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Ling‑Hong Miao,Wen‑Jing Pan,Yan Lin,Bo Liu,Ming‑Chun Ren,Qun‑Lan Zhou,Xian‑Ping Ge 한국유전학회 2017 Genes & Genomics Vol.39 No.12
The objective of this study was to analyze the target genes and regulatory function of miR-34a in Megalobrama amblycephala using second-generation highthroughput sequencing and bioinformatic tools. Functional enrichment analysis was performed by gene ontology. MiR- 34a and target gene expression levels were measured in M. amblycephala fed normal and high-carbohydrate diets. The results revealed that miR-34a was highly conserved in several species, and miR-34a of M. amblycephala has a close evolutionary relationship to that of zebrafish and common carp. miRanda, TargetScan, RNAhybrid predicted 5,185, 6,282 and 2,168 target genes, respectively, and 645 target genes were in common. According to annotation information, the target genes were enriched in phosphate metabolism, glycerophospholipid metabolism, Golgi vesicle transport, cell division, and other biological processes (P < 0.05). Pathway enrichment analysis revealed that these target genes were mainly enriched in alpha-linolenic acid and linoleic acid metabolism, ether lipid metabolism, VEGF signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, and MAPK signaling pathway (P < 0.05). The regulatory role of miR-34a was more significant in the liver than in the brain of M. amblycephala. MiR-34a regulates glucose lipid homeostasis induced by high glucose diets by upregulating hepatic PI3K/Akt, FOXO, and TOR signaling pathways.
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Development and Application of Tea Saponins: A Review
Ling-Yan Jia,You-Ying Tu 한국차학회 2015 한국차학회지 Vol.- No.S
Tea saponins are triterpenoid saponins from Camellia plants, and consist of a sapogenin, glycosides and organic acids. Up to 2013, at least 93 tea saponin monomers from the seeds, roots and flowers of tea plants have been isolated and identifined. Tea saponins are excellent natural biosurfactant, and possess a variety of pharmacological activities such as anti-inflammatory, anti-fungal, anti-bacterial, anti-cancer, hypolipidemic and gastric mucosal protection. In addition, tea saponins can be used for the recovery of heavy metals from wastewater and contaminated soils due to their high binding capacity with metal ions. These properties make tea saponins can be applied in pesticides, feed, aquaculture, textile, pharmaceuticals, cosmetics, building materials and environmental modification.Therefore, this article reviews the latest studies on tea saponins, and discuss the comprehensive utilization of tea saponins in the future
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Yan-Ling Luo,Yan Miao,Feng Xu 한국고분자학회 2011 Macromolecular Research Vol.19 No.12
Two types of polyurethanes with alternating and random block architectures, hydroxyl-terminated liquid polybutadiene and poly(ethylene glycol) block copolymers (HTPB-alt-PEG and HTPB-co-PEG), were synthesized using a coupling reaction route between the hydroxyl groups and the isocyanate groups. The chemical and crystal structures were characterized using Fourier transform-infrared spectroscopy (FTIR) and X-ray diffraction, while phase behavior was examined using scanning electron microscopy (SEM) and differential scanning calorimetry. The biodegradation in a simulated human body fluid was investigated through mass loss, SEM, and FTIR. The experimental results indicated that all of the polyurethane samples bore the microphase separation structure, and the separation degree depended on the sequence structure and molecular weight (MW) of PEG and further affected their in vitro degradation. The driving force was related to the restricted movement of the molecular segments, the crystallization of the soft/hard phases, and/or the hydrogen bonding interactions between the hard segments. The surface morphological change of the degraded samples further demonstrated that the degradation became serious as the PEG MW increased and that the random block copolymers decomposed more easily than the alternating copolymers. The block polymer materials are expected to be incorporated into specific applications in related biomedical fields.
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Yan-Ling Luo,Li-Li Chen,Feng Xu,Qiang-Suo Feng 대한금속·재료학회 2012 METALS AND MATERIALS International Vol.18 No.5
Well-dispersed copper nanoparticles were fabricated using poly(vinyl alcohol)/polyacrylamide interpenetrating polymer networks (PVA/PAAm IPNs) as a nanoreactor template. The synthesis of the IPNs hydrogels was achieved in the presence of glutaraldehyde and N,N'-methylene-bis-acrylamide. The resulting PVA/PAAm/Cu nanocomposite hydrogels were characterized, and the swelling and mechanical properties were investigated. The results indicated that the copper nanoparticles had a spherical shape with a size range from 10 to 20 nm. The complexation of PVA in PVA/PAAm IPNs with Cu 2+played an important role in avoiding the aggregation of copper nanoparticles and providing particle size and size distribution controllability and stability. Although the swelling capacity of the nanocomposite hydrogels was slightly lower than that of the control, they had better compression mechanical properties. The water uptake and mechanical properties can be easily tuned by changing the component ratios to meet the requirements of specific applications, such as drug controlled release or tissue engineering.
