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RISS 인기검색어
- 검색키워드
- 저자 : Nihar Ranjan Saha (검색결과 3 건)
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Screening for drought tolerance in wheat genotypes by morphological and SSR markers
Muhammad Shahidul Haque,Nihar Ranjan Saha,Muhammad Tariqul Islam,Muhammad Monirul Islam,권수정,ROY SWAPAN KUMAR,우선희 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.1
Drought stress has become a significant abiotic stress for wheat in Bangladesh. This study aimed to explore drought tolerance in Bangladeshi wheat based on morpho-physiological and molecular traits. Ten wheat genotypes selected based on the previous research were grown in pots under control (100% field capacity) and drought stress (30% field capacity), applied from third leaf stage till maturity. Drought stress aff ected the yield contributing characters and significantly reduced grain yield. Drought susceptibility index and drought tolerance efficiency were used as criteria for selection. Sonalika, Sourav, and BARI gom-28 were least affected and emerged as drought tolerant. Durum, Pavon-76, BARIgom-25, and BARIgom-33 were moderately tolerant, while BARIgom-24, BINAgom-1, and BARIgom-20 were susceptible as they were severely affected. Thirteen SSR markers linked to drought tolerance were used to characterize 26 wheat genotypes. A total of 73 alleles were detected with an average of 5.62 alleles per locus. Polymorphism information content (PIC) and Nei’s(Proc Natl Acad Sci USA 70:3321–3323; 1973) gene diversity were 0.69 and 0.73, respectively. The coefficient of genetic similarity matrix for all 325 pairs of genotypes ranged from 0.15 to 1.00 with an average of 0.57. Similarity indices-based cluster analysis separated 26 genotypes into different groups. Two tolerant genotypes, Sourav and Sonalika, and two moderately tolerant genotypes, Durum and Pavon-76, as identified by morphological study were grouped in cluster 1. These four genotypes, therefore, passed the screening for drought tolerance in molecular and morphological studies and are promising for cultivation in the drought-prone areas of Bangladesh.
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Genetic Diversity Analysis and Molecular Screening for Salinity Tolerance in Wheat Germplasm
( Muhammad Abdul Hannan ),( Nihar Ranjan Saha ),( Swapan Kumar Roy ),( Sun-hee Woo ),( Muhammad Shahidul Haque ) 한국육종학회 2021 Plant Breeding and Biotechnology Vol.9 No.3
Salinity is the most significant constraint to wheat cultivation in the salinity-affected areas. Saline-prone soils can be brought under cultivation by the development of salt-tolerant lines through molecular breeding aided by diversity analysis and molecular screening. The study aimed to screen for salinity tolerance and elucidate the extent of diversity among 75 wheat varieties and accessions. This molecular analysis and detection used 21 linked SSR markers. The study identified 202 alleles in 75 genotypes of wheat, giving 9.6 alleles on an average for salt tolerance screening. The mean polymorphism information content (PIC) of 0.7599 and the Nei’s (1973) gene diversity of 0.7856 were recorded. The accessions possess higher gene diversity and PIC than the cultivars. The average genetic similarity matrix coefficient was 0.60 within the range of 0.19 and 1.00. Similarity indices based UPGMA cluster analysis separated the 75 germplasm into six clusters. Eight genotypes grouped in sub-cluster Ib together with the positive control BINA Gom-1. Therefore, these eight genotypes were identified as potential salt-tolerant by molecular analysis using SSR markers linked to salinity. The gene diversity estimated, and the germplasm identified as potential salt-tolerant are promising for use in wheat breeding to incorporate salt tolerance in novel cultivars by marker-assisted breeding.
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Predictive factors for malignancy in undiagnosed isolated small bowel strictures
( Ujjwal Sonika ),( Sujeet Saha ),( Saurabh Kedia ),( Nihar Ranjan Dash ),( Sujoy Pal ),( Prasenjit Das ),( Vineet Ahuja ),( Peush Sahni ) 대한장연구학회 2017 Intestinal Research Vol.15 No.4
Background/Aims: Patients with small bowel strictures have varied etiologies, including malignancy. Little data are avail able on the demographic profiles and etiologies of small bowel strictures in patients who undergo surgery because of intestinal obstruction but do not have a definitive pre-operative diagnosis. Methods: Retrospective data were analyzed for all patients operated between January 2000 and October 2014 for small bowel strictures without mass lesions and a definite diagnosis after imaging and endoscopic examinations. Demographic parameters, imaging, endoscopic, and histological data were extracted from the medical records. Univariate and multivariate analyses were conducted to identify factors that could differentiate be tween intestinal tuberculosis (ITB) and Crohn’s disease (CD) and between malignant and benign strictures. Results: Of the 7,425 reviewed medical records, 89 met the inclusion criteria. The most common site of strictures was the proximal small intes tine (41.5%). The most common histological diagnoses in patients with small bowel strictures were ITB (26.9%), CD (23.5%), non-specific strictures (20.2%), malignancy (15.5%), ischemia (10.1%), and other complications (3.4%). Patients with malignant strictures were older than patients with benign etiologies (47.6±15.9 years vs. 37.4±16.4 years, P=0.03) and age >50 years had a specificity for malignant etiology of 80%. Only 7.1% of the patients with malignant strictures had more than 1 stricture and 64% had proximally located strictures. Diarrhea was the only factor that predicted the diagnosis of CD 6.5 (95% confidence interval, 1.10-38.25; P=0.038) compared with the diagnosis of ITB. Conclusions: Malignancy was the cause of small bowel strictures in approximately 16% patients, especially among older patients with a single stricture in the proximal location. Empirical therapy should be avoided and the threshold for surgical resection is low in these patients. (Intest Res 2017;15:518-523)
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