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고무라아유코 ( Komura Ayuko ),호소다마사히로 ( Hosoda Masahiro ) 한국유통경영학회(구 한국유통정보학회) 2017 유통경영학회지 Vol.20 No.4
This study explores ways to define fixed customers, identify the benefits earned from them, and develop relationships with them. We conducted a case study at a mail-order company and a department store. Data were collected through structured questionnaire-based interviews and analyzed by applying the KJ (Kawakita Jiro) method to categorize how interview respondents defined fixed customers, assessed their financial contribution, and managed relationships with them. The results show, first, that both companies use the total number of transactions, amount spent per transaction, purchases of core products, and the variety of products purchased by their customers to define fixed customers. Second, both companies consider sales stability to be the primary financial benefit of fixed customers. Third, both companies run a loyalty program to develop long-term relationships with fixed customers and gain financial benefits. Our study contributes to the literature in several ways. First, it suggests that the number of transactions may be amore useful classification standard than the amount spent per transaction. Second, we argue that the stability emanating from trade with fixed customers increases the forecasting accuracy of shipment numbers.
Development of Ganglioside Probes for Live Imaging of Lipid Rafts
Naoko Komura,Hiromune Ando,Kenichi Suzuki,Rahul Chadda,Yasuhiro Ikeda,Hideharu Ishida,Akihiro Kusumi,Makoto Kiso 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Lipid rafts have been assumed to work as a platform where protein receptors, cholesterols and gangliosides (representatively GM3 and GM1) assemble and interact for efficient signal transduction. According to the observation of these raft molecules by single molecule tracking technique, raft components were frequently but very transiently recruited to the cluster of GPI-anchored proteins formed upon binding of extracellular signaling molecules [1]. However, behaviors and functions of gangliosides in living cell membranes have never been extensively investigated because of the lack of their fluorescent probes that behave as equivalents of native gangliosides. To address this issue, we intend to develope novel fluorescent GM3 and GM1 probes for single molecule tracking, in which glycan parts are site-specifically labeled with various fluorescent dyes and evaluate the functionality of the fluorescent gangliosides. We designed the replacement of the C9 hydroxyl groups of sialic acid of GM3 and GM1 with amino groups to introduce fluorescent dyes. The synthesis of glycan parts of GM3 and GM1 were successfully achieved by using a Neu-Gal unit having a trifluoroacetamide at the C9 position. Next, the glycan parts were glycosidated with the Glc-Cer acceptor developed by our research group [2], yielding the skeletons of GM3 and GM1, respectively, which was followed by the conversion of trifluoroacetamide groups into amino groups. Finally the amino GM3 and GM1 were conjugated with fluorescent dyes through amide linkages, producing the targeted fluorescent gangliosides GM3 and GM1. The synthesized gangliosides were subjected to biophysical evaluations; DRM analysis and single molecule observation of colocalization with raft molecules (GPI-anchored protein, CD59 and epidermal growth factor receptor). Results obtained in these evaluations demonstrated great influence of the loaded position and polarity of fluorescent dye on the raftphilicity of gangliosides. Furthermore, the Kd value of the fluorescent GM1 probe for cholera toxin B subunit (CTXB) was comparable to that of native GM1, indicating that dye did not interfere the binding to CTXB. These results strongly suggest that the fluorescent gangliosides can be used to identify behaviors and functions of gangliosides in raft domains.
Fabrication of ZnO Hexagonal Micropyramids by Using an rf-Magnetron Sputtering Method
Shingo Komura,DaeGwi Kim,Shuji Wakaiki,Masaaki Nakayama 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.1
We report on fabrication of self-assembled ZnO hexagonal micropyramids grown on a (0001) Al2O3 substrate by using an rf-magnetron sputtering method from the aspects of thier structural and optical properties. We found that the average size of the hexagonal micropyramids increases with increasing growth temperature. The re ection and the photoluminescence spectra at 10 K demonstrate high-quality optical properties for the total growth layer. Especially, for the growth temperatures of 650 ℃ and 700 ℃, fine structures are observable in the excitonic transitions in the re ection spectra. Furthermore, under high-density excitation conditions, photoluminescence bands originating from bi-exciton formation and from an inelastic scattering process of excitons, the so-called P emission, were observed. We report on fabrication of self-assembled ZnO hexagonal micropyramids grown on a (0001) Al2O3 substrate by using an rf-magnetron sputtering method from the aspects of thier structural and optical properties. We found that the average size of the hexagonal micropyramids increases with increasing growth temperature. The re ection and the photoluminescence spectra at 10 K demonstrate high-quality optical properties for the total growth layer. Especially, for the growth temperatures of 650 ℃ and 700 ℃, fine structures are observable in the excitonic transitions in the re ection spectra. Furthermore, under high-density excitation conditions, photoluminescence bands originating from bi-exciton formation and from an inelastic scattering process of excitons, the so-called P emission, were observed.
