Studies on the Effects of Biomedicinal Agents on Serum Concentration of Ca2+ , P and ALP Activity in Osteoporosis-Induced Rats

Sang-keun Kim,Myung-hun Lee,Man-hee Rhee 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

on the Effects of Biomedicinal Agents on Serum Concentration of Ca2+, P andALP Activity in Osteoporosis-Induced RatsSang-keun Kim, Myung-hun Lee1 and Man-hee Rhee2College of Veterinary Medicine, Chungnam National University, 220 Gungdong, Yusong-Gu, Daejeon 305-764, Korea1National Veterinary Research and Quarantine Service, Anyang 430-824, Korea2College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, KoreaReceived April 3, 2003 / Accepted July 9, 2003J. Vet. Sci. (2003), 4(2), 151-154JOURNAL OFVeterinaryScience*Corresponding author: Sang-keun Kim Dept. of Vet. Med., Chungnam National University, Daejeon 305764, Korea Tel: +82-42-821-6754, Fax: +82-42-821-6754 E.-mail: kskkim@cnu.ac.kr

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

Physical and chemical Effects on the sonication Treatment of chitosan solution

Lee, Keun Tai,Park, Seong Min,Park, Chan Kyu,Kim, Sang Moo 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.2

물리적 방법을 이용한 키토산 올리고당의 제조에 관한 기초 자료를 제시하기 위하여 키토산용액을 20kHz의 초음파로 처리하였으며, 초음파 처리 효과에 미치는 처리 조건의 영향을 조사하였다. 키토산 용액의 고유점도는 초음파 처리 5분까지는 급속하게 감소하였고 그 이후로는 서서히 감소하였다. 키토산 용액의 부피가 작을수록 초음파 처리효과는 컸으며, 키토산 용액의 온도는 초음파 처리에 별다른 효과가 없는 것으로 나타났다. 5분간 초음파 처리 후의 점도는 용매의 종류에 따라 큰 차이가 없었으나, acetate buffer의 경우 초음파 처리효과가 가장 컸다. pH가 높을수록 초음파 처리효과는 크게 나타났으며, 이온강도와 염의 종류에 따른 효과는 나타나지 않았다. 이상의 결과에서, 용액의 부피는 10~20ml, 온도는 20~30℃, 용액의 pH는 4.5, 그리고 용매의 종류는 acetate buffer로 하는 것이 최적 초음파 처리조건으로 추정된다. As the first step of studies related to production of chitooligosaccharides by physical methods, chitosan solution were sonicated with 20kHz and various treatment effects were examined to present fundamental data of sonicated chitosan solution. Intrinsic viscosity of chitosan solution sharply decreased from 3.76dl/g to 2.90dl/g until 5 minutes of sonication and then slowly decreased. With low volume of chitosan solution, sonication was very effective and temperature of chitosan solution slightly affected the efficiency of sonication. In case of changing the solvent, no significant differences were observed on the effect of sonication, however, acetate buffer had highest sonication effect among various solvents. The sonication effect was increased as the increasement of the value of pH, on the contrary, ionic strength and type of counterions showed no effect on sonication, With these results, we assumed that optimal sonication treatment would be as follows, solution volume was 10 ~20ml, temperature range was 20~30℃ , pH value of solution was 4.5 and type of solvent was acetate buffer

Effect of Nicotinic Acid on Fresh Semen Characteristics in Miniature Pigs

Yeon-Ju Lee,Sang-Hee Lee,Eunsong Lee,Seung Tae Lee,Hee-Tae Cheong,Boo-Keun Yang,Seunghyung Lee,Choon-Keun Park 한국수정란이식학회 2014 한국동물생명공학회지 Vol.29 No.4

