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Acute Stress Responsive RGS Proteins in the Mouse Brain
Kim, Gyeong-Wha,Lee, Young-Hyurk,Jeong, Eun-Young,Jung, Soon-Woong,Son, Hyeon-Wi,Lee, Dong-Hoon,Roh, Gu-Seob,Kang, Sang-Soo,Cho, Gyeong-Jae,Choi, Wan-Sung,Kim, Hyun-Joon Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.2
Regulator of G-protein signaling (RGS) proteins play an important role in G-protein coupled receptor (GPCR) signaling and the activity of some GPCRs is modulated via RGS protein levels during stress response. The aim of this study was to investigate changes in RGS protein mRNA expressions in the mouse brain after 2h restraint stress. The mRNA level of 19 RGS proteins was analyzed using real-time PCR in six brain regions, which included the prefrontal cortex, amygdala, hippocampus, hypothalamus, striatum, and pituitary gland, from control and stressed mouse. We found that the level of mRNA of each RGS varied according to brain region and that two to eight RGS proteins exhibited changes in mRNA levels in each brain region by restraint stress. It was also revealed that RGS4 protein amount was consistent with mRNA level, indicating RGS4 protein may have regulatory roles in the acute stress response.
Kim Ok-Hyeon,Lee Gyeong Yun,Kim Kyung Yong,Son Jong In,Jung Tae Woo,정지훈,Bang Joon Seok,김형춘,정윤희,Lee Hyun Jung 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.1
Background Hypoxia is a pathological phenomenon of the central nervous system that occurs during ischemia and stroke, leading to brain cell damage. Astrocytes play an important role in maintaining homeostasis in the brain microenvironment under normal as well as in pathological conditions. 3-hydroxymorphinan (3-HM) is an active metabolite of dextromethorphan (DM) that is widely used in anti-cough remedies and is more potent and safer than DM in the treatment of neurological disorders. However, previous research has not examined whether 3-HM has a protective effect in ischemic condition. Objective In this study, we aimed to confirm the effect of 3-HM on hypoxia-induced astrocyte death. We also investigated the protective mechanism of 3-HM and its potential applicability in ischemia brain injury. Result For the in vitro hypoxic conditions, CoCl2, a hypoxia-mimetic agent, was added to mouse primary astrocytes. 200 μM of CoCl2 showed cytotoxicity, whereas pre-incubation with 3-HM attenuated cell death by CoCl2. Moreover, pretreatment with 3-HM dramatically reduced oxidative stress and mitochondrial damage by CoCl2. Further, 3-HM significantly reduced intracellular calcium influx induced by extracellular glutamate and finally modulated the glutamate released from the astrocytes. Conclusion Taken together, these results suggest that 3-HM has a potential to regulate glutamate release from the astrocytic cell death in hypoxic condition and could be used as a promising preventive or therapeutic agent for ischemic brain injury.
( Kim¸ Hyeon Gyeong ),( Jeong¸ Chang Geun ) 한국경영공학회 2023 한국경영공학회지 Vol.28 No.3
Purpose The purpose of this paper is to produce theoretical implications by presenting a response strategy to reduce turnover intention of Air Force pilots based on preceding research. Methods As such, a survey was conducted on Air Force pilots with at least 24 months of work experience under the Ministry of National Defense, 241 of which were targets of analysis. Results This paper finds that reduction in role ambiguity overload, financial job reward, and role achievement culture had a significant and positive (+) effect on job satisfaction, and power-oriented culture had a significant and negative (-) effect on job satisfaction. Conclusion Air Force pilots' working conditions were identified as a major factor of pilots' job satisfaction. This paper empirically verified operating mechanism of Air Force pilot management, and affirmed its effectiveness and validity of reward systems by verifying that job satisfaction is a partial mediator for working conditions and turnover intention.
Kim, In-Suk,Kim, Hoon-Gu,Kim, Dong Chul,Eom, Hyeon-Seok,Kong, Sun-Young,Shin, Ho-Jin,Hwang, Sang-Hyun,Lee, Eun-Yup,Lee, Gyeong-Won Wiley (Blackwell Publishing) 2008 CANCER SCIENCE Vol.99 No.12
<P>ATP-binding cassette transporter G2 (ABCG2) is the most recently described transporter of the multidrug-resistance pump and it promotes resistance to anticancer drugs such as doxorubicin, mitoxantrone, topotecan, and SN-38. Of the ABCG2 polymorphisms, V12M and Q141K alter the functional activity of the ABCG2 transporter and influence the drug response and various toxicities to chemotherapeutic agents. We therefore evaluated the impact of the ABCG2 V12M and Q141K polymorphisms on the therapeutic outcomes and toxicities of primary rituximab plus cyclophosphamide/doxorubicin/vincristine/prednisone (R-CHOP) therapy in 145 Korean patients with diffuse large B-cell lymphoma (DLBCL). ABCG2 V12M and Q141K genotyping was carried out by pyrosequencing of polymerase chain reaction products. The clinical characteristics, treatment outcomes, toxicities of the patients, and the predictive value of the polymorphisms on response, survival, and adverse events to R-CHOP for 145 patients were analyzed according to the ABCG2 V12M and Q141K polymorphisms. No differences were observed according to ABCG2 Q141K and V12M genotype in patient characteristics, disease characteristics, response, survival, or hematology toxicity profiles in patients with DLBCL who received frontline R-CHOP chemotherapy. On multivariate analysis, grade I-IV diarrhea was statistically significant according to ABCG2 Q141K polymorphism (the QQ genotype vs the QK or KK genotypes; hazard ratio 2.835; 95% confidence interval 1.432-5.613; P = 0.003). This study demonstrates that the ABCG2 Q141K polymorphism may correlate with chemotherapy-induced diarrhea in patients with DLBCL who have received frontline R-CHOP chemotherapy, and this has implications for optimizing treatment with such agents.</P>