中国延边旅游人力资源现状及开发对策

徐哲俊(Xu,Zhe-Jun),金红,英(Xu,Zhe-Jun),金春洙(Kim, Chun-Soo) 글로벌경영학회 2012 글로벌경영학회지 Vol.9 No.3

With the development of Yanbian tourism and entering from all directions of foreign enterprises, which will have a great impact on the development and management of tourism human resources in China. As Yanbian tourism develops rapidly in recent years, the demand for tourism human resources has a rapid expansion. However, the development and management of human resources is relatively backward, which has seriously limited the development of Yanbian tourism and needs to be solved as soon as possible. Therefore, this paper elaborates the human resources of Yanbian, analyses the problems about the development and management of human resources, and then finds that the total quantity of Yanbian human resources is insufficient, whose personnel training has a great gap compared with its market demand;its quality and level as a whole is somewhat low; professionals of tourism and hotel management are deficient;training work falls behind, which can not satisfy the tourism demand;incentive mechanism is on shortage, which leads to a frequent flow of personnel;employees have a weak sense of service. Then, it constructed a “Trinity” pattern of human resources development and management in Yanbian tourism. This paper puts forward practical countermeasure and measures from 6 aspects of establishing the correct concept of tourism talents, letting local governments and tourism administration departments play a leading role sufficiently, improving and strengthening the tourism human resources training, improving Yanbian tourism education system, building perfect talent and incentive mechanism, strengthening the occupation career management of tourism enterprise employee and so on, which provides theoretic and practical reference for the scientific development of human resources in Yanbian. 旅游业的持续快速发展以及国外旅游企业的全方位进入,将对中国旅游人力资源开发与管理 带来极大的冲击。近年来延边旅游业迅猛发展,旅游业对人力资源的需求急剧膨胀,但是,旅 游人力资源的开发与管理的相对滞后,严重制约着延边旅游业的发展,需要迅速加以解决。因 此,本文首先阐述了延边旅游人力资源的现状,分析了旅游人力资源开发与管理中存在的问 题,发现了延边旅游人力资源总量相对不足,人才培养滞后于市场需求;整体素质及层次偏 低;旅游及酒店管理专业人才匮乏;培训工作落后,不能满足旅游业的需求;缺乏有效的激励 机制,人员流动频繁;从业人员服务意识薄弱。然后,构建了延边旅游业“三位一体”人力资源开 发与管理模式。最后,提出了树立正确的旅游人才观念、充分发挥地方政府和旅游行政管理部 门的指导作用、完善和加强旅游人力资源培训、改善延边旅游教育体系、建立完善的人才使用 和激励机制、强化旅游企业员工职业生涯管理等6个方面提出了切实可行的对策和措施,为延边 旅游企业人力资源的科学发展提供理论与实践。鉴。

Seasonal parasitism of Riptortus clavatus Thunberg (Heteroptera: Alydidae) by Dionaea magnifrons (Herting) (Diptera: Tachinidae)

Jun Ki Son,Nguyen Xu Do,박정규 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol.11 No.4

