Pretreatment with Danshensu Promotes Germination of Cucumber (Cucumis sativus L.) Seeds by Regulating Reactive-Oxygen Species and Endogenous Phytohormones

Zhu Lijun 한국식물학회 2021 Journal of Plant Biology Vol.64 No.3

Danshensu (DSS), the aqueous extracts of Salvia miltiorrhiza has been widely used in biomedical science due to its outstanding capacity in ROS scavenger. However, studies regarding its effects on plant physiology are still scare. In the present study, cucumber seeds were soaked in different concentrations of DSS solution for 24 h before subjected to germination to test its effects on seed germination, and further to explore the metabolic and molecular mechanisms. The results shown that 0.1% DSS had significantly promoted the germination rate, radical length, as well as soluble sugar and H2O2 contents. DSS pretreatment upregulated transcript levels of genes involved in SOD, POD, and CAT biosynthesis, concurrent with promoted enzyme activities. In accordance with lower ABA, but higher GA contents, transcript levels of genes involved in ABA and GA biosynthesis were down and upregulated, respectively, in DSS pretreated seeds. DSS pretreatment also stimulated ethylene production and related genes expression levels. These positive effects of DSS on seed germination were confirmed by addition of inhibitors of GA, ABA, NADPH oxidase, and H2O2 scavenger. In conclusion, DSS stimulated cucumber seed germination by mediating the regulation of endogenous hormones with promoted ROS levels. Our results highlight the potentiality of DSS in plant science.

Improved Kalman filter with unknown inputs based on data fusion of partial acceleration and displacement measurements

Lijun Liu,Jiajia Zhu,Ying Su,Ying Lei 국제구조공학회 2016 Smart Structures and Systems, An International Jou Vol.17 No.6

The classical Kalman filter (KF) provides a practical and efficient state estimation approach for structural identification and vibration control. However, the classical KF approach is applicable only when external inputs are assumed known. Over the years, some approaches based on Kalman filter with unknown inputs (KF-UI) have been presented. However, these approaches based solely on acceleration measurements are inherently unstable which leads poor tracking and so-called drifts in the estimated unknown inputs and structural displacement in the presence of measurement noises. Either on-line regularization schemes or post signal processing is required to treat the drifts in the identification results, which prohibits the real-time identification of joint structural state and unknown inputs. In this paper, it is aimed to extend the classical KF approach to circumvent the above limitation for real time joint estimation of structural states and the unknown inputs. Based on the scheme of the classical KF, analytical recursive solutions of an improved Kalman filter with unknown excitations (KF-UI) are derived and presented. Moreover, data fusion of partially measured displacement and acceleration responses is used to prevent in real time the so-called drifts in the estimated structural state vector and unknown external inputs. The effectiveness and performance of the proposed approach are demonstrated by some numerical examples.

Experimental study and modeling of residence time distribution in impinging stream reactor with GDB model

Lijun Ji,Kui Chen,Jiawen Zhu,Bin Wu 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.4

Residence time distribution (RTD) in an impinging streams reactor with two or four nozzles was investigated with KCl solution as a tracer. The results showed that the flow pattern in the reactor was close to that in a continuous stirred tank reactor (CSTR). Macromixing process in the reactor was improved obviously when the opposite nozzles were added. Based on the analysis of flow region in the reactor, gamma distributionmodel with bypass (GDB) was applied for study on the RTD of the reactor. It was found that RTD in the impinging streams reactor could be finely described by the model. Also the effects of experimental conditions on parameters of model were analyzed according to the correlated values of the model parameters.

Effect of surface roughness on the flotation separation of hematite from fine quartz

Zhanglei Zhu,Zhen Li,Wanzhong Yin,Bin Yang,Ningning Zhang,Jinzhou Qu,Songjiang Chen,Jing Chang,Yuexian Yu,Lijun Liu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

Although the effect of surface roughness on the floatability of a single mineral has been investigatedextensively, a systematic study for the effect of surface roughness on the flotation separation of differentminerals, e.g., flotation recovery, has never been dwelled. To fulfill this gap, in this study, the effect of surfaceroughness on the flotation separation of hematite from quartz was investigated with a series ofexperiments and detections. Micro-flotation experiments demonstrated that hematite particles withhigher roughness resulted in higher recoveries for both hematite and quartz, but the improvement ofhematite recovery was more significant, which promoted the flotation separation of hematite fromquartz with a higher separation index (SI). Adhesion force measurements revealed that after interactingwith 125 mg/L sodium oleate at pH 9, increasing nano-scaled roughness improved the hydrophobicity ofhematite surface, and reduced the adhesion force of water droplet on hematite surface. Scanning ElectronMicroscope – Energy Dispersive Spectrometer observations confirmed that increasing nano-scaled roughnessincreased the number of fine quartz particles coating on hematite surface. Combined with the analysisof adhesion of water droplet and observation of fine particles coating on hematite surface, the effectof surface roughness on minerals flotation separation could be better understood.

