Inhibition of lncRNA KCNQ1OT1 Improves Apoptosis and Chemotherapy Drug Response in Small Cell Lung Cancer by TGF-β1 Mediated Epithelial-to-Mesenchymal Transition

Deyu Li,Qin Tong,Yuane Lian,Zhizhong Chen,Yaru Zhu,Weimei Huang,Yang Wen,Qiongyao Wang,Shumei Liang,Man Li,Jianjing Zheng,Zhenhua Liu,Huanxin Liu,Linlang Guo 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Drug resistance is one of the main causes of chemotherapy failure in patients with small cell lung cancer (SCLC), and extensive biological studies into chemotherapy drug resistance are required. Materials and Methods In this study, we performed lncRNA microarray, in vitro functional assays, in vivo models and cDNA microarray to evaluate the impact of lncRNA in SCLC chemoresistance. Results The results showed that KCNQ1OT1 expression was upregulated in SCLC tissues and was a poor prognostic factor for patients with SCLC. Knockdown of KCNQ1OT1 inhibited cell proliferation, migration, chemoresistance and promoted apoptosis of SCLC cells. Mechanistic investigation showed that KCNQ1OT1 can activate transforming growth factor-β1 mediated epithelial-to-mesenchymal transition in SCLC cells. Conclusion Taken together, our study revealed the role of KCNQ1OT1 in the progression and chemoresistance of SCLC, and suggested KCNQ1OT1 as a potential diagnostic and prognostic biomarker in SCLC clinical management.

Speed and flux observer based on adaptive linear neuron for induction motor

Liangbin Liu,Deyu Liu 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.9

The robustness and economy of the induction motor drive system can be improved by a high-precision observation algorithm instead of an encoder. Such an algorithm is adopted to obtain the speed and flux linkage of the induction motor. In this paper, an adaptive linear neuron speed digital observer for the induction motor is proposed. First, discrete voltage and current models are established. Then, a novel speed and rotor flux digital observer based on the adaptive linear neuron with less calculation complexity is presented. This observer contains a hidden layer and two input signals that can work with the rotor flux error and the deviation of the error. The adaptive learning method of the weight coefficients described by the difference equation is presented. Finally, a simulation and an experiment are conducted to certify the correctness and effectiveness of the proposed adaptive linear neuron speed digital observer.

An Energy Efficient Sink Deployment Scheme aiming at extending the Lifespan for Wireless Sensor Networks

Deyu Lin,Quan Wang,Jizhao Liu 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.8

Effects of exploration and molecular mechanism of CsV on eNOS and vascular endothelial functions

Zuo, Deyu,Jiang, Heng,Yi, Shixiong,Fu, Yang,Xie, Lei,Peng, Qifeng,Liu, Pei,Zhou, Jie,Li, Xunjia Techno-Press 2022 Advances in nano research Vol.12 No.5

