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Sankararaman Hariharakrishnan,Manickavasagam Sagadai,Triapitsyn Serguei Vladimirovich 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1
Four new species of Anagrus (Anagrus) Haliday (Hymenoptera: Mymaridae) are described from India: A. (Anagrus) karnatakus Triapitsyn sp. n., A. (Anagrus) kolhapurensis Manickavasagam & Sankararaman sp. n., A. (Anagrus) latus Manickavasagam & Sankararaman sp. n. and A. (Anagrus) sujathae Manickavasagam & Sankar araman sp. n. A key to females of the 15 described and one undescribed species of Anagrus known from India is provided. www.zoobank.org/urn:lsid:zoobank.org:pub:99ED388D-BE3E-4FBA-A383-3482D96DA42F.
Drug- and Gene-eluting Stents for Preventing Coronary Restenosis
Kamali Manickavasagam Lekshmi,Hui-Lian Che,조정수,박인규 전남대학교 의과학연구소 2017 전남의대학술지 Vol.53 No.1
Coronary artery disease (CAD) has been reported to be a major cause of death worldwide. Current treatment methods include atherectomy, coronary angioplasty (as a percutaneous coronary intervention), and coronary artery bypass. Among them, the insertion of stents into the coronary artery is one of the commonly used methods for CAD, although the formation of in-stent restenosis (ISR) is a major drawback, demanding improvement in stent technology. Stents can be improved using the delivery of DNA, siRNA, and miRNA rather than anti-inflammatory/anti-thrombotic drugs. In particular, genes that could interfere with the development of plaque around infected regions are conjugated on the stent surface to inhibit neointimal formation. Despite their potential benefits, it is necessary to explore the various properties of gene-eluting stents. Furthermore, multifunctional electronic stents that can be used as a biosensor and deliver drug- or gene-based on physiological condition will be a very promising way to the successful treatment of ISR. In this review, we have discussed the molecular mechanism of restenosis, the use of drug- and gene-eluting stents, and the possible roles that these stents have in the prevention and treatment of coronary restenosis. Further, we have explained how multifunctional electronic stents could be used as a biosensor and deliver drugs based on physiological conditions
Drug- and Gene-eluting Stents for Preventing Coronary Restenosis
Lekshmi, Kamali Manickavasagam,Che, Hui-Lian,Cho, Chong-Su,Park, In-Kyu Chonnam National University Medical School 2017 CMJ Vol.53 No.1
<P>Coronary artery disease (CAD) has been reported to be a major cause of death worldwide. Current treatment methods include atherectomy, coronary angioplasty (as a percutaneous coronary intervention), and coronary artery bypass. Among them, the insertion of stents into the coronary artery is one of the commonly used methods for CAD, although the formation of in-stent restenosis (ISR) is a major drawback, demanding improvement in stent technology. Stents can be improved using the delivery of DNA, siRNA, and miRNA rather than anti-inflammatory/anti-thrombotic drugs. In particular, genes that could interfere with the development of plaque around infected regions are conjugated on the stent surface to inhibit neointimal formation. Despite their potential benefits, it is necessary to explore the various properties of gene-eluting stents. Furthermore, multifunctional electronic stents that can be used as a biosensor and deliver drug- or gene-based on physiological condition will be a very promising way to the successful treatment of ISR. In this review, we have discussed the molecular mechanism of restenosis, the use of drug- and gene-eluting stents, and the possible roles that these stents have in the prevention and treatment of coronary restenosis. Further, we have explained how multifunctional electronic stents could be used as a biosensor and deliver drugs based on physiological conditions.</P>
Selvam Sathish,Venkatachalam Vasudevan,Sivabalan Karthik,Gadamchetty Pavan,Markandan Manickavasagam 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.6
Hybanthus enneaspermus is an important source for L-Dopa production. This study reports elicited L-Dopa production in cell suspension cultures of Hybanthus enneaspermus. Cell suspension culture was established using green friable calli from leaf explants cultured on MS medium containing 2.0 mg/l 2,4-D. Effects of different elicitors such as SA, YE, MeJA and AgNO3 on biomass accumulation and L-Dopa content were studied. Among the elicitor tested SA treated culture produced highest biomass and L-Dopa according to their exposure time and concentration. Maximum biomass of 15.5 ± 0.16 g FW, 4.05 ± 0.18 g DW and L-Dopa production of 8.88 mg/g DW were observed at 150 μM concentration of SA. This was 9.25-fold higher compared to that of the unelicited control culture. The results obtained in this study clearly show that the elicita-tion strategy is a promising method for biosynthesis of L-Dopa production by cell suspension cultures of H. enneaspermus.
