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Single-Chip 마이크로 콘트롤러를 이용한 이상 전류 검출기
김경렬,나채동,문학룡,유두영 崇實大學校 生産技術硏究所 1993 論文集 Vol.23 No.-
The development of the measurement technology has occupied an important position of not only the electrical, electronic but also scientific technology sphere. In the past, the abnormal current detector was used by the analog method, had many the trouble and the inconvenience of measurement, dissatisfied the accuracy and had difficulty in storing, preserving the data. In this paper, these are improved by using single-chip microcontroller which detects the abnormal current and saves in back-up RAM after digitizing. For the efficient management, processing of the memory, it was used the index method. The data in the memory can be transmitted to the personal computer by the serial communication, and also be independently transmitted by the parallel communication to the personal computer which is interfaced with. The software to control system components are structured in assembly language and C language, in order to process and analyze the software is used in C language.
박창진,양재의,유경열,장용선,김원일 한국환경농학회 2004 한국환경농학회지 Vol.23 No.4
The objective of this research was to develop the adsorbent for heavy metals by activating the bark sample. Barks from pine tree with diameters of 2~4 mm were activated in the muffle furnace under a high relative humidity condition at temperatures of 600~900℃. The removal efficiency of the activated bark (ACTBARK) for Cu and Cd was temperature dependent showing the order of 900℃ > 800℃ > 700℃ > 600℃. The critical temperature was considered to be 900℃ to become an efficient adsorbent for Cu and Cd. The bark samples activated at temperatures lower than 700℃ showed a less removal efficiency than the crude bark The ACTBARK activated at 900℃ removed more Cu and Cd from solution than the commercial activated carbon and charcoal. The ACTBARK (activated at 900℃) adsorbed all of the Cu and Cd in solution with concentrations less than 150 mg/L. The selectivity of the ACTBARK was in the order of Cu>Zn>Ni>Pb>Fe>Cd>Mn.
Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in <i>Arabidopsis</i>
Kim, Sun Hee,Roh, Kyung Hee,Park, Jong-Sug,Kim, Kwang-Soo,Kim, Hyun Uk,Lee, Kyeong-Ryeol,Kang, Han-Chul,Kim, Jong-Bum Hindawi Publishing Corporation 2015 BioMed research international Vol.2015 No.-
<P>Reconstitution of nonnative, very-long-chain polyunsaturated fatty acid (VLC-PUFA) biosynthetic pathways in <I>Arabidopsis thaliana</I> was undertaken. The introduction of three primary biosynthetic activities to cells requires the stable coexpression of multiple proteins within the same cell. Herein, we report that C<SUB>22</SUB> VLC-PUFAs were synthesized from C<SUB>18</SUB> precursors by reactions catalyzed by Δ<SUP>6</SUP>-desaturase, an ELOVL5-like enzyme involved in VLC-PUFA elongation, and Δ<SUP>5</SUP>-desaturase. Coexpression of the corresponding genes (<I>McD6DES, AsELOVL5</I>, and <I>PtD5DES</I>) under the control of the seed-specific vicilin promoter resulted in production of docosapentaenoic acid (22:5 n-3) and docosatetraenoic acid (22:4 n-6) as well as eicosapentaenoic acid (20:5 n-3) and arachidonic acid (20:4 n-6) in <I>Arabidopsis</I> seeds. The contributions of the transgenic enzymes and endogenous fatty acid metabolism were determined. Specifically, the reasonable synthesis of omega-3 stearidonic acid (18:4 n-3) could be a useful tool to obtain a sustainable system for the production of omega-3 fatty acids in seeds of a transgenic T3 line 63-1. The results indicated that coexpression of the three proteins was stable. Therefore, this study suggests that metabolic engineering of oilseed crops to produce VLC-PUFAs is feasible.</P>
Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. isolated from soil
