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Driver Fatigue Evaluation Methods for Commercial Vehicles
Sung Pill Jo,Jae Hee Park,Inseok Lee,Seung Hee Kim,Min Uk Kim,Jung Tae Kim 대한인간공학회 2013 대한인간공학회 학술대회논문집 Vol.2013 No.5
Objective: 본 연구는 운전자 피로에 대한 평가 척도를 문헌 조사를 통해 알아보고, 조사된 평가 척도들 중 일부를 실차 실험을 통해 그 타당성을 알아보는데 그 목적이 있다. Background: 운전자 피로는 교통사고의 주된 원인 중 하나이다. 그러나 운전자 피로도를 평가하는 방법은 명확히 제시된 바가 없다. 지금까지의 연구 결과들을 종합할 때 운전자 피로도는 주관적 피로평가, 작업수행도 측정평가, 생리학적 측정 등 세 가지 방법으로 평가될 수 있다. 주관적 평가는 신체불편도, 피로도, 졸림감 등이 있고, 작업수행도로는 반응시간, tracking performance, 운전에러 등이 있고, 생리학적 측정방법으로는 EMG, 체압분포, 심박수 등이 주로 이용되었다. Method: 6 명의 피실험자들이 각각 약 6 시간의 고속국도 운전을 하는 동안에, 다양한 피로평가 척도를 측정해 분석하였다. Results: 실험결과 주관적 피로도 평가치, 체압분포, EMG, 요추 기립근의 MVC 등이 운전자 피로 현상과 관련이 있는 것으로 나타났다. Conclusion: 다양한 피로평가 척도를 사용해 실험을 수행한 결과, 운전자의 육체적 피로와 관련이 있는 것들로 체압분포 비의 변화 등 일부 지표만이 타당성 있는 있는 것으로 나타났다. Application: 본 연구 결과는 운전자의 피로도와 시트 평가에 활용될 수 있을 것이다.
Design, Construction and Performance of A Removable Soil Nailing System
Kim, Hong-Taek,Ryu, Jeong-Soo,Jung, Sung-Pill,Kang, In-Kyu,Park, Si-Sam 한국지반공학회 2000 지반 : 한국지반공학회지 Vol.16 No.3
쏘일네일 벽체는 국낸에서 과거 10년간 가시설 및 영구구조물에 상당수 시공되어 왔다. 그러나 쏘일네일링 공법은 많은 장점이 있음에도 불구하고 인접한 건물의 하부에 쏘일네일이 삽입되는 것을 허용하지 않아 제한적으로 사용되고 있는 실정이다. 따라서 본 논문에서는 쏘일네일이 인접한 건물의 하부에 삽입되는 문제를 해결하고 철근을 재활용할 수 있는 방안으로 제거식 쏘일네일링 공법을 소개하였다. 제서식 쏘일네일링 공법은 연직굴착시 가시설 흙막이벽체로 주로 사용할 수 있다. 제거식 쏘일네일의 인발특성을 규명하기 위해 총 23회의 현장인발실험을 실시하였으며, 이를 통해 고정자 소켓의 설치간격과 시멘트 그라우트의 W/C 비가 중요한 설계인자라는 것을 알 수 있었다. 또한 본 논문에서는 국내에서 처음으로 시공되는 제거식 쏘일네일 벽체의 설계 및 시공과 시공상태 등을 소개하였으며, 제거식 쏘일네일의 철근을 제거하기 전과 후에 벽체의 변형을 계측자료로부터 분석하였다.
Condylar Hyperplasia with Long-standing Temporomandibular Joint Dislocation
Kim, Il-Kyu,Cho, Hyun-Young,Jung, Bum-Sang,Pae, Sang-Pill,Cho, Hyun-Woo,Seo, Ji-Hoon Korean Association of Maxillofacial Plastic and Re 2014 Maxillofacial Plastic Reconstructive Surgery Vol.36 No.1
Mandibular condylar hyperplasia is an uncommon condition of excessive unilateral growth of the condyle causing facial asymmetry and occlusal alterations. The etiology of condylar hyperplasia is unclear, but several factors are suspected, including previous trauma, hormonal disturbances, and abnormal functional loadings. Acute or chronic recurrent dislocation of temporomandibular joint (TMJ) is common, but long-standing dislocation is rare. We present two cases of the exophytic condylar hyperplasia that lasted for over 20 years with TMJ dislocation. In both cases, we performed a condyloplasty to restore normal occlusion and facial symmetry, with satisfactory results.
Derivation of Induced Pluripotent Stem Cell–Like Cells from Jeju Black Cattle Cells
Sang-Gi Jeong,Seung-Eun Lee,Yun-Gwi Park,Yeo-Jin Son,Eun-Young Kim,Se-Pill Park 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Jeju Black Cattle (JBC, endangered native Korean cattle), a species characterized by their pitch-black coat color, live in Jeju Special Self-Governing Province. Pluripotent stem cells (PSCs) derived from farm animals have potential for various applications in transgenic breeding, basic research and agriculture. However, there were a few reports for establishment of bovine induced PSC (iPSC) and their culture conditions were not optimized yet. In this study, JBC bull #5293 (date of birth, October 31, 2013) ear cells were transfected by retroviral vectors that include 5 pluripotent reprogramming factors, hOCT4, hSOX2, hKLF4, hc-MYC and hNANOG. The JBC cells were exposed to retrovirus in suspended state in a fibroblast culture medium (10% FBS+DMEM) for 3 h and then plated on culture dish (3×104 cells/60mm-dish). After three days, infected cells were dissociated and replated to a new feeder-free culture dish or mouse embryonic fibroblast (MEF) feeder cell dish (3×104 cells/60 mm-dish). And these cells were cultured in two different ES media (15% FBS+DMEM; 15% FBS or 20% SR+DMEM/F12; 20% SR) commonly supplemented with 5 ng/mL bFGF and 1,000 U LIF. Produced iPSC-like colony was separated by mechanical dissociation and transferred to a new MEF cell dish. In the result, cell aggregation before colony formation was predominantly appeared in 20% SR group compared with 15% FBS group, while the JBC cell growth was faster in 15% FBS group. In comparison of feeder-dependent or -free culture, differentiation was more frequently appeared in feeder-free condition. However, a iPSC-like colony was recovered in 15% FBS group under feeder-free culture. These iPSC-like colonies were maintained up to 3 passages. When we checked the pluripotent cell marker alkaline phosphatase (AP), the activity was strongly detected in 2nd passage JBC iPS-like cells. Further experiments were needed to find the better stable culture condition able to more passages culture (>15) for establishment of JBC-iPSCs.
