Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells

Zhang Ying,Wang Peng,Jin Mei-xian,Zhou Ying-qi,Ye Liang,Zhu Xiao-juan,Li Hui-fang,Zhou Ming,Li Yang,Li Shao,Liang Kang-yan,Wang Yi,Gao Yi,Pan Ming-xin,Zhou Shu-qin,Peng Qing 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.3

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs. METHODS: hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated. RESULTS: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2–related factor 2 signaling. CONCLUSION: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.

Differential Gene Expression in the Pathogenic Strains of Actinobacillus pleuropneumoniae Serotypes 1 and 3

( Fang Xie ),( Ming Jun Zhang ),( Shu Qing Li ),( Chong Tao Du ),( Chang Jiang Sun ),( Wen Yu Han ),( Liang Zhou ),( Lian Cheng Lei ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.4

Study on Temperature Drop Effect Caused by Low-Temperature Pouring of Concrete Dams

Fang Wang,Chunju Zhao,Yihong Zhou,Anran Zheng,Huawei Zhou,Zhipeng Liang,Pan Gong 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.8

Low-temperature concrete pouring on dams causes sudden temperature drops on the surfaceof the previously poured concrete, thus inducing thermal cracks. In this study, a field test and anumerical calculation were performed on the temperature drop effect caused by lowtemperaturepouring to prevent thermal cracks in concrete dams. Firstly, a small spatial-scaletemperature-gradient monitoring scheme was designed for concrete dams based on thedistributed temperature sensing (DTS) system. Secondly, a field test was conducted during theconstruction period of the Baihetan extra-high arch dam using this scheme, analyzing thetemperature response of low-temperature pouring. Finally, a numerical simulation model wasestablished on the temperature field and stress field of the dam, studying the temperaturedrop effect caused by low-temperature concrete pouring under different constructionconditions and its countermeasures. The results indicate that the temperature monitoringscheme proposed in this paper could effectively capture the temperature drop effect at asmall-spatial scale caused by low-temperature pouring. This effect, as a negative impact oncrack prevention of dam concrete, is effectively weakened using uniform surface-flowingwater cooling with a temperature of 22 − 24oC.

Transition over 35 Years in the Incidence Rates of Primary Central Nervous System Tumors in Shanghai, China and Histological Subtyping Based on a Single Center Experience Spanning 60 Years

Shen, Fang,Wu, Chun-Xiao,Yao, Yu,Peng, Peng,Qin, Zhi-Yong,Wang, Yin,Zheng, Ying,Zhou, Liang-Fu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: Only few epidemiological data on primary central nervous system (CNS) tumors in Shanghai have been reported. Methods: All cases of primary CNS tumors that were registered at Center for Disease Control and Prevention (CDC) were collected (1973-2007: urban Shanghai; 2003-2007: whole Shanghai city). Trends were analyzed using joinpoint analysis and rates were stratified by age, gender and region. Histological data were collected from both CDC and Huashan Hospital. Results: From 1973 to 2007, the five-year average incidence rate in urban Shanghai increased in both genders, especially in the elderly population. Joinpoint analysis showed the age-adjusted incidence rate for males increased first but then plateaued, whilst rates for females continued increasing over the 35 years. For the five-year status quo (2003-2007), rural had a higher age-adjusted incidence rate than urban populations, and females higher than males, especially those with advanced age. According to CDC (2003-2007) and Huashan Hospital (1951-2011), the two most common histological subtypes were neuroepithelial tumors (with male predominance) and meningiomas (with female predominance). Conclusions: In Shanghai, a steadily increased incidence rate of primary CNS tumors was observed in general, and in the elderly and female population in particular.