Objective of this study was to investigate the effect of nicotinic acid (NA) on the characteristics in fresh semen of miniature pig. We evaluated viability, acrosome reaction and mitochondrial integrity of sperm on 0, 3, 7 and 10 days during storage period with nicotinic acid. As results, the survival rate of sperm in 15 mM NA (day 3, 87.8 ± 1.2%; day 5, 84.0 ± 2.7%; day 7, 82.2 ± 0.9%) and 30 mM NA (day 3, 87.7 ± 0.3%; day 5, 84.4 ± 2.5%; day 7, 82.3 ± 0.7%) groups were higher than control and 5 mM NA groups in 3, 7 and 10 days of semen storage. The NA-treated sperm on 10 day was used day for observing acrosome integrity. The survival sperm with acrosome reaction was higher in 30 mM NA group (day 3, 2.7 ± 0.2%; day 5, 3.3 ± 0.6%; day 7, 11.4 ± 0.3%) than in the control, significantly (P<0.05). Moreover, the live sperm with mitochondrial integrity was higher in whole treatment groups of NA than control group, significantly (P<0.05). Specially, most mitochondrial integrity on 10 day of semen storage was significantly higher in 30 mM NA group (90.2 ± 1.6%) than other treatment groups (control, 81.8 ± 3.1%; 5 mM NA, 83.4 ± 3.0%; 15 mM NA, 89.1 ± 0.7%, P<0.05). In conclusion, supplement of NA in liquid semen of miniature pig can improve and maintain semen quality, such as viability, acrosome reaction, and mitochondria integrity.

Hydroxycinnamoylmalic Acids and Their Methyl Esters from Pear (<i>Pyrus pyrifolia</i> Nakai) Fruit Peel

Lee, Ki Hoon,Cho, Jeong-Yong,Lee, Hyoung Jae,Ma, Young-Kyu,Kwon, Joseph,Park, Seong Hwa,Lee, Sang-Hyun,Cho, Jeong An,Kim, Wol-Soo,Park, Keun-Hyung,Moon, Jae-Hak American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Two novel caffeoylmalic acid methyl esters, 2-<I>O</I>-(<I>trans</I>-caffeoyl)malic acid 1-methyl ester (<B>6</B>) and 2-<I>O</I>-(<I>trans</I>-caffeoyl)malic acid 4-methyl ester (<B>7</B>), were isolated from pear (<I>Pyrus pyrifolia</I> Nakai cv. Chuhwangbae) fruit peels. In addition, 5 known hydroxycinnamoylmalic acids and their methyl esters were identified: 2-<I>O</I>-(<I>trans</I>-coumaroyl)malic acid (<B>1</B>), 2-<I>O</I>-(<I>cis</I>-coumaroyl)malic acid (<B>2</B>), 2-<I>O</I>-(<I>cis</I>-coumaroyl)malic acid 1-methyl ester (<B>3</B>), 2-<I>O</I>-(<I>trans</I>-coumaroyl)malic acid 1-methyl ester (<B>4</B>), and 2-<I>O</I>-(<I>trans</I>-caffeoyl)malic acid (phaselic acid, <B>5</B>). The chemical structures of these compounds were determined by spectroscopic data from ESI MS and NMR. Of all the isolated compounds, five hydroxycinnamoylmalic acids and their methyl esters (<B>2</B>–<B>4</B>, <B>6</B>, <B>7</B>) were identified in the pear for the first time.</P>

The role of Gadd45β in sepsis : modulation of macrophage via mTOR signaling pathway

Sung Je Moon,Hyeon Jong Kim,Ji Hyeon Choi,Jung Hyeon Choi,In Bok Lee,Young Keun Choi,Yong Hoon Kim,Jung Hwan Hwang,Chul Ho Lee 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.08

Development of a Screening System for Plant Defense-Inducing Agent using Transgenic Tobacco Plant with PR-la Promoter and GUS Gene

Sang-Keun Oh,Seon-Woo Lee,Sukyoon Kwon,Doil Choi 한국식물병리학회 2005 Plant Pathology Journal Vol.21 No.3

Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus)

Sang Tae Shin,Sung Keun Jang,Hong Suk Yang,Ok Keun Lee,Yhong Hee Shim,Won Il Choi,Doo Soo Lee,Gwan Sun Lee,Jong Ki Cho,Young Won Lee 대한수의학회 2008 Journal of Veterinary Science Vol.9 No.1

