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Lee, Sung-Sik,Lee, Jae-ung,Oh, Ju Hyeon,Park, Soojin,Hong, Yin,Min, Byeong Ki,Lee, Hyun Hee L.,Kim, Hugh I.,Kong, Xianglei,Lee, Sungyul,Oh, Han Bin The Royal Society of Chemistry 2018 Physical chemistry chemical physics Vol.20 No.48
<P>Chiral differentiation of protonated isoleucine (Ile) using permethylated β-cyclodextrin (perCD) in the gas-phase was studied using infrared multiple photon dissociation (IRMPD) spectroscopy, ion-mobility, and density functional theory (DFT) calculations. The gaseous protonated non-covalent complexes of perCD and d-Ile or l-Ile produced by electrospray ionization were interrogated by laser pulses in the wavenumber region of 2650 to 3800 cm<SUP>−1</SUP>. The IRMPD spectra showed remarkably different IR spectral features for the d-Ile or l-Ile and perCD non-covalent complexes. However, drift-tube ion-mobility experiments provided only a small difference in their collision cross-sections, and thus a limited separation of the d- and l-Ile complexes. DFT calculations revealed that the chiral distinction of the d- and l-complexes by IRMPD spectroscopy resulted from local interactions of the protonated Ile with perCD. Furthermore, the theoretical results showed that the IR absorption spectra of higher energy conformers (by ∼13.7 kcal mol<SUP>−1</SUP>) matched best with the experimentally observed IRMPD spectra. These conformers are speculated to be formed from kinetic-trapping of the solution-phase conformers. This study demonstrated that IRMPD spectroscopy provides an excellent platform for differentiating the subtle chiral difference of a small amino acid in a cyclodextrin-complexation environment; however, drift-tube ion-mobility did not have sufficient resolution to distinguish the chiral difference.</P>
Dong Soo Park,Soo Kwon Park,Bong Chun Lee,Song Yi Song,Nam Soo Jun,Norvie L. Manigbas,Jun Hyun Cho,Min Hee Nam,Jong Seong Jeon,Chang Deok Han,Kyung Jin Choi,Doh Hoon Kim,Young Min W 한국유전학회 2009 Genes & Genomics Vol.31 No.4
The rice embryo is rich in lipid and protein bodies, bioactive chemicals such as dietary fiber, phytic acids, vitamin B and E, and gamma aminobutyric acid (GABA) than the endosperm. In this paper, we report a new giant embryo mutant, ge(t), induced from somaclonal variation derived by anther culture in rice. Sequence analysis of Cytochrome P450 of the ge(t) mutant revealed that ge(t) is a new allele of the GE gene with a single point mutation with substitution of amino acid, W395 to L395. The weight of the ge(t) mutant embryo was 3.7 times higher than normal embryo. Tocopherol and mineral content were also higher than the previously reported giant embryo rice variety, Keunnun. These results indicated that this new giant embryo rice (ge(t)) offers a promising source of genetic material in improving nutritional quality of rice especially tocopherol, essential minerals, and GABA.
