Effect of needle type on intravascular injection in transforaminal epidural injection: a meta-analysis

Kim, Jae Yun,Kim, Soo Nyoung,Park, Chulmin,Lim, Ho Young,Kim, Jae Hun The Korean Pain Society 2019 The Korean Journal of Pain Vol.32 No.1

Background: Lumbosacral transforaminal epidural injection (TFEI) is an effective treatment for spinal disease. However, TFEI may have several types of complications, some of which can be attributed to intravascular injection. We reviewed studies to compare the intravascular injection rate among different needle types. Methods: We searched the literature for articles on the intravascular injection rate among different needle types used in TFEI. The search was performed using PubMed, MEDLINE, the Cochrane Library, EMBASE, and Web of Science. Results: A total of six studies comprising 2359 patients were identified. Compared with the Quincke needle, the Whitacre needle reduced the intravascular injection rate (OR = 0.57, 95% CI = [0.44-0.73], P < 0.001). However, compared with the Quincke needle, the Chiba needle did not reduce the intravascular injection rate (OR = 0.80, 95% CI = [0.44-1.45], P = 0.46). In one study, the intravascular injection rate using a blunt-tip needle was lower than that using a sharp needle. In another study, the Whitacre and the blunt-tip needle have similar intravascular injection rates, while, the catheter-extension needle showed a reduced intravascular injection rate. Conclusions: This meta-analysis showed that the Whitacre needle reduced the intravascular injection rate as compared with the Quincke needle, but failed to establish that the Chiba needle can decrease the intravascular injection rate in TFEI. Moreover, the blunt-tip needle can reduce the intravascular injection rate compared with the Quincke needle, and the catheter-extension needle can reduce the intravascular injection rate compared with the Whitacre and the blunt-tip needle.

A natural compound, aristoyagonine, is identified as a potent bromodomain inhibitor by mid-throughput screening

Kim, Young Hun,Kim, Minsung,Yoo, Miyoun,Kim, Ji Eun,Lee, Heung Kyoung,Heo, Jung-Nyoung,Lee, Chong Ock,Yoo, Minjin,Jung, Kwan-Young,Yun, Chang-Soo,Moon, Sung Woong,Chang, Hye Kyung,Chung, Chul-Woong,Py Elsevier 2018 Biochemical and biophysical research communication Vol.503 No.2

<P><B>Abstract</B></P> <P>Bromodomain-containing protein 4 (Brd4) is known to play a key role in tumorigenesis. It binds acetylated histones to regulate the expression of numerous genes. Because of the importance of brd4 in tumorigenesis, much research has been undertaken to develop brd4 inhibitors with therapeutic potential. As a result, various scaffolds for bromodomain inhibitors have been identified. To discover new scaffolds, we performed mid-throughput screening using two different enzyme assays, alpha-screen and ELISA. We found a novel bromodomain inhibitor with a unique scaffold, aristoyagonine. This natural compound showed inhibitory activity <I>in vitro</I> and tumor growth inhibition in a Ty82-xenograft mouse model. In addition, we tested Brd4 inhibitors in gastric cancer cell lines, and found that aristoyagonine exerted cytotoxicity not only in I-BET-762-sensitive cancer cells, but also in I-BET-762-resistant cancer cells. This is the first paper to describe a natural compound as a Brd4 bromodomain inhibitor.</P>

HCC : PO-07 ; The comparison of radiologic findings and histopathology for the diagnosis of hepatocellular carcinoma

( Jin Nyoung Kim ),( Jae Young Jang ),( Soung Won Jeong ),( Jin Woo Choo ),( Soon Ha Kwon ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background/Aim: Detection and characterization of focal lesions in the liver is critical for screening patients with liver cirrhosis. The aim of this study was to investigate the sensitivity of magnetic resonance imaging (MRI) and spiral computed tomography (CT) for the diagnosis of hepatocellular carcinoma (HCC) and relation between radiologic findings and histopathology in HCC. Methods: One hundred four consecutive patients with HCC diagnosed by liver biopsy or hepatectomy were included. Radiologic findings such as CT and MRI were compared with histopathology. Typical radiologic finding of HCC was defined as early enhancement and early wash-out. Tumor cell differentiation was evaluated using Edmondson-Steiner criteria in liver cores. Results: Thirty one HCCs (29.8%) were grade 1 and 2, seventy three HCCs (70.9%) were grade 3 and 4. The sensitivity for detection of HCC was 83.7% for CT alone, 92.9% for MRI alone, 87.9% for both CT and MRI together, and 96% for either CT or MRI. Typical radiologic finding of HCC on CT has a tendency of well differentiated HCC (p=0.060), and typical findings of HCC on CT and MRI showed a significantly increased well differentiated HCC (p=0.013). Clinical variables were no significantly different according to the histopathology. Conclusion: Typical early enhancement and early wash-out pattern on CT and MRI showed a significantly increased well differentiated hepatocellular carcinoma on histopathology.

