Quantitative trait loci analysis of morphological traits in Citrus

Mehtap S¸ ahin-C¸ evik,Gloria A. Moore 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.1

The objectives of this study were to understand the genetic basis of morphological variation observed in the genus Citrus and its relatives and to identify genomic regions associated with certain morphological traits using genetic linkage mapping and quantitative trait loci (QTLs) analysis with random amplified polymorphic DNA (RAPD) markers. First, a genetic linkage map was constructed with RAPD markers obtained by screening 98 progeny plants from a {Citrus grandis 9 [C. paradisi 9 Poncirus trifoliata]} 9 {[(C. paradisi 9 P. trifoliata) 9 C. reticulata] 9 [(C. paradisi 9 Poncirus trifoliata) 9 C. sinensis]} intergeneric cross. The map contains 69 RAPD markers distributed into nine linkage groups. Then, 17 different morphological traits, including six tree and two leaf characters of 98 progeny plants and six floral and three fruit characters of about half of the same progeny plants were evaluated for 2 years and statistically analyzed for variation. Statistical analysis of individual traits indicated that trunk diameter and growth, tree height, canopy width, tree vigor and growth, leaf length and width, petal and anther numbers, petal length and width, length of pistil and style, fruit length and diameter, and fruit segment number showed normal or close to normal distribution, suggesting that these traits may be inherited quantitatively. Quantitative data from the morphological traits were analyzed to detect markers and putative QTLs associated with these traits using interval mapping method. QTL analysis revealed 18 putative QTLs of LOD[3.0 associated with 13 of the morphological traits analyzed. The putative QTLs were distributed in several different linkage groups, and QTLs associated with similar traits were mostly mapped to the same LG or similar locations in the linkage group, indicating that the same genomic region is involved in the inheritance of some of the morphological traits.

Distribution of stress state in the Nankai subduction zone, southwest Japan and a comparison with Japan Trench

Lin, W.,Byrne, T.B.,Kinoshita, M.,McNeill, L.C.,Chang, C.,Lewis, J.C.,Yamamoto, Y.,Saffer, D.M.,Casey Moore, J.,Wu, H.Y.,Tsuji, T.,Yamada, Y.,Conin, M.,Saito, S.,Ito, T.,Tobin, H.J.,Kimura, G.,Kanagaw Elsevier Scientific Publishing Co 2016 Tectonophysics Vol.692 No.2

<P>To better understand the distribution of three dimensional stress states in the Nankai subduction zone, southwest Japan, we review various stress-related investigations carried out in the first and second stage expeditions of the Nankai Trough Seismogenic Zone Experiment (NanTroSEIZE) by the Integrated Ocean Drilling Program (IODP) and compile the stress data. Overall, the maximum principal stress sigma(l) in the shallower levels (<similar to 1 km) is vertical from near the center of forearc basin to near the trench and; the maximum horizontal stress S-Hmax (interpreted to be the intermediate principal stress sigma(2)) is generally parallel to the plate convergence vector. The exception to this generalization occurs along the shelf edge of the Nankai margin where S-Hmax, is along strike rather than parallel to the plate convergence vector. Reorientation of the principal stresses at deeper levels (e.g., >similar to 1 km below seafloor or in underlying accretionary prism) with sigma(1) becoming horizontal is also suggested at all deeper drilling sites. We also make a comparison of the stress state in the hanging wall of the frontal plate-interface between Site C0066 in the Nankai and Site C0019 in the Japan Trench subduction zone drilled after the 2011 Mw 9.0 Tohoku-Oki earthquake. In the Japan Trench, a comparison between stress state before and after the 2011 mega-earthquake shows that the stress changed from compression before the earthquake to extension after the earthquake. As a result of the comparison between the Nankai Trough and Japan Trench, a similar current stress state with trench parallel extension was recognized at both C0006 and C0019 sites. Hypothetically, this may indicate that in Nankai Trough it is still in an early stage of the interseismic cycle of a great earthquake which occurs on the decollement and propagates to the toe (around site C0006). (C) 2015 Elsevier B.V. All rights reserved.</P>

MAOIs : A Practical Guide to Their Use

Moore, C. Medical Publications Korea 1985 Medical Progress Vol.7 No.4

교대근무자의 인적과실 방지와 건강관리

C. Moore-Ede Martin 한국원자력산업회의 1987 원자력산업 Vol.7 No.10

오늘날 점점 더 많은 사람들이 밤샘을 하게 되었고 밤과 저녁 근무는 점점 더 보편화 돼 가고 있으며 낮근무만 하는 직장을 구하기가 점점 더 어려워지고 있다. 교대근무자들은 수면, 소화기능, 가족, 사회생활 등에 얽힌 문제점들을 어떻게 풀어나갈 것인가.

