Expression and Detection of Retinol-Binding Protein-4 Gene of Pig in E. coli

LiNa Sun,WanHong Li,ShuXiong Chen,Chao Chen,XiaoFeng Hou,Yun Zhao,Lu Chen,ChunJin Li,Xu Zhou 한국동물생명공학회(구 한국동물번식학회) 2014 한국동물번식학회 한중일 심포지엄 Vol.2014 No.1

Retinol-Binding Protein-4 (RBP-4) is a low molecular weight lipocalin, which mainly functions as a carrier for vitamin A. Though liver is the main machinery for synthesis of this protein, it is also detectable in other extrahepatic tissues, for example, ovary, uterus, and placenta. Recent evidences have shown that RBP-4 plays important roles in animal reproduction, for example, promoting the development of uterus and embryo. To the best of our knowledge, our laboratory firstly reported that high level of RBP-4 existed in follicular fluid from follicular cysts in sows. Moreover, we have also found that RBP-4 could be secreted by granulosa cells, and RBP-4 receptor was detected in granulosa cells. However, there is no any evidence on the role of RBP-4 in regulating the follicular development. Therefore, cloning and expression of RBP-4 and preparation of polyclonal antibody could help us to explore the role of RBP-4 in follicular development. The aim of this work was to construct prokaryotic expression system of swine RBP-4 gene. The total RNA was extracted from swine’s normal ovarian tissue. The sequence including the whole length of RBP-4 was amplified by RT-PCR and inserted into pEASY-E1.Then transformed into E. coli BL21(DE3)pLysS after gene sequencing. Three hours later, adding IPTG with the final concentration of 1mmol/L and inducing five hours. After centrifugation, the supernatant was discarded. By adding Glucose to Luria-Bertani broth, the expressions of protein were increased. SDS-PAGE showed that the RBP-4 gene expressed in the form of inclusion body with a molecular weight of 21KD. Western-Blot results showed that the target protein could be specifically recognized by mouse anti-human monoclonal antibody. Prokaryotic expression vector of RBP-4 gene was successfully established, and the gene was successfully expressed n E. coli, which is ready for purification and RBP-4 polyclone antibody. Meanwhile, these results were beneficial to investigate the function of RBP-4 in follicular development.

Effects of maternal dietary energy restriction on laying performance, embryonic development, and lipid metabolism in broilers

Sun, Hao,Chen, Zhihui,Ma, Chengzhan,Lian, Lina,Zhao, Zeyu,Niu, Shupeng,Xu, Liangmei,Sun, Jinhua Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.5

Objective: The objective of this study was to investigate the effects of different degrees of maternal dietary energy restriction on lipid deposition in embryonic tissues during the medium laying period (37 to 39 weeks) in Arbor Acres (AA) broiler breeders. Methods: A single factor design was adopted, and 400 AA broiler breeders (20 weeks of age) with a similar weight were randomly allocated into four groups. The birds in the control group were fed a corn-soybean meal based diet, and those in trial groups were fed diets with 80%, 70%, and 50% energy levels of the basal diet. Incubated eggs from the medium laying period were collected. Samples of developing embryos at various stages were prepared for composition analysis. Results: The embryo weight in the 80% energy group was higher than those of the other groups on embryonic day (E) 13, but at 21 E, they were significantly decreased with decreasing energy intake of the broiler breeders (p<0.05). Additionally, the levels of crude fat in tissues in the restriction groups were significantly decreased (p<0.05). The long axis and area of adipocytes in breast muscle, thigh muscle and the liver were significantly decreased (p<0.05) at 21 E in the 80%, 70%, and 50% energy groups. Conclusion: The effects of the 80% maternal dietary energy restriction energy affects egg production performance, egg quality, and nutrient deposition in egg weights, which then directly impacts on the developmental process of embryos, especially on fat utilization and deposition.

