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Kamachi, Hiroyuki,Noguchi, Munenori,Inoue, Hiroshi 한국식물학회 2004 Journal of Plant Biology Vol.47 No.1
Fern gametophyte is a good model system to investigate signal transduction in plant cells. In this work, we examined whether CDPKs are involved in the mechanisms of spore germination of the fern Osmunda japonica. A protein extract from the spores included four CDPK isoforms with relative molecular weights of 56,53,49, and 47 kDa, as detected by immunoblot analysis, and they showed CDPK-like activities, as detected by in-gel protein-kinase assay. It was also found that the inhibitors effective on CDPKs, such as a general protein kinase inhibitor, K252a, and a calmodulin antagonist, W-7, largely suppressed the spore germination, and that many proteins of the spores were phosphorylated in vivo in a calcium dependent manner in the period when the spores require external Ca^(2+) for the germination. Furthermore, we showed that Sr^(2+) and Mn^(2+) , which could substitute for Ca^(2+) in the spore germination, were also able to activate the Osmunda CDPKs. From these results, we concluded that CDPKs would participate in the spore germination of O. japnica.
Seo, Mi ,Sook,Kamachi, Takashi,Kouno, Tomohisa,Murata, Koji,Park, Mi ,Joo,Yoshizawa, Kazunari,Nam, Wonwoo WILEY-VCH Verlag 2007 Angewandte Chemie Vol.46 No.13
<B>Graphic Abstract</B> <P>The active oxidant that oxygenates organic substrates is an iron(IV) oxo intermediate, namely, [(tpa)Fe<SUP>IV</SUP>(O)(CH<SUB>3</SUB>CN)]<SUP>2+</SUP> (2), which is formed through O&n.bond;O bond homolysis, according to experimental and computational studies on the reactivity of nonheme iron(III) alkylperoxo complex [(tpa)Fe<SUP>III</SUP>(OOtBu)(CH<SUB>3</SUB>CN)]<SUP>2+</SUP> (1), which was shown to be a sluggish electrophilic oxidant that is incapable of oxygenating organic substrates. tpa=tris(2-pyridylmethyl)amine. <img src='wiley_img/14337851-2007-46-13-ANIE200604219-content.gif' alt='wiley_img/14337851-2007-46-13-ANIE200604219-content'> </P>
D. Ramachandran,R. P. George,Vinita Vishwakarma,U. Kamachi Mudali 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.4
The objective of this work was to get a holistic idea about the behavior of fly ash concrete exposed to sea water environments. Concrete mixes namely Normal Concrete (NC), concrete with fly ash and superplasticizer (FA) and concrete with superplasticizer alone (SP) were cast and immersed for a year to find out the strength, durability, biofilms formation and chemical composition. The pH, compressive strength, Half Cell Potential (HCP), Rapid Chloride Permeability Test (RCPT) and carbonation test showed better results in FA. Epifluorescence micrograph observed less number of fluorescing cells. Analysis of phase images of Lock- in thermography reveals the phase angle differences of concrete samples indicating the least deterioration on FA concrete. After 280 days of exposure, XRD showed higher loss of calcium elements in NC and SP concrete compared to FA. LRS also showed that Raman intensities of NC and SP were drastically reduced. However, LRS and XRD results of FA confirmed formation of more cementitious products due to secondary hydration explaining the reason for increased compressive strength.