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Zhang, Jinglan,Shi, Xiaomin,Li, Yehua,Kim, Beom-Jun,Jia, Junling,Huang, Zhiwei,Yang, Tao,Fu, Xiaoyong,Jung, Sung Yun,Wang, Yi,Zhang, Pumin,Kim, Seong-Tae,Pan, Xuewen,Qin, Jun Elsevier 2008 Molecular cell Vol.31 No.1
<P><B>Summary</B></P><P>Sister chromatid cohesion is normally established in S phase in a process that depends on the cohesion establishment factor Eco1, a conserved acetyltransferase. However, due to the lack of known in vivo substrates, how Eco1 regulates cohesion is not understood. Here we report that yeast Eco1 and its human ortholog, ESCO1, both acetylate Smc3, a component of the cohesin complex that physically holds the sister chromatid together, at two conserved lysine residues. Mutating these lysine residues to a nonacetylatable form leads to increased loss of sister chromatid cohesion and genome instability in both yeast and human. In addition, we clarified that the acetyltransferase activity of Eco1 is essential for its function. Our study thus identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion.</P>
Qin, Hua,Gu, Qiang,Kuppu, Sundaram,Sun, Li,Zhu, Xunlu,Mishra, Neelam,Hu, Rongbin,Shen, Guoxin,Zhang, Junling,Zhang, Yizheng,Zhu, Longfu,Zhang, Xianlong,Burow, Mark,Payton, Paxton,Zhang, Hong 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
The Arabidopsis gene AVP1 encodes an $H^+$-pyrophosphatase that functions as a proton pump at the vacuolar membranes, generating a proton gradient across vacuolar membranes, which serves as the driving force for many secondary transporters on vacuolar membranes such as $Na^+/H^+$-antiporters. Overexpression of AVP1 could improve drought tolerance and salt tolerance in transgenic plants, suggesting a possible way in improving drought and salt tolerance in crops. The AVP1 was therefore introduced into peanut by Agrobacterium-mediated transformation. Analysis of AVP1-expressing peanut indicated that AVP1-overexpression in peanut could improve both drought and salt tolerance in greenhouse and growth chamber conditions, as AVP1-overexpressing peanuts produced more biomass and maintained higher photosynthetic rates under both drought and salt conditions. In the field, AVP1-overexpressing peanuts also outperformed wild-type plants by having higher photosynthetic rates and producing higher yields under low irrigation conditions.
Huaqin Sun,Qiang Gu,Sundaram Kuppu,Li Sun,Xunlu Zhu,Neelam Mishra,Rongbin Hu,Guoxin Shen,Junling Zhang,Yizheng Zhang,Longfu Zhu,Xianlong Zhang,Mark Burow,Paxton Payton,Hong Zhang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
The Arabidopsis gene AVP1 encodes an H?-pyrophosphatase that functions as a proton pump at thevacuolar membranes, generating a proton gradient acrossvacuolar membranes, which serves as the driving force formany secondary transporters on vacuolar membranes suchas Na?/H?-antiporters. Overexpression of AVP1 couldimprove drought tolerance and salt tolerance in transgenicplants, suggesting a possible way in improving drought andsalt tolerance in crops. The AVP1 was therefore introducedinto peanut by Agrobacterium-mediated transformation. Analysis of AVP1-expressing peanut indicated that AVP1-overexpression in peanut could improve both droughtand salt tolerance in greenhouse and growth chamberconditions, as AVP1-overexpressing peanuts producedmore biomass and maintained higher photosynthetic ratesunder both drought and salt conditions. In the field, AVP1-overexpressing peanuts also outperformed wild-type plantsby having higher photosynthetic rates and producing higheryields under low irrigation conditions.
Liu Junling,Zhang Yawen 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.10
With the development of network technology and the popularization of mobile terminals, the research and development of M-Learning resources based on iPad-user interaction technology, namely e-textbooks, has attracted increasing attention in global educative reforms. On the basis of the research on iPad’s user-machine interaction technology, this paper takes the bilingual e-textbooks development of The Kazak Alphabet as an example. It proposes basic steps and framework of the research and development and summarizes the key interaction technology and the ways of realization of preparing, developing, packaging and publishing in the hope that it can provide the foundation for the development of M-Learning and bilingual education.
Li, Kuo,Zhang, Junling,Ji, Chunxue,Wang, Lixuan Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.7
MicroRNAs (miRNAs) have been reported to be involved in many neurodegenerative diseases. The present study focused on the role of hsa-miR-144-3p in one of the neuro-degenerative diseases, Parkinson's disease (PD). Our study showed a remarkable down-regulation of miR-144-3p expression in 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-treated SH-SY5Y cells. MiR-144-3p was then overexpressed and silenced in human SH-SY5Y cells by miRNA-mimics and miRNA-inhibitor transfections, respectively. Furthermore, ${\beta}$-amyloid precursor protein (APP) was identified as a target gene of miR-144-3p via a luciferase reporter assay. We found that miR-144-3p overexpression significantly inhibited the protein expression of APP. Since mitochondrial dysfunction has been shown to be one of the major pathological events in PD, we also focused on the role of miR-144-3p and APP in regulating mitochondrial functions. Our study demonstrated that up-regulation of miR-144-3p increased expression of the key genes involved in maintaining mitochondrial function, including peroxisome proliferator-activated receptor ${\gamma}$ coactivator-$1{\alpha}$(PGC-$1{\alpha}$), nuclear respiratory factor 1 (NRF-1) and mitochondrial transcription factor A (TFAM). Moreover, there was also a significant increase in cellular ATP, cell viability and the relative copy number of mtDNA in the presence of miR-144-3p overexpression. In contrast, miR-144-3p silencing showed opposite effects. We also found that APP overexpression significantly decreased ATP level, cell viability, the relative copy number of mtDNA and the expression of these three genes, which reversed the effects of miR-144-3p overexpression. Taken together, these results show that miR-144-3p plays an important role in maintaining mitochondrial function, and its target gene APP is also involved in this process.