Lee, Hyun Hee L.,Heo, Chae Eun,Seo, Nari,Yun, Seung Gyu,An, Hyun Joo,Kim, Hugh I. American Chemical Society 2018 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.140 No.48
<P>Practical applications of innovative host-guest systems are challenging because of unexpected guest competitors and/or subtle environmental differences. Herein, a supramolecular mass spectrometry (MS)-based method using a synthetic host, cucurbit[7]uril (CB[7]), was developed for identifying and quantifying <I>N</I>-glycolylneuraminic acid (Neu5Gc) in therapeutic glycoproteins, which critically reduces drug efficacy. The development of a reliable derivatization-free analytical method for Neu5Gc is highly challenging because of the interference by the abundant <I>N</I>-acetylneuraminic acid (Neu5Ac). CB[7] recognized the subtle structural differences between Neu5Gc and Neu5Ac. Distinct host-guest interactions between CB[7] and the two sialic acids produced a highly linear relationship between the complexation and concentration proportions of the two sialic acids in MS. Furthermore, the developed method had sub-picomolar quantification limits and a wide range of applicability for diverse glycoproteins, demonstrating the potential utility of this method as a reliable assay of Neu5Gc in therapeutic glycoproteins.</P> [FIG OMISSION]</BR>
땅콩과 땅콩새싹 추출물의 resveratrol과 aspartic acid 함량분석 및 지방세포분화 억제효능
이현희(Hyun Hee L. Lee),최상윤(Sang Yoon Choi) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.4
Interest in peanuts driven by their various biological activities and abundant nutrition engenders research interest in peanut sprouts for determining their biological activities and nutrition. Several research groups have recently studied the peanut sprout ingredients, but these studies focused on the peanut sprout extracted by methanol, an inedible extraction solvent. Thus, the present study provides the contents of two biologically active compounds, resveratrol and aspartic acid, in peanut and peanut sprout extracted by hot water and fermented ethanol, two edible extraction solvents, using UPLC-MS/MS technique. The UPLC-MS/MS results show that the peanut sprout extracted by fermented ethanol has the highest resveratrol and aspartic acid contents. This extract also exhibited the highest inhibitory effect on adipocyte differentiation, which the resveratrol may induce. Consequently, peanut sprout extracted by fermented ethanol might be helpful for the development of functional food plant materials. 식품산업 활용성을 고려하여 땅콩과 땅콩새싹의 열수와 주정 추출물을 제조하고 추출물내 resveratrol과 aspartic acid 함량을 UPLC-MS/MS를 이용하여 분석한 결과 땅콩새싹의 주정추출물이 가장 높은 resveratrol과 aspartic acid 함량을 나타내었다. 또한 땅콩새싹의 주정 추출물은 3T3-L1 세포에 처리시지방세포분화를 유의적으로 억제하였다. 따라서 땅콩새싹의주정추출물은 기능성식품소재로 활용 가능성이 높을 것으로 판단된다. 향후 이러한 결과를 바탕으로 땅콩의 발아조건 및 추출시간과 온도에 따른 활성성분 함량을 측정하여 최적의 활성성분 추출조건을 확립하는 연구를 진행할 계획이다.
무와 무청의 활성 성분 함량 및 지방세포분화 억제 효과 비교
이현희(Hyun Hee L. Lee),허진영(Jinyoung Hur),최상윤(Sang Yoon Choi) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.4
본 연구에서는 무와 무청에 함유된 활성 물질인 glucoraphenin과 ferulic acid의 함량을 UPLC TQ-MS system을 이용하여 정량 분석하였다. 정량 결과 무청 주정 추출물이 11,971.8 μg/g의 glucoraphenin과 851.7 μg/g의 ferulic acid를 함유하고 있어 무의 주정 추출물과 무와 무청의 물 추출물보다 높은 함량을 나타내었다. 3T3-L1 세포를 이용한 지방세포분화 억제 시험에서도 무청 주정 추출물이 100 ppm 농도에서 세포독성 없이 유의적인 지방세포분화 억제효과를 보였고, 이는 무청 물 추출물이나 무 추출물보다 가장 높게 나타났다. 따라서 무청 주정 추출물은 향후 활성 성분을 다량 함유한 기능성 식품소재로서 활용될 가능성이 높을 것으로 사료된다. Radish (Raphanus sativus) has been reported to exert antioxidant, anti-microbial, anti-coagulant, anti-hyperlipidemic, and anti-hypertensive effects. However, there have been no reports so far on the comparison of active ingredients and adipogenesis inhibitory activity between various parts of the radish. In this study, we analyzed the glucoraphenin and ferulic acid content in radish root and leaf extracts using a UPLC TQ-MS system. Also, their adipogenesis inhibitory effects were examined using 3T3-L1 preadipocytes. The results of this study showed that radish leaf extract has higher glucoraphenin (11,971.8 μg/g in EtOH extract) and ferulic acid (851.7 μg/g in EtOH extract) content than radish root extracts and the adipocyte differentiation suppressing activity was also higher than the radish root extracts. These results indicate that the radish leaf is rich in active ingredients and has a higher inhibitory effect on adipocyte differentiation than the radish root. Therefore, radish leaves could be used in the active compound extraction and development of functional material for adipogenesis suppression.