LC, Acute : O-057 ; The comparison of HVPG, MELD-Na, and Child-Pugh score for the prediction of mortality in patients with liver cirrhosis

( Jin Nyoung Kim ),( Jae Young Jang ),( Soung Won Jeong ),( Jin Woo Choo ),( Soon Ha Kwon ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background/Aim: Measurement and assessment of portal hypertension are very important in the caring of patients with liver cirrhosis and in predicting relevant prognosis. This study compared the prognostic values of hepatic venous pressure gradient (HVPG), model for end-stage liver disease (MELD)- Na, and Child-Pugh score for the prediction of mortality in patients with liver cirrhosis. Methods: We investigated 136 patients with liver cirrhosis who underwent HVPG between July 2009 and March 2012. Clinical variables including laboratory data, presence of ascites, HVPG, MELD-Na, and Child-Pugh score were collected and analyzed. Cox regression model was developed to identify predictors of mortality. Accuracy of prediction was analyzed using the area under the receiver’s operating characteristics curve (AUROC). Results: HVPG, MELD-Na, Child-Pugh score, hemoglobin, HDL, albumin, and PIVKA2 significantly predicted overall mortality (univariate analysis, p<0.05). MELD-Na was an independent prognostic factor for prediction of overall mortality (multivariate HR; 23.569; p=0.043). Additionally, ascites was an independent prognostic factor for 6 month mortality (multivariate HR; 5.160; p<0.001), MELD-Na and ascites were independent prognostic factors for 12 month mortality (multivariate HR; 1.845, 1.974; p=0.030, p=0.016). AUROC was 0.698 for HVPG, 0.705 for MELD-Na, 0.591 for Child-Pugh score, respectively (p=0.015, p=0.012, p=0.266). Conclusion: MELD-Na is superior than HVPG and CTP score for the prediction of mortality in patients with liver cirrhosis.

LC, Acute : O-057 ; The comparison of HVPG, MELD-Na, and Child-Pugh score for the prediction of mortality in patients with liver cirrhosis

( Jin Nyoung Kim ),( Jae Young Jang ),( Soung Won Jeong ),( Jin Woo Choo ),( Soon Ha Kwon ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background/Aim: Measurement and assessment of portal hypertension are very important in the caring of patients with liver cirrhosis and in predicting relevant prognosis. This study compared the prognostic values of hepatic venous pressure gradient (HVPG), model for end-stage liver disease (MELD)- Na, and Child-Pugh score for the prediction of mortality in patients with liver cirrhosis. Methods: We investigated 136 patients with liver cirrhosis who underwent HVPG between July 2009 and March 2012. Clinical variables including laboratory data, presence of ascites, HVPG, MELD-Na, and Child-Pugh score were collected and analyzed. Cox regression model was developed to identify predictors of mortality. Accuracy of prediction was analyzed using the area under the receiver`s operating characteristics curve (AUROC). Results: HVPG, MELD-Na, Child-Pugh score, hemoglobin, HDL, albumin, and PIVKA2 significantly predicted overall mortality (univariate analysis, p<0.05). MELD-Na was an independent prognostic factor for prediction of overall mortality (multivariate HR; 23.569; p=0.043). Additionally, ascites was an independent prognostic factor for 6 month mortality (multivariate HR; 5.160; p<0.001), MELD-Na and ascites were independent prognostic factors for 12 month mortality (multivariate HR; 1.845, 1.974; p=0.030, p=0.016). AUROC was 0.698 for HVPG, 0.705 for MELD-Na, 0.591 for Child-Pugh score, respectively (p=0.015, p=0.012, p=0.266). Conclusion: MELD-Na is superior than HVPG and CTP score for the prediction of mortality in patients with liver cirrhosis.

HCC : PO-07 ; The comparison of radiologic findings and histopathology for the diagnosis of hepatocellular carcinoma