Seasonal parasitism of adult Riptortus clavatus (Thunberg) (Heteroptera: Alydidae) by the tachinid fly parasitoid, Dionaea magnifrons (Herting), was monitored at three sites in Korea, 2006 by using the aggregation pheromone traps of R. clavatus. Basic developmental characteristics of the parasitoid were also examined. Annual parasitism of R. clavatus female and male adults was 6.8 and 11.6%, respectively. Parasitism of females and males of the over-wintering (Oct. to Jun.) generation (10.6 and 16.5%, respectively) was higher than the summer (Jul. to Sep.) generation (2.1 and 2.5%). Maximum parasitism for males was recorded during spring or autumn, reaching 35.6, 25.0, and 26.9% at the university campus, and pear and sweet persimmon orchards, respectively. Sex ratio of all the D. magnifrons was slightly male-dominated (62.2%). The pupal period of D. magnifrons was 9.2 days for both sexes in room temperature conditions. D. magnifrons larvae escaped from R. clavatus in 8.0±3.4 days after host bug collection fromthe field during April to September, whereas in October and November they emerged in 15.1±3.8 days. This parasitoid fly should be studied further to determine its effectiveness as a biological control agent complementary to existing strategies in the management of bean bug. Seasonal parasitism of adult Riptortus clavatus (Thunberg) (Heteroptera: Alydidae) by the tachinid fly parasitoid, Dionaea magnifrons (Herting), was monitored at three sites in Korea, 2006 by using the aggregation pheromone traps of R. clavatus. Basic developmental characteristics of the parasitoid were also examined. Annual parasitism of R. clavatus female and male adults was 6.8 and 11.6%, respectively. Parasitism of females and males of the over-wintering (Oct. to Jun.) generation (10.6 and 16.5%, respectively) was higher than the summer (Jul. to Sep.) generation (2.1 and 2.5%). Maximum parasitism for males was recorded during spring or autumn, reaching 35.6, 25.0, and 26.9% at the university campus, and pear and sweet persimmon orchards, respectively. Sex ratio of all the D. magnifrons was slightly male-dominated (62.2%). The pupal period of D. magnifrons was 9.2 days for both sexes in room temperature conditions. D. magnifrons larvae escaped from R. clavatus in 8.0±3.4 days after host bug collection fromthe field during April to September, whereas in October and November they emerged in 15.1±3.8 days. This parasitoid fly should be studied further to determine its effectiveness as a biological control agent complementary to existing strategies in the management of bean bug.

Biochemical Characterization of a GDSL-Motif Esterase from Bacillus sp. K91 with a New Putative Catalytic Mechanism

( Jun Mei Ding ),( Ting Ting Yu ),( Lian Ming Liang ),( Zhen Rong Xie ),( Yun Juan Yang ),( Jun Pei Zhou ),( Bo Xu ),( Jun Jun Li ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11

The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around 50°C at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an α/β-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.

Molecular and Biochemical Characterization of a Novel Xylanase from Massilia sp. RBM26 Isolated from the Feces of Rhinopithecus bieti

( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1

Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.

Molecular Biology and Omics : Cloning and Characterization of a Novel α-Amylase from a Fecal Microbial Metagenome

( Bo Xu ),( Fu Ya Yang ),( Cai Yun Xiong ),( Jun Jun Li ),( Xiang Hua Tang ),( Jun Pei Zhou ),( Zhen Rong Xie ),( Jun Mei Ding ),( Yun Juan Yang ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4

To isolate novel and useful microbial enzymes from uncultured gastrointestinal microorganisms, a fecal microbial metagenomic library of the pygmy loris was constructed. The library was screened for amylolytic activity, and 8 of 50,000 recombinant clones showed amylolytic activity. Subcloning and sequence analysis of a positive clone led to the identification a novel gene (amyPL) coding for α-amylase. AmyPL was expressed in Escherichia coli BL21 (DE3) and the purified AmyPL was enzymatically characterized. This study is the first to report the molecular and biochemical characterization of a novel α-amylase from a gastrointestinal metagenomic library.

RNAi suppression of nuclear receptor genes results in increased susceptibility to sulfoxaflor in brown planthopper, Nilaparvata lugens