This study aimed to investigate the effects and potential mechanisms of Chikusetsusaponin V (CsV) on endothelial nitric oxide synthase (eNOS) and vascular endothelial cell functions. Different concentrations of CsV were added to animal models, bovine aorta endothelial cells (BAECs) and human umbilical vein endothelial cells (HUVECs) cultured in vitro. qPCR, Western blotting (WB), and B ultrasound were performed to explore the effects of CsV on mouse endothelial cell functions, vascular stiffness and cellular eNOS mRNA, protein expression and NO release. Bioinformatics analysis, network pharmacology, molecular docking and protein mass spectrometry analysis were conducted to jointly predict the upstream transcription factors of eNOS. Furthermore, pulldown and ChIP and dual luciferase assays were employed for subsequent verification. At the presence or absence of CsV stimulation, either overexpression or knockdown of purine rich element binding protein A (PURA) was conducted, and PCR assay was employed to detect PURA and eNOS mRNA expressions, Western blot was used to detect PURA and eNOS protein expressions, cell NO release and serum NO levels. Tube formation experiment was conducted to detect the tube forming capability of HUVECs cells. The animal vasodilation function test detected the vasodilation functions. Ultrasonic detection was performed to determine the mouse aortic arch pulse wave velocity to identify aortic stiffness. CsV stimulus on bovine aortic cells revealed that CsV could upregulate eNOS protein levels in vascular endothelial cells in a concentration and time dependent manner. The expression levels of eNOS mRNA and phosphorylation sites Ser1177, Ser633 and Thr495 increased significantly after CsV stimulation. Meanwhile, CsV could also enhance the tube forming capability of HUVECs cells. Following the mice were gavaged using CsV, the eNOS protein level of mouse aortic endothelial cells was upregulated in a concentration- and time-dependent manner, and serum NO release and vasodilation ability were simultaneously elevated whereas arterial stiffness was alleviated. The pulldown, ChIP and dual luciferase assays demonstrated that PURA could bind to the eNOS promoter and facilitate the transcription of eNOS. Under the conditions of presence or absence of CsV stimulation, overexpression or knockdown of PURA indicated that the effect of CsV on vascular endothelial function and eNOS was weakened following PURA gene silence, whereas overexpression of PURA gene could enhance the effect of CsV upregulating eNOS expression. CsV could promote NO release from endothelial cells by upregulating the expression of PURA/eNOS pathway, improve endothelial cell functions, enhance vasodilation capability, and alleviate vessel stiffness. The present study plays a role in offering a theoretical basis for the development and application of CsV in vascular function improvement, and it also provides a more comprehensive understanding of the pharmacodynamics of CsV.

Identification and differential expression of two isogenes encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Glycine max

Man Zhang,Deyue Yu,Kai Li,Jianyu Liu 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4

Plant 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) were considered to be encoded by single copy. In this study, we successfully isolated two DXR isogenes, designated GmDXR1 and GmDXR2, from soybean. The multicopy nature of DXRs in soybean was supported by Southern blot. Alignment of the two GmDXRs showed the presence of the N-terminal transit peptide for plastids, the conserved cleavage site, prolinerich region, and the NADPH-binding motif. Phylogenetic analysis revealed GmDXR1 and GmDXR2 belonged to different branches of angiosperm DXRs clusters. Expression pattern analysis indicated that GmDXR1 was expressed in all analyzed organs except the pod walls, with the highest level in seeds, whereas no message was detected for GmDXR2. In response to heat stress, GmDXR1 showed declined transcript levels in the experiments; in contrast,GmDXR2 was highly responsive, with mRNA accumulation peaking at 6 h after treatment. We also demonstrated that both GmDXRs were localized in chloroplasts. Overexpression of GmDXRs induced increased contents of various isoprenoids (chlorophyll, carotenoids, and gibberellins),but with reduced level of ABA, indicating that GmDXRs participate in the control of differential isoprenoid biosynthesis of the MEP pathway. This work provides a new insight into the wide presence of multicopies of DXR enzyme in plants.

Parameter Calibration of a Discrete Element Model for Three-Compartment Separation and Fragmentation of Castor Capsules During Harvesting

Hou Junming,Ren Zhaotan,Liu Deyu,Ma Zhi,Liu Xu,Wang Wei 한국농업기계학회 2023 바이오시스템공학 Vol.48 No.2

Purpose In order to analyze the damage of castor capsule during mechanical harvesting, it is necessary to establish a suitable model and obtain the parameters of discrete element model. Therefore, the study establishes a three-compartment separation and crushing discrete element model of castor; the contact parameters and bonding parameters of the model are calibrated. The TongBi No. 16 castor variety in harvest period was taken as the research object. The sizes of whole castor capsule and single part were measured; then, the moisture content of castor in harvest period was measured. Methods In order to obtain the inherent parameters of the capsule, the elastic modulus was measured by TMS pro professional food physical property analyzer. In order to realize the three-compartment separation and fragmentation of castor, a discrete element model combining the aggregation and bonding model was established. The contact parameters are calibrated by stacking angle and formed by lifting method. The contour curve of accumulation angle is obtained with MATLAB software. The results show that the stacking angle of physical test is 26.216°. Through Plackett–Burman test, the contact parameters that have a greater impact on the stacking angle are the static friction coefficient between castors and the dynamic friction coefficient between castors. After the steepest climbing test, the value range is selected. Finally, through response surface optimization, the optimal static and dynamic friction coefficients between castors are determined to be 0.41 and 0.05, respectively. Results In order to obtain accurate bonding parameters, it is carried out through compression test. In order to reduce the damage to single compartment in the process of compression, spherical compression head is used. The results show that the maximum destructive force is 52.65 N. In conducting the simulation tests, the Box-Behnken response surface design is applied to derive the optimal bonding parameters, the normal stiffness per unit area is 9.47× 107 N/m3, shear stiffness per unit area is 4.59× 107 N/m3, normal strength is 7.92× 103 Pa, and shear strength is 1.13× 104 Pa. Finally, the stacking angle test and cone compression test are carried out by the final calibrated parameters. The relative errors between the simulation and the actual test are 1.82% and 4.10%, respectively, which indicate that the calibration results are reliable. Conclusion This study can provide the support of discrete element simulation parameters for analyzing the mechanism of castor capsule damage during mechanized harvest.

