Fabrication and characterization of 3D-printed bone-like β-tricalcium phosphate/polycaprolactone scaffolds for dental tissue engineering

Park, &gt,.,Lee, S.J.,Jo, H.H.,Lee, J.H.,Kim, W.D.,Lee, J.Y.,-→Park, S.A. Korean Society of Industrial and Engineering Chemi 2017 Journal of industrial and engineering chemistry Vol.46 No.-

<P>beta-tricalcium phosphate (beta-TCP) and polycaprolactone (PCL) composites were manufactured using the lab-made 3D bioprinting system to produce 50TCP50PCL (50% beta-TCP with 50% PCL) and 70TCP30PCL (70% beta-TCP with 30% PCL) composite scaffolds for bone tissue engineering. The 70TCP30PCL scaffold containing the highest beta-TCP content exhibited rougher morphologies and more porous than the other scaffolds (i.e., PCL and 50TCP50PCL). In vitro studies revealed that cell proliferation and alkaline phosphate activity were improved on the beta-TCP-based composite scaffolds. Our results suggest that our 3-D printed beta-TCP-containing PCL scaffolds would benefit new dental applications or regeneration therapies. (C) 2016 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.</P>

Anti-thrombic activity of Korean herbal medicine, Dae-Jo-Whan and its herbs

Chang GT,Min SY,Kim JH,Kim SH,Kim JK,Kim CH 한의병리학회 2006 대한동의병리학회 학술대회논문집 Vol.2006 No.9

Gonadotropin Releasing Hormone ( GnRH ) 유사체 투여에 의한 상피성 난소종양의 GnRH 막 수용체의 변화

김진우(JW Kim),노덕용(DY Ro),김태응(TE Kim),나덕진(DJ Rah),김사진(SJ Kim),한구택(GT Han),정재근(JK Jung),남궁성은(SE Namkoong),김승조(SJ Kim) 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.4

Gonadropin releasing hormone (GnRH) analog, ([D-Trp^6]-luteinizing hormone-releasing hormone, [D-Trp^6]-LH-RH), can cause regression of hormone-dependent human tumors. These effects are thought to be mediated through inhibition of the gonadotropic and steroid hormone. But, it has been reported recently that this analog has a direct inhibitory effect on the tumor. To evaluate the direct inhibitory effect of GnRH analog on ovarian tumors, the characteristics of membrane receptors for GnRH analog were investigated in tissues of normal human ovaries, human ovarian cancers obtained from surgery, and heteroctransplanted nude mice tumors. The results were as follows; 1. In the heterotransplanted nude mice tumors, the inhibitory effect of GnRH analog on tumor growth was compared with untreated animals and in vivo treated animals with [D-Trp^6]-LH-RH. In the treated group showed significant growth inhibition(P<0.001). 2. Specific binding of [^125I, D-Trp^6]-LH-RH were demonstrated in plasma membranes extracted from 7 human epithelial ovarian carcinomas and heterotransplanted nude mice tumors, but not in normal human ovaries. 3. In the plasma membrane preparations from ovarian cancer, statistical analysis of the binding data showed that the interaction of [D-Trp^6]-LH-RH with the binding sites was consistent with a single class of low affinity (Kd=2.1*10^9M)and high capacity binding sites (Bmax=15.8 M/mg membrane protein). 4. Therapy with [D-Trp^6]-LH-RH significantly decreased the binding capacity of receptors for [D-Trp^6]-LH-RH in heterotransplanted nude mice tumors (P<0.001). 5. We observed possible correlation between GnRH binding site density and the clinical corse of the patients, and found out that GnRH responded group showed a higher GnRH binding sites than that of non responded group(P<0.05). These findings are compatible with the view that [D-Trp^6]-LH-RH might exert some direct inhibitory effects on the growth of human ovarian and heterotransplanted nude mice ovarian tumors. Although the functional role of this specific binding site for [D-Trp^6]-LH-RH in ovarian carcinoma is still obscure, it might be part of an autocrine regulatory system and provide that this malignancy might be responsive to hormonal manipulations.