Feedback Design of Temperature Control Measures for Concrete Dams Based on Real-Time Temperature Monitoring and Construction Process Simulation

Huawei Zhou,Yihong Zhou,Chunju Zhao,Fang Wang,Zhipeng Liang 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.5

Crack prevention is a significant issue in the construction process of concrete dams. The vast majority of concrete cracks arerelated to temperature variations, and hence, temperature control is a primary method used to prevent cracks. This paper presents anew integrated concept, named Feedback Design (FD) of temperature control measures, which incorporates real-time temperaturemonitoring, temperature field simulation and construction process simulation in a large system to optimize a temperature controlscheme for concrete dams. We used a fiber-optic Distributed Temperature Sensing (DTS) system to monitor the temperaturevariation process of typical dam monoliths in real time. By incorporating a genetic algorithm, neural network algorithm, and finiteelement theory, we proposed an intelligent inversion method to obtain thermal parameters of concrete dams based on the temperaturemonitoring data. Meanwhile, we built a simulation model of a concrete dam’s construction process based on a cycling networktechnique to obtain accurate construction environmental parameters. Finally, we simulated the temperature field of a typical dammonolith based on initial temperature control measures, inversion thermal parameters, and accurate construction environmentalparameters. Temperature variance lines of typical points in the model obtained from the finite element method (FEM) accord wellwith the measured values obtained from the DTS system. We applied the FD approach to the Xiluodu arch dam located in southwestChina to forecast the temperature fields and optimize the temperature control scheme of the dam blocks planned for pouring. The FDapproach has been proven reliable and efficacious.

Selection and evaluation of RT-qPCR reference genes for expression analysis in the tiny egg parasitoid wasp, Trichogramma dendrolimi matsumura (Hymenoptera: Trichogrammatidae)

Huo Liang-xiao,Bai Xue-ping,Che Wu-nan,Ning Su-fang,Lv Lin,Zhang Lisheng,Zhou Jin-cheng,Dong Hui 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2

The egg parasitoid, Trichogramma spp., is an important biological control agent used against a broad range of Lepidopteran pests in agriculture and forestry. The biology of Trichogramma has been studied in details. Further studies should focus on the molecular mechanisms of Trichogramma by qualifying the expression of related genes It is critical to select appropriate reference genes for normalizing RT-qPCR results and establishing a robust method for quantifying target gene expression. This study aimed to identify and validate appropriate reference genes for use in RT-qPCR analysis of Trichogramma dendrolimi. Ten candidate housekeeping genes, namely betaactin (ACTIN), forkhead box O (FOXO), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), heat shock pro tein 90 (HSP90), ribosomal protein L10a (RPL10a), L18 (RPL18), L28 (RPL28), S13 (RPS13), S15 (RPS15), and superoxide dismutase (SOD), were tested for their suitability as reference genes for developmental stage (3rd, 4th, 5th, 6th, 7th, 8th, 9th, and 10th day after parasitization), tissue (head, thorax, and abdomen of adults), sex of adults (male and female), and temperature (17℃, 25℃, and 32℃). According to the GeNorm analysis, a robust analysis should involve using an appropriate combination of reference genes, namely, at least three genes for different development stages, two genes for different tissues, two genes for different sex, and two genes for different temperatures, respectively. According to the RelFinder method by the integrated results of GeNorm, NormFinder, BestKeeper, and the ΔCt method, we identified the developmental stage-specific reference genes SOD, GAPDH, and ACTIN; tissue-specific reference genes RPL18 and RPS15; sex-specific reference genes RPL18 and SOD; and temperature-specific reference genes RPL18 and RPL10a. This study provides a standardized procedure for the quantification of gene expression in T. dendrolimi and will be helpful for future biological control programs using Trichogramma wasps.

Temperature Field Reconstruction of Concrete Dams based on Distributed Optical Fiber Monitoring Data

Huawei Zhou,Zhiguo Pan,Zhipeng Liang,Chunju Zhao,Yihong Zhou,Fang Wang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.5

Mastering the real temperature distribution of the concrete dam is the basis for solving the problem of temperature control and crack prevention. In this paper, Distributed Temperature Sensing (DTS) technology was applied to temperature monitoring of a higharch dam under construction in southwest China. In order to obtain a more comprehensive temperature distribution of the dam, optical fiber layout principles for arch dams were studied, and horizontal and vertical optical fiber layout schemes were first proposed according to these principles. The real temperature variation processes of the dam were obtained in real time with a line temperature measurement pattern instead of a point temperature measurement pattern. Additionally, a framework of distributed optical fiber data acquisition and remote transmission was proposed. Interconnection of multiple DTS hosts and remote transmission of temperature data were realized. Then, two-dimensional temperature fields of a typical dam block in different ages and longitudinal profiles of dam blocks with embedded fibers were reconstructed based on large amounts of temperature monitoring data and the Kriging difference algorithm. Temperature field reconstruction results showed that the temperature distribution law of the concrete arch dam was in accordance with the actual situation.