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Synthesis of Composite Particles with Fe3O4 core and Ag Shell for the Development of Fingerprints
Ling-Yan Zhang,Ting Chu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.5
The Fe3O4 -core and Ag- shell (Fe3O4@Ag nanoeggs) were prepared through the encapsulation of 3- aminopropyltriethoxysilane-coated magnetite nanoparticle in nano-Ag shell by a simple chemically controlled procedure. The Fe3O4@Ag nanoeggs were characterized by scanning electron microscopy, transmission electron microscopy, UV-vis spectrum and superconducting quantum interference device magnetometer, respectively. A detailed analysis is provided of how the hydrolysis and condensation of 3-aminopropyltriethoxysilane and the pH value are vital in fabricating the Fe3O4@Ag nanoeggs. The prepared Fe3O4@Ag nanoeggs possessed uniform size, improved monodispersity, stability against aggregation and high magnetization, which were utilized for the detection of latent fingerprints deposited onto different surfaces. The experimental results showed that the latent fingerprints developed with the Fe3O4@Ag nanoeggs powders exhibited excellent ridge details with minimal background staining.
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New Iterative Process for The Equation Involving Strongly Accretive Operators in Banach Spaces
Ling-yan Zeng,Jun Li,김종규 대한수학회 2007 대한수학회보 Vol.44 No.4
In this paper, under suitable conditions, we show that thenew class of iterative process with errors introduced by Li et al convergesstrongly to the unique solution of the equation involving strongly accre-tive operators in real Banach spaces. Furthermore, we prove that it isequivalent to the classical Ishikawa iterative sequence with errors.
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Node-Level Trust Evaluation Model Based on Blockchain in Ad Hoc Network
Yan, Shuai-ling,Chung, Yeongjee The Institute of Internet 2019 International journal of advanced smart convergenc Vol.8 No.4
Due to the characteristics of an ad hoc network without a control center, self-organization, and flexible topology, the trust evaluation of the nodes in the network is extremely difficult. Based on the analysis of ad hoc networks and the blockchain technology, a blockchain-based node-level trust evaluation model is proposed. The concepts of the node trust degree of the HASH list on the blockchain and the perfect reward and punishment mechanism are adopted to construct the node trust evaluation model of the ad hoc network. According to the needs of different applications the network security level can be dynamically adjusted through changes in the trust threshold. The simulation experiments demonstrate that ad-hoc on-demand distance vector(AODV) Routing protocol based on this model of multicast-AODV(MAODV) routing protocol shows a significant improvement in security compared with the traditional AODV and on-demand multipath distance vector(AOMDV) routing protocols.
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Ling Chen,Wen-jun Xiao,Qiong-xian Yan,Zhi-hua Gong,Sheng Zhang,Li Zeng,Ming Yang,Yan-he Zhou 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.8
The aim of this study is to evaluate the antiinflammatory and protective eff ects of L -theanine in infl ammatorybowel disease (IBD) and to identify the underlyingmolecular mechanisms. Rats were pre-treated with L -theanineat 0, 50, 200, or 800 mg/kg/day. IBD was induced inrats using dextran sulfate sodium (DSS). Histopathologicalanalysis suggests that L -theanine can suppress DSS-inducedIBD with signifi cant inhibition of infl ammation in large andsmall intestinal tissues. Moreover, the 200 mg/kg/day L -theanine-treated DSS group had higher body and small intestineweights, a lower disease activity index and expression ofinfl ammatory factors than the DSS group without pre-treatment. In RNA sequencing and tandem mass tag labelinganalyses, large number of mRNAs and proteins expressionlevel diff ered when compared with the DSS-induced ratswith and without 200 mg/kg/day L -theanine pre-treatment. Moreover, Kyoto Encyclopedia of Genes and Genomes pathwayanalysis indicates the anti-infl ammatory activities ofL -theanine in DSS-induced IBD, with a high representationof genes in “Cholesterol metabolism” and “Retinol metabolism”pathways. Analysis of protein–protein interaction networksfurther indicates the involvement of these two pathways. These studies suggest that medium-dose L -theaninepre-treatment could ameliorate DSS-induced IBD throughmolecular mechanisms involving cholesterol and retinolmetabolism.
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