Therapeutic Effect of Autologous Activated Platelet-rich Plasma Therapy on Mid-dermal to Full-thickness Burns: A Case Series

Karina Karina,Krista Ekaputri,Johannes Albert Biben,Pritha Hadi,Hubert Andrew,Patricia Marcellina Sadikin 대한성형외과학회 2022 Archives of Plastic Surgery Vol.49 No.3

Although modern medicine hasmade great strides in the management of burn injuries, associated complications such as pain, infection, dyspigmentation, and scarring have yet to be fully dealt with. Although skin grafting and meshing are routinely performed on burn patients, this method poses a risk for adverse effects. Activated autologous platelet-rich plasma (aaPRP), which is increasingly used in the field of plastic surgery, contains growth factors beneficial for wound regeneration. Seven cases of burns with varying severity and conditions that were treated with intralesional subcutaneous injection and intravenous aaPRP are presented and discussed herein. This case series indicates that subcutaneous and intravenous aaPRP is a safe procedure with the potential to be an alternative when skin grafting cannot be done or as an adjunct treatment to skin grafting.

Morphological and anatomical changes in Lilium cv. Arcachon in response to plant growth regulators

Karina Torres-Pio,G umercindo H . D e la Cruz-Guzmán 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3

The eff ect of fi ve concentrations of gibberellic acid (GA 3 ), benzyladenine (BA) and paclobutrazol (PBZ) on the morphologyand anatomy of Lilium cv. Arcachon was evaluated. To this end, bulbs were immersed for 24 h with fi ve diff erent concentrations(0, 25, 50, 100 or 200 mg L −1 ) of GA 3 , BA, or PBZ. Then the treated bulbs were immediately sown in 2.5 L potscontaining tezontle. Pots containing bulbs were randomly distributed, kept under greenhouse conditions, and watered withSteiner solution. Morphological and anatomical traits were evaluated at the fl owering phase. The results showed that height(85.3 cm), number of leaves (79.7), and leaf area (743.3 cm 2 ) were not modifi ed by GA 3 , but they diminished in response toBA. In contrast, stems were 80% smaller in response to PBZ but, did not aff ect the number of fl ower blooms. The biomassof bulbs (27.6 g) raised by 32 and 48% in response to BA and PBZ, respectively. The number of vascular bundles and shootphloem area was similar in all treatments, whereas the number of vessel elements increased by 69% when compared withcontrol plants (62 mm 2 vessels). The length of epidermal cells in the leaves decreased between 30 and 48% in response to bothGA 3 and BA, whereas no eff ects were observed in response to PBZ. Mesophyll width was greater by applying PBZ. Finally,PBZ at 25 mg L −1 is enough to reduce the size of stems and improve their appearance as pot plants. Whereas PBZ aff ectssignifi cantly the size of the stems, the BA provoked bud abortion. This research shows in detail how the growth regulatorsmodifi ed the anatomy of the stems and leaves as well as the roots development in Lilium cv. Arcachon plants.

Improving the Viability of Freeze-dried Probiotics Using a Lysine-based Rehydration Mixture

( Karina Arellano ),( Haryung Park ),( Bobae Kim ),( Subin Yeo ),( Hyunjoo Jo ),( Jin-hak Kim ),( Yosep Ji ),( Wilhelm H. Holzapfel ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.2

The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated “Zeta-bio”, was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

Japanese Perspective on Korean Reunification : An Analysis of Interrelations between Social Identity and Power

Karina Korostelina,Yuji Uesugi 이화여자대학교 국제통상협력연구소 2020 Asian International Studies Review Vol.21 No.1

The paper explores how experts in Japan assess and understand the process and consequences of the unification of the Republic of Korea (South Korea) and the Democratic People’s Republic of Korea (North Korea). Based on the theoretical framework of interrelations between social identity and power, this paper asks how Japanese experts frame the process of Korean unification and evaluate its impact on Japan. The data was collected in Tokyo, Japan, through 37 semi-structured and focus group interviews, then examining these interviews using phenomenological and critical discourse analysis. Analysis of data reveals the existence of four competing narratives rooted in the complex relations between meaning of identity, concepts of power, and Japanese policies toward the unification process. The paper expands the description of two narratives currently present in the existing literature, (1) threat and (2) peace, and introduces two new narratives, (3) democratic processes and (4) restorative justice. The final discussion explores how three groups of factors, (1) regional dynamics, (2) domestic policy, and (3) possible models of unification, influence the prevalence of a particular narrative as well as resulting policies of Japan toward Korean unification.