This study was performed to produce transgenic Korean native goat (Capra hircus) by laparoscopic embryo transfer (ET) to overcome the limitations of ET performed by laparotomy. Transgenic embryos were produced by DNA pronuclear microinjection of in vivo zygotes. The recipient goats were synchronized for estrus by using an introvaginal progesterone devices as a controlled internal drugreleasing insert (CIDR) for 13 days and injection of 400 IU PMSG 48 h before removal of the insert. Embryos were transferred on day 3 and 4 after removal of the insert. Recipient goats were deprived of feed for 48 h, then suspended in a laparotomy cradle at an angle of 45o. After obtaining a sufficient pneumoperitoneum, the laparoscope and forceps were inserted abdominally through 5 mm trocar sleeves. Examination of the ovaries and uterus was performed and then 213 embryos were transferred into the oviducts via the infundibula of 76 recipient goats. To compare pregnancy rates, ET was also performed by laparotomy in 82 recipient goats. The pregnancies in the recipient goats were diagnosed by ultrasound on day 30 after embryo transfer. The pregnancy rate with laparoscopic ET was significantly higher than with ET performed by laparotomy (46.1% vs. 28.6%, p < 0.05). In addition, the pregnancy rates were compared between ovulated and non-ovulated ovaries of the recipient goats in the laparoscopic ET group. No significant difference was observed between the pregnancy rates of ovulated and non-ovulated ovaries (41.3% vs. 33.3%, p < 0.05) suggesting that ET may also be possible in non-ovulated recipients through artificial rupture of Graafian follicles. These results suggest that laparoscopic ET is a highly efficient method for the transfer of goat embryos. This study was performed to produce transgenic Korean native goat (Capra hircus) by laparoscopic embryo transfer (ET) to overcome the limitations of ET performed by laparotomy. Transgenic embryos were produced by DNA pronuclear microinjection of in vivo zygotes. The recipient goats were synchronized for estrus by using an introvaginal progesterone devices as a controlled internal drugreleasing insert (CIDR) for 13 days and injection of 400 IU PMSG 48 h before removal of the insert. Embryos were transferred on day 3 and 4 after removal of the insert. Recipient goats were deprived of feed for 48 h, then suspended in a laparotomy cradle at an angle of 45o. After obtaining a sufficient pneumoperitoneum, the laparoscope and forceps were inserted abdominally through 5 mm trocar sleeves. Examination of the ovaries and uterus was performed and then 213 embryos were transferred into the oviducts via the infundibula of 76 recipient goats. To compare pregnancy rates, ET was also performed by laparotomy in 82 recipient goats. The pregnancies in the recipient goats were diagnosed by ultrasound on day 30 after embryo transfer. The pregnancy rate with laparoscopic ET was significantly higher than with ET performed by laparotomy (46.1% vs. 28.6%, p < 0.05). In addition, the pregnancy rates were compared between ovulated and non-ovulated ovaries of the recipient goats in the laparoscopic ET group. No significant difference was observed between the pregnancy rates of ovulated and non-ovulated ovaries (41.3% vs. 33.3%, p < 0.05) suggesting that ET may also be possible in non-ovulated recipients through artificial rupture of Graafian follicles. These results suggest that laparoscopic ET is a highly efficient method for the transfer of goat embryos.

Numerical study on flow characteristic of the KSTAR PF cryogenic loop using supermagnet code

Lee, Hyun Jung,Oh, Sang Jun,Jung, Laurent,Oh, Dong-keun Elsevier 2017 Fusion engineering and design Vol.125 No.-

<P><B>Abstract</B></P> <P>Operation stability study for a large-scale superconducting magnet is of particular importance to protect the magnet from permanent damage. Especially, for a fusion magnet, severe heat load by AC loss needs to be effectively removed during operation to achieve required temperature margin. A unique feature of KSTAR PF (poloidal field) magnet is that all the supercritical helium inlets and outlets of PF1∼4UL (PF upper and lower) coils are located at high field region. Therefore, major heat loads are localized both at the inlets and outlets, so that a drastically reduced or even no temporal cryogen flow is frequently observed at the inlets of PF1∼4UL coils. This kind of temporal blockage of cryogenic loop can seriously affect the thermo-hydraulic analysis of a superconducting magnet system. As a preliminary study on this issue, we compared thermos-hydraulic analysis results of a simplified PF1UL model with a semi full scale PF1∼4UL model by using the SUPERMAGNET code. The numerical simulations were also compared to empirical results.</P>

Common genetic polymorphisms of microRNA biogenesis pathway genes and risk of breast cancer: a case–control study in Korea

Sung, Hyuna,Lee, Kyoung-Mu,Choi, Ji-Yeob,Han, Sohee,Lee, Ji-Young,Li, Lian,Park, Sue K.,Yoo, Keun-Young,Noh, Dong-Young,Ahn, Sei-Hyun,Kang, Daehee Springer-Verlag 2011 Breast cancer research and treatment Vol.130 No.3