The Split-Permeation Grouting Mechanism of Loose and Broken Coal Rock Masses considering the Temporal and Spatial Characteristics of Slurry Viscosity

Hexuan Zhu,Lijun Han,Qingbin Meng,Jun Liu,Lingdong Meng,Wenlong Dong 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5

Quick-setting grout has time-varying characteristics, and the space porosity of the injected medium changes after being grouted. The above two points need to be considered when loose and broken coal rock masses are grouted with rapid-setting grout. In other words, the coupling effect of the slurry-coal rock mass causes the slurry to exhibit the temporal and spatial characteristics of slurry viscosity (TSCSV). Based on the Bingham fluid constitutive model, a capillary group model with a linear increase in diameter was created, and a one-dimensional penetration grout diffusion model was established considering the TSCSV. The one-dimensional visualization splitting-permeation grout diffusion simulation test system was designed to obtain the space porosity of the broken coal rock mass under different final grouting pressures to obtain the law of the changes in grouting pressure and diffusion height with time under the condition of a fixed grouting rate. Furthermore, the mechanism of splitting-permeation grouting in loose and broken coal rock masses is analysed in depth considering the TSCSV. The results showed that the space porosity of coal rock masses increases linearly. In addition, the calculated value of the slurry diffusion distance was 0.93 − 1.1 times the experimental value considering the TSCSV. The calculated value was in good agreement with the experimental value. The calculated value of the final pressure of orifice grouting was approximately 1.5 times the test value without considering the unevenness of the space porosity distribution (USPD). The grouting pressure was overestimated without considering the USPD. Therefore, the TSCSV of the quick-setting slurry should be fully considered in the grouting design.

Identification of two rare mutations c.1318G>A and c.6438+2T>G in a Chinese DMD family as genetic markers

Yingchuan Zhu,Lijun Yang,Tengjiao Ma,Yilu Lu,Dachang Tao,Yunqiang Liu,Yongxin Ma 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background Duchenne muscular dystrophy (DMD) is a fatal X-linked recessive disorder with no efective treatment, which underscores the importance of avoiding the birth of children with DMD by identifying pathogenic mutations and obtaining an accurate prenatal diagnosis. Objective The objective of this study was to analyze the genetic defect of a Chinese family where all male patients have died of DMD. Methods Multiplex ligation dependent probe analysis (MLPA) and next-generation sequencing (NGS) were employed to detect DMD mutations. The candidate mutations were then validated by Sanger sequencing. In vitro splicing assay was further conducted to examine the potential efect of the novel DMD splice site mutation on splicing. Results We found that two rare DMD mutations c.1318G>A and c.6438+2T>G passed from generation to generation among female carriers and they may be used as genetic markers in the Chinese DMD family. In vitro splicing assay further revealed that the novel classical splice site mutation c.6438+2T>G gave rise to a new donor splice site, which resulted in a frame shift of the transcripts and a premature termination at position 2159 in exon 45 (p.Y2144Nfs*16). Conclusion We found that two co-inherited mutations passed from generation to generation in female carriers and they may be used as genetic markers in the Chinese DMD family. Our fndings not only expanded the DMD mutation spectrum, but also provided an important basis for identifying of female carriers and avoiding the birth of afected male children in this DMD family.