Lu Xu,Chun-Qing Zhao,De-Jin Xu,Guang-Chun Xu,Xiao-Long Xu,Zhao-Jun Han,Ya-Nan Zhang,Zhong-Yan Gu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Nuclear receptors (NRs), which belong to a superfamily of ligand-dependent transcription factors, play essential roles in gene regulation to affect numerous physiological pathways. Twenty NR genes were identified in Nilaparvata lugens by using genomic, transcriptomic and GenBank databases, and categorized into NR0-NR6 subfamilies according to standard nomenclature. Among them, three NR geneswere classed into NR0, four into NR1, eight into NR2, one into NR3, one into NR4, two into NR5, and one into NR6, respectively. A phylogenetic tree of NRs from N. lugens and other representative species was constructed, which provided evolutionary insight into genetic distance. In order to investigate the NRs,whichwere induced by sulfoxaflor, time- and tissue-specific expression profiles of NR genes in fourth-instar nymphs were determined following LD50 sulfoxaflor treatment (0.28 ng/insect) and compared with control samples. NlUSP, NlE78, NlTLL, NlHR51, NlHR83, NlPNR and NlFTZ-F1 were highly expressed following 12–48 h of sulfoxaflor treatment. NlHR39 and NlDSF transcripts were detected in the head and the thorax, respectively. High-level and constitutive expression of NlHR3, NlTLL, NlHR83, NlFTZF1 and NlHR4 were found in the abdomen. Feeding of dsRNAs reduced the expression of NlHR3, NlUSP, NlTLL, NlHR83, NlPNR, NlFTZ-F1 and NlHR4 (35.48–49.77%) and caused significant nymph mortality (69.21–81.45%). These NRs, considered as insecticide targets, may play important function in sulfoxaflor detoxification.

Annealing Effects on the Structural and Electrical Properties of SiOC(-H) Films with Low Dielectric Constant prepared by Plasma Enhanced Chemical Vapor Deposition

Jun Xu,Chi Kyu Choi,Chang Shil Yang 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.45 No.1

Low dielectric constant SiOC(-H) lms have been prepared by plasma enhanced chemical vapor deposition by using bis-trimethylsilyl-methane (BTMSM) and O2 precursors. The annealing eects on the structural and electrical properties were studied. The results indicate post-annealing could eciently remove the hydroxyl (-OH) related groups from the as-deposited lms and cause chemical structure re-arrangement, resulting in more nano-pores being formed in the annealed SiOC(-H) lms. The charge-trapping centers in the interface with Si substrate, as well as in the lm bulk, could be reduced during the annealing procedure, which causes a rightshift of the capacitancevoltage curve. The dielectric constant decreased from 3.1 to 2.1, and the refractive index decreased from 1.427 to 1.321, when the SiOC(-H) lm was annealed at 400 C for 30 minutes. The currentvoltage analysis indicates that the leakage-current density is 3.4 10 A/cm2 at an applied electric eld of 1 MV/cm, and the dominant conduction mechanism is Schottky emission in asdeposited and annealed SiOC(-H) lms at mid electric eld.

Effect of Insulin-like Growth Factor-1 on Bone Morphogenetic Protein-2 Expression in Hepatic Carcinoma SMMC7721 Cells through the p38 MAPK Signaling Pathway

Xu, Guan-Jun,Cai, Sheng,Wu, Jian-Bing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To observe the effect of insulin-like growth factor-1 (IGF-1) on bone morphogenetic protein (BMP)-2 expression in hepatocellular carcinoma SMMC7721 cells. Methods: Cells were divided into blank control, IGF-1, IGF-1 + SB203580, and SB203580 groups. SB203580 was used to block the p38 MAPK signaling pathway. Changes in the expression of BMP-2, p38 MAPK, and phosphorylated p38, MERK, ERK and JNK were determined using reverse transcription polymerase chain reactions (RT-PCR) and Western blot analysis. Results: Protein expression of phosphorylated BMP-2, MERK, ERK, and JNK was significantly up-regulated by IGF-1 compared with the control group ($1.138{\pm}0.065$ vs. $0.606{\pm}0.013$, $0.292{\pm}0.005$ vs. $0.150{\pm}0.081$, $0.378{\pm}0.006$ vs. $0.606{\pm}0.013$, and $0.299{\pm}0.015$ vs. $0.196{\pm}0.017$, respectively; P<0.05). Levels of BMP-2 and phosphorylated MERK and JNK were significantly reduced after blocking of the p38MAPK signaling pathway ($0.494{\pm}0.052$ vs. $0.165{\pm}0.017$, $0.073{\pm}0.07$ vs. $0.150{\pm}0.081$, and $0.018{\pm}0.008$ vs. $0.196{\pm}0.017$, respectively; P<0.05), but such a significant difference was not observed for phosphorylated ERK protein expression ($0.173{\pm}0.07$ vs. $0.150{\pm}0.081$, P>0.05). Conclusion: IGF-1 can up-regulate BMP-2 expression, and p38 MAPK signaling pathway blockage can noticeably reduce the up-regulated expression. We can conclude that the up-regulatory effect of IGF-1 on BMP-2 expression is realized through the p38 MAPK signaling pathway.

