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비효소적 핵산 분해 반응 : DNA에 대한 몇가지 Organometallointercalator Some Organometallointercalator for DNA
고동성,박미경,류형원,서일환 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-
Cu^(2+) -nalidixate 또는 Cu^(2+) -pipemidate 시스템을 H_2O_2 및 아스코르빈산 존재하 방사능-표지된 DNA와 함께 37℃에서 배양시키므로써 산-용해성 분해산물들이 생성되었다. 본성 DNA 대신 변성 DNA를 기질로 쓸 경우 금속-drug 시스템의 DNA 절단활성은 현저하게 감소되었다. 또한 본성 DNA에 대한 절단활성은 DNA에의 전형적 intercalator이며 metalloquinolones의 DNA에의 결합에 대한 경쟁적 방해제가 될 수 있는 ethidium bromide에 의하여 저하되었다. Quinolone 항생제가 금속이온과의 킬레이트 복합체 형성을 통하여 DNA의 두가닥 나사선 염기쌍 사이에의 삽간결합(intercalation)을 할 수 있으며 이와 같은 결합을 이룬 금속복합체는 DNA 가닥에 대한 산화성 절단활성을 가질 수 있음을 시사한다. Incubation of Cu^(2+)-nalidixate or Cu^(2+)-pipemidate system with DNA at 37℃ in the presence of H_2O_2 and ascorbic acid resulted in the production of acid-soluble counts from dadioactively labeled DNA. With denatured DNA as substrate, the cleavage activity of the metalloquinolone systems was far less than with the native DNA. The DNA cleavage activity of metalloquinolones was also decreased by the addition of ethidium bromide, which was known to be a typical intercalator of DNA and was shown to be a competitive inhibitor for the metalloquinolone binding to DNA. The data here suggest the DNA cleavage activity of quinolone antibiotics intercalated into DNA as metal chelate complexes.
12주간의 유산소성 운동이 비만 초등학생의 신체조성과 혈액성분에 미치는 영향
윤미수,최건식,고성경,정소봉 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5
[Purpose] The purpose of this study was to investigate the effect of aerobic exercise training on the body composition and blood components in obese elementary children after 12 weeks. [Methods] Subjects of this study were composed of 20 male students ranging in age from 9 to 12 years who were classified into two groups, obese exercise group(n=10) and normal control group(n=10). For 12 weeks, obese exercise group performed the aerobic exercise training that the program was composed by Yoon. The blood samples were taken before and after 12 week Paired t-Test was adopted to analyse the difference between before and after aerobic exercise training. [Results] body fat of obese exercise group was decreased 4.8% after aerobic exercise training(p<.01) and that of normal control group was decreased 0.1% during same time(NS). Among the blood lipoprotein, total cholesterol(p<.01), LCL-C(p<.01), and triglycerides(p<.05) of obese exercise group were decreased after aerobic exercise training, but not changed HDL-C of same group. Red blood cell, Hemoglobin(p<.65) of obese exercise group were increased after aerobic exercise training. Glucose of obese exercise group were decreased after aerobic exercise training(p<.05), but not changed in Ca and P. [Conclusion] In conclusion, aerobic exercise training for 12 weeks seems to reduce body might and body fat, and to change the profiles of blood Component in obese elementary children.
노치권(외 6명) 신라대학교 자연과학연구소 2012 自然科學論文集 Vol.22 No.-
To study fauna of Silla University located in Mt. Baek-yang, we conducted six surveys from 29 May 2011 to 28 April 2012. We found five phyla 41 orders 197 species of animals inhabiting in this area: four phyla 27 orders 163 species of invertebrates and in vertebrates one order six species of fishes, three orders nine species of amphibians and reptiles, six orders 14 species of aves, and four orders five species of mammals. Of these species, some Korean endemic species were included: a fish of Rhodeus uyekii in a pond near main gate of this university, a frog of Rana coreana in a creek near a dormitory, and a rabbit of Lepus coreanus and a water deer of Hydropotes inermis in a grass region near a main stadium. A kind of hawk, Falco tinnunculus as a natural monument (animal) of no. 323-8, was observed on a building of medical and life science. A blue frog of Hyla japonica as a variation was found near a spring resort. Therefore, this area is considered an ecologically important area having a high animal diversity.