Development of Chemometric Models Using Infrared Spectroscopy (MID-FTIR) for Detection of Sulfathiazole and Oxytetracycline Residues in Honey

Karina Uribe Hernández,Tzayhrí Gallardo Velázquez,Guillermo Osorio Revilla,Norma Almaraz Abarca,Maylet Hernández Martínez 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4

Chemometric models using mid-infrared (MID-FTIR) spectroscopy were developed for detection and quantification of oxytetracycline and sulfathiazole contamination in honey samples. Low standard error of calibration (SEC) and prediction (SEP) values were achieved using a partial least squares algorithm (SEC= 1.02 and SEP=1.39 for oxytetracycline and SEC=1.24 and SEP=1.79 for sulfathiazole). Chemometric model-predicted concentrations of antibiotics were compared with ELISA results with coefficient of determination R2=0.8577 for oxytetracycline and R2=0.8216 for sulfathiazole. Classification of antibiotic contaminated honey samples and uncontaminated samples was carried out using Soft Independent Modeling Class Analogy analysis with a 100% correct classification rate with interclass distances in the range of 6.93-13.3. MID-FTIR chemometric models developed for detection and quantification of oxitetracycline and sulfathiazole in honey samples have been demonstrated.

Biochemical profile in an infant with neonatal hemochromatosis shows evidence of impairment of mitochondrial long-chain fatty acid oxidation

Karina Lucio de Medeiros Bastos,Caio Robledo Quaio,Fabiana Roberto Lima,Iana Manuelle Araújo,Candice Alves Tavares Araújo,Flávia Balbo Piazzon,Ismael Dale Cotrim Guerreiro da Silva,Gabriel Nuncio Bene 대한간학회 2019 Clinical and Molecular Hepatology(대한간학회지) Vol.25 No.1

Dietary Biotin Supplementation Impairs Testis Morphology and Sperm Quality

Karina Pasten-Hidalgo,Leticia Riveron-Negrete,Gloria Sicilia-Argumedo,Gustavo Canul-Medina,Tonatiuh Salazar-Anzures,Miguel Tapia-Rodrıguez,Enrique O. Hernandez-Gonzalez,Ana Lilia Roa-Espitia,Carlos Ce 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.5

Supplements containing pharmacological concentrations of biotin are commercially available over the counter. Classical toxicity studies have considered biotin administration as harmless; however, recent investigations have shown that biotin supplementation modifies tissue morphology without changes in toxicity markers, raising concerns about the consequences of morphological changes on tissues' functions and the safety of pharmacological concentrations of the vitamin. Testes are very sensitive to toxicants, and testicular histology is a reliable method to study its function. In this work, we investigated the effects of dietary biotin supplementation on testis morphology and spermatogenesis function using an experimental model, in which we have not observed unfavorable effects on other tissue functions or toxicity markers. Male BALB/cAnNHsd mice were fed a control or a biotin-supplemented diet (1.76 or 97.7 mg biotin/kg diet) for 8 weeks. Compared to the control group, the biotin-supplemented mice presented remarkable testis morphology changes, including increased spermatogonia layers; the cellular mechanism involved is related to increased proliferation. Sperm count and serum testosterone levels were not affected, but spermatozoa motility and morphology were significantly impaired in the biotin-supplemented mice. These results caution against the use of supplements with high concentrations of biotin and indicate that biotin's pharmacological effects on morphology need to be considered in toxicological studies.