Genomic, evolutionary and expression profile analysis of Hsp70 superfamily in A and D genome of cotton (Gossypium spp.) under the challenge of Verticillium dahliae

Songhua Xiao,Jianwen Xu;Jun Zhao;Jianguang Liu,Jianwen Xu;Jun Zhao;Jianguang Liu,Qiaojuan Wu,Jingzhong Yu,Deyue Yu 한국식물학회 2017 Journal of Plant Biology Vol.60 No.1

In this study, we comparatively analyzed the 115 Hsp70 genes identified in Gossypium raimondii, Gossypium hirsutum and Gossypium arboreum genomes. Those Hsp70 genes unequally distributed among chromosomes in A and D genome of cotton (Gossypium spp.), and were classified into 29 groups according to the homology of them. Based on the localization information of the orthologs in Arabidopsis, the Hsp70 proteins were predicted to locate in cytosol, endoplasmic reticulum, mitochondrion or chloroplast. Homologous analysis indicated the evolutionary conservation of Hsp70 in cotton. In addition, those Hsp70 genes were differently expressed in Suyuan-045, Hai-7124 and TM-1, which were highly resistant, resistant, and sensitive to Verticillium dahliae respectively. The expressions of 26 Hsp70 genes were induced by Verticillium dahliae except for Hsp70-07/16/25/26, and the result suggested the potential involvement of them in responding to Verticillium wilt. Hsp70-08/30/31 was highly expressed in both Suyuan-045 and Hai-7124, and it was hypothesized that they might be involved in the resistance to the invasion of Verticillium dahliae. 144h after inoculation with Verticillium dahliae, the expression of Hsp70-13/14/15 was only up-regulated in Suyuan-045, and it was assumed that they might be involved in resistance to the extension of Verticillium dahliae. Further study on those Hsp70 genes would be valuable to reveal the role of them in Verticillium wilt resistance.

A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus

Yanqiu Wei,Baozhi Yang,Yunlong Li,Yongcheng Duan,Deyu Tian,Baoxiang He,Chuangfu Chen,Wenjun Liu,Limin Yang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.4

A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.

[가솔린엔진] 밸브개폐시기가변에 따른 엔진 특성의 예측에 관한 연구

황재원(J.W. Hwang),김만호(M.H. Kim),박재근(J.K. Park),Wu deyu,Liu Shenghua,채재우(J.O. Chae) 한국자동차공학회 1999 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

In this paper, a zero-dimensional two zone model is developed to investigate the effects of variable valve timing on combustion process in SI engine. The simulation results show that the predicted data has good agreement with experimental ones. The useful information of combustion process such like residual gas fraction, cylinder pressure, cylinder temperature and NO concentration can be obtained and the effects of engine variables on combustion processes and performances can be evaluated

밸브개폐시기가변에 따른 엔진 특성의 예측에 관한 연구

황재원(J.W.Hwang),김민호(M.H.Kirn),박재근(J.K.Park),Wu deyu,Liu Shenghua,채재우(J.O.Chae) 한국자동차공학회 1999 한국 자동차공학회논문집 Vol.7 No.9