Carcass Characteristics and Meat Quality of Swamp Buffaloes (Bubalus bubalis) Fattened at Different Feeding Intensities

Lambertz, C.,Panprasert, P.,Holtz, W.,Moors, E.,Jaturasitha, S.,Wicke, M.,Gauly, M. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4

Twenty-four male 1-year old swamp buffaloes (Bubalus bubalis) were randomly allocated to 4 groups. One group grazed on guinea grass (GG) and another on guinea grass and the legume Stylosanthes guianensis (GL). The other two groups were kept in pens and fed freshly cut guinea grass and concentrate at an amount of 1.5% (GC1.5) and 2.0% (GC2.0) of body weight, respectively. The effect of the different feeding intensities on carcass characteristics and meat quality were assessed. The mean body weight at slaughter was 398 (${\pm}16$) kg. Average daily gain was higher in concentrate-supplemented groups (570 and 540 g/d in GC1.5 and GC2.0, respectively) when compared to GG (316 g/d) and GL (354 g/d) (p<0.01). Likewise, the warm carcass weight was higher in GC1.5 and GC2.0 compared to GG and GL. Dressing percentage was 48.1% and 49.5% in GC1.5 and GC2.0 in comparison to 42.9% and 44.8% observed in GG and GL, respectively. Meat of Longissimus throracis from GC1.5 and GC2.0 was redder in color (p<0.01), while water holding capacity (drip and thawing loss) was improved in pasture-fed groups (p<0.05). Protein and fat content of Longissimus thoracis was higher in animals supplemented with concentrate (p<0.01), as was cholesterol content (p<0.05), whereas PUFA:SFA ratio was higher and n-6/n-3 ratio lower (p<0.01) in pasture-fed buffaloes. Results of the present study showed that the supplementation of pasture with concentrate enhances the growth and carcass characteristics of swamp buffaloes expressed in superior dressing percentage, better muscling, and redder meat with a higher content of protein and fat, whereas animals grazing only on pasture had a more favorable fatty acid profile and water holding capacity. In conclusion, the supplementation of concentrate at a rate of about 1.5% of body weight is recommended to improve the performance and carcass quality of buffaloes.

Isolation and Genetic Transformation of Primordial Germ Cell (PGC)-Derived Cells from Cattle, Goats, Rabbits and Rats

Lee, C.K.,Moore, K.,Scales, N.,Westhusin, M.,Newton, G.,Im, K.S.,Piedrahita, J.A. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.5

At present embryonic stem (ES) cells with confirmed pluripotential properties are only available in the mouse. Recently, we were able to isolate, culture and genetically transform primordial germ cell (PGC)-derived cells from pig embryos and demonstrate their ability to contribute to chimera development in the pig. In order to determine whether the system we developed could be used to isolate embryonic germ (EG) cells from other mammalian species, we placed isolated PGCs from cattle, goats, rabbits and rats in culture. Briefly, PGCs were isolated from fetuses of cow (day 30-50), goat (day 25), rabbit (day 15-18) and rat (day 11-12), and plated on STO feeder cells in Dulbecco's modified Eagle's medium (DMEM): Ham's F10 medium (1:1) supplemented with 0.01 mM nonessential amino acids, 2 mM L-glutamine, 0.1 mM $\beta$ - mercaptoethnol, soluble recombinant human stem cell factor (SCF; 40ng/ml), human basic fibroblast growth factor (bFGF; 20ng/ml) and human leukemia inhibitory factor (LIF; 20ng/ml). For maintenance of the cells, colonies were passed to fresh feeders every 7-10 days. In all species tested, we were able to obtain and maintain colonies with ES-like morphology. Their developmental potential was tested by alkaline phosphatase (AP) staining and in vitro differentiation assay. For genetic transformation, cells were electroporated with a construct containing the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. GFP-expressing colonies were detected in cattle, rabbits and rats. These results suggest that PGC-derived cells from cattle, goats, rabbits and rats can be isolated, cultured, and genetically transformed, and provide the basis for analyzing their developmental potential and their possible use for the precise genetic modification of these species.

Assessment of Bradykinesia, Akinesia and Rigidity Using a Home-based Assessment Tool

L. Cunningham,C. Nugent,G. Moore,D. Finlay,D. Craig 동국대학교 정보융합기술원 2009 International Journal of Assistive Robotics and Sy Vol.10 No.4

