사과산 첨가량에 따른 오리 육수의 품질 특성

김기쁨(Ki-Bbeum Kim),김동석(Dong-Suk Kim),송정식(Jung-Sik Song),최수근(Soo-Keun Choi) 동아시아식생활학회 2011 동아시아식생활학회지 Vol.21 No.2

뽕잎 분말 첨가 쌀 파스타 제조의 최적화에 관한 연구

송은주(Eun Ju Song),김기쁨(Ki Bbeum Kim),이광석(Kwang Suck Lee),최수근(Soo Keum Choi) 한국조리학회 2010 한국조리학회지 Vol.16 No.4

The purpose of this study is to develop fresh pasta added with mulberry leaf powder as functional fresh pasta. Through previous research, the mixture of 40% of flour and 60% of rice powder was optimum for making noodles with mulberry leaf powder. Making fresh pasta with 40% of wheat flour, 60% of rice powder (optimum ratio for making noodles) and mulberry leaf powder(0.5%, 1.0%, 1.5%, 2.0%) was done, followed by the mechanical test(moisture content, color value, texture, tension) and the sensory analysis(quantitative descriptive analysis, preference test). Moisture contents of raw pasta and cooked pasta were the highest in control; scores for moisture contents of cooked pasta were higher than those of raw pasta. The result indicated that the more mulberry leaf powder was, the lower L-value and a-value were in raw pasta and cooked pasta. While the b-vale(yellowness) of raw pasta was the highest in control(9.81), 1.0% of mulberry powder addition sample was the highest in cooked pasta. For hardness, the 2.0% of mulberry leaf powder addition sample has high scores, and adhesiveness and chewiness were no significant difference. The 0.5% of mulberry leaf powder addition sample was the longest in tension distance, which was resulted from the lack of water contents in mulberry leaf powder. In cooked pasta, tension distance had no significant difference between the samples, and force showed the highest score in control. The quantitative descriptive analysis showed that color intensity, savory taste, bitterness were the highest in the 2.0% of mulberry leaf powder addition sample. Gloss and chewiness were no significant difference between the samples. Grassy flavor, savory flavor, bitterness and grainess were intense as mulberry leaf powder was added. The preference test showed that MRP 1.5 containing 1.5% of mulberry leaf powder was the most preferable for color, texture and overall quality. In conclusion, 40% of wheat flour, 60% of rice powder and 1.5% of mulberry leaf powder made the best formula of fresh pasta with mulberry leaves.

Anti-apoptotic Activity of Ginsenoside Rb1 in Hydrogen Peroxide-treated Chondrocytes: Stabilization of Mitochondria and the Inhibition of Caspase-3

Ji-Young Na,김석호,Ki-Bbeum Song,Kyu Hee Lim,Gee-Wook Shin,김종훈,Bum Seok Kim,Young-Bae Kwon,Jung-Kee Kwon 고려인삼학회 2012 Journal of Ginseng Research Vol.36 No.3

Chondrocyte apoptosis has been recognized as an important factor in the pathogenesis of osteoarthritis (OA). Hydrogen peroxide (H2O2), which produces reactive oxygen species, reportedly induces apoptosis in chondrocytes. The ginsenoside Rb1 (GRb1)is the principal component in ginseng and has been shown to have a variety of biological activities, such as anti-arthritis, antiinfl ammation, and anti-tumor activities. In this study, we evaluated the effects of G-Rb1 on the mitochondrial permeability transition (MPT) and caspase-3 activity of chondrocyte apoptosis induced by H2O2. Cultured rat articular chondrocytes were exposed to H2O2with or without G-Rb1 and assessed for viability, MPT, Bcl-xL/Bax expression, caspase-3 activity, and apoptosis. The co-treatment with G-Rb1 showed an inhibition of MPT, caspase-3 activity, and cell death. Additionally, the levels of the apoptotic protein Bax were signifi cantly lower and the levels of the anti-apoptotic protein Bcl-xL were higher compared with H2O2 treatment alone. The results of this study demonstrate that G-Rb1 protects chondrocytes against H2O2-induced apoptosis, at least in part via the inhibition of MPT and caspase-3 activity. These results demonstrate that G-Rb1 is a potentially useful drug for the treatment of OA patients.

