Spine Surgical Robotics: Current Status and Recent Clinical Applications

Jiangtao Wang,Junxian Miao,Yi Zhan,Yongchao Duan,Yuanshun Wang,Dingjun Hao,Biao Wang 대한척추신경외과학회 2023 Neurospine Vol.20 No.4

With the development of artificial intelligence and the further deepening of medical-engineering integration, spine surgical robot-assisted (RA) technique has made significant progress and its applicability in clinical practice is constantly expanding in recent years. In this review, we have systematically summarized the majority of literature related to spine surgical robots in the past decade, and not only classified robots accordingly, but also summarized the latest research progress in RA technique for screw placement such as cervical, thoracic, and lumbar pedicle screws, cortical bone trajectory screws, cervical lateral mass screws, and S2 sacroiliac screws; guiding targeted puncture and placement of endoscope via the intervertebral foramen; complete resection of spinal tumor tissue; and decompressive laminectomy. In addition, this report also provides a detailed evaluation of RA technique’s advantages and disadvantages, and clarifies the accuracy, safety, and practicality of RA technique. We consider that this review can help clinical physicians further understand and familiarize the current clinical application status of spine surgical robots, thereby promoting the continuous improvement and popularization of RA technique, and ultimately benefiting numerous patients.

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Wang, Lei,Wang, Qiu-Tong,Liu, Yu-Peng,Dong, Qing-Qing,Hu, Hai-Jie,Miao, Zhi,Li, Shuang,Liu, Yong,Zhou, Hao,Zhang, Tong-Cun,Ma, Wen-Jian,Luo, Xue-Gang The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

Biochemical Characterization of a Psychrophilic Phytase from an Artificially Cultivable Morel Morchella importuna

( Hao Tan ),( Jie Tang ),( Xiaolin Li ),( Tianhai Liu ),( Renyun Miao ),( Zhongqian Huang ),( Yong Wang ),( Bingcheng Gan ),( Weihong Peng ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.12

Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of the histidine acid phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified, and characterized. The phytase had an optimum temperature at 25°C, which is the lowest among all the known phytases to our best knowledge. The optimum pH (6.5) is higher than most of the known HAP phytases, which is fit for the weak acidic condition in fish gut. At the optimum pH and temperature, MiPhyA showed the maximum activity level (2,384.6 ± 90.4 μmol·min^-1·mg^-1, suggesting that the enzyme possesses a higher activity level over many known phytases at low temperatures. The phytate-degrading efficacy was tested on three common feed materials (soybean meal/rapeseed meal/corn meal) and was compared with the well-known phytases of Escherichia coli and A. niger. When using the same amount of activity units, MiPhyA could yield at least 3× more inorganic phosphate than the two reference phytases. When using the same weight of protein, MiPhyA could yield at least 5× more inorganic phosphate than the other two. Since it could degrade phytate in feed materials efficiently under low temperature and weak acidic conditions, which are common for aquacultural application, MiPhyA might be a promising candidate as a feed additive enzyme.

Risk Factors for Recurrent Colorectal Polyps

Yuanzhen Hao,Yining Wang,Miao Qi,Xin He,Ying Zhu,Junbo Hong 거트앤리버 소화기연관학회협의회 2020 Gut and Liver Vol.14 No.4

The recurrence of colorectal polyps is caused by various factors and leads to the carcinogenesis of colorectal cancer, which ranks third in incidence and fourth in mortality among cancers worldwide. The potential risk factors for colorectal polyp recurrence have been demonstrated in multiple trials. However, an article that pools and summarizes the various results is needed. This review enumerates and analyzes some risk factors in terms of patient characteristics, procedural operations, polyp characteristics, and dietary aspects to propose some effective prophylactic measures. This review aimed to provide a reference for clinical application and guide patients to prevent colorectal polyp recurrence in a more effective manner.

Enhanced Photoelectrochemical Water Splitting and Photocatalytic Water Oxidation of Cu2O Nanocube-Loaded BiVO4 Nanocrystal Heterostructures

Wenzhong Wang,Weiwei Zhang,Shan Meng,Lujie Jia,Miao Tan,Chenchun Hao,Yujie Liang,JUN WANG,Bin Zou 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.6