Synthesis and characterization of sol-gel derived ZrB2-ZrC compounds

Karina S. Campos 한양대학교 세라믹연구소 2014 Journal of Ceramic Processing Research Vol.15 No.6

In this work ZrB2-ZrC compounds were prepared by sol-gel process. These materials were examined by X-ray diffraction (XRD), thermogravimetric (TG) analysis, laser granulometry, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and nitrogen adsorption tests. Thermodynamic calculations were carried out using the FactSage® thermochemical software and databases. XRD revealed that ZrB2 and ZrC are the major phases present in the materials obtained in this study. It was observed that the relative amount of ZrB2 and ZrC in these samples can be tailored by controlling the concentration of H3BO3 in the starting solution. On one hand, laser granulometry and SEM tests revealed that the ZrB2- ZrC compounds prepared in this work show a coarse particle size distribution. On the other hand, they exhibited remarkable specific surface areas (about 150 m2 /g).

Identity and power in Ukraine

Karina V.Korostelina 한양대학교 아태지역연구센터 2013 Journal of Eurasian Studies Vol.4 No.1

This paper analyzes the coercive and legitimate forms of power in Ukraine. It describes the crisis of legitimacy in Ukraine as a contradiction between a blatantly cruel system of capitalism dominated by a few oligarchs, and the lingering remnants of a Soviet mentality. Two strategies are used by the Government to stoke the crisis. First, increased identification with ethnic or regional groups are instrumentally used by the Government to take attention from economic and class issues. Second, the incorporation of a Soviet meaning of power into the new national identity and presentation of it as core norms, believes, and values of the people of Ukraine competes with alternative Ukrainian identity concepts. The paper analyzes five main features of the Soviet meanings of power – political, social, and economic paternalism, perception of power as source of profit and violence, and the dual reality of power with the gap between official narratives of power and a real life. The process of incorporation of the Soviet concept of power into national identity is facilitated by the process of national identity formation that helped to preserve the Soviet perception of power, because of the absence of a new ideology, a lack of critical assessment of the Soviet past, an absence of the vision of outcome, an embryonic culture of democracy, and contributions of all the presidents to the preservation of the Soviet meaning of power. People justify the system as legitimate and fair for many reasons: out of historic habit and deemed moral obligations, self-interests and/or a fear of sanctions, identification with the ruler, zones of indifference, an absence of will and self-confidence, desire to support a strong leader based on ambiguity intolerance, hierarchy – enhancing ideologies, and a general tolerance of injustice. The obedience of subjects is connected with the strength of will of the subjects and the social structures of the society. In Ukraine, the society is not united, not organized, has no identity of “us”, no civic accountability, nor even any real interest in such matters. Civil society levels are very low, as reflected by limited opportunities for civic responsibility and participation and few demands from the society.

Plants cultivated in Choco, Colombia, as source of repellents against Tribolium castaneum (Herbst)

Karina Caballero-Gallardo,Nayive Pino-Benitez,Nerlis Pajaro-Castro,Elena Stashenko,Jesus Olivero-Verbel 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4

Essential oils (EOs) of eight plants collected in Choco, Colombia, including Piper divaricatum, P. pseudolanceifolium,P. confertinodum, P. diazanum, Ocimum campechianum, Siparuna conica, Mikania micrantha and Hedychiumcoronarium, were analyzed by gas chromatography–mass spectrometry and tested as repellents against Triboliumcastaneum, using the area preference method, after 2 and 4 h exposure. The main components found in EOswere methyl eugenol, trans-β-cariophyllene, methyl eugenol, α-pinene, δ-cadinene, γ-elemene, α-pinene and1,8-cineol, for O. campechianum, P. pseudolanceifolium, P. divaricatum, P. confertinodum, P. diazanum, S. conica,M. micrantha, and H. coronarium, respectively. Best repellent activities were observed for oils fromO. campechianum and P. pseudolanceifolium with mean repellent concentration (RC50) values of 0.00006 and0.0001 μL/cm2 after 2 h, and 0.00003 and 0.0001 μL/cm2 after 4 h, respectively; whereas the least potent wasthat from M. micrantha, with RC50 values of 0.074 and 0.040 μL/cm2 at 2 and 4 h exposure times, respectively. Based on average percentage repellence, oils from P. pseudolanceifolium and O. campechianum were classifiedas Class IV repellents and were better than the commercial repellent IR3535, classified as Class II. These data evidencethe Choco region as an important source of natural repellents with promising commercial opportunities.