뱀장어 아기미 울혈증에 관한 연구

김영길(Young Gill Kim),최민순(Min Soon Choi),박성우(Sung Woo Park),이근광(Keun Kwang Lee) 한국어병학회 1994 한국어병학회지 Vol.7 No.2

In order to elucidate the outbreak mechanisms of a new disease which is characterized by an intense congestion in central venus sinuses(CVS) of gill filaments in cultured eel. these experiments were carried out; epidemically surveyed on the cultured eel farms in the vicinity of Kunsan city and experimentaliy outbreaked the disease in the stressful condition such as thermal and handling shock and innoculated the supernatant from the homogenate of naturally severe congested gill into eels and onto the monolayer of the CHSE-214. Although the frequency of congestion in eels of B, C, D and E farms were higher than in eels of A farms, the water qualities (stocked and cultured water) among farms were not a great difference. In eels of B, C, D and E farms, the values of haematocrit (Ht), haemoglobin (Hb), total protein (Tp), albumin (Alb), glucose (Glu), magnesium (Mg) were lower and the values of calcium(Ca), methemoglobin(Met-Hb), glutamic pyruvic transminase (GPT), glutamic oxalacetic transminase (GOT) higher than in eels of A farms. These valules have not related to the frequency of congestion. An intensive congestion and dilataton in CVS of gill filaments in experimentally handling-stressed eels produced similar histopathological changes to those observed in the spontaneously diseased eel, but not in eels experimentally injected with filtering contents. The cytopathic effect on the CHSE-214 was not observed. In stressed eels the congestion of gill was increased in relation to either the decrease ranges of water temperature or the incerase in accllimated times. And increase in Ht, Met-Hb, Alb, Glu, GOT and GPT and decrease in Mg, Hb and Tp were found, which had a close relationship to congestion of gill. Cortisol were increased according to the decrease ranges in acclimated water temperature. From these results, decrease in water temperature during selection placed eels upon the stressed condition, made increase in ionic strength in blood stream, and CVS was dilatated owing to the increased blood inflow.

Effects of Silk Fibroin on Oxygen radicals and Their Scavenger Enzymes in Brain of SD Rats

Lee,Jong Soo,Kim,Dae Ik,Kim,Jeung Min,Park,Soo Hyun,Choi,Jin Ho,Cho,Weon Ki,Lee,Kwang Gill,Yeo,Joo Hong,Lee,Yong Woo 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.29 No.-

This study was designed to investigate the effects of silk fibroin (Mw 500) powder (SFP) on oxygen radicals and their scavenger enzymes in brain membranes of rats. Sprague-Dawley (SD) male rats (160±10g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0 g/㎏ BW/day for 6 weeks. Hydroxyl radical (·OH) levels resulted in a considerable decreases (6.6% and 9.7%, 2.8% and 11.9%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group, but were significantly decreased in these membranes of SFP-5.0 group only. Superoxide radical (0₂￣) levels were a slightly decreased (2.0% and 9.1%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. Lipid peroxide (LPO) levels were significantly decreased (12.9% and 21.9%, 13.2% and 22.5%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (16.7% and 15.7%, respectively) in brain microsomes of SFP-2.5 and SFP-5.0 groups compared with control group, but significant difference between in brain mitochondria of these two groups could not be obtained. Mn-SOD activities were remarkably increased (11.2% and 24.2%, respectively) in mitochondria of SFP-2.5 and SFP-5.0 groups. Cu,Zn-SOD activities were effectively increased (7.7% and 19.6%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups, but significant difference between control and SFP-2.5 groups could be not obtained. GSHPx activities were considerably increased (5.3% and 11.7%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. These results suggest that anti-aging effect of silk fibroin may play an effective learning and memory role in a attenuating a oxidative stress and increasing a scavenger enzyme activity in brain membranes.