Transforming Growth Factor- 1에 의한 자궁경부암 세포주들의 세포성장억제 기전

김미란(MR Kim),김진우(JW Kim),한구택(GT Han),이준모(JM Lee),정재근(JK Jung),남궁성은(SE Namkoong),김승조(SJ Kim),이헌영(HY Lee),김수평(SP Kim) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.4

Transforming growth factor- 1 (TGF- 1) is known to be a potent growth inhibitor of many cell types including most epithelial cells. However, the molecular mechanism of TGF- 1 action on cell growth of cervical carcinoma has not yet been elucidated. We assessed the effect of TGF- 1 on the cervical carcinoma cell lines, CUMC-3 and CUMC-6, which we estabilished in our laboratory. The results of this study were as follows: 1) TGF- 1 inhibited the growth dose-dependently at the concentration of 0.1-10 ng/ml in both cell lines. 2) DNA fragmentation and electron microscopic findings showed that TGF- 1 induced apoptosis in both cell lines 3) On northern bolt analysis, c-myc mRNA expression was suppressed by TGF- 1 in both cell lines. The inhibition of c-myc expression by TGF- 1 was more prominent in CUMC-6 than in CUMC-3. 4) Western blot analysis showed that the level of p27Kip1 protein was increased after TGF- 1 treatment in both cell lines. These results suggest that TGF- 1 induce apoptosis in two cervical carcinoma cell lines, CUMC-3 and CUMC-6, by the mechanisms involving down-regulation of c-myc gene and over-expression of p27Kip1 protein. We also suggest that CUMC-3 and CUMC-6 may be a good model for study of the complex mechanism of TGF- 1.

원발성 자궁경부암에서 인유두종 바이러스감염과 p53 종양 억제유전자의 변이와의 관계

김진우(JW Kim),조율희(YH Cho),김봉윤(BY Kim),김재훈(JH Kim),김은중(EJ Kim),한구택(GT Han),남궁성은(SE Namkoong) 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.4

Tumor specimens obtained from 136 patients with primary carcinoma of the uterine cervix were analysed for presence of human papillomavirus(HPV) sequences and for mutation of p53 tumor suppressor gene. HPV detection was done using polymerase chain reaction(PCR) amplication with HPV E6 region type specific primers as well as L1 consensus primers. Mutations of the p53 gene were examined by single-strand conformation polymorphism(SSCP) analysis of PCR products. The results were as follows: 1. PCR showed that 130 of 136(96%) tumors contained oncogenic HPV 16 or 18 sequendce. Three(2%) had an unidentified type of HPV and 3(2%) lacked HPV DNA. Of the 127 tumor specimens cell carcinomas analyzed, only 14(11%) showed HPV 18, in contrast to 122 of 127 (96%) having HPV 16(including 13 tumors coinfected with HPV 16 and 18). Of the 9 cases of adenocarcinomas, HPV 16 and HPV 18 were identified in five cases(56%) each (including 3 tumors coinfected with HPV 16 and 18). These results suggest that HPV 16 is predominant type in cervical squamous cell carcinomas(P

Exogenous DNA Uptake of Boar Spermatozoa by a Magnetic Nanoparticle Vector System

Kim, TS,Lee, SH,Gang, GT,Lee, YS,Kim, SU,Koo, DB,Shin, MY,Park, CK,Lee, DS Blackwell Publishing Ltd 2010 REPRODUCTION IN DOMESTIC ANIMALS Vol.45 No.5

<P>Contents</P><P>The sperm-mediated gene transfer method is applicable to transgenesis in many species that use spermatozoa for reproduction recently, which has been shown various results. In the current study, we show that transgenic porcine embryos can be efficiently produced by employing a simple transfection method that uses magnetic nanoparticles (MNPs). The complexes formed between plasmid DNA and MNPs were bounded on ejaculated boar spermatozoa at a higher efficiency compared to methods using DNA alone or lipofection. Using confocal microscopy, rhodamine fluorophore-labelled MNPs were detected on external surfaces of the spermatozoa membrane, which were bounded on zona pellucida of <I>in vitro</I> maturated oocyte during <I>in vitro</I> fertilization. Electron microscopy revealed that clusters of MNPs were detected in inside of plasma membrane and nucleus of the spermatozoa head. Additionally, we found that magnetofected boar spermatozoa could be fertilized with oocytes <I>in vitro</I> and that the resulting gene of green fluorescent protein was detected in fertilized eggs by genomic PCR analysis. Taken together, these results suggest that MNPs can be used to efficiently introduce a transgene into embryo via spermatozoa.</P>