An increase in the prevalence of age related diseases such as Parkinson's Disease (PD) and the increasingly aging population has created a need for appropriate health and social care services for the elderly and disabled. PD requires close monitoring and regular assessment. In order to be assessed and monitored, people are usually required to attend a clinic or hospital. In this study a home-based assessment tool, which collects information on people’s hand and finger movements, has been developed and evaluated. The tool collects data on the time taken to make a series of movements, the speed at which the movements are made and the path that is taken for each movement. Through the use of this tool movement difficulties such as bradykinesia, akinesia and rigidity could be identified within the home environment. Such an approach has the potential to decrease the number of clinic/hospital visits a person with PD requires. It could also assist with making the current, somewhat subjective, methods of PD assessment become more objective. If used over a long period of time, for example, daily or weekly, it could help identify how effective or otherwise a person's medication is. This would be achieved by the tool collecting data on a regular basis in order to establish what is considered to be ‘normal’ results for that particular person. So for example, a pattern may emerge over a few months that each time a particular participant is ‘off’ they take the same or similar length of time to use the tool. It could be suggested that the length of time the participant took to use the tool when ‘off’ during those months was their ‘normal’ result for their ‘off’ state. If, after a period of time, the results collected seemed ‘abnormal’ for that person it could indicate that a visit to the clinician is needed to identify if the medication needs changed. The developed tool was evaluated by twenty participants. Half of the participants had been diagnosed with PD and the other half were a control group without the disease. Within each group there were a mix of participants who were computer literate and those who had no experience of using computers. Results following evaluation of the tool indicated that the control group were able to conduct assessments using the tool in a time that was significantly shorter than that taken by the PD group (p = 0.034). This in turn suggests that such tools have the ability to assist with enabling more objective assessment of PD.

Assessment of Bradykinesia, Akinesia and Rigidity Using a Home-based Assessment Tool

L,Cunningham,C,Nugent,G,Moore,D,Finlay,D,Craig 동국대학교 정보융합기술원 2009 International Journal of Assistive Robotics and Sy Vol.10 No.4

An increase in the prevalence of age related diseases such as Parkinson's Disease (PD) and the increasingly aging population has created a need for appropriate health and social care services for the elderly and disabled. PD requires close monitoring and regular assessment. In order to be assessed and monitored, people are usually required to attend a clinic or hospital. In this study a home-based assessment tool, which collects information on people’s hand and finger movements, has been developed and evaluated. The tool collects data on the time taken to make a series of movements, the speed at which the movements are made and the path that is taken for each movement. Through the use of this tool movement difficulties such as bradykinesia, akinesia and rigidity could be identified within the home environment. Such an approach has the potential to decrease the number of clinic/hospital visits a person with PD requires. It could also assist with making the current, somewhat subjective, methods of PD assessment become more objective. If used over a long period of time, for example, daily or weekly, it could help identify how effective or otherwise a person's medication is. This would be achieved by the tool collecting data on a regular basis in order to establish what is considered to be ‘normal’ results for that particular person. So for example, a pattern may emerge over a few months that each time a particular participant is ‘off’ they take the same or similar length of time to use the tool. It could be suggested that the length of time the participant took to use the tool when ‘off’ during those months was their ‘normal’ result for their ‘off’ state. If, after a period of time, the results collected seemed ‘abnormal’ for that person it could indicate that a visit to the clinician is needed to identify if the medication needs changed. The developed tool was evaluated by twenty participants. Half of the participants had been diagnosed with PD and the other half were a control group without the disease. Within each group there were a mix of participants who were computer literate and those who had no experience of using computers. Results following evaluation of the tool indicated that the control group were able to conduct assessments using the tool in a time that was significantly shorter than that taken by the PD group (p = 0.034). This in turn suggests that such tools have the ability to assist with enabling more objective assessment of PD.

Financial Liberalization, Market Discipline And Bank Risk

( Ja Hyeong Koo ),( William C. Gruben ),( Robert R. Moore ) 한국금융연구원 2005 기타보고서 Vol.2005 No.1

Determination of the Survival of Bacteriophage M13 from Chemical and Physical Challenges to Assist in Its Sustainable Bioprocessing

Steven D. Branston,Emma C. Stanley,John M. Ward,Eli Keshavarz-Moore 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3

Bacteriophages are naturally infectious particles that replicate extremely efficiently in their bacterial hosts. Consequently, a facility processing bioproducts from a bacterial strain would be typically expected to focus on avoiding bacteriophage contamination. However, bacteriophages themselves are now showing great promise as a whole new class of industrial agents, such as biologically based nano-materials, delivery vectors and antimicrobials. This therefore raises a new challenge for their large-scale manufacture, potentially in contracted facilities shared with the host organism. The key issue is that knowledge of individual bacteriophage behaviour in the face of physical and chemical challenges is frequently incomplete, complicating decision-making regarding their safe introduction to a facility. This study tackles this issue for the filamentous bacteriophage M13. It was found that experimentation to determine an effective decontamination agent was important:Two of the three tested were ineffective. Virkon was considered to be the disinfectant of choice. Bacteriophage M13 was confirmed to be highly desiccation resistant,exhibiting a half-life of up to 120 days. Conversely, it was completely inactivated by strongly acidic and alkaline conditions and by temperatures above 95oC. By understanding the response of a bacteriophage to these challenges, steps towards their sustainable manufacture can be achieved.