The Improvement of Learning and Memory Ability of Normal Persons by BF-7

Lee, Sang-Hyung,Kim, Yong-Sik,Kang, Yong-Koo,Kwon, Oh-Sang,Shin, Yong-Kyoo,Song, Jln-Ho,Lee, Moo-Yeol,Lee, Kwang-Gill,Yeo, Joo-Hong,Lee, Won-Bok,Lee, Tae-Jin,Kim, Sung-Su The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.6

To investigate whether BF-7, extracted from Bombyx mori, improved learning and memory of ordinary people, Rey-Kim Memory Test was performed with placebo group (32 persons), 200 mg BF-7 treated group (33 persons) and 400 mg BF-7 treated group (34 persons). BF-7 enhanced significantly learning and memory function in a dose-dependent manner. To know how BF-T plays such a positive role, we measured the concentration of acetylcholine in the brain from memory impairment animal model. Treatment of BF-7 significantly increased the concentration of acetylcholine. So, it is supposed that the protection of cholinergic neuron and/or keeping proper concentration of acetylcholine might be one of the mechanisms by which BF-7 improve brain function. So, our results suggest that the BF-7 is effective material for improving learning and memory ability.

Silk Fibroin Microsphere and Its Characterization

( Kwang Gill Lee ),( Joo Hong Yeo ),( Yong Woo Lee ),( Hae Yong Kweon ),( Soon Ok Woo ) 한국잠사학회 2003 International Journal of Industrial Entomology Vol.6 No.2

Effects of Silk Fibroin on Oxygen Radicals and Their Scavenger Enzymes in Liver of SD Rats

Lee,Jong Soo,Kim,Dae Ik,Kim,Jeung Min,Park,Soo Hyun,Choi,Jin Ho,Lee,Kwang Gill,Yeo,Joo Hong,Lee,Yong Woo 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.29 No.-

This study was designed to investigate the effects of silk fibroin (Mw 500) powder (SFP) on oxygen radicals and their scavenger enzymes in liver membranes of rats. Sprague-Dawley (SD) male rats (160±1Og) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0g/㎏ BW/day for 6 weeks. Hydroxyl radical (·OH) levels resulted in a considerable decreases (5.8% and 8.4%, 3.7% and 11.1%, respectively) in liver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group, and O₂￣ radical level was remakably decreased about 15% and 20% in liver cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. Lipid peroxide (LPO) levels were significantly decreased (8.3% and 18.0%, 13.4% and 18.4%, respectively) in liver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were remarkably decreased about 19.0% and 24.4% in liver microsomes of SFP-2.5 and SFP-5.0 groups, but significantly decreased about 11.6% in liver mitochondria of SFP-5.0 group compared with control group. Mn-SOD activities were remarkably increased (17.6% and 28.8%, respectively) in mitochondria of SFP-2.5 and SFP-5.0 groups, and Cu,Zn-SOD activities were also effectively increased (6.0% and 14.4%, respectively) in liver cytosol of SFP-2.5 and SFP-5.0 groups, but significant difference between GSHPx activity in liver cytosol of these two groups could be not obtained. These results suggest that anti-aging effect of silk fibroin may play an effective anti-aging role in a attenuating a oxidative stress and increasing a scavenger enzyme activity in liver membranes.

Synthesis and characterization of phosphorescent iridium complexes of 6-chloro-3-phenylpyridazine and 3-chloro-6-(3^′-methoxy-phenyl)-4-methyl-pyridazine

Lee, Sang Jin,Sung Lee, Jae,Hwang, Kwang-Jin,Kim, Young Kwan,Kim, Young Sik,Park, Noh Gill,Shin, Eun Ju,Lee, Seung Hee Elsevier 2005 CURRENT APPLIED PHYSICS Vol.5 No.1

<P><B>Abstract</B></P><P>The preparation and photophysics of organometallic Ir(III) complexes with 3-phenylpyridazine (H6Clppdz) and its derivative are reported. IrCl<SUB>3</SUB>·<I>n</I>H<SUB>2</SUB>O reacts with H6Clppdz, and 3-chloro-6-(3<SUP>′</SUP>-methoxyphenyl)-4-methyl-pyridazine (MCOMppdz) to give the corresponding chloride-bridged dimers, (6Clppdz)<SUB>2</SUB>Ir(<I>μ</I>-Cl)<SUB>2</SUB>Ir(6Clppdz)<SUB>2</SUB> and (MCOMppdz)<SUB>2</SUB>Ir(<I>μ</I>-Cl)<SUB>2</SUB>Ir(MCOMppdz)<SUB>2</SUB> in good yields. These chloride-bridged dimers are cleaved with 2-pyrazinecarboxylic acid (Hpyzc), 2-picolinic acid (Hpic), acetylacetone (Hacac), and 2,2,6,6-tetramethyl-3,5-heptanedione (Htmd) to give the corresponding monomer, (6Clppdz)<SUB>2</SUB>Ir(pyzc), (6Clppdz)<SUB>2</SUB>Ir(pic), (6Clppdz)<SUB>2</SUB>Ir(acac), (6Clppdz)<SUB>2</SUB>Ir(tmd), (MCOMppdz)<SUB>2</SUB>Ir(acac), and (MCOMppdz)<SUB>2</SUB>Ir(tmd), respectively. The complexes show bright orange to red luminescence at room temperature and the emission wavelenghts are affected by the ancillary ligands as well as cyclometalating ligands (593–664 nm).</P>

