Prognostic impact of reduced tumor-free margin distance on long-term survival in FIGO stage IB/II vulvar squamous cell carcinoma

Leonardo Micheletti,Mario Preti,Viviana Cintolesi,Elisabetta Corvetto,Silvana Privitera,Eleonora Palmese,Chiara Benedetto 대한부인종양학회 2018 Journal of Gynecologic Oncology Vol.29 No.5

Objective: We aimed to identify the minimum tumor-free margin distance conferring long-term oncological safety in patients diagnosed with International Federation of Gynecology and Obstetrics (FIGO) stage IB/II vulvar squamous cell carcinoma (VSCC). Methods: This was a retrospective cohort study in patients with stage IB/II VSCC treated at a single institution in Turin, Italy. The main aim was to identify the minimum tumor-free margin distance that confers oncological safety in early-stage VSCC. Patients were divided in groups according to tumor-free histological margin distance to compare survival outcomes. Overall survival (OS), disease-specific survival (DSS), and recurrence rate (RR) were estimated by the Kaplan-Meier method for the newly proposed and the currently recommended 8 mm margin cut-off. Log-rank test was used to compare survival between groups. Results: One hundred and fourteen patients met the study criteria. Median age was 68 years and median follow-up was 80 months. The minimum margin distance that conferred long-term oncological safety was 5 mm. OS, DSS were significantly lower in the <5 mm group when compared with the ≥5 mm group (p=0.002 and p=0.033, respectively) although no difference in RR was observed between groups. Analysis at the 8-mm cut-off indicated there is no difference in OS, DSS, or RR between groups. Conclusion: FIGO stage IB/II VSCC patients' prognosis is affected by margin distance. Long-term survival is significantly reduced in patients with tumor-free margins <5 mm, even in the absence of lymph node metastasis. Thus, these patients should be offered further surgical or adjuvant treatment.

An analytical benchmark and a <i>Mathematica</i> program for MD codes: Testing LAMMPS on the 2nd generation Brenner potential

Favata, Antonino,Micheletti, Andrea,Ryu, Seunghwa,Pugno, Nicola M. Elsevier 2016 Computer physics communications Vol.207 No.-

<P><B>Abstract</B></P> <P>An analytical benchmark and a simple consistent <I>Mathematica</I> program are proposed for graphene and carbon nanotubes, that may serve to test any molecular dynamics code implemented with REBO potentials. By exploiting the benchmark, we checked results produced by LAMMPS (Large-scale Atomic/Molecular Massively Parallel Simulator) when adopting the second generation Brenner potential, we made evident that this code in its current implementation produces results which are offset from those of the benchmark by a significant amount, and provide evidence of the reason.</P> <P><B>Program summary</B></P> <P> <I>Program title:</I> MDBenchmarks</P> <P> <I>Catalogue identifier:</I> AFAS_v1_0</P> <P> <I>Program summary URL:</I> http://cpc.cs.qub.ac.uk/summaries/AFAS_v1_0.html </P> <P> <I>Program obtainable from:</I> CPC Program Library, Queen’s University, Belfast, N. Ireland</P> <P> <I>Licensing provisions:</I> GNU GPL v3</P> <P> <I>No. of lines in distributed program, including test data, etc.:</I> 22854</P> <P> <I>No. of bytes in distributed program, including test data, etc.:</I> 369171</P> <P> <I>Distribution format:</I> tar.gz</P> <P> <I>Programming language:</I> Mathematica 9.</P> <P> <I>Computer:</I> Any PC.</P> <P> <I>Operating system:</I> Any which supports Mathematica; tested under OS Yosemite.</P> <P> <I>RAM:</I> < 5 gigabytes</P> <P> <I>Classification:</I> 7.7, 16.1, 16.13.</P> <P> <I>Nature of problem:</I> Testing commercial or open-source molecular dynamics codes implementing off-the-shelf REBO potentials on an analytical benchmark.</P> <P> <I>Solution method:</I> Analytical equilibrium conditions for achiral carbon nanotubes are implemented and solved, delivering benchmark values for the corresponding natural radius and cohesive energy; material properties (Young’s modulus and Poisson coefficient) are also computed.</P> <P> <I>Running time:</I> Instantaneous, or a few seconds, depending on computer hardware</P>

Towards modular bone tissue engineering using Ti–Co-doped phosphate glass microspheres: cytocompatibility and dynamic culture studies

Peticone, Carlotta,De Silva Thompson, David,Owens, Gareth J,Kim, Hae-Won,Micheletti, Martina,Knowles, Jonathan C,Wall, Ivan SAGE Publications 2017 Journal of biomaterials applications Vol.32 No.3

Risk factors for unrecognized invasive carcinoma in patients with vulvar high­grade squamous intraepithelial lesion at vulvoscopy­directed biopsy