A New Approach to Produce Resveratrol by Enzymatic Bioconversion
( Jinxin Che ),( Junling Shi ),( Zhenhong Gao ),( Yan Zhang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.8
An enzymatic reaction system was developed and optimized for bioconversion of resveratrol from glucose. Liquid enzyme extracts were prepared from Alternaria sp. MG1, an endophytic fungus from grape, and used directly or after immobilization with sodium alginate. When the enzyme solution was used, efficient production of resveratrol was found within 120 min in a manner that was pH-, reaction time-, enzyme amount-, substrate type-, and substrate concentration-dependent. After the optimization experiments using the response surface methodology, the highest value of resveratrol production (224.40 ug/l) was found under the conditions of pH 6.84, 0.35 g/l glucose, 0.02 mg/l coenzyme A, and 0.02 mg/l ATP. Immobilized enzyme extracts could keep high production of resveratrol during recycling use for two to five times. The developed system indicated a potential approach to resveratrol biosynthesis independent of plants and fungal cell growth, and provided a possible way to produce resveratrol within 2 h, the shortest period needed for biosynthesis of resveratrol so far.
Lixuan Wang,Kuo Li,Junling Zhang,Chunxue Ji 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.7
MicroRNAs (miRNAs) have been reported to be involved in many neurodegenerative diseases. The present study focused on the role of hsa-miR-144-3p in one of the neurodegenerative diseases, Parkinson’s disease (PD). Our study showed a remarkable down-regulation of miR-144-3p expression in 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-treated SH-SY5Y cells. MiR-144-3p was then overexpressed and silenced in human SH-SY5Y cells by miRNA-mimics and miRNA-inhibitor transfections, respectively. Furthermore, -amyloid precursor protein (APP) was identified as a target gene of miR-144-3p via a luciferase reporter assay. We found that miR-144-3p overexpression significantly inhibited the protein expression of APP. Since mitochondrial dysfunction has been shown to be one of the major pathological events in PD, we also focused on the role of miR-144-3p and APP in regulating mitochondrial functions. Our study demonstrated that up-regulation of miR-144-3p increased expression of the key genes in-volved in maintaining mitochondrial function, including peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1), nuclear respiratory factor 1 (NRF-1) and mitochondrial transcription factor A (TFAM). Moreover, there was also a significant increase in cellular ATP, cell viability and the relative copy number of mtDNA in the presence of miR-144-3p overexpression. In contrast, miR-144-3p silencing showed opposite effects. We also found that APP overexpression significantly decreased ATP level, cell viability, the relative copy number of mtDNA and the expression of these three genes, which reversed the effects of miR-144-3p overexpression. Taken together, these results show that miR-144-3p plays an important role in maintaining mitochondrial function, and its target gene APP is also involved in this process.
Gao Ran,Guo Wenjun,Fan Tianfei,Pang Junling,Hou Yangfeng,Feng Xiaohang,Li Bolun,Ge Weipeng,Fan Tianhui,Zhang Tiantian,Lu Jiakai,Jing He,Jin Mu,Yan Chen,Wang Jing 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Abdominal aortic aneurysm (AAA) is a permanent expansion of the abdominal aorta that has a high mortality but limited treatment options. Phosphodiesterase (PDE) 4 family members are cAMP-specific hydrolyzing enzymes and have four isoforms (PDE4A-PDE4D). Several pan-PDE4 inhibitors are used clinically. However, the regulation and function of PDE4 in AAA remain largely unknown. Herein, we showed that PDE4D expression is upregulated in human and angiotensin II-induced mouse AAA tissues using RT-PCR, western blotting, and immunohistochemical staining. Furthermore, smooth muscle cell (SMC)-specific Pde4d knockout mice showed significantly reduced vascular destabilization and AAA development in an experimental AAA model. The PDE4 inhibitor rolipram also suppressed vascular pathogenesis and AAA formation in mice. In addition, PDE4D deficiency inhibited caspase 3 cleavage and SMC apoptosis in vivo and in vitro, as shown by bulk RNA-seq, western blotting, flow cytometry and TUNEL staining. Mechanistic studies revealed that PDE4D promotes apoptosis by suppressing the activation of cAMP-activated protein kinase A (PKA) instead of the exchange protein directly activated by cAMP (Epac). Additionally, the phosphorylation of BCL2-antagonist of cell death (Bad) was reversed by PDE4D siRNA in vitro, which indicates that PDE4D regulates SMC apoptosis via the cAMP-PKA-pBad axis. Overall, these findings indicate that PDE4D upregulation in SMCs plays a causative role in AAA development and suggest that pharmacological inhibition of PDE4 may represent a potential therapeutic strategy.