Effects of Silk Fibroin on Oxidative Stress and Membrane Fluidity in Brain of SD Rats

Lee,Jong Soo,Kim,Dae Ik,Kim,Jeung Min,Park,Soo Hyun,Choi,Jin Ho,Lee,Kwang Gill,Yeo,Joo Hong,Lee,Yong Woo 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.29 No.-

This study was designed to investigate the effects of silk fibroin powder(Mw 500) on oxidative stress and membrane fluidity in brain membranes of rats. Sprague-Dawley (SD) male rats (160±10g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0g/㎏ BW/day for 6 weeks. Cholesterol level was significantly decreased about 8.0% in brain microsomes of SFP-5.0 group only compared with control group. Membrane fluidities were significantly increased (12.9% and 15.2%, respectively) in brain microsomes of SFP-2.5 and SFP-5.0 groups, but significant difference between in brain mitochondria of these two groups could be not obtained. Basal oxygen radicals (BOR) in brain mitochondria and microsomes were significantly inhibited (10.4% and 24.0%, 7.9% and 14.9%, respectively) by SFP-2.5 and SFP-5.0 groups compared with control group. Induced oxygen radicals (IOR) in brain mitochondria and microsomes were significantly inhibited (11.8% and 14.1%, respectively) by SFP-5.0 groups compared with control group compared with control group. Lipid peroxide (LPO) levels were dose-dependently decreased (12.9% and 21.9%, 13.2% and 22.5%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (15.7% and 17.1%, 16.7% and 15.7%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. These results suggest that administration of SFP may play an effective role in a attenuating a oxidative stress and increasing a membrane fluidity in brain membranes.

작잠 실크 피브로인에 의한 in vitro 상처 회복 효과 및 에탄올 처리에 따른 작잠 실크 피브로인 스폰지의 구조 전이

이광길 ( Kwang Gill Lee ),조유영 ( You Young Jo ),여주홍 ( Joo Hong Yeo ),이희삼 ( Heui Sam Lee ),김기영 ( Kee Young Kim ),김현복 ( Hyun Bok Kim ),김안숙 ( An Sook Kim ),김성곤 ( Seong Gon Kim ),권해용 ( Haeyong Kweon ) 한국잠사학회 2014 한국잠사곤충학회지 Vol.52 No.1

작잠 누에고치를 정련한 후 질산칼슘4수화물의 용융액을 사용하여 재생 작잠 실크피브로인 스펀지를 제조하였다. 작잠 실크피브로인은 280 nm에서 tyrosine 잔기 등에 기인한 흡광대를 나타내었다. 작잠 실크피브로인 스펀지를 에탄올 농도별로 처리한 후 구조 전이를 관찰한 결과 80%에탄올 처리시에는 β-sheet 구조(700 cm-1), α-helix 구조 (625 cm-1), 그리고 random coil (660 cm-1) 구조가 공존하는 것으로 나타났다. 또한 작잠 실크피브로인을 이용한 in vitro 상처회복실험 결과 실크피브로인의 첨가에 의하여 상처회복 효과가 인정되었다. Regenerated Antheraea pernyi silk sponge was prepared using calcium nitrate 4 hydrate melt and examined the conformational changes treated with aqueous ethanol solution. The conformation of silk sponges was changed from random coil structure to β-sheet and α-helix conformation with low ethanol concentration (50 ~ 70%). On the other hand, that of silk sponges with 80% ethanol treatment showed β-sheet (700 cm?1), α-helix (625 cm?1), and random coil (660 cm?1) specific peaks. Wound healing effect in vitro was observed by cytoslective wound healing kit. Therefore, regenerated Antheraea pernyi silk sponges might be used as promising wound dressing materials.

