The Effects of Physicochemical Factors and Cell Density on Nitrite Transformation in a Lipid-Rich Chlorella

( Fang Liang ),( Kui Du ),( Xiaobin Wen ),( Liming Luo ),( Yahong Geng ),( Yeguang Li ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.12

To understand the effects of physicochemical factors on nitrite transformation by microalgae, a lipid-rich Chlorella with high nitrite tolerance was cultured with 8 mmol/l sodium nitrite as sole nitrogen source under different conditions. The results showed that nitrite transformation was mainly dependent on the metabolic activities of algal cells rather than oxidation of nitrite by dissolved oxygen. Light intensity, temperature, pH, NaHCO3 concentrations, and initial cell densities had significant effects on the rate of nitrite transformation. Single-factor experiments revealed that the optimum conditions for nitrite transformation were light intensity: 300 μmol/㎡/s; temperature: 30℃; pH: 7-8; NaHCO3 concentration: 2.0 g/l; and initial cell density: 0.15 g/l; and the highest nitrite transformation rate of 1.36 mmol/l/d was achieved. There was a positive correlation between nitrite transformation rate and the growth of Chlorella. The relationship between nitrite transformation rate (mg/l/d) and biomass productivity (g/l/d) could be described by the regression equation y = 61.3x (R(2) = 0.9665), meaning that 61.3 mg N element was assimilated by 1.0 g dry biomass on average, which indicated that the nitrite transformation is a process of consuming nitrite as nitrogen source by Chlorella. The results demonstrated that the Chlorella suspension was able to assimilate nitrite efficiently, which implied the feasibility of using flue gas for mass production of Chlorella without preliminary removal of NOX.

Effects of Different Ti Content in Zn-30Al Filler Metals on the Microstructure and Mechanical Properties of Mg-Al Butt Joints

Liming Liu,Fei Liu,Disheng Fang,Hongyang Wang 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

Based on the idea of alloying welding seam, a series of Zn-30Al-xTi filler metal was used to join Mg/Al dissimilar metals by tungsten inert gas welding, where the x was 0, 0.05, 0.1, 0.5, and 1, respectively. The effect of various Ti content on the microstructure and the mechanical property of Mg-Al butt joints was investigated. Results indicated that the fusion zones of the joints were mainly composed of α-Al, MgZn2 and Al-Zn eutectics when the Ti content was 0.05, 0.1 and 0.5 wt.%, and Al3Ti compounds could precipitate first and act as nucleuses during solidification process when the Ti content was appropriate. The mechanical property of the weld joints improved consistently to the increasing content of Ti. The optimum average tensile strength of the joints reached 148 MPa when Zn-30Al-0.5Ti was used, but the average tensile strength decreased again when Zn-30Al-1Ti was used.

Influence of Test Method and Poisson's Ratio Effect on Pullout Performance of Anchor Bolt in Pile

Qing Fang,Zhijun Yang,Chuanyi Zhang,Liming Yi,Xudong Fu 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.12

The influence of load pattern and Poisson’s ratio effect on the pullout performance of the anchor bolt, which were connected with pipe pile by core concrete, were investigated by three anchorage-beam pullout tests and one self-balanced pullout test. Theoretical and finite element analysis were also employed to explore the influence of load pattern and Poisson’s ratio effect on the ultimate friction at the pipe-core-concrete contact surface. The results show that the load pattern and Poisson’s ratio effect of pipe pile have a great influence on the pullout characteristics of the anchor bolt. The maximum relative displacements between core concrete and pipe pile and between anchor bolt and core concrete in self-balanced pullout test are 0.913 mm and 0.935 mm, respectively, which are significantly greater than that in anchorage-beam pullout test where the displacements are 0.292 mm and 0.500 mm, respectively. In the self-balanced test, the pipe pile is under compression and expands radially due to the Poisson’s ratio effect. In the anchorage-beam test, on the contrary, the pipe pile is under tension and shrinks radially. As a consequence, the ultimate friction between core concrete and pipe pile in the anchorage-beam test is higher than that in the self-balanced test by 15.1% to 28.7%. Accordingly, the results of anchorage-beam pullout test are more sensible for the anchor bolt design.