COMP-Ang1 accelerates type 2 collagen for maintain normal phenotype in hydrogen peroxide induced rat articular chondrocytes

Sokho Kim(김석호),Dea-Seung Choi,Ki-Bbeum Song,Jong-Hoon Kim,Jeong-Chae Lee,Jungkee Kwon 한국실험동물학회 2011 한국실험동물학회 학술발표대회 논문집 Vol.2011 No.2

Anti-apoptotic Activity of Ginsenoside Rb₁ in Hydrogen Peroxide-treated Chondrocytes: Stabilization of Mitochondria and the Inhibition of Caspase-3

Na, Ji-Young,Kim, Sok-Ho,Song, Ki-Bbeum,Lim, Kyu-Hee,Shin, Gee-Wook,Kim, Jong-Hoon,Kim, Bum-Seok,Kwon, Young-Bae,Kwon, Jung-Kee The Korean Society of Ginseng 2012 Journal of Ginseng Research Vol.36 No.3

Chondrocyte apoptosis has been recognized as an important factor in the pathogenesis of osteoarthritis (OA). Hydrogen peroxide ($H_2O_2$), which produces reactive oxygen species, reportedly induces apoptosis in chondrocytes. The ginsenoside $Rb_1$ (G-$Rb_1$) is the principal component in ginseng and has been shown to have a variety of biological activities, such as anti-arthritis, anti-inflammation, and anti-tumor activities. In this study, we evaluated the effects of G-$Rb_1$ on the mitochondrial permeability transition (MPT) and caspase-3 activity of chondrocyte apoptosis induced by $H_2O_2$. Cultured rat articular chondrocytes were exposed to $H_2O_2$ with or without G-$Rb_1$ and assessed for viability, MPT, Bcl-xL/Bax expression, caspase-3 activity, and apoptosis. The co-treatment with G-$Rb_1$ showed an inhibition of MPT, caspase-3 activity, and cell death. Additionally, the levels of the apoptotic protein Bax were significantly lower and the levels of the anti-apoptotic protein Bcl-xL were higher compared with $H_2O_2$ treatment alone. The results of this study demonstrate that G-$Rb_1$ protects chondrocytes against $H_2O_2$-induced apoptosis, at least in part via the inhibition of MPT and caspase-3 activity. These results demonstrate that G-$Rb_1$ is a potentially useful drug for the treatment of OA patients.

퇴행성 골관절염에 대한 HPL-04의 효과

나지영(Ji-Young Na),송기쁨(Ki-Bbeum Song),김석호(Sukho Kim),권영배(Young-Bae Kwon),김대기(Dae-Gi Kim),이준경(Jun-Kyoung Lee),조형권(Hyoung-Kwon Jo),권중기(Jungkee Kwon) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.1

본 연구에서는 HPL-04가 골관절염의 예방 및 치료 약물로서의 가능성을 탐색하여 다음과 같은 결론을 얻었다. HPL-04는 연골세포 생존율과 연골형성과 관련된 collagen type Ⅱ, SOX 9 그리고 aggrecan의 유전자 발현을 유의성 있게 증가시킬 뿐만 아니라 염증성 인자와 관련 있는 MMP-2, 9도 유의성 있게 감소시켰다. HPL-04는 MIA에 의해 유도된 퇴행성 골관절염에서 관절연골의 파괴와 골 침식 등 연골의 변성을 억제했으며 proteoglycan의 소실을 유의성 있게 감소시켰다. 이에 본 연구는 HPL-04가 부작용이 적고 약리 효과가 뛰어나 골관절염 예방 및 치료제 개발에 활용될 수 있을 것으로 본다. HanPoong Leading (HPL)-04 were prepared with different oriental medicines (balk of Kalopanax pictus balk, Chaenomelis Fructus, Angelica gigas root, Zingiber officinale, Raphanus sativus Linne and Saururus chinensis Baill.) to investigate the protective effects of HPL-04 on cartilage degradation in knee osteoarthritis (OA). Rat articular chondrocytes incubated with rhIL-1α markedly increased matrix metalloproteinase (MMP)-2 and 9 activities, decreased cell viability and reduced chondrogenic gene expression. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, MMP-2 and 9 activities and real time RT-PCR indicated that HPL-04 counteracted these harmful effects in dose-dependent manner. In addition, for experimental OA in vivo, monosodium iodoacetate (MIA, 0.5 mg/50 μL) was injected into knee joints of rats and administered HPL-04 to rats for 4 consecutive weeks after MIA treatment. The experimental data showed that treatment with HPL-04 significantly prevented of MMP-2 and 9 activities in articular cartilage. Histopathological and micro-CT evaluations of the knee joints also revealed that HPL-04 effectively ameliorated MIA-induced degenerative OA. In conclusion, HPL-04 has potential applicability for the prevention and treatment of degenerative OA.