Seok, Hae-Yong,Sanoj Rejinold, N.,Lekshmi, Kamali Manickavasagam,Cherukula, Kondareddy,Park, In-Kyu,Kim, Yeu-Chun Elsevier 2018 Journal of controlled release Vol.280 No.-
<P><B>Abstract</B></P> <P>In this study, we developed novel hyaluronic acid cross-linked zein nanogels (HA-Zein NGs) to deliver the potential anticancer agent curcumin (CRC), a naturally occurring phytochemical drug in cancer cells. <I>In vitro</I> studies showed that they are highly compatible with the tested cell lines. They showed CD44 specific uptake in CT26 cell line more than by the CD44 receptor pre-inhibited CT26 cells. The CRC encapsulated HA-Zein NGs (HA-Zein-CRC NGs) found to exert a specific toxicity against CT26 sparing healthy normal fibroblast cells <I>in vitro</I>. The apoptotic effects were further confirmed with flow cytometry showing that the HA-Zein-CRC NGs exhibited high anticancer activity against the CT26 cells. The <I>in vivo</I> bio-distribution with a CT26 tumor model showed their high tumor accumulation thereby improved antitumor efficacy with a low dosage of CRC, compared to the previous reports. Thus, the preclinical studies clearly showed that these novel HA-Zein NGs would be highly beneficial in encapsulating hydrophobic drugs with improved pharmacokinetics thereby enhancing the therapeutic outcomes.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Yoo, Jisang,Lee, DaeYong,Gujrati, Vipul,Rejinold, N. Sanoj,Lekshmi, Kamali Manickavasagam,Uthaman, Saji,Jeong, Chanuk,Park, In-Kyu,Jon, Sangyong,Kim, Yeu-Chun Elsevier 2017 Journal of controlled release Vol.246 No.-
<P><B>Abstract</B></P> <P>Cell-penetrating peptides (CPPs) have been widely used to deliver nucleic acid molecules. Generally, CPPs consisting of short amino acid sequences have a linear structure, resulting in a weak complexation and low transfection efficacy. To overcome these drawbacks, a novel type of CPP is required to enhance the delivery efficacy while maintaining its safe use at the same time.</P> <P>Herein, we report that a bioreducible branched poly-CPP structure capable of responding to reducing conditions attained both outstanding delivery effectiveness and selective gene release in carcinoma cells. Branched structures provide unusually strong electrostatic attraction between DNA and siRNA molecules, thereby improving the transfection capability through a tightly condensed form. We designed a modified type of nona-arginine (mR9) and synthesized a branched-mR9 (B-mR9) using disulfide bonds. A novel B-mR9/pDNA polyplex exhibited redox-cleavability and high transfection efficacy compared to conventional CPPs, with higher cell viability as well. B-mR9/VEGF siRNA polyplex exhibited significant serum stability and high gene-silencing effects <I>in vitro</I>. Furthermore, the B-mR9 polyplex showed outstanding tumor accumulation and inhibition ability <I>in vivo</I>. The results suggest that the bioreducible branched poly CPP has great potential as a gene delivery platform.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Arun, Muthukrishnan,Subramanyam, Kondeti,Mariashibu, Thankaraj Salammal,Theboral, Jeevaraj,Shivanandhan, Ganeshan,Manickavasagam, Markandan,Ganapathi, Andy Humana Press 2015 Applied biochemistry and biotechnology Vol.175 No.4
<P>Soybean is a recalcitrant crop to Agrobacterium-mediated genetic transformation. Development of highly efficient, reproducible, and genotype-independent transformation protocol is highly desirable for soybean genetic improvement. Hence, an improved Agrobacterium-mediated genetic transformation protocol has been developed for cultivar PK 416 by evaluating various parameters including Agrobacterium tumefaciens strains (LBA4404, EHA101, and EHA105 harboring pCAMBIA1304 plasmid), sonication duration, vacuum infiltration pressure, and vacuum duration using cotyledonary node explants of soybean prepared from 7-day-old seedlings. The transformed plants were successfully developed through direct organogenesis system. Transgene expression was assessed by GUS histochemical and gfp visual assays, and integration was analyzed by PCR and Southern blot hybridization. Among the different combinations and durations evaluated, a maximum transformation efficiency of 18.6 % was achieved when the cotyledonary node explants of cv. PK 416 were sonicated for 20 s and vacuum infiltered for 2 min at 250 mmHg in A. tumefaciens EHA105 suspension. The amenability of the standardized protocol was tested on four more soybean cultivars JS 90-41, Hara Soy, Co 1, and Co 2 in which all the cultivars responded favorably with transformation efficiency ranging from 13.3 to 16.6 %. The transformation protocol developed in the present study would be useful to transform diverse soybean cultivars with desirable traits.</P>
Vasudevan Venkatachalam,Sathish Dorairaj,Ajithan Chandrasekaran,Sathish Selvam,Manickavasagam Markandan 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4
The production of transgenic watermelon through Agrobacterium tumefaciens-mediated transformation with in vitro regen- eration system is a time-consuming, labor-intensive and genotype-dependent process. To acquire large number of transgenic watermelons in a shorter period of time, the half-seed explants were infected with Agrobacterium strain EHA 105 harboring pCAMBIA1301 with bar and gus genes. The factors affecting in planta transformation efficiency, such as co-cultivation duration, acetosyringone concentration, sonication duration and vacuum infiltration were assessed in the present study. The half-seed explants were sonicated for 3 min and 2 min vacuum infiltrated in Agrobacterium suspension and co-cultivated for 3 days with 100 µM acetosyringone showed maximum transformation efficiency. The transformed watermelon plants were selected against BASTA® and GUS, PCR, Southern hybridization analysis confirmed the transgene integration. The amenability of this established protocol was analyzed on four genotypes, in which the response of all genotypes was posi- tive, whereas Arka manik showed the higher transformation efficiency of 17%. The transformation protocol developed in the present study is efficient, economical and expeditious without the taking part of any tissue culture phases and produce a large number of transgenic lines within a short period of 56 days.