A flute grinding approach of end-mill based on the grid model of complex grinding wheel

Jianping Yang,Liming Wang,Jianyong Li,Yang Fang 대한기계학회 2023 대한기계학회 춘추학술대회 Vol.2023 No.11

Yeast Surface Display of Capsid Protein VP7 of Grass Carp Reovirus: Fundamental Investigation for the Development of Vaccine Against Hemorrhagic Disease

( Shaoxiang Luo ),( Liming Yan ),( Xiaohua Zhang ),( Li Yuan ),( Qin Fang ),( Yong An Zhang ),( Heping Dai ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.12

VP7, an outer capsid protein of grass carp reovirus (GCRV), was expressed and displayed on the surface of Saccharomyces cerevisiae for developing an efficient vaccine against hemorrhagic disease of grass carp. The result of flow cytometry analysis indicated that protein VP7 could be displayed on the surface of yeast cells after inducing with galactose. The expression of VP7 was confirmed by western blot analysis and further visualized with confocal microscopy. The specific antibodies against VP7 generated from mice were detectable from all immune groups except the control group, which was immunized with untransformed yeast cells. The displaying VP7 on glycosylation-deficient strain EBYΔMnn9 was detected to induce a relatively low level of specific antibody amongst the three strains. However, the antiserum of EBYΔM9-VP7 showed relative high capacity to neutralize GCRV. Further neutralization testing assays indicated that the neutralizing ability of antiserum of the EBYΔM9-VP7 group appeared concentration dependent, and could be up to 66.7% when the antiserum was diluted to 1:50. This result indicates that appropriate gene modification of glycosylation in a yeast strain has essential effect on the immunogenicity of a yeast-based vaccine.

Exothermic effects and related surface properties of the ex situ presulfurized catalysts in fabrication and activation

Yulan Gao,Xiangchen Fang,Zhenmin Cheng,Liming Xu,Zhenhui Lu,Shanda Wang 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.26 No.-

An ex-situ presulfurization technology for transforming the metal oxides of hydrotreating catalyst to the corresponding metal oxy-sulfides was developed, involving pre-dispersion of an organo-nitrogen substance onto catalyst and a subsequent multi-step heat treatment. The inherence of exothermic effect involved in the ex situ pre-sulfurized catalyst fabrication and activation, and particularly the relationship between the exothermic effect and the surface property of catalyst were investigated. The results indicated that both the preparation parameters and the operating conditions can have effects on the exothermic behavior and surface state of the catalyst. The information obtained in the current study is useful for optimizing the EPRES process, essential for hydrotreating catalyst and its application.

A Chosen-Ciphertext Secure Fuzzy Identity-Based Proxy Re-Encryption Scheme

Chunpeng Ge,Jiandong Wang,Liming Fang 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.5

Green and Ateniese introduced the notion of identity-based proxy re-encryption (IBPRE), whereby the proxy can covert a ciphertext encrypted under the delegator's identity to an encryption under the delegatee's identity of the same message. In some situations, biometric, such as dactylogram, was used as identities. However, these biometric identities will inherently have some noise when they are sampled each time. To make identity-based proxy re-encryption flexible on identities, we introduced a new primitive called fuzzy identity-based proxy re-encryption (FIB-PRE), in which an identity is viewed as a set of descriptive attributes. In a fuzzy identity-based proxy re-encryption scheme, an identity Wll can decrypt a ciphertext re-encrypted under another identity W, if and only if W and W l are close to each other as measured by the “set overlap” distance metric. In this work, we first formulate the security model of a FIB-PRE scheme. Finally, we present a construction of FIB-PRE and prove its CCA security under the decisional bilinear Diffie-Hellman (DBDH) assumption in the random model.

