Growth hormone-releasing peptide-biotin conjugate stimulates myocytes differentiation through insulin-like growth factor-1 and collagen type I

( Chae Jin Lim ),( Jung Eun Jeon ),( Se Kyoo Jeong ),( Seok Jeong Yoon ),( Seon Deok Kwon ),( Jina Lim ),( Keedon Park ),( Dae Yong Kim ),( Jeong Keun Ahn ),( Bong Woo Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.9

Based on the potential beneficial effects of growth hormone releasing peptide (GHRP)-6 on muscle functions, a newly synthesized GHRP-6-biotin conjugate was tested on cultured myoblast cells. Increased expression of myogenic marker proteins was observed in GHRP-6-biotin conjugate-treated cells. Additionally, increased expression levels of insulin-like growth factor-1 and collagen type I were observed. Furthermore, GHRP-6-biotin conjugate-treated cells showed increased metabolic activity, as indicated by increased concentrations of energy metabolites, such as ATP and lactate, and increased enzymatic activity of lactate dehydrogenase and creatine kinase. Finally, binding protein analysis suggested few candidate proteins, including desmin, actin, and zinc finger protein 691 as potential targets for GHRP6-biotin conjugate action. These results suggest that the newly synthesized GHRP-6-biotin conjugate has myogenic stimulating activity through, at least in part, by stimulating collagen type I synthesis and several key proteins. Practical applications of the GHRP-6-biotin conjugate could include improving muscle condition. [BMB Reports 2015; 48(9): 501-506]

멀티미디어 : 멀티미디어 클라이언트-서버 응용 개발 도구인 한우리 / C 의 미들웨어

임채덕(Lim Chae Deok) 한국정보처리학회 1995 정보처리학회논문지 Vol.2 No.5

본 논문에서는 기존의 클라이언트-서버 응용 개발 도구에 비해서 멀티미디어 처리 기능이 강화되고, 분산 시스템 소프트웨어의 전위(Front-End) 역할을 수행 하는 멀티미디어 클라이언트-서버 응용 개발 도구(한우리/C)를 제안한다. 한우리/C는 분산 처리 환경(Distributed Computing Environment:DCE)위에서 멀티미디어 응용 개발을 지원하는 제 4세대 언어 형태의 클라이언트-서버 도구이다. 본 논문에서는 한우리/C를 구성하는 요소들 중에서 미들웨어 부분에 대한 설계와 구현된 내용을 설명한다. The Client-Server model is a computing technique that efficiently uses distributed resources via networks. But, generating an application using Client-Server model requires many different expertises to developers in comparison to that of using the centralized computing method. That is, an application developer must be familiar with network programming and GUI(Graphical User Interface) technique in addition to conventional programming skills. Accordingly, the time and man power problems have been issued for building a Client-Server system. To alleviate these problem, the Client-Server applications development tools are needed. To meet such a need, we developed a GUI based tool, called Hanuri/C, for generating Client-Server application programs. In comparison to existing Client-Server tools, Hanuri/C is reinforced Multimedia facilities. Hanuri/C is considered as a front-end part of distributed system software. That is, Hanuri/C is a Client-Server tool similar to Forth Generation Language(4GL) supporting multimedia application on top of the Distributed Computing environment(DCE). In this paper, we present the design and implementation method of the middleware part of Hanuri/C.

Automatic Arrangement Algorithm for Tower Cranes Used in High-rise Apartment Buildings

Lim, Chae-Yeon,Kim, Sun-Kuk,Seo, Deok-Seok,Son, Ki-Young The Korean Institute of Building Construction 2012 한국건축시공학회지 Vol.12 No.3

On most construction sites, the arrangement of tower cranes is decided by site engineers based on their own experience, which can cause cost overruns and delays in the lifting work. Although many researchers have conducted studies on tower crane arrangement using computer modeling and knowledge-based expert systems as well as mathematical models, no research has aimed to develop an algorithm to identify an optimum solution among several alternatives for installation areas of tower cranes satisfying the conditions of lifting work. The objective of this study is to develop an automatic arrangement algorithm for tower cranes used in high-rise apartment construction. First, as a new concept, a possible installation area of tower cranes was suggested. Second, after proposing several alternatives based on the installation points suggested in this study, an algorithm analyzing the economic feasibility of tower cranes was developed considering the rental, installation and removal costs. Third, a case study was conducted to prove the validity of the developed algorithm for selecting and installing an effective set of tower cranes at minimum cost.

