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RISS 인기검색어
- 검색키워드
- 저자 : Markandan Manickavasagam (검색결과 3 건)
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Selvam Sathish,Venkatachalam Vasudevan,Sivabalan Karthik,Gadamchetty Pavan,Markandan Manickavasagam 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.6
Hybanthus enneaspermus is an important source for L-Dopa production. This study reports elicited L-Dopa production in cell suspension cultures of Hybanthus enneaspermus. Cell suspension culture was established using green friable calli from leaf explants cultured on MS medium containing 2.0 mg/l 2,4-D. Effects of different elicitors such as SA, YE, MeJA and AgNO3 on biomass accumulation and L-Dopa content were studied. Among the elicitor tested SA treated culture produced highest biomass and L-Dopa according to their exposure time and concentration. Maximum biomass of 15.5 ± 0.16 g FW, 4.05 ± 0.18 g DW and L-Dopa production of 8.88 mg/g DW were observed at 150 μM concentration of SA. This was 9.25-fold higher compared to that of the unelicited control culture. The results obtained in this study clearly show that the elicita-tion strategy is a promising method for biosynthesis of L-Dopa production by cell suspension cultures of H. enneaspermus.
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Arun, Muthukrishnan,Subramanyam, Kondeti,Mariashibu, Thankaraj Salammal,Theboral, Jeevaraj,Shivanandhan, Ganeshan,Manickavasagam, Markandan,Ganapathi, Andy Humana Press 2015 Applied biochemistry and biotechnology Vol.175 No.4
<P>Soybean is a recalcitrant crop to Agrobacterium-mediated genetic transformation. Development of highly efficient, reproducible, and genotype-independent transformation protocol is highly desirable for soybean genetic improvement. Hence, an improved Agrobacterium-mediated genetic transformation protocol has been developed for cultivar PK 416 by evaluating various parameters including Agrobacterium tumefaciens strains (LBA4404, EHA101, and EHA105 harboring pCAMBIA1304 plasmid), sonication duration, vacuum infiltration pressure, and vacuum duration using cotyledonary node explants of soybean prepared from 7-day-old seedlings. The transformed plants were successfully developed through direct organogenesis system. Transgene expression was assessed by GUS histochemical and gfp visual assays, and integration was analyzed by PCR and Southern blot hybridization. Among the different combinations and durations evaluated, a maximum transformation efficiency of 18.6 % was achieved when the cotyledonary node explants of cv. PK 416 were sonicated for 20 s and vacuum infiltered for 2 min at 250 mmHg in A. tumefaciens EHA105 suspension. The amenability of the standardized protocol was tested on four more soybean cultivars JS 90-41, Hara Soy, Co 1, and Co 2 in which all the cultivars responded favorably with transformation efficiency ranging from 13.3 to 16.6 %. The transformation protocol developed in the present study would be useful to transform diverse soybean cultivars with desirable traits.</P>
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Vasudevan Venkatachalam,Sathish Dorairaj,Ajithan Chandrasekaran,Sathish Selvam,Manickavasagam Markandan 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4
The production of transgenic watermelon through Agrobacterium tumefaciens-mediated transformation with in vitro regen- eration system is a time-consuming, labor-intensive and genotype-dependent process. To acquire large number of transgenic watermelons in a shorter period of time, the half-seed explants were infected with Agrobacterium strain EHA 105 harboring pCAMBIA1301 with bar and gus genes. The factors affecting in planta transformation efficiency, such as co-cultivation duration, acetosyringone concentration, sonication duration and vacuum infiltration were assessed in the present study. The half-seed explants were sonicated for 3 min and 2 min vacuum infiltrated in Agrobacterium suspension and co-cultivated for 3 days with 100 µM acetosyringone showed maximum transformation efficiency. The transformed watermelon plants were selected against BASTA® and GUS, PCR, Southern hybridization analysis confirmed the transgene integration. The amenability of this established protocol was analyzed on four genotypes, in which the response of all genotypes was posi- tive, whereas Arka manik showed the higher transformation efficiency of 17%. The transformation protocol developed in the present study is efficient, economical and expeditious without the taking part of any tissue culture phases and produce a large number of transgenic lines within a short period of 56 days.
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