Research on Non-interactive Construction based on Fuzzy Conditional Proxy Re-encryption

Chunpeng Ge,Jiandong Wang,Liming Fang 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.12

In a conditional proxy re-encryption (C-PRE) scheme, a semi-trusted proxy can transform Alice’s ciphertext into Bob’s ciphertext without learning the underlying plaintext, if the ciphertext satisfies a certain condition. To achieve more fine- grained delegation on conditions, Fang, Wang and Ge introduced the notion of fuzzy conditional proxy re-encryption (FC-PRE), whereby the conditions is viewed a set of descriptive keywords. The proxy with a re-encryption key for a condition word set W can convert a ciphertext encrypted with a condition word set W1, if and only if W and W1 are close to each other as measured by the "set overlap" distance metric. Nonetheless, they left an open problem on how to construct a non-interactive FC-PRE scheme. Furthermore, their scheme is only proved secure in the random oracle model. In this paper, we answer the above problems affirmatively by presenting a non-interactive fuzzy conditional proxy reencryption scheme. Moreover, our scheme is proved secure without random oracles.

A Case of Ocular Sparganosis in China: Episode of Migration from Muscle Cone to Subconjunctiva

Xinyuan Chen,Yanyuan Fang,Liming Tao,Miao Liu,Kun Liang 대한기생충학ㆍ열대의학회 2022 The Korean Journal of Parasitology Vol.60 No.6

To improve our understanding of the migration of sparganum in humans, we report a case of ocular sparganosis having the migratory episode from the muscle cone to the subconjunctiva. A 34-year-old woman was admitted to the Hospital of Anhui Medical University (Hefei, China), in December 2019. She presented with conjunctival hemorrhage and recurrent pain in the left eye. A foreign body was found in the muscle cone of the eye. Two months later, a ribbon-like white material was found under the conjunctiva on slit-lamp examination. A long and slender, actively moving parasite was extracted by surgery. The extracted worm was approximately 8 cm long and 2 mm wide. The worm was whitish, wrinkled, ribbon shaped, and had a slightly enlarged scolex. The worm sample was morphologically identified as a plerocercoid larva (sparganum) of the Spirometra tapeworm. Her conjunctival blood suffusion and eye pain ceased within 1 week after operation. She has been in good health without any symptoms during the 2-year follow-up. A case of ocular sparganosis, in which larval worm migrated from the muscle cone to the subconjunctiva is reported from China.

Response of Esophagus to High and Low Temperatures in Patients With Achalasia

( Yutang Ren ),( Meiyun Ke ),( Xiucai Fang ),( Liming Zhu ),( Xiaohong Sun ),( Zhifeng Wang ),( Ruifeng Wang ),( Zhao Wei ),( Ping Wen ),( Haiwei Xin ),( Min Chang ) 대한소화기기능성질환·운동학회 2012 Journal of Neurogastroenterology and Motility (JNM Vol.18 No.4

Background/Aims Achalasia patients would feel exacerbated dysphagia, chest pain and regurgitation when they drink cold beverages or eat cold food. But these symptoms would relieve when they drink hot water. Reasons are unknown. Methods Twelve achalasia patients (mean age, 34 ± 10 years, F:M, 3:9) who never had any invasive therapies were chosen from Peking Union Medical College Hospital. They were asked to fill in the questionnaire on eating habits including food temperature and related symptoms and to receive high-resolution manometry examination. The exam was done in 2 separated days, at swallowing room temperature (25oC) then hot (50oC) water, and at room temperature (25oC) then cold (2oC) water, respectively. Parameters associated with esophageal motility were analyzed. Results Most patients (9/12) reported discomfort when they ate cold food. All patients reported no additional discomfort when they ate hot food. Drinking hot water was effective in 5/8 patients who ever tried to relieve chest pain attacks. On manometry, cold water increased lower esophageal sphincter (LES) resting pressure (P = 0.003), and prolonged the duration of esophageal body contraction (P = 0.002). Hot water decreased LES resting pressure and residue pressure during swallow (P = 0.008 and P = 0.002), increased LES relaxation rate (P = 0.029) and shortened the duration of esophageal body contraction (P = 0.003). Conclusions Cold water could increase LES resting pressure, prolong the contraction duration of esophageal body, and exacerbate achalasia symptoms. Hot water could reduce LES resting pressure, assist LES relaxation, shorten the contraction duration of esophageal body and relieve symptoms. Thus achalasia patients are recommended to eat hot and warm food and avoid cold food. (J Neurogastroenterol Motil 2012,18:391-398)