Kim Kyeong Ryeol,Kim Kyung Hyun,Khan Shehzad Abid,Kim Hyung Min,Han Dong Min,Jeon Che Ok 한국미생물학회 2021 The journal of microbiology Vol.59 No.8
Two Gram-stain negative, yellow-pigmented, and mesophilic bacteria, designated strains R7T and R19T, were isolated from sandy and forest soil, South Korea, respectively. Both strains were non-motile rods showing catalase- and oxidase-positive activities. Both strains were shown to grow at 10–37°C and pH 6.0–9.0, and in the presence of 0–1.5% (w/v) NaCl. Strain R7T contained iso-C14:0, iso-C15:0, iso-C16:0, and summed feature 9 (comprising C16:0 10-methyl and/or iso-C17:1 ω9c), whereas strain R19T contained iso-C11:0 3-OH, C16:1 ω7c alcohol, iso-C11:0, iso-C15:0, iso-C16:0, and summed feature 9 (comprising C16:0 10-methyl and/or iso-C17:1 ω9c) as major cellular fatty acids (> 5%). Both strains contained ubiquinone- 8 as the sole isoprenoid quinone and phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid as the major polar lipids. The DNA G + C contents of strains R7T and R19T calculated from their genomes were 66.9 mol% and 68.9 mol%, respectively. Strains R7T and R19T were most closely related to Lysobacter panacisoli C8-1T and Lysobacter niabensis GH34-4T with 98.7% and 97.8% 16S rRNA sequence similarities, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains R7T and R19T formed distinct phylogenetic lineages within the genus Lysobacter. Based on phenotypic, chemotaxonomic, and molecular features, strains R7T and R19T represent novel species of the genus Lysobacter, for which the names Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. are proposed. The type strains of L. arenosi and L. solisilvae are R7T (= KACC 21663T = JCM 34257T) and R19T (= KACC 21767T = JCM 34258T), respectively.
Kim, Sun Hee,Kim, Jong Bum,Jang, Yo Soon,Kim, So Yun,Roh, Kyung Hee,Kim, Hyun Uk,Lee, Kyeong-Ryeol,Park, Jong-Sug Kluwer Academic Publishers 2012 Biotechnology letters. Vol.34 No.2
<P>To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5-like elongases of mammals and fishes. This gene is termed AsELOVL5 and encodes a 294 amino acid protein. When AsELOVL5 was expressed in Saccharomyces cerevisiae, it conferred an ability to elongate γ-linolenic acid (18:3 n-6) to di-homo-γ-linolenic acid (20:3 n-6). In addition, the transformed cells converted arachidonic acid (20:4 n-6) and eicosapentaenpic acid (20:5 n-3) to docosatetraenoic acid (22:4 n-6) and docosapentaenoic acid (22:5 n-3), respectively. These results indicate that the AsELOVL5 gene encodes a long-chain fatty acid elongase capable of elongating C(18)δ6/C(20)δ5 but not C(22) PUFA substrates.</P>
Lee, Kyeong-Ryeol,Kim, Eun-Ha,Jeon, Inhwa,Lee, Yongjik,Chen, Grace Q.,Kim, Hyun Uk Elsevier 2019 INDUSTRIAL CROPS AND PRODUCTS Vol.134 No.-
<P><B>Abstract</B></P> <P>Lesquerella (<I>Physaria fendleri</I>) contains a major unusual hydroxy fatty acid, lesquerolic acid (14-hydroxy-eicos-cis-11-enoic acid, C20:1-OH), at 55–60% of seed oil which has industrial value. The remaining seed oil comprises mainly common fatty acids including α-linolenic acid (octadec-<I>cis</I>-9,12,15-enoic acid, C18:3) at 10.7–15.8%. C18:3 is produced from linoleic acid (octadec-cis-9,12-enoic acid, C18:2) by FATTY ACID DESATURASE3. Previous seed transcriptome analysis uncovers two fatty acid desaturase 3 <I>(FAD3</I>) transcripts, <I>PfFAD3-1</I> and <I>PfFAD3-2</I>. To determine the activity of PfFAD3-1 and PfFAD3-2, <I>PfFAD3-1</I> and <I>PfFAD3-2</I> were introduced into an Arabidopsis FAD3-deficient mutant (<I>fad3-2</I>) which has reduced C18:3 from 20.0% in wild-type to 1.6% in <I>fad3-2</I>. Among 20 T<SUB>2</SUB> transgenic lines expression <I>PfFAD3-1</I>, C18:3 increased variably from 2.5 to 29.9% demonstrating that <I>PfFAD3-1</I> acted as a functional FAD3. Among 32 T<SUB>2</SUB> transgenic lines expressing <I>PfFAD3-2</I>, C18:3 