Single Laser Flow Cytometer를 이용한 세포 표면 항원 ( My 7 ) , 세포내 항원 ( c-myc ) 및 DNA 함량의 동시 측정

한구택(GT Han),유기성(KS You),남궁성은(SE Namkoong),김수평(SP Kim),나종구(JG Na),송승규(SK Song),김승조(SJ Kim),이헌영(HY Lee),(John W.Parker) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.1

Flow cytometry, a useful tool for measuring DNA content and cell differentiation as expressed by cell surface markers, is utilized to measure multiple antigens, especially surface antigen, intracellular oncoprotein, and DNA content, simultaneously. For this simultaneous detection, several methods off ixation and permeabilization have been used with limited values. In this study, 20 ㎍/ml of lysolecithin in 1% paraformaldehyde solution was utilized for fixation and permeabilization of cultured promyelocytic leukemic cells(HL 60). The cells were first stained with phycoerythrin (PE)-conjugated monoclonal antibody to the cell surface My 7 antigen and then were fixed and permeabilized with 20 ㎍/ml of lysolecithin in 1% partormaldehyde solution. After incubation, the fixed and permeabilized cells were stained with monoclonal antibody to intracellular c-myc antigen, which were followed by fluorescein isothiocyanate (FITC)-conjugated secondary antibody. The c-myc stained cells were finally stained for DNA content with 7-amino- actinomycin D(7-AAD). This procedure permits excellent staining for intracellular oncoproteins and preservation of surface antigens with relatively low cofficients of variation (CV) for the G0G1 peak of the DNA histograms and suggests that the sequential staining procedure of surface antigen, intracellular antigen, and DNA content will be extended for the study of correlations with cellular differentiation, expression of oncoproteins, and cell cycle analysis in the cells which are obtained from human malignant diseases using a 488 nm single laser flow cytometry.

분만유도 목적으로 사용한 PGE2질정의 효과

한구택(GT Han),최승도(SD Choi),이진우(JW Lee),나종구(JG Na),김진홍(CH Kim),김수평(SP Kim),이헌영(HY Lee) 대한산부인과학회 1985 Obstetrics & Gynecology Science Vol.28 No.3

The mechanism of the initiation of labor is not yet fully understood, but it is generally believed that some factors such as the changes of the endogenous steroid hormones and the prostaglandin may be responsible for the cervical ripening and myometrial contraction. Nowadays the prostaglandin has been used through various routes to induce labor. In order to clarify the effects of prostaglandin of the labor induction and the mechanism of labor, the PGE2 was administered intravaginally to the 42 pregnant women between 38 and 42 weeks gestation and the levels of estradiol, progesterone and cortisol were measured by RIA from the serum collected before and during labor and after the placental expulsion. The results were as follows; 1. The failure rate of the induced labor by intravaginal PGE2 was 9.5%. 2. The mean durations of labor of the control and the experimental group were 4.27±2.38 hours and 4.10±2.67 hours respectively. 3. There were no significant differences between the Apgar scores of the newborn infants delivered from the control and experimental those from the group. 4. The level of the estradiol of the control and experimental group with successful results measured before labor were 32.1±6.2ng/ml and 38.8±12.1ng/ml respectively, and those measured after placental expulsion were 27.3±4.3ng/ml and 20.6±12.5ng/ml respectively. There was significant decreases in the estradiol levels after labor compared with those before labor. 5. The levels of progesterone of the control and the experimental group with successful results measured before labor were 221.2±58.7ng/ml and 245.8±62.9ng/ml respectively, and those measured after placental expulsion were 170.0±49.6ng/ml and 161.0±76.4ng/ml respectively. There were significant decreases in the progesterone levels after labor compared with those before labor. 6. The levels of cortisol of the control and the experimental group with successful results measured before labor were 33.9±7.0㎍/dl and 40.9±9.8㎍/dl, and those measured after placental expulsion were 63.4±10.2㎍/dl and 69.9±15.4㎍/dl respectively. There were significant increases in the cortisol level before labor compared with those before labor. We conclude that the changes of the endogenous steroid hormones in the women intravaginally administered PGE2 were similar to those in control group and the intravaginal admistration of PGE2 may be the effective method for induction of labor.