Protective Effect of Ginsenoside Rb₁ on Hydrogen Peroxide-induced Oxidative Stress in Rat Articular Chondrocytes

Sokho Kim,Ji-Young Na,Ki-Bbeum Song,Dea-Seung Choi,Jong-Hoon Kim,Young-Bae Kwon,Jungkee Kwon 고려인삼학회 2012 Journal of Ginseng Research Vol.36 No.2

The abnormal maturation and ossifi cation of articular chondrocytes play a central role in the pathogenesis of osteoarthritis (OA). Inhibiting the enzymatic degradation of the extracellular matrix and maintaining the cellular phenotype are two of the major goals of interest in managing OA. Ginseng is frequently taken orally, as a crude substance, as a traditional medicine in Asian countries. Ginsenoside Rb₁, a major component of ginseng that contains an aglycone with a dammarane skeleton, has been reported to exhibit various biological activities, including anti-infl ammatory and anti-tumor effects. However, a chondroprotective effect of ginsenoside Rb1 related to OA has not yet been reported. The purpose of this study was to demonstrate the chondroprotective effect of ginsenoside Rb1 on the regulation of pro-infl ammatory factors and chondrogenic genes. Cultured rat articular chondrocytes were treated with 100 μM ginsenoside Rb1 and/or 500 μM hydrogen peroxide (H2O2) and assessed for viability, reactive oxygen species production, nitric oxide (NO) release, and chondrogenic gene expression. Ginsenoside Rb1 treatment resulted in reductions in the levels of pro-infl ammatory cytokine and NO in H₂O₂-treated chondrocytes. The expression levels of chondrogenic genes, such as type Ⅱ collagen and SOX9, were increased in the presence of ginsenoside Rb1, whereas the expression levels of infl ammatory genes related to chondrocytes, such as MMP1 and MMP13, were reduced by approximately 50%. These results suggest that ginsenoside Rb₁ has potential for use as a therapeutic agent in OA patients.

Protective Effect of Ginsenoside Rb₁ on Hydrogen Peroxide-induced Oxidative Stress in Rat Articular Chondrocytes

Kim, Sok-Ho,Na, Ji-Young,Song, Ki-Bbeum,Choi, Dea-Seung,Kim, Jong-Hoon,Kwon, Young-Bae,Kwon, Jung-Kee The Korean Society of Ginseng 2012 Journal of Ginseng Research Vol.36 No.2

The abnormal maturation and ossification of articular chondrocytes play a central role in the pathogenesis of osteoarthritis (OA). Inhibiting the enzymatic degradation of the extracellular matrix and maintaining the cellular phenotype are two of the major goals of interest in managing OA. Ginseng is frequently taken orally, as a crude substance, as a traditional medicine in Asian countries. Ginsenoside $Rb_1$, a major component of ginseng that contains an aglycone with a dammarane skeleton, has been reported to exhibit various biological activities, including anti-inflammatory and anti-tumor effects. However, a chondroprotective effect of ginsenoside $Rb_1$ related to OA has not yet been reported. The purpose of this study was to demonstrate the chondroprotective effect of ginsenoside $Rb_1$ on the regulation of pro-inflammatory factors and chondrogenic genes. Cultured rat articular chondrocytes were treated with 100 ${\mu}M$ ginsenoside $Rb_1$ and/or 500 ${\mu}M$ hydrogen peroxide ($H_2O_2$) and assessed for viability, reactive oxygen species production, nitric oxide (NO) release, and chondrogenic gene expression. Ginsenoside $Rb_1$ treatment resulted in reductions in the levels of pro-inflammatory cytokine and NO in $H_2O_2$-treated chondrocytes. The expression levels of chondrogenic genes, such as type II collagen and SOX9, were increased in the presence of ginsenoside $Rb_1$, whereas the expression levels of inflammatory genes related to chondrocytes, such as MMP1 and MMP13, were reduced by approximately 50%. These results suggest that ginsenoside $Rb_1$ has potential for use as a therapeutic agent in OA patients.