Reducing the fast recombination of photogenerated electron-hole pairsof semiconductor photocatalyst is very important to improve itsphotocatalysis. In this paper we fabricate Cu2O nanocube-decoratedBiVO4 nanocrystal (denoted as BiVO4@Cu2O nanocrystal@nanocube)heterostructure photocatalyst by coupling n-type BiVO4 with p-type Cu2O. The BiVO4@Cu2O nanocrystal@nanocube photocatalysts show superiorphotocatalytic activities in photoelectrochemical (PEC) activity andphotocatalytic water oxidation to BiVO4 photocatalysts under visible lightillumination. The BiVO4@Cu2O nanocrystal@nanocube heterostructureelectrode achieves the highest photocurrent density of ~ 10 μA cm−2 at 0 Vversus Ag/AgCl, 5 times higher than that of BiVO4 nanocrystal electrode (~ 2 μA cm−2). The light induced evolution rate of O2generation for BiVO4@Cu2O nanocrystal@nanocube heterostructures is as high as 150 μmol h−1100 mg cat−1, more than 3times higher than that (48 μmol h−1100 mg cat−1) of BiVO4 nanocrystals. The enhanced photocatalysis activities of theBiVO4@Cu2O nanocrystal@nanocube photocatalysts are attributed to the efficient separation of the photoexcited electron-holepairs caused by inner electronic field (IEF) of p-n junction. This study opens up new opportunities in designing photoactivematerials with highly enhanced performance for solar energy conversion.

Apoptosis Induction Effects of EGCG in Laryngeal Squamous Cell Carcinoma Cells through Telomerase Repression

Xi Wang,Miao-Wang Hao,Ke Dong,Fang Lin,Ji-Hong Ren,Hui-Zhong Zhang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.9

Epigallocatechin-3-gallate (EGCG), the major component of green tea polyphenol, has potent efficiency to prevent the growth of a variety of cancer cells. As a novel anticancer agent for treatment of cancers, EGCG is promising and the mechanism has not been fully understood. Laryngeal squamous cell carcinoma (LSCC) is one common tumor in head and neck cancers. In the present study, we assess the effects of EGCG on LSCC cell line Hep-2, and their possible involvement in EGCG-induced apoptosis. The result showed that treatment of Hep-2 cells with EGCG decreased the cell viability, inhibited the growth and proliferation, induced apoptosis and increased the activity of caspase-3 in a dose-dependent manner. Furthermore, we found that EGCG-treatment repressed telomerase activity effectively in a concentrationdependent manner. The combined results show that EGCG induced apoptosis in Hep-2 cells via inhibiting the telomerase activity.

Absence of EZH2 Gene Mutation in Chronic Myeloid Leukemia Patients in Blast Crisis

Chen, Hao-Yue,Yao, Hong,Wu, Ling-Yu,Liu, Can-Jun,Zhu, Jian-Qin,Liu, Chun-Hua,Wang, Wei,Dong, Sha-Sha,Ping, Na-Na,Chen, Su-Ning,Sun, Miao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Lei Wang,Qiu-Tong Wang,Yu-Peng Liu,Qing-Qing Dong,Hai-Jie Hu,Zhi Miao,Shuang Li,Yong Liu,Hao Zhou,Tong-Cun Zhang,Wen-Jian Ma,Xuegang Luo 대한위암학회 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

Thermo-optic Characteristics of Micro-structured Optical Fiber Infiltrated with Mixture Liquids

Ran Wang,Yuye Wang,Yinping Miao,Ying Lu,Nannan Luan,Congjing Hao,Liangcheng Duan,Cai Yuan,Jianquan Yao 한국광학회 2013 Current Optics and Photonics Vol.17 No.3

We present both theoretically and experimentally the thermo-optic characteristics of micro-structured optical fiber (MOF) filled with mixed liquid. The performance of MOF depends on the efficient interaction between the fundamental mode of the transmitted light wave and the tunable thermo-optic materials in the cladding. The numerical simulation indicates that the confinement loss of MOF presents higher temperature dependence with higher air-filling ratios d/Λ, longer incident wavelength and fewer air holes in the cladding. For the 4cm liquid-filled grapefruit MOF, we demonstrate from experiments that different proportions of solutions lead to tunable temperature sensitive ranges. The insertion loss and the extinction ratio are 3~4 dB and approximate 20 dB, respectively. The proposed liquid-filling MOF will be developed as thermo-optic sensor, attenuator or optical switch with the advantages of simple structure, compact configuration and easy fabrication.

CDN Scalability Improvement using a Moderate Peer-assisted Method

( Peichang Shi ),( Huaimin Wang ),( Hao Yin ),( Bo Ding ),( Tianzuo Wang ),( Miao Wang ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.3

Content Delivery Networks (CDN) server loads that fluctuant necessitate CDN to improve its service scalability especially when the peak load exceeds its service capacity. The peer assisted scheme is widely used in improving CDN scalability. However, CDN operators do not want to lose profit by overusing it, which may lead to the CDN resource utilization reduced. Therefore, improving CDN scalability moderately and guarantying CDN resource utilization maximized is necessary. However, when and how to use the peer-assisted scheme to achieve such improvement remains a great challenge. In this paper, we propose a new method called Dynamic Moderate Peer-assisted Method (DMPM), which uses time series analysis to predict and decide when and how many server loads needs to offload. A novel peer-assisted mechanism based on the prediction designed, which can maximize the profit of the CDN operators without influencing scalability. Extensive evaluations based on an actual CDN load traces have shown the effectiveness of DMPM.