Mathematically Gifted Students` Analogy in Statistics
( Mi Mi Park ),( Eun Sung Ko ),( Dong Hwan Lee ),( Kyeong Hwa Lee ) 한국수학교육학회 2012 수학교육 학술지 Vol.2012 No.2
Statistical thinking handles concrete data. However, many of the concepts used in statistics are abstract in nature. In statistics, analogy can be used and should be used to grasp and to represent abstract concepts. Statistics is regarded as different discipline from mathematics due to various aspects of statistics, especially the crucial role of context. This study investigated mathematically gifted students` analogy in statistics. The gifted were asked to construct similar problems to base problem that is a statistical problem. On the basis of analysis on students` new problems, researchers could classify them into four types of analogy in statistics: Success in both structural and contextual analogy, success in structural analogy, structural generalization, and failure in both structural and contextual analogy.
고미경 ( Ko Mi-kyeong ),배명훈 ( Bae Myeong-hun ),김영식 ( Kim Young-sik ) 국제문화&예술학회 2023 국제문화예술 Vol.4 No.2
연구목적: 본 연구는 초등학교 교사의 놀이체육수업이 어떻게 전개되고 있는지 심도 있게 살펴보고, 놀이체육에 대한 교사들의 인식은 무엇인지 알아보고자 하였다. 연구방법: 이 연구를 위해 초등학교에서 놀이체육을 활용하고 있는 현직 교사 7명을 연구 참여자로 선정하여 질적 연구를 실시하였다. 본 연구의 주된 자료 수집은 심층면담을 통해 이루어졌으며 연구 참여자별로 2∼4회에 걸쳐 실시되었다. 또한 수업 지도안, 수업 일지, 학생 편지 등의 문서 수집을 통해 면담 자료의 구체성을 더하였다. 연구결과: 초등교사의 놀이체육 활용 유형은 사전 준비 없이도 쉽게 할 수 있는 체육수업으로써의 ‘즉흥적 수업’, 학생들의 흥미를 우선으로 하여 내용을 선정하는 ‘유희 중심 수업’, 학급 운영 및 수업 관리 계획의 한 방법으로 활용하는 ‘보상용 수업’ 세가지 유형으로 분석되었다. 둘째, 놀이체육수업에 대한 초등교사의 인식은 ‘초등체육의 새 흐름’과 ‘또 다른 아나공 수업’으로 나타났다. 결론: 본 연구는 놀이 중심으로 운영되는 초등학교 체육수업 모습을 생생하게 제공함으로써 초등학교 체육수업이 직면한 상황을 구체적으로 드러내고 초등체육의 발전을 위한 시사점을 제공한다는 점에서 의의가 있다. Purpose: This study was intended to examine in depth how elementary school teachers’ drama physical education classes are developing, and to find out how teachers’ perceptions of play physical education are. Methods: Qualitative study was conducted by selecting 8 incumbent teachers who are using play sports in elementary school as research participants. The main data collection in this study was conducted through in-depth interviews, which were conducted 2 to 4 times for each research participant. In addition, the specificity of the interview data was added by collecting documents such as class guidance plans, class diaries, and student letters. Result: First, the use of play sports by elementary school teachers is ‘improvisation class’, which is a physical education class that can be easily performed without prior preparation, ‘play-centered class’, which prioritizes students’ interest in content selection, class operation and class management plan. Second, elementary school teachers’ perceptions of play physical education classes appeared as ‘a new trend in elementary physical education’ and ‘another anagong class’. Elementary school teachers recognize play sports as an alternative in the midst of a vacuum in educational content, reduced opportunities for physical education classes, and the demands of the play-centered era. In addition, on the one hand, it was analyzed that the critical perception that play physical education classes foster students who think of physical education as play and teachers who do not study physical education is mixed. Conclusion: This study is meaningful in that it provides vividly the appearance of elementary school physical education classes operated centering on play, thereby revealing the situation faced by elementary physical education classes in detail and providing implications for the development of elementary physical education.
Evaluation of improved vaccine for type C Foot-and-Mouth Disease in cattle and pigs
Su-Hwa You(Su-Hwa You),Hye-Eun Jo(Hye-Eun Jo),Joo-Hyung Choi(Joo-Hyung Choi),Mi-Kyeong Ko(Mi-Kyeong Ko),Sung Ho Shin(Sung Ho Shin),Min Ja Lee(Min Ja Lee),Su-Mi Kim(Su-Mi Kim),Byounghan Kim(Byounghan K 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-
Evaluation of vaccines for the SAT 1, SAT 2, and SAT 3 serotypes of Foot-and-Mouth Disease in pigs
Hye-Eun Jo(Hye-Eun Jo),Su-Hwa You(Su-Hwa You),Joo-Hyung Choi(Joo-Hyung Choi),Mi-Kyeong Ko(Mi-Kyeong Ko),Sung Ho Shin(Sung Ho Shin),Hyundong Jo(Hyundong Jo),Min Ja Lee(Min Ja Lee),Su-Mi Kim(Su-Mi Kim) 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-