Complete genome sequence of the Pogostemon cablin bacterial wilt pathogen Ralstonia solanacearum strain SY1

Sun Yunhao,Su Yutong,Hussain Ansar,Xiong Lina,Li Chunji,Zhang Jie,Meng Zhen,Dong Zhangyong,Yu Guohui 한국유전학회 2023 Genes & Genomics Vol.45 No.1

Background Ralstonia solanacearum causes bacterial wilt of Pogostemon cablin which is an important aromatic herb and also the main materials of COVID-19 therapeutic traditional drugs. However, we are lacking the information on the genomic sequences of R. solanacearum isolated from P. cablin. Objective The acquisition and analysis of this whole-genome sequence of the P. cablin bacterial wilt pathogen. Methods An R. solanacearum strain, named SY1, was isolated from infected P. cablin plants, and the complete genome sequence was sequenced and analyzed. Results The SY1 strain contains a 3.70-Mb chromosome and a 2.18-Mb megaplasmid, with GC contents of 67.57% and 67.41%, respectively. A total of 3308 predicted genes were located on the chromosome and 1657 genes were located in the megaplasmid. SY1 strain has 273 unique genes compared with five representative R. solanacearum strains, and these genes were enriched in the plant–pathogen interaction pathway. SY1 possessed a higher syntenic relationship with phylotype I strains, and the arsenal of type III effectors predicted in SY1 were also more closely related to those of phylotype I strains. SY1 contained 14 and 5 genomic islands in its chromosome and megaplasmid, respectively, and two prophage sequences in its chromosome. In addition, 215 and 130 genes were annotated as carbohydrate-active enzymes and antibiotic resistance genes, respectively. Conclusion This is the first genome-scale assembly and annotation for R. solanacearum which isolated from infected P. cablin plants. The arsenal of virulence and antibiotic resistance may as the determinants in SY1 for infection of P. cablin plants.

Research Progress and Development of Technology in Tourism Research: A Bibliometric Analysis

Zhong, Lina,Zhu, Mengyao,Sun, Sunny,Law, Rob Smart Tourism Research Center 2021 Journal of smart tourism Vol.1 No.2

The interaction between technology and tourism has been a dynamic research area recently. This study aims to review the progress and development of technology in tourism research via a bibliometric analysis. We derive the source data from the Web of Science (WoS) core collection and use CiteSpace for bibliometric analysis, including countries, institutions, authors, categories, references, and keywords. The analysis results are as follows: i) The number of published articles on the role of technology in tourism has increased in recent years. ii) Technology-related articles in tourism are abundant in Tourism Management, Journal of Travel Research, and Annals of Tourism Research. iii) The countries with the most contributions are China, the US, and the UK. The most active institutions are the Hong Kong Polytechnic University, University of Central Florida, Bournemouth University, University of Queensland, and Kyung Hee University. iv) The reference analysis results identify eight extensively researched topics from the most cited papers, and the keyword burst analysis results present an emerging trend. This study identifies the effect and development of technology in tourism research. Our findings provide implications for researchers about the current research focus of technology and the future research trend of technology in the tourism field.

Effect of Aspirin on the Expression of Hepatocyte NF-κB and Serum TNF-α in Streptozotocin-Induced Type 2 Diabetic Rats

Xiaodong Sun,Fang Han,Junling Yi,Lina Han,Ben Wang 대한의학회 2011 Journal of Korean medical science Vol.26 No.6

Aspirin is a kind of anti-inflammatory drug and may be used to reverse hyperglycemia,hyperinsulinemia, and dyslipidemia by improving insulin resistance. We hypothesized that aspirin improves insulin resistance in type 2 diabetes by inhibiting hepatic nuclear factor kappa-β (NF-κB) activation and serum tumor necrosis factor-α (TNF-α). Adult male Wistar rats were randomly divided into four groups: control, untreated diabetic, diabetic treated with metformin (100 mg /kg/day), and diabetic treated with aspirin (120 mg/kg/day). Diabetes was induced by high-fat feeding and a low dose of streptozotocin (30 mg/kg). After treatment, plasma glucose, insulin, lipids, free fatty acids (FFAs) concentrations and serum TNF-α were determined. The expression of NF-κB in hepatocytes was analyzed by immunohistochemistry and western blot. The results showed administration of aspirin caused no significant lowering in fasting glucose level but significant reduction of hepatic NF-κB expression and serum TNF-α level with improved insulin resistance compared to the diabetic group. The relevant analysis showed positive correlation between the expression of homeostasis model assessment-insulin resistance (HOMA-IR) and NF-κB (r = 0.799, P <0.01); HOMA-IR and serum TNF-α (r = 0.790, P < 0.01). It is concluded that aspirin improves insulin resistance by inhibiting hepatic NF-κB activation and TNF-α level in streptozotocin-induced type 2 diabetic rats.

