Apoptosis-inducing effect and structural basis of Polygonatum cyrtonema Lectin and chemical modification properties on its mannose-binding sites

( Bo Liu ),( Xiao Chao Xu ),( Yan Cheng ),( Jian Huang ),( Yan Hong Liu ),( Zhen Liu ),( Ming Wei Min ),( He Jiao Bian ),( Jing Chen ),( Jin Ku Bao ) 생화학분자생물학회 2008 BMB Reports Vol.41 No.5

ZnO Nanowires/ CdS Nanorods Structure Grown on Silica Micropillars Array for Photosensitive Application

Jing Liu,Yuanze Xu,Xiaoxiao Liang,Mingming Yan,Bo Wang,Tianchong Zhang,Zhen Hong,Futing Yi 대한금속·재료학회 2021 ELECTRONIC MATERIALS LETTERS Vol.17 No.6

Utilizing silica micropillar arrays as substrate, a kind of Zinc oxide (ZnO) nanowires/Cadmium sulfi de (CdS) nanopillars heterojunctionstructure is prepared successfully by twice hydrothermal reaction method. This ZnO nanowires/CdS nanorods/silicamicropillars structure is tree-liked morphology from the SEM and TEM images, and both the ZnO nanowires and CdS nanorodsare well crystallinity on the silica surface from the X-ray diff raction curves. The CdS nanorods/silica micropillars structure has alow refl ectivity to the incoming light for wavelength from 200 to 800 nm. After the ZnO nanowires growing, the refl ectivity oftree-liked structure is much lower than that without ZnO nanowires especially for the wavelength less than 350 nm. The resistanceresponse of the CdS nanorods/silica micropillars based photoresistors with and without ZnO nanowires are tested. And theresults reveal that the ZnO nanowires covering can improve the response of the CdS nanorods/silica micropillars photoresistors. The best photosensitivity response of the ZnO nanowires/CdS nanorods/silica micropillars photoresistor for 450 nm wavelengthlight with 5 mW/cm 2 illumination can achieve to 23.3, while that for CdS nanorods/silica micropillars photoresistors is 18.8.

Analysis of Geographic and Pairwise Distances among Chinese Cashmere Goat Populations

Liu, Jian-Bin,Wang, Fan,Lang, Xia,Zha, Xi,Sun, Xiao-Ping,Yue, Yao-Jing,Feng, Rui-Lin,Yang, Bo-Hui,Guo, Jian Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3

This study investigated the geographic and pairwise distances of nine Chinese local Cashmere goat populations through the analysis of 20 microsatellite DNA markers. Fluorescence PCR was used to identify the markers, which were selected based on their significance as identified by the Food and Agriculture Organization of the United Nations (FAO) and the International Society for Animal Genetics (ISAG). In total, 206 alleles were detected; the average allele number was 10.30; the polymorphism information content of loci ranged from 0.5213 to 0.7582; the number of effective alleles ranged from 4.0484 to 4.6178; the observed heterozygosity was from 0.5023 to 0.5602 for the practical sample; the expected heterozygosity ranged from 0.5783 to 0.6464; and Allelic richness ranged from 4.7551 to 8.0693. These results indicated that Chinese Cashmere goat populations exhibited rich genetic diversity. Further, the Wright's F-statistics of subpopulation within total (FST) was 0.1184; the genetic differentiation coefficient (GST) was 0.0940; and the average gene flow (Nm) was 2.0415. All pairwise FST values among the populations were highly significant (p<0.01 or p<0.001), suggesting that the populations studied should all be considered to be separate breeds. Finally, the clustering analysis divided the Chinese Cashmere goat populations into at least four clusters, with the Hexi and Yashan goat populations alone in one cluster. These results have provided useful, practical, and important information for the future of Chinese Cashmere goat breeding.