Enhanced Systemic siRNA Delivery Using Reducible Bile Acid-modified PEI for Cancer Therapy

Chae eun PARK,Su yeon LIM,Da hwun KIM,Siyan LYU,Byung deok KIM,Oui bo WHANG,Ji hoon JEONG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Small interfering RNA (siRNA) contributes to RNA interference(RNAi) by inhibiting target gene expression in a sequence-specific manner. siRNA delivery system have become increasingly popular for cancer therapy. In this study, we used a low molecular weight polyethylenimine (PEI, 1.8 kDa) conjugated with deoxycholic acid (DA). A DA-PEI conjugate was further modified with 4-fluorothiophenol (4-FTP) (4-FTP-DA-PEI) to enhance systemic siRNA delivery. The thiophenol group would be involved with disulfide bonds between the polymer chains and siRNA modified with free thiols (thiol-siRNA) to form and π-π interactions between the pendent aromatic groups and coprostane ring of the bile acid. The stabilized TP-DA-PEI conjugate with thiol-siRNA achieved developed intracellular uptake, serum stability, and transfection efficiency. Furthermore, it shows high accumulation of TP-DA-PEI/thiol-siRNA polyplexes and significant tumor growth inhibition effect in tumor-bearing mice after systemic administration.

Physiological Responses of Calystegia soldanella under Drought Stress

Bae, Chae-Youn,Hwang, Jeong-Sook,Bae, Jeong-Jin,Choi, Sung-Chul,Lim, Sung-Hwan,Choi, Deok-Gyun,Kim, Jong-Guk,Choo, Yeon-Sik The Ecological Society of Korea 2013 Journal of Ecology and Environment Vol.36 No.4

This study was conducted to determine the extent of drought resistance based on physiological responses of Calystegia soldanella under water deficit. In order to investigate the changes of plant growth, stomatal density, photosynthesis, chlorophyll fluorescence, the contents of chlorophyll and carotenoid, osmolality, total ion contents, the contents of carbohydrate and proline, C. soldanella was grown under well watered and drought stressed conditions for 12 days. In this study, water-deficit resulted in remarkable growth inhibition of C. soldanella. The effect of water-deficit on plant growth was associated with low osmotic potential of soil. On day 12 after drought treatment, dry weight, relative water contents, number and area of leaves and stem length were lower than those of control. The stomatal conductance and net photosynthetic rate were significantly reduced in water stressed plant to regulate inner water contents and $CO_2$ exchange through the stomatal pore. Chlorophyll fluorescence and chlorophyll contents were not different in comparison with the control, indicating that the efficiency of photosystem II was not affected by drought stress. This results could be explained that water-deficit in C. soldanella limits the photosynthetic rate and reduces the plant's ability to convert energy to biomass. A significant increase in total ion contents and osmolality was observed on day 7 and day 12. Accumulation of proline in leaves is associated with the osmotic adjustment in C. soldanella to soil water-deficit. Consequently, this increase in osmolality in water stressed plant can be a result in the increase of ion contents and proline.

문서의 지형학적 특징 추출을 위한 수리형태학적 방법

박덕민(Deok Min Park),임채욱(Chae Wook Lim),박규태(Kyu Tae Park) 대한전자공학회 1994 대한전자공학회 학술대회 Vol.1994 No.7

Human Amniotic Fluid Stem Cell-derived Muscle Progenitor Cell Therapy for Stress Urinary Incontinence

전소영,Deok Hyun Cho,Seon Yeong Chae,Kyung Hee Choi,Hyun Ju Lim,Ghil Suk Yoon,김범수,Bup Wan Kim,James J Yoo,Tae Gyun Kwon 대한의학회 2012 Journal of Korean medical science Vol.27 No.11

The most promising treatment for stress urinary incontinence can be a cell therapy. We suggest human amniotic fluid stem cells (hAFSCs) as an alternative cell source. We established the optimum in vitro protocol for the differentiation from hAFSCs into muscle progenitors. These progenitors were transplanted into the injured urethral sphincter and their therapeutic effect was analyzed. For the development of an efficient differentiation system in vitro, we examined a commercial medium, co-culture and conditioned medium (CM) systems. After being treated with CM, hAFSCs were effectively developed into a muscle lineage. The progenitors were integrated into the host urethral sphincter and the host cell differentiation was stimulated in vivo. Urodynamic analysis showed significant increase of leak point pressure and closing pressure. Immunohistochemistry revealed the regeneration of circular muscle mass with normal appearance. Molecular analysis observed the expression of a larger number of target markers. In the immunogenicity analysis, the progenitor group had a scant CD8 lymphocyte. In tumorigenicity, the progenitors showed no teratoma formation. These results suggest that hAFSCs can effectively be differentiated into muscle progenitors in CM and that the hAFSC-derived muscle progenitors are an accessible cell source for the regeneration of injured urethral sphincter.

N termini of apPDE4 isoforms are responsible for targeting the isoforms to different cellular membranes.