( Jin Nyoung Kim ),( Jae Young Jang ),( Soung Won Jeong ),( Jin Woo Choo ),( Soon Ha Kwon ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background/Aim: Detection and characterization of focal lesions in the liver is critical for screening patients with liver cirrhosis. The aim of this study was to investigate the sensitivity of magnetic resonance imaging (MRI) and spiral computed tomography (CT) for the diagnosis of hepatocellular carcinoma (HCC) and relation between radiologic findings and histopathology in HCC. Methods: One hundred four consecutive patients with HCC diagnosed by liver biopsy or hepatectomy were included. Radiologic findings such as CT and MRI were compared with histopathology. Typical radiologic finding of HCC was defined as early enhancement and early wash-out. Tumor cell differentiation was evaluated using Edmondson-Steiner criteria in liver cores. Results: Thirty one HCCs (29.8%) were grade 1 and 2, seventy three HCCs (70.9%) were grade 3 and 4. The sensitivity for detection of HCC was 83.7% for CT alone, 92.9% for MRI alone, 87.9% for both CT and MRI together, and 96% for either CT or MRI. Typical radiologic finding of HCC on CT has a tendency of well differentiated HCC (p=0.060), and typical findings of HCC on CT and MRI showed a significantly increased well differentiated HCC (p=0.013). Clinical variables were no significantly different according to the histopathology. Conclusion: Typical early enhancement and early wash-out pattern on CT and MRI showed a significantly increased well differentiated hepatocellular carcinoma on histopathology.

두유 커드를 생산하는 김치 유래 젖산균의 동정

Ro-Ui Kim(김로의),Soon-Cheol Ahn(안순철),Sun-Nyoung Yu(유선녕),Kwang-Youn Kim(김광연),Jong-Hwan Seong(성종환),Young-Guen Lee(이영근),Han-Soo Kim(김한수),Dong-Seob Kim(김동섭) 한국생명과학회 2011 생명과학회지 Vol.21 No.2

본 연구의 목적은 두유 curd를 형성하는 미생물을 분리하는 것이다. 두유 curd를 형성하는 미생물은 채소를 젖산균으로 발효시킨 전통적인 한국의 음식, 김치로부터 분리하였다. 분리 균주 196개 중 10개의 균주(strain No. 2-2-2, 2-15-2, 2-18-1, 2-19-2, 3-4-1, 3-4-2, 3-8-1, 3-8-3, 3-17-1, 4-39-5)가 단단한 두유 curd를 형성하였고 분자생물학적?생화학적 분석법에 의해 동정되었다. 분리균주로부터 추출한 genomic DNA는 16S rDNA 지역의 PCR 증폭을 위한 주형으로 사용하였다. GenBank 데이터로 16S rDNA 염기서열을 비교한 결과, 분리 균주들은 Leuconostoc mesenteroides group과 Lactobacillus sakei group으로 동정되었다. 두유 curd를 형성하는 균주들의 계통 발생학적 위치와 분류군은 neighbor-joining 방법을 이용하여 확인하였다. 또한, L. mesenteroides group은 생화학적 특성에 의해 L. mesenteroides subsp. dextranicum으로 동정되었다. 하지만 L. sakei group은 생화학적 특성 비교시 다양성을 보여 Lactobacillus sp.로 명명하였다. The purpose of this study was to isolate soy curd forming bacterial strains. Soy curd forming bacteria were isolated from Kimchi, a traditional Korean vegetable food that is fermented using lactic acid bacteria. Among 196 bacterial strains, ten isolates (strain No. 2-2-2, 2-15-2, 2-18-1, 2-19-2, 3-4-1, 3-4-2, 3-8-1, 3-8-3, 3-17-1, 4-39-5) formed firm soy curd. The isolated bacterial strains were identified by molecular biological and biochemical analyses. The genomic DNAs extracted from the isolated bacterial strains were used as a template for PCR amplification of 16S rDNA region. By comparing the results of the 16s rDNA sequences with GenBank data, the isolated strains were identified as Leuconostoc mesenteroides group and Lactobacillus sakei group. The phylogenetic position of soy curd forming strains and their related taxa were investigated using neighbor-joining method. L. mesenteroides group was further identified as L. mesenteroides subsp. dextranicum based on biochemical properties. L. sakei group was named Lactobacillus sp., because it showed a variety of biochemical properties.

Effect of needle type on intravascular injection in transforaminal epidural injection: a meta-analysis

Jae Yun Kim,Soo Nyoung Kim,Chulmin Park,Ho Young Lim,Jae Hun Kim 대한통증학회 2019 The Korean Journal of Pain Vol.32 No.1

