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Screening for drought tolerance in wheat genotypes by morphological and SSR markers
Muhammad Shahidul Haque,Nihar Ranjan Saha,Muhammad Tariqul Islam,Muhammad Monirul Islam,권수정,ROY SWAPAN KUMAR,우선희 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.1
Drought stress has become a significant abiotic stress for wheat in Bangladesh. This study aimed to explore drought tolerance in Bangladeshi wheat based on morpho-physiological and molecular traits. Ten wheat genotypes selected based on the previous research were grown in pots under control (100% field capacity) and drought stress (30% field capacity), applied from third leaf stage till maturity. Drought stress aff ected the yield contributing characters and significantly reduced grain yield. Drought susceptibility index and drought tolerance efficiency were used as criteria for selection. Sonalika, Sourav, and BARI gom-28 were least affected and emerged as drought tolerant. Durum, Pavon-76, BARIgom-25, and BARIgom-33 were moderately tolerant, while BARIgom-24, BINAgom-1, and BARIgom-20 were susceptible as they were severely affected. Thirteen SSR markers linked to drought tolerance were used to characterize 26 wheat genotypes. A total of 73 alleles were detected with an average of 5.62 alleles per locus. Polymorphism information content (PIC) and Nei’s(Proc Natl Acad Sci USA 70:3321–3323; 1973) gene diversity were 0.69 and 0.73, respectively. The coefficient of genetic similarity matrix for all 325 pairs of genotypes ranged from 0.15 to 1.00 with an average of 0.57. Similarity indices-based cluster analysis separated 26 genotypes into different groups. Two tolerant genotypes, Sourav and Sonalika, and two moderately tolerant genotypes, Durum and Pavon-76, as identified by morphological study were grouped in cluster 1. These four genotypes, therefore, passed the screening for drought tolerance in molecular and morphological studies and are promising for cultivation in the drought-prone areas of Bangladesh.
Mohammad Tariqul Islam,심재진,유바라즈할도라이,웬방호아,Muhammad Naoshad Islam,나춘섭 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.6
Poly(vinyl acetate) (PVAc) was synthesized by the atom transfer radical polymerization of vinyl acetate(VAc) in supercritical carbon dioxide using CuBr/2,2':6',2''-terpyridine complex as a catalyst and ethyl 2-bromoisobutyrateas an initiator. Polymerization proceeded in a controlled manner, and low to moderate conversion wasachieved within a reasonable time. The effects of the monomer amount, temperature, pressure, initiator, and ligandloading on monomer conversion, molecular weight and molecular weight distribution of the polymer were examined. The reaction kinetics was also investigated. The polymerization reaction was found to be first-order with respect to themonomer concentration. The molecular weights of the resulting PVAc increased linearly with increasing VAc conversion.
Muhammad Omer,박수영,Mohammad Tariqul Islam,Mashooq Khan,김영규,이준형,강인규 한국고분자학회 2014 Macromolecular Research Vol.22 No.8
The interface between a nematic liquid crystal phase, 4-cyano-4′-pentylbiphenyl (5CB) and water wasexamined for protein detection by monitoring the formation of a complex between sodium polystyrene sulfonate(PSSNa) and a positively charged biological species on the 5CB in a transmission electron microscopy (TEM) gridcell coated with a strong anionic polyelectrolyte-containing block copolymer, LCP-b-PSSNa (LCP:poly(4-cyanobiphenyl-4′-oxyundecylacrylate)). This block copolymer was successfully synthesized by reversible addition-fragmentationchain transfer polymerization. A monolayer of LCP-b-PSSNa in a Langmuir Blodgett trough (in whichPSSNa and LCP were located in and above water, respectively, in the TEM grid cell) was transferred to the 5CB/water interface in the 5CB-filled TEM grid that was already placed on octadecyltrichlorosilane-coated glass. Modelproteins such as bovine serum albumin (BSA), hemoglobin (Hb), α chymotrypsinogen-A (ChTg), and lysozyme(LYZ) having different isoelectric points (pIs) were tested for non-specific protein detection. When the protein solutionswere injected into the TEM grid cell, the initial homeotropic orientation of 5CB in the TEM grid cell changedto a planar one below the pIs of the proteins due to electrostatic interactions between PSSNa (- charge) and the proteins(+ charge); this did not occur above the pIs of the tested proteins. The minimum concentrations at which thehomeotropic to planar configurational changes (H-P changes) occurred were 0.02, 0.04, 0.04, and 0.08 wt% forBSA, Hb, ChTg, and LYZ, respectively. Therefore, the positively charged biomaterials were visually detected at thePSSNa-coated LC/water interface during an H-P change by using polarized optical microscopy under crossed polarizers. This simple set-up for non-specific biomaterial detection paves a way for the development of efficient andexcellent quality biosensors.