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참개구리(Rana nigromaculata)의 난자형성 단계에 따른 Vitellogenin의 선택적 이동
이양림,고경희 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-
참개구리의 난모세포에서 난자형성단계에 따른 vitellogenin(VTG)의 이동이 난모세포막에 의하여 조절될 것이란 생각에 기초하여 단계에 따른 VTG 이동량의 변화와 난모세포막의 변경이 VTG 이동에 미치는 영향을 조사하였다. VTG의 이동은 난자형성과정중 특히 초기난모세포에서 가장 활발하였고, 중기 및 말기에는 감소하는 경향을 나타내었다. 반면, bovine serum albumin은 모든 단계의 난모세포에서 이동을 보이지 않았다. 이러한 결과로 보아 난자형성과정중 난모세포내로의 VTG 이동은 단계특이적이고 선택적임을 알 수 있었다. 난모세포막의 막단백질을 trypsin으로 제거시키거나 막단백질의 당잔기를 wheat germ agglutinin(WGA)으로 포화시키든가 혹은 endoglycosidase F로 당잔기를 제거시킨 결과 VTG의 이동이 급격히 억제되었다. 이와 같은 결과들은 난모세포의 막단백질이 VTG 이동에서 수용체로 작용한다는 사실을 강하게 암시하였다. Changes in transport of ^125I-labeled vitellogenin into the oocytes during oogenesis were studied in connection with the stage-specificity of the transport and correlation of the transport to the functional differentiation of the oocyte membrane. Vitellogenin isolated from estrogen-treated females was found to be transported most actively into the oocytes of specific protein, was not incorporated into the oocytes of any size class. The stage=specific transport of vitellogenin appears to be controlled by membrane proteins, since the incorporation was greatly reduced when the proteins were digested by trypsin. Carbohydrate residues of the membrane proteins appear to play a very significant role in transferring vitellogenin into oocytes, since the transport of vitellogenin was blocked by saturating with wheat germ agglutinin or by removing with endoglycosidase F. The observations suggest that the selective transport of vitellogenin into the oocytes of specific stages during oogenesis is mediated probably by vitellogenin receptors, which are proteins in chemical nature.
Feasibility of single‐port retroperitoneoscopic adrenalectomy in dogs
Ko, Jonghyeok,Jeong, Junemoe,Lee, Sungin,Son, Hyunglak,Kweon, Oh‐,Kyeong,Kim, Wan Hee John Wiley and Sons Inc. 2018 Veterinary surgery Vol.47 No.suppl1
<P><B>Abstract</B></P><P><B>Objective</B></P><P>To evaluate the feasibility of single‐port retroperitoneoscopic adrenalectomy (SPRA) in dogs.</P><P><B>Study Design</B></P><P>A pilot experimental study.</P><P><B>Animals</B></P><P>Eight healthy beagle dogs.</P><P><B>Methods</B></P><P>SPRA was performed on the left and right sides (4 dogs each). Resection of the adrenal gland was performed through a SILS port using a retroperitoneal approach. Operative time was defined from skin incision to the completion of skin suture. Postoperative pain was evaluated by using 3 pain scores. Integrity of the adrenal gland capsule was evaluated by histologic assessment.</P><P><B>Results</B></P><P>Mean time taken to complete the SPRA was 44.1 minutes (range, 37‐51) and was significantly longer on the right side than on the left side (<I>P</I> < .05). There were no complications intraoperatively or during 14 days of postoperative monitoring. The adrenal gland capsule was found to be injured in 3 of the 8 dogs by histologic assessment.</P><P><B>Conclusion</B></P><P>This is the first report of SPRA in the veterinary literature. With this technique it is possible to perform adrenalectomy with some risk of capsule penetration and with excellent visibility.</P><P><B>Clinical significance</B></P><P>This study suggests that SPRA is feasible and can be used to resect small adrenal tumors with minimal complications.</P>
Avian malaria associated with Plasmodium spp. infection in a penguin in Jeju Island
Ko, Kyeong-Nam,Kang, Sang-Chul,Jung, Ji-Youl,Bae, Jong-Hee,Kim, Jae-Hoon The Korean Society of Veterinary Science 2008 大韓獸醫學會誌 Vol.48 No.2
Plasmodium spp. in domestic and wild birds are microscopic, intracellular parasitic protozoa within the blood cells and tissues cause avian malaria. A 17-month-old Magellan penguin (Spheniscus magellanicus) with a clinical signs of anorexia, depression, and respiratory distress for 3 days was submitted to the Pathology Department of Veterinary Medicine, Cheju National University in October 2005. It was born and reared in the Jeju Island. Grossly, the liver was enlarged, pale and friable. The spleen was also enlarged with dark red coloration and friable. Histopathologically, the lesions in the liver were characterized by multifocal infiltration of macrophages and lymphocytes especially in perivascular regions. The schizonts of Plasmodium spp. contained up to 30 merozoites were found in numerous infiltrated mononuclear cells. Similarly, histiocytic cells were proliferated in red pulp of spleen and the schizonts were found in these cells. Numerous dark brown pigments were widely distributed in the liver and spleen. The result of the nested polymerase chain reaction clarified the causative agent of this case was Plasmodium spp.. This is the first report for the outbreak of avian malaria caused by Plasmodium spp. in a penguin that was born and reared in Jeju Island in Korea.
Kyeong Ok Choi,Seung Cheol Yang,Dong Eun Kim,Wie-Soo Kang,Malshick Shin,Yoon-Hee Choi,Sanghoon Ko 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.6
The surfaces of different-sized endosperm, bran, and husk rice powders were modified using carboxymethylation. Carboxymethylation was carried out using aqueous alkalization and neutralization. After the carboxymethylation process, the centrifuged products were milled and classified by size: particles passed through sieves of 45, 106, and 300-㎛ width. The effect of carboxymethylation on physical properties such as solubility and dispersibility of endosperm, bran, and husk particles were studied. Overall, carboxymethylation increased solubility of the particles, while size reduction increased dispersibility. In particular, carboxymethylation created good aqueous suspensions by minimizing interparticle agglomeration. Our results show that the combination of size reduction and carboxymethylation improves solubility and dispersibility, resulting in better stability of the suspension. This study may be helpful for expanding the use of rice and its byproducts as ingredients in a variety of food and beverage applications.