Background: Lumbosacral transforaminal epidural injection (TFEI) is an effective treatment for spinal disease. However, TFEI may have several types of complications, some of which can be attributed to intravascular injection. We reviewed studies to compare the intravascular injection rate among different needle types. Methods: We searched the literature for articles on the intravascular injection rate among different needle types used in TFEI. The search was performed using PubMed, MEDLINE, the Cochrane Library, EMBASE, and Web of Science. Results: A total of six studies comprising 2359 patients were identified. Compared with the Quincke needle, the Whitacre needle reduced the intravascular injection rate (OR = 0.57, 95% CI = [0.44–0.73], P < 0.001). However, compared with the Quincke needle, the Chiba needle did not reduce the intravascular injection rate (OR = 0.80, 95% CI = [0.44–1.45], P = 0.46). In one study, the intravascular injection rate using a blunt-tip needle was lower than that using a sharp needle. In another study, the Whitacre and the blunt-tip needle have similar intravascular injection rates, while, the catheter-extension needle showed a reduced intravascular injection rate. Conclusions: This meta-analysis showed that the Whitacre needle reduced the intravascular injection rate as compared with the Quincke needle, but failed to establish that the Chiba needle can decrease the intravascular injection rate in TFEI. Moreover, the blunt-tip needle can reduce the intravascular injection rate compared with the Quincke needle, and the catheter-extension needle can reduce the intravascular injection rate compared with the Whitacre and the blunt-tip needle.

HCC : PE-058 ; The predictive factors for the response of transarterial chemoembolization on contrast-enhanced ultrasonography

( Soung Won Jeong ),( Jae Young Jang ),( Jin Woo Choo ),( Jin Nyoung Kim ),( Soon Ha Kwon ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background/Aim: Hepatocellular carcinoma (HCC) response after transarterial chemoembolization (TACE) depends on tumor vascularization and characterization, which may be reflected in the pathognomonic pattern of HCC on contrastenhanced ultrasonography (CEUS). We assessed predictive factors on CEUS for the response of TACE. Methods: Seventeen patients with 18 HCC underwent TACE. All of the tumors were studied with CEUS before TACE. A second-generation echo-enhancer (Sono Vue; Bracco, Milan, Italy) and a low mechanical index technique were used. After TACE, tumor response was classified with a score between 1 and 4 according to the percentage of remaining enhancing tumor portion (score 1; remained enhancing lesion <25%, score 2; 25%≤enhancing lesion<50%, score 3; 50%≤enhancing lesion <75%, score 4; enhancing lesion≥75%). Score 1 was defined as “good response” after TACE. Tumor size, initial arterial enhancing time, arterial enhancing duration, intensity of arterial enhancement, presence of hypoenhanced pattern, and feeding artery to the tumor were analyzed. Results: The median size of tumor was 3.1 cm (range 1 - 14 cm). The median time of initial arterial enhancing time and arterial enhancing duration were 21 sec, and 27.5 sec, respectively. Six tumors showed strong arterial enhancement. The presence of hypoenhanced pattern were shown in 14 tumors. Fifteen tumors had feeding arteries. The number of tumor response score after TACE in all tumors were 11 tumors in score 1, 4 in score 2, 2 in score 3, and 1 in score 4. In predicting good response after TACE, initial arterial enhancing time, arterial enhancing duration, intensity of arterial enhancement, and hypoenhanced pattern did not show any significance. Presence of a feeding artery and tumor size (<5cm) were predictive factors for good response (p=0.043, p=0.047). Conclusions: The presence of a feeding artery and tumor size less than 5cm were the predictive factors for the good response of TACE on CEUS.

HCC : PE-068 ; A relationship between HCV RNA viral load and clinical outcome in patients with hepatocellular carcinoma

( Jin Woo Choo ),( Soung Won Jeong ),( Jae Young Jang ),( Jin Nyoung Kim ),( Soon Ha Kwon ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background/Aim: The HCV RNA viral load is the predictive factor for sustained viral response in the treatment of chronic hepatitis C. However, it has been reported that HCV RNA viral load is not predictive of eventual outcome of chronic hepatitis C. The relation between HCV RNA viral load and clinical outcome of hepatocellular carcinoma (HCC) is not elucidated, yet. We investigated the relation of HCV RNA viral load to the clinical outcome in HCC. Methods: Among 453 HCC patients, 34 consecutive patients diagnosed with chronic hepatitis C virus infection were classified into two groups by HCV RNA viral load. Group I (n=19) was defined as low HCV RNA viral load less than 2x106 copies/ml, and Group II (n=15) defined as high HCV RNA viral load over 2x106 copies/ml. Characteristics of HCC, complications of cirrhosis, and overall survival were compared between the two groups. Results: Sixteen patients were male (47.1%) and the median age was 69 years. There was no significant difference in age, gender, Child-Pugh score, MELD score, tumor stage, portal vein invasion, distant metastasis, α-fetoprotein, and histology between the two groups. For complications of cirrhosis includeing variceal hemorrhage, ascites, hepatorenal syndrome and hepatic encephalopathy during follow up, there was no significant difference between both groups. Median overall survival was 48.7 months (95% CI, 30.9-66.6) for group I and 54.0 months (95% CI, 36.1-71.9) for group II. (p=0.859). Conclusion: HCV RNA viral load did not affect the clinical outcome in patients with hepatocellular carcinoma.