Long noncoding RNA UCA1 inhibits ischaemia/reperfusion injury induced cardiomyocytes apoptosis via suppression of endoplasmic reticulum stress

Jing Chen,Qi Hu,Bo‑fang Zhang,Xiao‑pei Liu,Shuo Yang,Hong Jiang 한국유전학회 2019 Genes & Genomics Vol.41 No.7

Background Ischemia heart disease is one of the major causes of death worldwide which often associated with tissue infarction and limit the recovery of function. Multiple factors involved in the I/R-induced cardiomyocyte dysfunction which were consistent with a role of oxidative stress and altered endothelium-dependent responses. However, the pathogenic mechanisms in I/R injury remain unclear. Materials and methods The H9C2 cells were in the ischaemia/reperfusion (I/R) condition. After I/R, the cells were transfected with or without adenovirus-urothelial carcinoma associated 1(Ad-UCA1). Then qRT-PCR analysis was performed to quantify mRNA expression of different treatment groups. Cell apoptosis rate was assessed using flow cytometry and ER stress biomarker expression were measured by immunoblotting. Intracellular and mitochondrial ROS generation were assayed by fluorescence microscope after staining with the DCFDA or MitoSOX. Results I/R conditions trigger lncRNAs UCA1 expression, cellular and mitochondria ROS production, resulting in cell apoptosis through the induction of oxidative and ER stress. Overexpression of UCA1 protects H9C2 cells from I/R-induced ER stress and cell apoptosis. Moreover, UCA1 might be a potential regulator in the protective effect of I/R‑induced oxidative stress and mitochondria dysfunction. Subsequently, ER stress inhibitor attenuated the effect of siUCA1 induced injury in H9C2 cells. Conclusion The expression of UCA1 against I/R induced oxidative stress and mitochondria dysfunction via suppression of endoplasmic reticulum stress. UCA1 might be a biomarker to improved diagnosis of I/R injury.

Research on stochastic stability and stochastic bifurcation of suspended wheelset

Bo Zhang,Jing Zeng,Weiwei Liu 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.8

We studied the stochastic stability and bifurcation behavior for a suspended wheelset system in the presence of a Gauss white noisestochastic parametric excitation. First, the global stochastic stability was researched by judging the modality of the singular boundary. Then, the diffusion exponent, drift exponent and character value of the two boundaries were calculated. After getting the maximal Lyapunovexponent, the condition of D-bifurcation was obtained. By analyzing the shape and peaks of the stationary probability densityfunction, the condition of stochastic P-bifurcation was also obtained. Finally, the numerical verification and the comparison betweendeterministic bifurcation and P-bifurcation were performed. The results show that the random excitation shifts the critical velocity to alower value, and the stochastic system becomes more sensitive and more unstable. The stochastic parametric excitation can destroy theorigin subcritical Hopf bifurcation in the deterministic system.

Development of a Novel FES Control System Based on Treadmill Motor Current Variation for Gait Rehabilitation of Hemiplegic Patients after Stroke

Jing Ye,Yasutaka Nakashima,Takao Watanabe,Masatoshi Seki,Bo Zhang,Quanquan Liu,Yuki Yokoo,Yo Kobayashi,Qixin Cao,Masakatsu G. Fujie 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10

In consideration of a large proportion of stroke survivors with persistent deficits, current interventions have limited ability to restore normal motor function. We propose a gait training system for gait rehabilitation of hemiplegic patients with convalescent stroke. It consists of a functional electrical stimulation (FES) device which is used to influence the gait pattern instantly to improve the quality of the gait, and a treadmill with two separated belts. The stimulus triggers from the FES could be controlled automatically by the subject`s gait phase determination, including stance phase and swing phase. Gait phase could be estimated and recognized through observing current value variation of the treadmill motor during the subject’s walking on the treadmill belts. In this paper, we have preliminarily tested the feasibility of the proposed method through hemiplegic simulation experiments. Also, Tibialis Anterior (TA) and quadriceps on the hemiplegic side were successfully stimulated by the expected FES stimuli during gait.

Gene Polymorphism of XRCC1 Arg399Gln and Cervical Carcinoma Susceptibility in Asians: A Meta-analysis Based on 1,759 Cases and 2,497 Controls

