric forms of DirA were converted to the monomeric form in SDS-PAGE under the thioredoxin system comprised of NADPH, Saccharomyces cerevisiae thioredoxin reductase, and thioredoxin. Also, DirA showed thioredoxin dependent peroxidase activity. All of these results were consistent with the characteristics of a thiol specific antioxidant (TSA) protein having two conserved cysteine residues.

Alterations in Blood Electrolyte of Rabbits with Experimental Injection of Escherichia coli Endotoxin

Choi, Seok-Cheol,Kim, Jai-Young,Kwon, Heun-Young,Kim, Tae-Un,Hwang, Soo-Myung,Lee, Won-Jae 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.2

혈액 전해질 성분들에 대한 대장균 내독소의 영향을 조사하기 위해 토끼를 대상으로 한 동물실험을 실시하였다. 대장균 내독소(혈청형 O55: B5)를 토끼의 귀정맥을 통해 0.10mg/kg 혹은 0.50mg/kg농도로 주입한 후 3,6,12,24시간대에 채혈하여서 Ca++,Mg++, K+, CI- 농도를 측정하였다. 대조군에 비해, 내독소투여 토끼의 Ca++농도는 6시간대에 증가하였고, Mg++농도는 3,6,12시간대에, Na++과 K+는 모든 채혈시간대에, 그리고 CI- 농도는 3,12,24시간대에 각각 유의하게 높았다(p<0.05). 흥미롭게도, 고 Mg+혈증(약 4.0mg/dL)을 보인 내독소투여 토끼들은 심각한 임상징후들로 인식되는 분비물의 증가, 쇼크, 빈호흡, 경련, 혹은 설사와 같은 증세를 보였다. 본 연구의 결과들은 대장균 내독소가 혈액 전해질 농도의 항상성 혼란을 유도하여 이러한 생리적 불균형은 치명적 상황과 그로인한 죽음을 야기할 수도 있음을 시사하고 있다. We studied the effects of Escherichia coli(E, coli) endotoxin on blood electrolytes levels in rabbits. Endotoxin(E, coli serotype O55: B5) was injected via rabbits' ear vein: 0.10mg/kg(Group A) or 0.50 mg/kg(Group B). Blood samples were taken at postendotoxemic 3, 6,12 and 24 hrs and were analyzed for detections of the levels of blood electrolytes such as Ca++, Mg++,Na+, K+ and CI-. As compared to control group, in endotoxin-treated rabbits Ca++, levels elevated at 6 hrs but decreased at 24 hrs, Mg++ levels were high at 3,6 and 12 hrs, Na+ and K+levels increased at all sampling times and CI- levels rose at 3, 12 and 24 hrs (p<0.05). Interestingly, endotoxic rabbits having hypermagnesemia (about 4.0 mg/dL) showed severe syndromes such as increased secretion, shock, tachypnea, seizure and/or diarrhea, suggesting that these may be clinical signs of imminent death in rabbits. These observations testify that bacterial endotoxin leads to dyshomeostasis of blood electrolytes and the physiological imbalances may cause fatal disorders and subsequent death.

Expression and Characterization of Thiol-Specific Antioxidant Protein, DirA of Corynebacterium diphtheriae

Myung-Jai Cho(최명재),Kanghwa Kim(김강화),Won-Ki Choi(최원기) 대한의생명과학회 1998 Biomedical Science Letters Vol.4 No.1

효모의 티올특이성 항산화단백과 아미노산 서열상 상동성을 보이는 50 종류의 단백은 새로운 항산화 단백군을 형성하며 또한 병원성 미생물에도 널리 분포하고 있으나 이들 단백의 생화학적 및 생리적인 기능은 거의 알려져 있지 않은 실정이다. 본 연구는 병원성 미생물의 티올특이성 항산화 단백의 기능에 관한 연구로서 Saccharomyces cerevisiae의 TSA 및 Salmonella typhimurium alkyl hydroperoxide reductase의 AhpC subunit와 상동성을 나타내는 Corynebacterium diphtheriae의 DirA 유전자를 PCR 방법으로 클로닝하고 대장균에 발현시킨 후 정제하여 항산화 특성을 조사하였다. 정제된 DirA는 티올을 함유하는 금속촉매 산화계인 DTT/F³?를 선택적으로 억제하였으며 티오레독신 의존성 과산화물 분해활성을 나타내었다. DTT/F³? 금속촉매 산화계에 의한 효소의 불활성 화를 50% 억제하는 DirA의 농도는 0.12 ㎎/㎖로 효모 TSA 항산화활성 의 약¼ 수준이었으며, 효모의 티오레독신계와 반응시켰을 때 과산화물 분해활성은 0.02 unit/㎎로서 효모 TSA의 티오레독신 의존성 과산화물 분해활성의 1/20 수준이었다. 정제된 단백질을 이용하여 항체를 제조하였으며 이항체를 이용하여 Corynebacterium diphtheriae에서 발현됨을 확인하였다. 이러한 결과를 통하여 Corynebacterium diphtheriae의 병원성은 숙주세포의 방어기전인 백혈구에 의하여 생성되는 과산화수소 또는 다른 활성산소종을 제거하는 DirA작용과 연관이 있는 것으로 사료된다. A Corynebacterium diphtheriae iron-repressible gene dirA, that was homologous to TSA of Saccharomyces cerevisiae and AhpC subunit of Salmonella typhimurium alkyl hydroperoxide reductase, was amplified with PCR and expressed in E. coli. The DirA purified from the transformed E. coli crude extracts prevented the inactivation of enzyme caused by metal-catalyzed oxidation (MCO) system containing thiols but not by ascorbate/Fe³?/O₂ MCO system. The DirA concentration, which inhibited the inactivation of glutamine synthetase by 50% (IC??) against MCG system, was 0.12 ㎎/㎗. The multimeric forms of DirA were converted to the monomeric form in SDS-PAGE under the thioredoxin system comprised of NADPH, Saccharomyces cerevisiae thioredoxin reductase, and thioredoxin. Also, DirA showed thioredoxin dependent peroxidase activity. All of these results were consistent with the characteristics of a thiol specific antioxidant (TSA) protein having two conserved cysteine residues.

Molecular Cloning and Analysis of Transcription Initiation in the Anagrapha faicifera Multi Nucleocapsid polyhedrosis Virus Polyhedrin Gene

Choi, Ju Young,Kim, Young Lim,Yang, Jai Myung Korean Society of Molecular Biology 1998 Molecules and cells Vol.8 No.5