Modulator of surface plasmon polariton based cycle branch graphene waveguide

Jun Zhu,Zhengjie Xu,Wenju Xu,Duqu Wei 한국탄소학회 2018 Carbon Letters Vol.25 No.-

At present, an important research area is the search for materials that are compatible with CMOS technology and achieve a satisfactory response rate and modulation efficiency. A strong local field of graphene surface plasmon polariton (SPP) can increase the interaction between light and graphene, reduce device size, and facilitate the integration of materials with CMOS. In this study, we design a new modulator of SPP-based cycle branch graphene waveguide. The structure comprises a primary waveguide of graphene–LiNbO3–graphene, and a secondary cycle branch waveguide is etched on the surface of LiNbO3. Part of the incident light in the primary waveguide enters the secondary waveguide, thus leading to a phase difference with the primary waveguide as reflected at the end of the branch and interaction coupling to enhance output light intensity. Through feature analysis, we discover that the area of the secondary waveguide shows significant localized fields and SPPs. Moreover, the cycle branch graphene waveguide can realize gain compensation, reduce transmission loss, and increase transmission distance. Numerical simulations show that the minimum effective mode field area is about 0.0130l2, the gain coefficient is about 700 cm–1, and the quality factor can reach 150. The structure can realize the mode field limits of deep subwavelength and achieve a good comprehensive performance.

情绪智力对工作压力和工作倦怠的影响

Xu, Zhe-Jun(徐哲俊),Jin, Hong-Ying(金红,英),Kim, Chun-Soo(金春洙) 글로벌경영학회 2011 글로벌경영학회지 Vol.8 No.2

本研究以长春市制造业4家公司的286名员工为有效样本, 探讨了情绪智力对工作压力和工作倦怠的影响的问题。研究在理论背景的基础上提出了研究假设和研究模型, 然后采用了结构方程建模(structural equation modeling: SEM)的方法, 验证了事先提出的研究假设。结果表明, 组织成员的情绪智力对工作压力有着显著负面影响, 对工作倦怠的两个维度(消极怠慢和成就感低落)有着显著负面影响, 工作压力对工作倦怠的三个维度(情绪衰竭、消极怠慢和成就赶低落)有着显著正面影响。从而得知, 工作压力在情绪智力和情绪衰竭的关系中起到完全中介效应;在情绪智力和工作倦怠的两个维度(消极怠慢和成就感低落)的关系中起到部分中介效应。 This study is based on empirical study includes 226 organization middle managements of 5 manufacturing companies in Chang Chun, China. This study performs experimental examination of the effect of Emotional Intelligence on the Job Stress and Job Burn-out. Based on theories’ background, this paper developed the study model and framed hypothesis. The data is analyzed by using structural equation modeling(SEM). The results showed that Job stress was a full mediator of the relationship between emotional intelligence and job burnout whereas Job stress partially mediated the relationship of emotional intelligence with cynicism and reduced personal accomplishment. This implied that the mechanism of the effects of emotional intelligence on emotional exhaustion was different from that of the influence of emotional intelligence on cynicism and reduced personal accomplishment.