Jang, Deok-Jin,Park, Soo-Won,Lee, Jin-A,Lee, Changhoon,Chae, Yeon-Su,Park, Hyungju,Kim, Min-Jeong,Choi, Sun-Lim,Lee, Nuribalhae,Kim, Hyoung,Kaang, Bong-Kiun Cold Spring Harbor Laboratory Press 2010 Learning & Memory Vol.17 No.9

<P>Phosphodiesterases (PDEs) are known to play a key role in the compartmentalization of cAMP signaling; however, the molecular mechanisms underlying intracellular localization of different PDE isoforms are not understood. In this study, we have found that each of the supershort, short, and long forms of apPDE4 showed distinct localization in the cytoplasm, plasma membrane, and both plasma membrane and presynaptic terminals, respectively. The N-terminal 20 amino acids of the long form of apPDE4 were involved in presynaptic terminal targeting by binding to several lipids. In addition, the N terminus of the short form of apPDE4 bound to several lipids including phosphoinositols, thereby targeting the plasma membrane. Overexpression of the long and the short forms, but not the supershort form attenuated 5-HT-induced membrane hyperexcitability. Finally, the knockdown of apPDE4s in sensory neurons impaired both short-term and long-term facilitation. Thus, these results suggest that apPDE4s can participate in the regulation of cAMP signaling through specific subcellular localization by means of lipid binding activities.</P>

Sequential Chemical Bath Deposition of Cu_2–<i>x</i>Se/CdS Film by Suppressing Ion-Exchange Reaction

Cai, Gangri,Lim, Iseul,Lee, Deok Yeon,Shrestha, Nabeen K.,Lee, Joong Kee,Nah, Yoon-Chae,Lee, Wonjoo,Han, Sung-Hwan American Chemical Society 2012 The Journal of physical chemistry B Vol.116 No.24

<P>Chemical bath deposition is an attractive technique to form single- and multilayered metal oxide/chalcogenide films on electrode surfaces. However, the occurrence of desorption and/or ion-exchange reaction during subsequent chemical bath deposition has so far limited preparation of multilayered metal oxide/chalcogenide films. In this paper, we report a method to prevent desorption and ion-exchange reaction of metal oxide/chalcogenide on electrode surfaces using a polyelectrolyte multilayer during sequential chemical bath deposition. By controlling the ion permeability of the polyelectrolyte multilayer, Cu<SUB>2–<I>x</I></SUB>Se film was successfully deposited on the CdS film. The Cu<SUB>2–<I>x</I></SUB>Se/CdS film is confirmed by UV–vis absorption spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis, and X-ray powder diffractometer. Furthermore, the Cu<SUB>2–<I>x</I></SUB>Se/CdS films were investigated as photoinduced charge transfer devices which showed photocurrents of 0.22 mA/cm<SUP>2</SUP> under illumination (<I>I</I> = 100 mW/cm<SUP>2</SUP>).</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpcbfk/2012/jpcbfk.2012.116.issue-24/jp301617q/production/images/medium/jp-2012-01617q_0001.gif'></P>

Mutations in the Antifolate-Resistance-Associated Genes Dihydrofolate Reductase and Dihydropteroate Synthase in Plasmodium vivax Isolates from Malaria-Endemic Countries.

Lu, Feng,Lim, Chae Seung,Nam, Deok Hwa,Kim, Kwonkee,Lin, Khin,Kim, Tong-Soo,Lee, Hyeong-Woo,Chen, Jun-Hu,Wang, Yue,Sattabongkot, Jetsumon,Han, Eun-Taek Allen Press, etc.] 2010 The American journal of tropical medicine and hygi Vol.83 No.3

<P>Parasite dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS) are known target enzymes of antifolate drugs used for the treatment and prophylaxis of persons with malaria. We sequenced the Plasmodium vivax dihydrofolate reductase (pvdhfr) and dihydropteroate synthase (pvdhps) genes to examine the prevalence and extent of point mutations in isolates from malaria-endemic countries. Double mutations (S58R and S117N) or quadruple mutations (F57L/I, S58R, T61M, and S117T) in the pvdhfr gene were found in isolates from Thailand (96.4%) and Myanmar (71.4%), but in only one isolate (1.0%) from Korea, where sulfadoxine-pyrimethamine has never been used. The pvdhfr point mutations correlated strongly with the pvdhps point mutations and ranged from single to triple mutations (S382A, A383G, and A553G), among isolates from Thailand, Myanmar, and Korea. These findings suggests that the prevalence of mutations in pvdhfr and pvdhps in P. vivax isolates from different malaria-endemic countries is associated with selection pressure imposed by sulfadoxine-pyrimethamine.</P>