Photochemotherapy를 위한 8-Methoxypsoralen과 Ultraviolet A Light의 적정량의 결정

한구택(GT Han),유기성(KS You),최명희(MH Choi),곽은정(EJ Kwak),남궁성은(SE Namkoong),김수평(SP Kim),김승조(SJ Kim),이헌영(HY Lee) 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.7

Photochemotherapy using the photosensitizer and ultraviolet A light is a relatively new and potentially selective experimental approach to the treatment of malignant neoplasms. The combination of 8-methoxypsoralen plus ultraviolet A light (320-400nm) is used in the treatment of a hyperproliferative disease of the skin, due to its antiproliferative and cytotoxic effects associated with the DNA photoaddition of 8-methoxypsoralen. In this study, DNA interstranded cross-links were induced in Hind III-linearized plasmid DNA after in vitro treatment with various concentrations of 8-methoxypsoralen(0.0005-15.0micro g) and ultraviolet A light to determine the usable doses of 8-methoxypsoralen and irradiation doses of ultraviolet A light for its future use in gynecologic neoplasms. The double stranded-DNA formed by 8-methoxypsoralen using 20 KJ/m^2 of ultraviolet A light dose, showing increased proportion of double stranded-DNA with the increasing doses and vice versa. These results suggested that the dosages over 0.125 micro g of 8-methoxypsoralen will be able to use in the treatment for some hynecologic neoplasms under the irradiation of 20 KJ/m^2 of ultraviolet A light.

자궁경부암 환자에서 임상적 병기분류와 MRI 병기분류의 비교

김인배(IB Kim),신재인(JI Shin),고영미(YM Ko),한구택(GT Han),이재문(JM Lee),나종구(JG Na),이헌영(HY Lee),김수평(SP Kim) 대한산부인과학회 1995 Obstetrics & Gynecology Science Vol.38 No.10

The comparison between clinical and MRI staging with the final stage was performed on 74 patients with invasive carcinoma of the uterine cervix who were visited and diagnesel from January 1990 through April 1994 . 41 patients among these patients subsequently received radical abdominal hysterectomy and bilateral pelvic lymph node dissection. The results were as follows; 1. the overall accuracy of clinical stage and MR imaging in staging carcinoma of the cervix , compared with final stage including histopathological results, were 70% (52/74) and 55%(41/74) respectively. 2. By clinical and MRI evaluation the accuracies were 82.9% and 70% respectively for the detection of vaginal extension , 100% and 87.8% respectively for the detection of cervical involvement, and both 100% for the detection of rectal bladder, and urinary tract involvement. 3. In detecting parametrial involvement clinical and MRI finding had an accuracy of 68.3% and 65.9% respectively. 4. The sensitivity and specificity for MRI for the detection of parametrial involvement were 33.3% and 68.4% respectively and its positive and negateive predictive value were 7.7% and 92.9%. 5. The presence or absence of lymph node metastasis can not be detected by clinical eramination alone but, accuracy was 75.6% for the detection of lymph node metastasis by MRI evaluation. We concluded that the clinical staging was superior to MRI staging as a whole when they were compared by final staging and the clinical evaluation for the detection of vaginal extension and cervical involvement was superior to MRI evaluation, but both methods have simillar accuracies for the detection of the parametrical involvements and MRI evaluation was superior to clinical evaluation for the detection of lymph node involvement.