http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
정수영,유현희,김금숙,신미경 원광대학교 생활자원개발연구소 2005 생활자원개발연구 Vol.7 No.-
The purpose of this study is to find out the optimal mixing ratios of amounts of Mal-Cha for preparation of Jeung-Pyun through Sensory, Mechanical characteristics test. The proximate composition of Mal-Cha were a 5.46±0.15% of moisture, 4.43±0.11% of total nitrogen, 7.52±0.21% of crude lipid, 8.74% of crude fiber, 8.51±0.09% of ash. Overall quality in 1.5% Jeung-Pyun showed the worst of 0, 0.5, 1.0% Jeung-Pyuns(p<0.05), especially 1.0% Jeung-Pyun had relatively the best overall quality. But the sweetness, sourness, flavor, hardness, moistness were not significantly different among all Jeung- Pyuns. 0, 0.5, 1.0% Jeung-Pyuns showed the best quality of Jeung-Pyun through sensory, mechanical property. Total color difference was increased as amount of Mal-Cha(p<0.001).
Infectious Keratitis in Patients with Ocular Sjögren’s Syndrome
So Yeon Kim(So Yeon Kim),Seong Hwan Kim(Seong Hwan Kim),Chang Ho Yoon(Chang Ho Yoon),Mee Kum Kim(Mee Kum Kim),Joo Youn Oh(Joo Youn Oh) 대한안과학회 2022 Korean Journal of Ophthalmology Vol.36 No.5
Purpose: To investigate the incidence, characteristics, risk factors, and treatment outcomes for infectious keratitis in patients with ocular Sjögren’s syndrome (SS). Methods: We performed a retrospective chart review of patients who had been followed up for ocular SS in Seoul National University Hospital from 2010 to 2020 and identified cases where infectious keratitis developed. The incidence, demographical and clinical characteristics, risk factors, microbiological profiles, and treatment outcome were investigated, some of which were compared with infectious keratitis cases in the non-SS group. Results: Out of 929 patients with ocular SS, infectious keratitis occurred in 18 eyes (1.94%). All 18 patients were female in the ocular SS group, while 48 out of 100 infectious keratitis patients (48%) were female in the non-SS group (p < 0.01). The mean age at diagnosis of infectious keratitis was 66.1 years in the ocular SS group, which was not different from the non-SS group (57.2 years, p = 0.12). Of risk factors analyzed, the use of therapeutic contact lens was more frequently used in the ocular SS patients, compared to the non-SS patients (67% vs. 11%, p < 0.01). Culture-positivity rate was 50% in the ocular SS group. All culture-proven cases were bacterial infection, one of which was bacterial-fungal coinfection. Infection resolved in all eyes after the mean 29 days of medical treatment, except one that additionally required penetrating keratoplasty with vitrectomy. The visual acuity improved in 15 eyes (83%) after resolution. Infectious keratitis recurred in three patients (17%) during the mean 55.7 months of follow-up. Conclusions: The incidence of infectious keratitis was 1.94% in patients with ocular SS. Most were bacterial infections and resolved by medical treatment. Therapeutic and visual outcomes were favorable, but recurrence occurred in 17%.