Takai, Tomoaki,Inamoto, Teruo,Komura, Kazumasa,Yoshikawa, Yuki,Uchimoto, Taizo,Saito, Kenkichi,Tanda, Naoki,Kouno, Junko,Minami, Koichiro,Uehara, Hirofumi,Takahara, Kiyoshi,Hirano, Hajime,Nomi, Hayahi Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6
Background: Despite widely adopted standard methods for follow-up including cystoscopy plus cytology, recurrence rates of non muscle-invasive bladder cancer (NMIBC) have not improved over the past decades, still ranging from 60% through 70%. Hence, widely acceptable surveillance strategies with excellent sensitivity are needed. Early recurrence has led to the development of a novel cystoscopy technique utilizing photodynamic diagnosis (PDD). Although, no studies have evaluated the efficacy of PDD for patients of MIBC, BCG failure or 2nd-transurethelial resection (TUR). Materials and Methods: The present study was performed from October 2012 through May 2013. IRB approved 25 patients initially underwent a cystoscopy examination of white light and blue light followed by the resection of tumors identified. Resections were performed from bladder mucosa areas considered suspicious at PDD, along with PDD negative normal bladder mucosa area resected by random biopsy. Specimens were divided into two groups, PDD positive and negative. Primary endpoints were sensitivity and specificity. Results: A total of 147 specimens extracted from 25 patients were included in the analysis. Some 45 out of 92 PDD-positive specimens were confirmed to have bladder cancer, and 51 out of PDD-negative 55 specimens were confirmed to be cancer negative. The sensitivity of PDD was 91.8% (45/49) and specificity was 52.0% (51/98). The sensitivity:specificity was 89.5% (17/19) : 47.6% (30/63) in 12 2nd-TUR patients, 90.5% (19/21) : 61.1% (11/18) in seven MIBC patients, and 95.0% (19/20) : 48.5% (16/33) in eight failed BCG cases. Conclusions: PDD-TURBT has high sensitivity to diagnose BC even for 2nd-TUR, MIBC or BCG failure cases.
Synthesis of SSEA-1, SSEA-3 and SSEA-4 for developing bio-compatible-nanomaterials
Rita Pal,Naoko Komura,Akihiro Imamura,Hiromune Ando,Hideharu Ishida,Makoto Kiso 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Stage-specific embryonic antigens1 (SSEA’s) have been identified as glycosphingolipids which play diverse roles in embryonic development, such as regulation of cell growth, recognition and differentiation. It’s well-documented that human ES and iPS cell express predominantly glycosphingolipids of the globo- series2 such as SSEA-3 and SSEA-4, which are also hallmarks of human embryonal carcinoma cells. While, SSEA-1 represents a marker for murine pluripotent stem cells, in which it plays an important role in adhesion and migration of the cells in the preimplantation embryo. We are intended to integrate their biological functions into biocompatible nanomaterials for developing ES and iPS cell culturing system. To conjugate with nanomaterials these molecules have been suitably functionalized at the terminal position. In the present study, a convergent chemical synthesis of SSEA-1 SSEA-3 and SSEA-4 will be discussed.
Takai, Tomoaki,Inamoto, Teruo,Komura, Kazumasa,Tsujino, Takuya,Matsunaga, Tomohisa,Yoshikawa, Yuki,Uchimoto, Taizo,Saito, Kenkichi,Tanda, Naoki,Minami, Koichiro,Uehara, Hirofumi,Ibuki, Naokazu,Takahar Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3
Background: We vigorously reviewed patients' operation record who had adhesion of the Denonvilliers' fascia and found out most of these patients had prostatic bleeding after prostatic gland biopsies. We examined the magnitude of prostatic bleeding and frequency after biopsies and the relationship with oncological outcomes. Materials and Methods: A total of 285 patients were selected for the final analyses. Inclusion criteria were as follows: receiving MRI three weeks after biopsiesand laparoscopic radical prostatectomy within 300 days after biopsy. We divided the patients into two groups with (group A) or without (group B) prostatic bleeding. We examined the magnitude of prostatic bleeding after biopsies and the relationship with operation time (OT), positive surgical margin (PSM), biochemical recurrence (BCR) and other factors. Furthermore, we created a logistic-regression model to derive a propensity score for prostatic bleeding after biopsies, which included all patient and hospital characteristics as well as selected interaction terms, and we examined the relationship with PSM and BCR. Results: In all patients, the OT in the group B was shorter than the group A (p < 0.001). Prostatic bleeding was associated with PSM (p=0.000) and BCR (p=0.036). In this propensity-matched cohort, 11 of 116 patients in the group B had PSM as compared with 36 of 116 patients from group A (match-adjusted odds ratio, 4.30; 95%CI confidence interval, 2.06 to 8.96; P=0.000). In addition, eight of 116 patients in group B encountered BCR, as compared with 18 of 116 patients in group A (match-adjusted odds ratio, 2.48; 95%CI, 1.03 to 5.96; P=0.042). Kaplan-Meier analysis in the propensity matching cohort showed a significant biochemical recurrence-free survival advantage for being free of prostate bleeding after biopsies. Conclusions: Our findings in the present cohort should help equip surgeons to pay attention to careful excision especially for those who experienced deferred prostatic bleeding.