Magnetic Properties of Mn-Doped ZnO Films Prepared by Reactive Cosputtering
Sang Yoon Park,이영백,Jie Hoon Kang,Pill Jin Kim,김태희,Young Jun Yoo 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.3
Zn1¡xMnxO ¯lms with smooth surfaces were prepared Si(100) substrates by reactive cosputtering of high-purity Mn and ZnO targets in an oxygen ambient with partial pressures ranging from 2.2 £ 10¡7 to 1.2 £ 10¡5 Torr. The ¯lm deposited at an oxygen partial pressure of 2.2 £ 10¡7 Torr exhibited a ferromagnetic behavior with a Curie temperature above 350 K while the ¯lms for oxygen partial pressures higher than 1.2 £ 10¡6 Torr revealed nonmagnetic properties. Our results evidently show that the oxygen vacancy plays an important role in the magnetic ordering of Mn-doped ZnO ¯lm. Our experimental results show the possibility for applications of Mn-doped ZnO-based tunneling devices.
Pioglitazone Improves Oocyte Maturation and Subsequent Developmental Competence of Porcine Embryos
Sang-Gi Jeong,Seung-Eun Lee,Won-Jae Kim,Jae-Wook Yoon,Chan-Oh Park,Hyo-Jin Park,Dong-Yong Hong,Eun-Young Kim,Se-Pill Park 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
The optimization of culture condition is both basic and important for improving oocyte maturation, subsequent development of preimplantation embryo, and further implantation. This study performed to confirm effect of pioglitazone (PIO) treatment directly on porcine oocyte during in vitro maturation, further development of embryo. Immature oocytes were cultured in IVM medium, containing 0, 0.01, 0.1, 1, and 10 μM pioglitazone (0, 0.01, 0.1, 1, and 10 PIO, respectively) for 44 h. Although both oocyte survival and polar body emission did not significantly differ between the control and PIO-treated groups, percentage of normal spindle formation and chromosome alignment was increased in the 1 PIO-treated group than in the control group (control, 60.9±8.2%; and 1 PIO, 81.5±5.9%, p<0.1). Level of GSH was significantly increased in 1 PIO-treated group than in the control group (control, 100.0±5.0%; and 1 PIO, 119.8±5.2%, p<0.05), while the level of ROS was not differ in all groups. The rate of blastocyst was significantly higher in the 1 PIO-treated group than in the control at day 6 (control, 29.6±2.5%; and 1 PIO, 37.9±2.3%, p<0.05) and 7 (control, 35.1±1.6%; and 1 PIO, 41.8±2.7%, p<0.05), respectively. These results indicated that PIO treatment during IVM showing beneficial effects on oocyte and embryonic development. Pioglitazone will contribute to enhancing efficiency of in vitro production.
Supplement of Allicin during IVM Enhances the Oocyte Maturation and Developmental Competence
Sang-Gi Jeong,Seung-Eun Lee,Yun-Gwi Park,Won-Jae Kim,Jae-Wook Yoon,Chan-Oh Park,Hyo-Jin Park,Dong-Yong Hong,Eun-Young Kim,Se-Pill Park 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
The efficiency of the in vitro production of oocytes is significantly lower than that of in vivo culture. Allicin (AL) is generally known to regulate the cellular redox, proliferation, viability, and cell cycle of different cells against extracellular-derived stress. This study performed to reveal the effects of allicin treatment on porcine oocyte maturation and developmental competence. Cumulus oocyte complexes were cultured in IVM medium, containing 0, 0.01, 0.1, 1, 10, and 100 μM AL for 44 h. The rate of polar body emission was tended to be higher in the 0.1 AL-treated group than in the control group (p<0.1). The rates of cleavage and blastocyst formation significantly increased in the 0.1 AL-treated group (p<0.05). The reactive oxygen species level at metaphase II did not significantly differ among all groups. The expression of maternal marker (BMP15 and CCNB1) and anti-apoptotic genes significantly increased in the 0.1 AL-treated group at MII stage, respectively (p<0.05). The rate of phosphorylated p44/42 MAPK to p44/42 MAPK was ~4-fold higher in the 0.1 AL-treated group than in the control group (p<0.05). Consequently, these results indicate that supplementation of oocyte maturation medium with allicin during IVM improves the maturation of oocytes and the subsequent developmental competence of porcine oocytes. In conclusion, allicin enhances the efficiency of IVM to establish a novel IVC system, and we suggested that it will be helpful reagent as assisted reproductive technology (ART) in the future.