Kim, Yu Jeong,Lee, Hyun Ju,Ryu, Jin Suk,Kim, Yun Hee,Jeon, Saewha,Oh, Joo Youn,Choung, Ho Kyung,Khwarg, Sang In,Wee, Won Ryang,Kim, Mee Kum Masson Pub. USA 2018 Cornea Vol.37 No.1
PURPOSE:: To investigate the efficacy and safety of transplantation with biomaterial-free cultured oral mucosal epithelial cell sheets (COMECs) for ocular reconstruction in subjects with total limbal stem cell deficiency. METHODS:: A prospective clinical trial (NCT02149732) was conducted in 8 subjects with total limbal stem cell deficiency after approval from the institutional review board of Seoul National University Hospital (H-0707-043-213) and the Ministry of Food and Drug Safety of Korea. COMECs were prepared in a culture system without the use of any temperature-sensitive polymers or carriers. The COMECs were transplanted without suture fixation. Four subjects underwent penetrating keratoplasty after stabilization of the COMEC transplant. Stable epithelialization, changes in visual acuity, and postoperative complications were evaluated for 6 months. Corneal cytokeratins (K) of 4 subjects who underwent penetrating keratoplasty were stained with an immunofluorescent agent. RESULTS:: The ocular surface was successfully reconstructed in 6 eyes. Complete stable epithelialization was achieved within a mean of 53.6 days. Visual improvement (≥2 lines) was achieved in 62.5% of the eyes. K12 (corneal phenotype), K4, and K13 (mucosal phenotype) were well expressed in grafts after keratoplasty, whereas K1, K8, and K19 were barely expressed. No ocular infections, local tumor formation, or remarkable systemic complications were observed. Ocular reconstruction using COMECs failed in 2 eyes, which had full symblepharon in 4 quadrants. CONCLUSIONS:: Transplanting biomaterial-free COMECs seems to be an efficient and safe procedure to reconstruct the ocular surface in patients who are completely limbal stem cell deficient without a full symblepharon.
Antigenicity of Porcine Cornea in Xenocorneal Transplantation
Kim, Mee Kum,Oh, Joo Youn,Lee, Hyeon Il,Ko, Jung Hwa,Lee, Hyun Ju,Wee, Won Ryang,Lee, Jin Hak Lippincott Williams Wilkins, Inc. 2008 Cornea Vol.27 No.suppl1
PURPOSE:: To investigate antigenicity and immunological rejection in porcine-to-murine xenotransplantation as indicator of biofeasibility of the porcine xenograft for human substitute. MATERIALS AND METHODS:: The α-Gal epitope in corneal tissue and cultured porcine cells was stained with Griffonia simplicifolia I isolectin B4. Porcine corneas was orthotopically transplanted to rats. Graft survival, hematoxylin and eosin, CD4 or CD8 staining, and enzyme-linked immunosorbent assays for interleukins and interferon-&ggr; were evaluated. Cultured porcine keratocytes and endothelial cells were mixed with human sera or peripheral blood mononuclear cells for 72 hours. The amount of complement deposited in cells and the number of 51-chromium-releasing cells were assessed. Gene expression was comparatively analyzed using Platinum pig 13K oligo chip in cultured porcine keratocytes and porcine endothelial cells. RESULTS:: The distribution of α-Gal epitope was localized to the anterior stromal keratocytes. However, α-Gal was highly expressed in keratocytes and endothelial cells after cultivation. Median survival of porcine grafts was 8 days. Neutrophils and monocytes were prominent in stroma. The number of CD8 cells was higher than that of CD4 cells. Interleukin-2 and interferon-&ggr; markedly increased in cornea, lacrimal glands, and cervical lymph nodes after 10 days. Complement deposition and the number of 51-chromium-releasing cells were significantly higher in keratocytes than in endothelial cells. TNFAIP6, CXCL14, and CXCL6 genes were highly expressed in keratocytes compared with in endothelial cells. CONCLUSION:: Porcine-to-rat orthotopic corneal xenograft showed CD8 cell- and innate immune cell-related stromal rejection, suggesting high cytotoxicity of porcine keratocytes.