Trends and all-cause mortality associated with multimorbidity of non-communicable diseases among adults in the United States, 1999-2018: a retrospective cohort study

Mengzi Sun,Ling Wang,Xuhan Wang,Li Tong,Lina Jin,Bo Li 한국역학회 2022 Epidemiology and Health Vol.45 No.-

OBJECTIVES Multimorbidity of non-communicable diseases (NCDs) has brought enormous challenges to public health, becoming a major medical burden. However, the patterns, temporal trends, and all-cause mortality associated with NCD multimorbidity over time have not been well described in the United States. METHODS All adult participants were sourced from nationally representative data from the National Health and Nutrition Examination Survey. In total, 55,081 participants were included in trend analysis, and 38,977 participants were included in Cox regression. RESULTS The 5 NCDs with the largest increases over the study period were diabetes, osteoporosis, obesity, liver conditions, and cancer. The estimated prevalence of multimorbidity increased with age, especially for middle-aged participants with 5 or more NCDs; in general, the prevalence of NCD multimorbidity was higher among females than males. Participants with 5 or more NCDs were at 4.49 times the risk of all-cause mortality of participants without any diseases. Significant interactions were found between multimorbidity and age group (p for interaction <0.001), race/ethnicity (p for interaction<0.001), and educational attainment (p for interaction=0.010). CONCLUSIONS The prevalence of multiple NCDs significantly increased from 1999 to 2018. Those with 5 or more NCDs had the highest risk of all-cause mortality, especially among the young population. The data reported by this study could serve as a reference for additional NCD research.

Cuprous Oxide Films with Hollow Cubic Cage Structure for Nonenzymatic Glucose Detection

Fang Sun,Lehong Xing,Xihui Yang,Hailiang Huang,Lina Ning 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.4

In this study, CuO films with hollow cubic cages were prepared by a facile two-step procedure consisting of electrodeposition synthesis and subsequent direct calcination. First, Cu2O nanocubes were fabricated on ITO substrate through a simple electrodeposition procedure. Then, Cu2O nanocubes were converted to CuO hollow cubic cages without obvious morphological change through direct calcination. The obtained CuO cubic cages serving as active materials illustrated a favorable performance for nonenzymatic glucose sensing with high sensitivity of 2117.44 μA mM -1 cm -2 at a low applied potential of 0.50 V, fast-response time (less than 3 s), low detection limit of 1.0 μM and wide linear range up from 2.0 μM to 1.0 mM (R2 = 0.9983). Moreover, the good selectivity of the CuO cubic cages-based nonenzymatic glucose sensor against electroactive compounds such as ascorbic acid, uric acid and dopamine were also demonstrated. These good features indicate that the as-prepared CuO cubic cages can be used as promising electrode materials, which have a great potential in the development of sensitive and selective nonenzymatic glucose sensors.

지식재산권 보호가 수입에 미치는 영향 : OECD 회원국과 비OECD 회원국간 비교

후립나(Lina Hou),장선미(Sun-Mi Chang) 한국통상정보학회 2021 통상정보연구 Vol.23 No.4

지식재산권보호가 무역에 미치는 영향은 시장확대효과(market expansion effect)와 시장지배력효과(market power effect)의 두 가지로 나눌 수 있다. 본 연구에서는 전세계 122개국가를 대상으로 지식재산권보호가 수입에 미치는 영향을 실증분석하였다. 분석연도는 2000년, 2005년, 2010년, 2015년이며, 전체국가를 OECD 회원국과 비OECD 회원국으로 구분하여 지식재산권의 보호효과에 차이가 있는지도 살펴보았다. 분석결과 전체국가를 대상으로 하였을 때는 국가의 지식재산권 보호의 수준이 높을수록 수입에 긍정적 영향을 미치는 것으로 나타나 지식재산권의 보호가 무역에 미치는 시장확대효과를 지지하는 것으로 나타났다. 국가의 규모(GDP), 개방도, R&D집중도를 모형에 추가하여 분석한 결과 국가의 규모와 개방도는 무역에 유의한 양의 영향을 미치는 것으로 나타났으나 R&D집중도의 영향은 유의하지 않은 것으로 나타났다. 이러한 효과는 OECD 회원국을 대상으로 했을 때 더욱 분명하게 나타나며, 비 OECD 회원국의 경우 지식재산권이 무역에 미치는 영향은 확실하지 않다. 또한 OECD회원국을 대상으로 규모(GDP)와 개방도와 R&D집중도 변수를 추가하여 분석한 결과 이들 변수는 모두 수입에 유의한 양의 영향을 미치는 것으로 나타났다. The impact of intellectual property right(IPR) protection on trade can be divided into two categories: the market expansion effect and the market power effect. This study empirically analyzed the impact of IPR protection on imports in 122 countries. The years of analysis are 2000, 2005, 2010, and 2015. We also examined whether there are differences in the protection effect of IPRs between OECD and non-OECD member countries. As a result of the analysis for all countries, the higher the level of national IPR protection, the more positive the impact on imports, thus supporting the market expansion effect of IPR protection on trade. As a result of analyzing the country s size(GDP), openness, and R&D intensity by adding it to the model, it was found that the country s size and openness had a significant effect on trade, but the effect of R&D intensity was not significant. This effect is more evident in OECD member countries, and the impact of intellectual property rights on trade in non-OECD member countries is unclear. In addition, as a result of analyzing the scale(GDP), degree of openness, and R&D intensity in OECD member countries, it was found that all of these variables had a significant positive effect on imports.

