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국내 육성 감자의 재분화와 형질전환 효율에 미치는 생장조절제의 조성 및 몇 가지 요소
이정윤,서효원,조지홍,이신우,윤한대,Yi, Jung-Yoon,Seo, Hyo-Won,Cho, Ji-Hong,Lee, Shin-Woo,Yun, Han-Dae 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.1
국내에서 육종된 감자 품종들에 대하여 재분화 및 형질전환효율에 영향을 미치는 여러 요인들을 조사하고 품종별 최적 조건을 규명하고자 하였다. 최근 육종된 5품종 '추백', '남서', '자심', '조풍'과 '조원'의 잎과 줄기 조직을 재료로 사용하여 배지 내 호르몬 조성별 신초형성률을 조사하였다. 신초형성률은 잎 조직의 경우 '조원'이 GA₃0.1 mg/L, Zeatin 2.0mg/L과 NAA 0.01 mg/L가 포함된 M5 배지에서 90%이상으로 가장 높은 반면 다른 공시품종은 20% 미만이었으며 줄기조직을 치상한 경우 '조풍'이 M5 배지에서 70%의 신초형성률을 나타내었으나. 다른 품종은 25% 이하의 신초형성률을 나타내어 품종별, 조직별로 큰 차이를 나타내었다. 감자의 형질전환 시 Agrobacterium 접종과정에서 75 μM의 acetosyringone을 처리한 경우 잎 조직은 '조원'이 87.9%, 줄기 조직은 '조풍'이 68.4%로 높은 형질전환효율을 나타내어 acetosyringone을 처리하지 않은 대조구에 비하여 1.5∼4.0배의 높은 형질전환효율을 나타냈다. The optimal condition of in vitro regeneration and transformation were investigated for newly bred potato varieties in Korea. Leaf and internodal stem tissues of 'Chubak', 'Namsuh', 'Jasim', 'Jopung' and 'Jowon' were used to investigate the influence of growth regulator on regeneration efficiency The effect of phenolic compound acetosyringone on gene transformation efficiency was also investigated. Leaf tissue of 'Jowon' and internodal stem tissues of 'Jopung' were showed high regeneration efficiency on M5 medium supplemented with 0.1 mg/L GA₃, 2.0 mg/L Zeatin and 0.01 mg/L NAA. The other three potato cultivars were showed low regeneration efficiency less than 25%. The effect of acetosyringone on Agrobacterium-mediated gene transformation with leaf and internodal stem tissues were noticeable. By adding the 75 μM of acetosyringone during the Agrobacterium innoculation, the transformation efficiency was increased up to 1.5∼4.0 fold compare to non-treated control. In case of 'Jowon' the transformation efficiency was 87.9% in leaf tissue and 'Jopung' was 68.4% in internodal stem tissues.
이정윤 ( Jung Yoon Yi ),이석영 ( Sok Young Lee ),이호선 ( Ho Sun Lee ),이영이 ( Young Yi Lee ),전영아 ( Young Ah Jeon ),박홍재 ( Hong Jae Park ),김정곤 ( Chung Kon Kim ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.4
Genetic resources can be used through complementary ex situ and in situ conservation approaches organized at the local, national, and global levels. Genetic resources as defined in the Convention on Biological Diversity (CBD) refer to physical, tangible genetic materials as well as genetic information and knowledge. The Plant Genetic Resources Center (PGRC) was established at the Taiwan Agricultural Research Institute in 1993. To 2010, seed collections of 73,275 accessions are being conserved in long- and medium-term storage rooms. In field genebanks, collections and preservation of germplasm of fruit trees and medicinal plants include approximately 5,106 accessions and are being conserved in several repositories. As a complementary conservation, in vitro and cryopreservation have been carried out for the species endangered and hardly preserved in field. The extent of germplasm management is very critical to promote efficient utilization of genetic resources, especially local varieties or breeding lines. Action plans on risk management, safe conservation, species identification, crossing and better utilization of genetic resources for food and agriculture have been formulated to develop markets for various kinds of products. Efficient management and conservation of genetic resources are very important to ensure the present and future security of food and agriculture. As a co-worker in global efforts to conserve genetic resources, we look for greater benefits and responsibilities. Particularly, in point of global climate changes and food crises, actions are needed to equip and manage genetic resource conservation systems as an effective component of national and international food and agriculture security.