<논문(論文)> : 천잠 누에고치의 일반 성상

이광길 ( Kwang Gill Lee ),정다은 ( Da Eun Chung ),김기영 ( Kee Young Kim ),조유영 ( You Young Jo ),김현복 ( Hyun Bok Kim ),김성국 ( Sung Kuk Kim ),권해용 ( Haeyong Kweon ) 한국잠사학회 2015 한국잠사곤충학회지 Vol.53 No.1

국내에서 생산한 천잠 누에고치의 일반 특성을 고찰한 결과 국내산 천잠 누에고치는 연두색 고치를 지으며, 층상구조를 가지고 있었다. 천잠 누에고치는 외피는 연두색이었고 내피는 흰색을 나타내어 천잠 누에고치의 색상을 나타내는 색소 성분은 외피에 존재하였다. 천잠 누에고치는 고치층의 무게는 0.528 g, 견층두께는 0.424 mm로 측정되었다. 천잠 누에고치를 구성하는 주요 아미노산은 알라닌, 글리신, 세린, 아스파르트산, 티로산, 아르기닌 순으로 나타났으며, X-선 회절분석 결과 2θ = 16.8°, 20.4° 부근에서 강한 회절 피크와 2θ = 15.0°, 24.3°, 30.0° 부근에서 날카로운 회절 피크를 나타내었다. 천잠 누에고치는 폭이 20 μm 정도인 섬유가 적층된 구조를 가지고 있으며, 고치의 안쪽과 바깥쪽에 흰색 결정을 가지고 있었다. 천잠 누에고치의 최대 열분해 온도는 370℃ 부근이었다. 이러한 천잠 누에고치에 대한 연구 결과는 향후 천잠 누에 고치를 이용한 소재 개발의 기초 자료로 활용될 수 있을 것으로 기대한다. Antheraea yamamai silkworm cocoon is considered as a natural biocompatible materials, but can not be used as medical resources due to its difficult processability. In this study, we examined the general characteristics including cocoon shell weight and structural and thermal properties of Antheraea yamamai silkworm cocoon. The cocoon shell weight and thickness of wild silkworm cocoon was 0.528 g and 0.424 mm, respectively. The cocoon has yellow-green color in outside cocoon but white in inner layer cocoon. Amino acid analysis showed that the main amino acid of Antheraea yamamai cocoon is alanine, glycine, serine, aspartic acid, tyrosine and arginine. X-ray diffractometry showed that strong diffraction peaks at 2θ = 16.8°, 20.4°, corresponded to β-sheet structure and sharp diffraction peaks at 2θ = 15.0°, 24.3°, 30.0° due to the presence of calcium oxalate on cocoon surface. Antheraea yamamai cocoon was degraded at 370℃ These results might be used as basic information for development of non-textile materials using Anthereae yamamai silkworm cocoon.

Calcium Nitrate/에탄올 혼합 용액에 의한 작잠견사(繭絲)의 용해

이광길 ( Kwang Gill Lee ),조유영 ( You Young Jo ),여주홍 ( Joo Hong Yeo ),이희삼 ( Heui Sam Lee ),김기영 ( Kee Young Kim ),박광영 ( Kwang Young Park ),권해용 ( Hae Yong Kweon ) 한국잠사학회 2013 한국잠사곤충학회지 Vol.51 No.1

Antheraea pernyi silkworm is one of typical wild silkworms, which spins a tawny color cocoon. The cocoon has been used as a resource for textile material due to strong chemical stability and good mechanical properties. In this study, to increase the solubility efficiency of A. pernyi silk fibroin, the composition of dissolution solvent were examined. Calcium chloride tertiary system, normally used for dissolution of Bombyx mori silk fibroin, does not act on A. pernyi silk fibroin. Calcium nitrate system dissolves A. pernyi silk fibroin, and calcium nitrate ethanol system do more easily than calcium nitrate system. Amino acid composition of A. pernyi silk fibroin obtained after dissolution is mainly composed of alanine, glycine, and serine. A. pernyi silk fibroin would be used for non-textile applications near future.