content ranged from 1.0 to 3.6%, showing that PfFAD3-2 failed to recover the loss of C18:3 in <I>fad3-2</I>. Sequence comparison among known FAD3s revealed putative variation in PfFAD3-2 which might cause the absence of PfFAD3-2. In addition, lesquerella accumulates a minor hydroxy fatty acid, densipolic acid (12-hydroxy-octadec-cis-9,15-enoic acid, C18:2OH) at about 1%. C18:2OH has been shown to be produced by a FAD3 in Arabidopsis (AtFAD3) using ricinoleic acid (12-hydroxy-9-cis-octadecenoic acid, 18:1-OH) as substrate. To test if either of PfFAD3s is able to convert C18:1-OH to C18:2-OH, <I>PfFAD3-1</I> or <I>PfFAD3-2</I> was transferred into a CL37 Arabidopsis which already expresses a castor (<I>Ricinus communis</I>) fatty acid hrdroxylase <I>FAH12</I> gene (<I>RcFAH12</I>) and consequently accumulates C18:1-OH and C18:2-OH at 13.7% and 3.4%, respectively. Among 43 transgenic CL37 lines expressing <I>PfFAD3-1</I>, C18:2-OH level varied from 0.2 to 7.2%, and four of these lines exceeded to the background level of 3.4% in CL37. Whereas among 23 transgenic CL37 lines expressing <I>PfFAD3-2</I>, C18:2-OH level ranged from 0.4 to 3.4%, none exceeding 3.4%. The results consist with our notion that <I>PfFAD3-1</I>, not <I>PfFAD3-2</I>, exerts FAD3 function which includes converting C18:1-OH to C18:2-OH. Factors limiting PfFAD3s function in CL37 are discussed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lesquerella PfFAD3-1 gene encodes a functional fatty acid desaturase 3 enzyme. </LI> <LI> PfFAD3-1 desaturates linoleic acid and ricinoleic acid in Arabidopsis. </LI> <LI> Lesquerella PfFAD3-2 protein is intact but does not possess fatty acid desaturase 3 activity. </LI> <LI> PfFAD3-1 may be utilized for future basic and applied research to develop new oilseeds. </LI> </UL> </P>
High-oleic oilseed rapes developed with seed-specific suppression of FAD2 gene expression
Lee, Kyeong-Ryeol,Kim, Eun-Ha,Roh, Kyung Hee,Kim, Jong-Bum,Kang, Han-Chul,Go, Young Sam,Suh, Mi Chung,Kim, Hyun Uk The Korean Society for Applied Biological Chemistr 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.4
Vegetable oil is not only important for its edibility but for industrial purposes. High-oleic vegetable oil is especially useful for making biodiesel because it is highly stable against oxidation. Transgenic oilseed crops with modified fatty acid compositions have been developed with several biotechnological gene-silencing methods. The seed oils with the most successfully altered fatty acid compositions are produced from high-oleic oilseed crops in which FAD2 gene expression is suppressed. Vegetable oil from oilseed rape (Brassica napus) is one of the most commonly used vegetable oils throughout the world and its oleic acid content is moderately high (about 65 %). Therefore, oilseed rape can be modified to produce high-oleic oilseed crops. Oilseed rape has four FAD2 genes, originating from B. rapa and B. oleracea, with nucleotide identities of 88-97 %. To produce transgenic high-oleic oilseed rape, plant transformation vectors were constructed using antisense RNA and RNA interference (RNAi) to modify the BrFAD2-1 gene, and canola-type cultivar Youngsan was transformed with Agrobacterium carrying the vectors. The transgenic lines generated, AS9A, HP15, and HPAS29, showed high-oleic phenotypes, which were stably inherited. Their oleic acid contents increased from 67 (Youngsan) to 78, 85, and 86 %, respectively, and their polyunsaturated fatty acid (PUFA) contents decreased from 24 (Youngsan) to 13, 8, and 6 %, respectively. HPAS29, developed with a combined antisense RNA-RNAi method, produced seed oil with the highest oleic acid and lowest PUFA contents. These transgenic high-oleic oilseed rapes could be useful in the manufacture of high-temperature frying oils and high-quality biodiesel fuel.