Liu, Yi-Ting,Shi, Jing-Pu,Fu, Ling-Yu,Zhou, Bo,Wang, Hai-Long,Wu, Xiao-Mei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Many epidemiological studies in Asian populations have investigated associations between the Arg399Gln gene polymorphism of X-ray repair cross complementing gene 1 (XRCC1) and risk of cervical carcinoma, but no conclusions have been available because of controversial results. Therefore a meta-analysis was conducted for clarification. Relevant studies were identified by searching the Pubmed, Embase, the Web of Science, Cochrane Collaboration's database, Chinese National Knowledge Infrastructure (CNKI), Wanfang database and China Biological Medicinse (CBM) until September, 2012. A total of eight studies were included in the present meta-analysis, which described 1,759 cervical carcinoma cases and 2,497 controls. Odds ratios (ORs) and corresponding 95% confidence intervals (95%CIs) as effect size were calculated by fixed-effect or random-effect models. The overall results indicated that the XRCC1-399G/A polymorphism was marginally associated with cervical carcinoma in Asians: OR (95%CI): 1.16 (1.07, 1.26) in the G/A vs G/G inheritance model, 1.24 (0.87, 1.76)in A/A vs G/G inheritance model, 1.13 (1.01, 1.27) in the dominant inheritance model and 1.18 (0.94, 1.47) in the recessive inheritance model. Subgroup analyses on sample size showed no significant correlation in the small-sample size group but the large-sample size group was consistent with the outcomes of overall meta-analysis. In the subgroup analysis by regions, we only found significant association under the G/A vs G/G inheritance model in the Chinese population. For the non-Chinese populations, no correlation was detected in any genetic inheritance model. In the Asian populations, XRCC1-399G/A gene polymorphism was implied to be associated with cervical carcinoma.

Label-Free Fluorescent Detection of Hg2+ in Aqueous Media Based on N-Doped MoS2 Nanosheets

Jing Liu,Xiao Li,Xin Gong,Taiping Qing,Peng Zhang,Bo Feng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.5

The incorporation of dopant atoms alters the structure of MoS2, resulting in unique properties and practical applications. Herein, a facile and low-cost approach for producing N-doped molybdenum disulfide (N-MoS2) was carried out using melamine and MoS2 in a laboratory tube furnace at 400 ℃ for 2h. The N-MoS2 fluorescent probe showed sensitive response to Hg2+ in a wide concentration range of 0.4–10 μM with a low limit of 28 nM. The practical applicability of detecting Hg2+ in lake water samples may open a new way to evaluate Hg2+ in wastewater.

Multiband and Broadband Absorption Enhancement of Monolayer Graphene at Optical Frequencies from Multiple Magnetic Dipole Resonances in Metamaterials

Liu, Bo,Tang, Chaojun,Chen, Jing,Xie, Ningyan,Tang, Huang,Zhu, Xiaoqin,Park, Gun-sik Springer US 2018 NANOSCALE RESEARCH LETTERS Vol.13 No.1

<P>It is well known that a suspended monolayer graphene has a weak light absorption efficiency of about 2.3% at normal incidence, which is disadvantageous to some applications in optoelectronic devices. In this work, we will numerically study multiband and broadband absorption enhancement of monolayer graphene over the whole visible spectrum, due to multiple magnetic dipole resonances in metamaterials. The unit cell of the metamaterials is composed of a graphene monolayer sandwiched between four Ag nanodisks with different diameters and a SiO<SUB>2</SUB> spacer on an Ag substrate. The near-field plasmon hybridizations between individual Ag nanodisks and the Ag substrate form four independent magnetic dipole modes, which result into multiband absorption enhancement of monolayer graphene at optical frequencies. When the resonance wavelengths of the magnetic dipole modes are tuned to approach one another by changing the diameters of the Ag nanodisks, a broadband absorption enhancement can be achieved. The position of the absorption band in monolayer graphene can be also controlled by varying the thickness of the SiO<SUB>2</SUB> spacer or the distance between the Ag nanodisks. Our designed graphene light absorber may find some potential applications in optoelectronic devices, such as photodetectors.</P>

Elevating Limonene Production in Oleaginous Yeast Yarrowia lipolytica via Genetic Engineering of Limonene Biosynthesis Pathway and Optimization of Medium Composition

Bo-Qian Cheng,Liu-Jing Wei,Yu-Bei Lv,Jun Chen,Qiang Hua 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.3

As an alternative terpenoid producer, nonconventional oleaginous yeast Yarrowia lipolytica was chosen for limonene production. Y. lipolytica can give high biomass yield and metabolize a broad range of substrates such as glycerol, alkanes, fatty acid, fats, and oils. As previously reported, optimization of limonene synthesis pathway and mevalonate (MVA) pathway leads to the accumulation of 112-fold higher limonene as compared to an initial strain. In this study, we introduced an additional copy of limonene synthesis gene (LS), which resulted in an increase of limonene production. This engineered strain was used to carry out further optimization study. Amongst all the carbon sources tested, the highest level of limonene production was obtained from glycerol, and citrate was selected as an auxiliary carbon source. In fed-batch fermentation with an optimized medium, the engineered strain was found to produce 165.3 mg/L limonene, which corresponds to the highest yield till date for the production of limonene in Y. lipolytica.