Speech Intelligibility Enhancement Using Convolutive Non-negative Matrix Factorization with Noise Prior

Jian Zhou,Xianyong Fang,Liang Tao,Li Zhao 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.7

Comparative Genomic Analysis Reveals That the 20K and 38K Prophages in Listeria monocytogenes Serovar 4a Strains Lm850658 and M7 Contribute to Genetic Diversity but Not to Virulence

( Chun Fang ),( Tong Cao ),( Ying Shan ),( Ye Xia ),( Yong Ping Xin ),( Chang Yong Cheng ),( Houhui Song ),( John Bowman ),( Xiao Liang Li ),( Xiang Yang Zhou ),( Wei Huan Fang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1

Listeria monocytogenes is a foodborne pathogen of considerable genetic diversity with varying pathogenicity. Initially, we found that the strain M7 was far less pathogenic than the strain Lm850658 though both are serovar 4a strains belonging to the lineage III. Comparative genomic approaches were then attempted to decipher the genetic basis that might govern the strain-dependent pathotypes. There are 2,761 coding sequences of 100% nucleotide identity between the two strains, accounting for 95.7% of the total genes in Lm850658 and 92.7% in M7. Lm850658 contains 33 specific genes, including a novel 20K prophage whereas strain M7 has 130 specific genes, including two large prophages (38K and 44K). To examine the roles of these specific prophages in pathogenicity, the 20K and 38K prophages were deleted from their respective strains. There were virtually no differences of pathogenicity between the deletion mutants and their parent strains, although some putative virulent factors like VirB4 are present in the 20K region or holin-lysin in the 38K region. In silico PCR analysis of 29 listeria genomes show that only strain SLCC2540 has the same 18 bp integration hotspot as Lm850658, whereas the sequence identity of their 20K prophages is very low (21.3%). The 38K and 44K prophages are located in two other different hotspots and are conserved in low virulent strains M7, HCC23, and L99. In conclusion, the 20K and 38K prophages of L. monocytogenes serovar 4a strains Lm850658 and M7 are not related to virulence but contribute to genetic diversity.

Method and application of spatial positioning for valid temperature-measuring optical fibers in concrete dams

Huawei Zhou,Chunju Zhao,Zhipeng Liang,Yihong Zhou,Fang Wang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8

Spatial positioning of Valid Temperature-measuring Optical Fiber (VTOF) is of great significancefor accurately grasping the real temperature distribution of a concrete dam. However, there is barely an effective way to accurately locate the measuring points of optical fiber in concrete dams. A comprehensive data symmetry analysis technique is presented for determining the optimal spatial positioning of valid temperature-measuring optical fibers (VTOFs) in concrete dams. This approach includes a mathematical model to confirm the center of symmetry in monitoring data, which is required to reconstruct the real spatial temperature distribution. This process can identify VTOF locations and verify the reliability of temperature predictions. Experimental validation was conducted using findings from research on a new mathematical model, site measurements from a concrete dam, and corresponding data from distributed optical fibers. These results were successfully applied to the construction of the Baihetan dam; the concrete temperature from 405 pouring blocks in 5 typical dam monoliths was accurately monitored, and the temperature-measuring points of VTOFs were more than 24711. Meanwhile, the optical fiber temperature measurement and temperature field analysis system of Baihetan dam located in southwest China was developed based on accurately acquired temperature measurement data, which provides data basis and engineering application platform support for temperature evolution and temperature field analysis and precise temperature control management of pouring blocks.