SMAD4 Suppresses AURKA-Induced Metastatic Phenotypes via Degradation of AURKA in a TGFβ-Independent Manner

Jia, Lina,Lee, Hun Seok,Wu, Chun Fu,Kundu, Juthika,Park, Sang Gyu,Kim, Ryong Nam,Wang, Li-Hui,Erkin, Ö,zgü,r Cem,Choi, Jong-Sun,Chae, Seoung Wan,Yang, Ho Bin,Choi, Yoon-La,Shin, Young Kee American Association for Cancer Research 2014 Molecular Cancer Research Vol.12 No.12

<P>SMAD4 has been suggested to inhibit the activity of the WNT/β-catenin signaling pathway in cancer. However, the mechanism by which SMAD4 antagonizes WNT/β-catenin signaling in cancer remains largely unknown. Aurora A kinase (AURKA), which is frequently overexpressed in cancer, increases the transcriptional activity of β-catenin/T-cell factor (TCF) complex by stabilizing β-catenin through the inhibition of GSK-3β. Here, SMAD4 modulated AURKA in a TGFβ-independent manner. Overexpression of SMAD4 significantly suppressed AURKA function, including colony formation, migration, and invasion of cell lines. In addition, SMAD4 bound to AURKA induced degradation of AURKA by the proteasome. A luciferase activity assay revealed that the transcriptional activity of the β-catenin/TCF complex was elevated by AURKA, but decreased by SMAD4 overexpression. Moreover, target gene analysis showed that SMAD4 abrogated the AURKA-mediated increase of β-catenin target genes. However, this inhibitory effect of SMAD4 was abolished by overexpression of AURKA or silencing of AURKA in SMAD4-overexpressed cells. Meanwhile, the SMAD4-mediated repression of AURKA and β-catenin was independent of TGFβ signaling because blockage of TGFβR1 or restoration of TGFβ signaling did not prevent suppression of AURKA and β-catenin signaling by SMAD4. These results indicate that the tumor-suppressive function of SMAD4 is mediated by downregulation of β-catenin transcriptional activity via AURKA degradation in a TGFβ-independent manner.</P><P><B>Implications:</B> SMAD4 interacts with AURKA and antagonizes its tumor-promoting potential, thus demonstrating a novel mechanism of tumor suppression. <I>Mol Cancer Res; 12(12); 1779–95. ©2014 AACR</I>.</P>

Synchronized Expression of Two Bombyx mori Caspase Family Genes, ice-2 and ice-5 in Cells Induced by Ultraviolet Irradiation

Wang, Wenbing,Sun, Ying,Song, Lina,Wu, Yan,Wu, Huiling Korean Society of Sericultural Science 2008 International Journal of Industrial Entomology Vol.17 No.1

The caspase family proteins play an important role in programmed cell death (apoptosis). To date, the expression profiles of the caspase family genes in Bombyx mori (Bm) are poorly known. In this study, we examined the expression profiles of two novel Bm caspase family genes (ice-2 and ice-5), the potential change of the mitochondrial membrane and the morphology in Bm cells after stimulation of ultraviolet (UV) irradiation. The results showed the potential change of the mitochondrial membrane occurred at 5 hours after UV irradiation treatment. Analysis of fluorescent quantitative RT-PCR demonstrated that both the ice-2 and ice-5 might be involved in UV induced apoptosis in Bm cells. Notably, after UV irradiating, expression pattern of ice-2 and ice-5 were remarkably different. The ice-2 gene was highly expressed at two time points, 0.5 and 5 hours after UV stimulating, while the expression level of ice-5 only peaked at 5 hours after UV stimulating. It indicated that apoptosis induced by UV irradiation was involved in the mitochondrial pathway and the two isoforms of Bm ice may act but play different role during the apoptosis of Bm cells.