Factors Influencing the Quality of Standardized Treatment for Patients with Post–Cardiac Arrest Syndrome

Jing Lu,Lijun Liu,Jianliang Zhu,Xinying Guo 대한심장학회 2017 Korean Circulation Journal Vol.47 No.4

Background and Objectives: For the present study, we investigated the factors that influence the quality of standardized treatment for patients with post–cardiac arrest syndrome (PCAS) to improve the quality of PCAS treatment. Subjects and Methods: We collected data on patients with cardiac arrest (CA) who were admitted to the intensive care units (ICUs) of 11 hospitals-Class II Grade A or above-in Suzhou from January to October 2013. Indexes of standardized treatment were observed within 72 hrs of CA. We analyzed monitoring techniques, monitoring frequency, ICU human and material resources, and intensivists’ knowledge of PCAS treatment to explore how those factors affected the management of patients with PCAS. Results: The bed/nurse ratio and the frequency with which core temperature was recorded correlated closely with the implementation of therapeutic hypothermia (TH) within 6 hrs of CA. The bed/doctor ratio and intensivists’ knowledge about PCAS correlated closely with high-quality blood glucose control within 6 hrs of CA. Furthermore, the frequency with which core temperature was recorded was an independent factor influencing the quality of TH implementation, and the number times blood gas was analyzed was an independent factor influencing how well partial pressure of carbon dioxide was kept within the normal range in the 6 hrs after CA. Conclusion: The frequency of core temperature measurements and the number of times blood gas is analyzed are the most important factors influencing the quality of standardized treatment for patients with PCAS.

Use of Long-chain Alcohol in Extraction and Purification of Lincomycin from Fermentation Broth

Wu, Bin,Zhu, Jiawen,Chen, Kui,Ji, Lijun,Guo, Jianguo,Zhao, Jianxi 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.2

Low selectivity for lincomycin in butanol extraction process leads to relatively higher content of impurities. A novel process for extraction of lincomycin from fermentation broth was studied in this work. Mixture of n-octanol and n-decanol is used as extractant to replace n-butanol in extraction of lincomycin. Oprimal operation conditions for the process have been studied. Due to higher extraction selectivity for lincomycin A by long-chain alcohol, content of impurity(lincomycin B) in the final product is much lower than that in product by butanol process. Furthemore, the practicability for combination of long-chain alcohol and butanol in purification of linecomycin was investigated.

Study of the Effects of Compaction and Split Grouting on the Structural Strengthening Characteristics of Weakly Cemented Argillaceous Rock Masses

Lingdong Meng,Lijun Han,Hexuan Zhu,Wenlong Dong,Wei Li 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.4

There is a wide distribution of weakly cemented argillaceous rock masses in areas where there are many coal mines, and their complex characteristics make them more difficult to support. This paper researches this type of rock and studies the slurry diffusion mechanism and grouting reinforcement characteristics of injected rock mass and their relationship with compaction and splitting grouting. The grouting mode is dominated by compaction and splitting. The rheological behavior of cement slurries with different concentrations used in split grouting was studied. The existing grouting equipment was improved, and grouting tests were performed on loose aggregates of weakly cemented primary rock in a certain size range. Subsequently, basic physical parameter determination tests, microscopic tests, and uniaxial tests were performed on grout, and the effects of grouting pressure, moisture content of standard samples, slurry water/cement ratio, and curing time on grouting reinforcement were analyzed. During the grouting process, the pattern of plasma pulse diffusion and reinforcement was summarized as three types: compaction, splitting, and microcracking. The failure modes of rock were summarized as shear failure, shear failure along the interface of the plasma, and splitting damage.

Study on the Influence of Formation Temperature on the Water Absorption and Weathering of Swelling Rock in Marine Coal Mines

Lingdong Meng,Lijun Han,Hexuan Zhu,Wenlong Dong,Wei Li 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.11

To study the mechanisms and potential control measures for coal mine engineering safety hazards caused by swelling rock, water absorption experiments were performed under different temperature by using an independently developed rock water absorption test system. The swelling pressure, expansion amount and water absorption amount of specimens were monitored in real time. Nuclear magnetic resonance and uniaxial compression experiments were performed. The water absorption rate of the swelling rock specimens increases significantly with increasing water absorption time. The water absorption capacity of the specimens differs considerably under different temperatures. The water absorption curve can be divided into three stages: the severe stage, deceleration stage and equilibrium stage. The water absorption amount and water absorption rate are linearly related to temperature. The curve of the swelling pressure depicts a double peak shape. The expansion curve can also be divided into three stages. With increasing temperature, the expansion rate increases, and the time needed to achieve expansion stability is prolonged. There is a negative linear correlation between the uniaxial compressive strength and the moisture content. The higher the temperature is, the higher the moisture content. The increase in the rock porosity after water absorption is one of the important factors driving the strength attenuation.