Kim, Yun-Gon,Oh, Joo Youn,Gil, Geun-Cheol,Kim, Mee Kum,Ko, Jung Hwa,Lee, Sukmook,Lee, Hyun Ju,Wee, Won Ryang,Kim, Byung-Gee IRL Press 2009 Current eye research Vol.34 No.10
<P>PURPOSE: To investigate the expression of alpha-Gal or unidentified non-Gal antigens in pig corneal endothelial cells and keratocytes, we performed the qualitative and quantitative analysis by using mass spectrometry. METHODS: The N-glycans from common adult pig corneal endothelial cells and keratocytes cultured in vitro were directly analyzed by using mass spectrometric approaches. In addition, immunochemical staining was added to confirm the non-Gal antigen expression in pig corneal cells. RESULTS: Totally, 34 of the sialylated N-glycans from pig corneal endothelial cells and 27 from pig keratocytes were identified and observed to contain nonhuman sialic acid, NeuGc as well as NeuAc. In addition, we were able to detect 25 of alpha-galactosylated N-glycan structures (22.2% of total) from the pig corneal endothelial cells and 18 of that (17.5% of total) from the pig keratocytes by using mass spectrometric approaches. On immunofluorescent staining, the expression of sialylated glycans was also observed. CONCLUSIONS: As well as alpha-Gal epitopes, several promising non-Gal antigens were widely expressed on both pig corneal endothelial cells and keratocytes. The detailed structural information of the alpha-Gal and non-Gal epitopes would be a tremendous value to develop a new strategy for the successful corneal xenotransplantation in future.</P>
Mee Kum Kim,Jaelim Lee,Sangwon Hwang,Changwon Kee 대한검안학회 2007 Annals of optometry and contact lens Vol.6 No.1
Purpose: The authors present a newly improved technique for scleral fixation of a foldable posterior chamber intraocular lens. Methods: Intraocular lenses (IOLs) were fixed into sclrera in three patients. The both Prolene needles entered into the external sclera beneath flap 1.5 mm away from the limbus, passing through the pupil, and out through the peripheral clear cornea (ab externo approach) Two loops of sutures were externalized through the clear corneal incision, and anchored to the both haptics. IOL were folded and then inserted. Results: All three patients showed stable position of IOL to final follow-up. Best corrected visual acuities were more than 20/40 in all patients. Conclusions: This approach is easily acquired even by the inexperienced surgeon, reduces operative time and places the lens more precisely in the sulcus. This procedure is considered promising for postoperative early visual rehabilitation.
Kim, Mee Kum,Oh, Joo Youn,Ko, Jung Hwa,Lee, Hyun Ju,Jung, Jin Ho,Wee, Won Ryang,Lee, Jin Hak,Park, Chung-Gyu,Kim, Sang Joon,Ahn, Curie,Kim, Seung-Jun,Hwang, Seung Yong The Korean Academy of Medical Sciences 2009 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.24 No.2
<P>Porcine to rat corneal xenotransplantation resulted in severe inflammation and rejection of the corneal stroma, whereas an allograft showed mainly endothelial cell-associated rejection. We, therefore, investigated and compared the gene expression between porcine keratocytes and corneal endothelial cells. RNA was isolated from primary cultured porcine or human keratocytes and porcine corneal endothelial cells. Gene expression was comparatively analyzed after normalization with microarray method using Platinum pig 13 K oligo chip (GenoCheck Co., Ltd., Ansan, Korea). Real-time polymerase chain reaction (PCR) was performed for <I>C1R</I>, <I>CCL2</I>, <I>CXCL6</I>, and <I>HLA-A</I> in porcine keratocytes and corneal endothelial cells. As a result, upregulated expression more than 2 folds was observed in 1,162 genes of porcine keratocytes versus porcine endothelial cells. Among the immune-regulatory genes, <I>SEMA3C</I>, <I>CCL2</I>, <I>CXCL6</I>, <I>F3</I>, <I>HLA-A</I>, <I>CD97</I>, <I>IFI30</I>, <I>C1R</I>, and <I>G1P3</I> were highly expressed in porcine keratocytes, compared to porcine corneal endothelial cells or human keratocytes. When measured by real-time PCR, the expression of <I>C1R</I>, <I>CCL2</I>, and <I>HLA-A</I> was higher in porcine keratocytes compared to that in porcine corneal endothelial cells. In conclusion, the increased expression of <I>C1R</I>, <I>CCL2</I>, and <I>HLA-A</I> genes in porcine keratocytes might be responsible for the stromal rejection observed in a porcine to rat corneal xenotransplantation.</P>