Mario Preti,Lauro Bucchi,Bruno Ghiringhello,Silvana Privitera,Valentina Frau,Elisabetta Corvetto,Chiara Benedetto,Leonardo Micheletti 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.4

Objective: To evaluate the prevalence and risk factors for unrecognized invasive carcinomain a series of patients undergoing surgical excision after an office biopsy of vulvar high-gradesquamous intraepithelial lesion (VHSIL). Methods: Two hundred and sixteen consecutive patients treated in a tertiary-level referralcenter for vulvar disease in north-western Italy were recruited. Patients' records werereviewed by trained personnel. Factors showing a statistically significant (p<0.05) associationwith detection of stromal invasion at excisional surgery in univariate analysis were furtherexamined in a backward stepwise multiple logistic regression model. Results: The median patient age was 50 years (range, 19–88). More than 25% patients withVHSIL at biopsy had associated cervical/vaginal intraepithelial neoplasia, and more than 35%had a multifocal lesion. Invasive carcinoma was detected in surgical specimens from 24 patients(11%). The depth of stromal invasion varied between 0.1 mm and 3.0 mm with a median of 0.5mm. In multivariate analysis, the risk of invasive carcinoma detection was greater for patients inthe highest tertile of age (p=0.008), for patients with a lesion ≥20 mm in size (p=0.013) and withclitoral involvement (p<0.001), and for patients presenting with a nodular lesion (p=0.078). Conclusion: Our study suggests that patient age, lesion size, clitoral involvement andnodular appearance in patients with VHSIL at vulvoscopy-directed biopsy are independentlyassociated with the risk of unrecognized invasive carcinoma.

Effects of Chrysin on Urinary Testosterone Levels in Human Males

Cristiana Gambelunghe,Ruggero Rossi,Marco Sommavilla,Chiara Ferranti,Riccardo Rossi,Chiara Ciculi,Stefania Gizzi,Alessandra Micheletti,Stefano Rufini 한국식품영양과학회 2003 Journal of medicinal food Vol.6 No.4

The equilibrium of sexual hormones in both sexes is controlled in vertebrates by the enzyme aromatase, amember of the cytochrome P450 superfamily, which catalyzes the conversion of androstenedione and testosterone into es-trone and estradiol, respectively. Flavonoids are diphenolic compounds present in whole grains, legumes, fruits, and vegeta-bles that are strongly implicated as protective in coronary heart disease, stroke, and cancer. One flavonoid, chrysin, found inhigh concentrations in honey and propolis, has been shown to be an inhibitor of aromatase enzyme activity. These foods areoften used as supplements, particulary by sportsmen for their energetic and antioxidant properties. The aim of this study wasto verify if daily treatment for 21 days with propolis and honey, containing chrysin, would modify urinary concentrations oftestosterone in volunteer male subjects. In fact, aromatase inhibition by chrysin could block the conversion of androgens intoestrogens with a consequent increase of testosterone, eventually measurable in urine samples. The obtained data did not showalterations of the levels of testosterone in the volunteers after 7, 14, and 21 days of treatment in comparison with baselinevalues and compared with measurements on the control subjects at the same time. In conclusion, the use of these foods for21 days at the doses usually taken as oral supplementation does not have effects on the equilibrium of testosterone in humanmales.

Radial access for percutaneous coronary procedure: relationship between operator expertise and complications

Simona Susanu,Marco Angelillis,Cristina Giannini,Rossella Binella,Anna Matteoni,Rita Bellucci,Sandro Balestri,Nicola Ferrara,Federico Falchi,Giuliano Micheletti,Anna Sonia Petronio 대한응급의학회 2018 Clinical and Experimental Emergency Medicine Vol.5 No.2

Objective The aim of this study was to investigate (1) whether the learning curve of new catheterization laboratory operators increases the incidence of complications of transradial access during percutaneous coronary interventions and (2) whether manual compression with a two-step approach is safe and efficient for radial access hemostasis. Methods We performed a prospective study with all consecutive patients who underwent a coronary diagnostic or intervention procedure with radial access. The primary end point was a composite of pulseless radial artery of the wrist and hematoma evaluated after 24 hours. The secondary end point of efficacy was defined as the presence of bleeding or hematoma after 30 seconds. Results From March 2016 to June 2016, 150 consecutive patients, of whom 147 underwent coronary angiography and/or percutaneous coronary intervention through radial access, were included in the present study. The primary end point was present in 33%, but pulseless radial artery of the wrist was present only in 5.3%. We found that the incidence of primary end point was statistically different according to the number of puncture attempts, with a cutoff of two punctures with blood. The secondary end point of safety was present only in 4.7% of the cases. Conclusion Radial access is feasible and safe even if performed by training physicians. Manual compression with early evaluation after 30 seconds is a safe technique for managing the radial access after sheath removal.