The International Mouse Phenotyping Consortium (IMPC): a functional catalogue of the mammalian genome that informs conservation

Muñ,oz-Fuentes, Violeta,Cacheiro, Pilar,Meehan, Terrence F.,Aguilar-Pimentel, Juan Antonio,Brown, Steve D. M.,Flenniken, Ann M.,Flicek, Paul,Galli, Antonella,Mashhadi, Hamed Haseli,Hrabě,de Springer Netherlands 2018 Conservation genetics Vol.19 No.4

<P>The International Mouse Phenotyping Consortium (IMPC) is building a catalogue of mammalian gene function by producing and phenotyping a knockout mouse line for every protein-coding gene. To date, the IMPC has generated and characterised 5186 mutant lines. One-third of the lines have been found to be non-viable and over 300 new mouse models of human disease have been identified thus far. While current bioinformatics efforts are focused on translating results to better understand human disease processes, IMPC data also aids understanding genetic function and processes in other species. Here we show, using gorilla genomic data, how genes essential to development in mice can be used to help assess the potentially deleterious impact of gene variants in other species. This type of analyses could be used to select optimal breeders in endangered species to maintain or increase fitness and avoid variants associated to impaired-health phenotypes or loss-of-function mutations in genes of critical importance. We also show, using selected examples from various mammal species, how IMPC data can aid in the identification of candidate genes for studying a condition of interest, deliver information about the mechanisms involved, or support predictions for the function of genes that may play a role in adaptation. With genotyping costs decreasing and the continued improvements of bioinformatics tools, the analyses we demonstrate can be routinely applied.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1007/s10592-018-1072-9) contains supplementary material, which is available to authorized users.</P>

Therapeutic Effects of Resiniferatoxin Related with Immunological Responses for Intestinal Inflammation in Trichinellosis

José Luis Muñoz-Carrillo,José Luis Muñoz-López,José Jesús Muñoz-Escobedo,Claudia Maldonado-Tapia,Oscar Gutiérrez-Coronado,Juan Francisco Contreras-Cordero,María Alejandra Moreno-García 대한기생충학ㆍ열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.6

The immune response against Trichinella spiralis at the intestinal level depends on the CD4+ T cells, which can both suppress or promote the inflammatory response through the synthesis of diverse cytokines. During the intestinal phase, the immune response is mixed (Th1/Th2) with the initial predominance of the Th1 response and the subsequent domination of Th2 response, which favor the development of intestinal pathology. In this context, the glucocorticoids (GC) are the pharmacotherapy for the intestinal inflammatory response in trichinellosis. However, its therapeutic use is limited, since studies have shown that treatment with GC suppresses the host immune system, favoring T. spiralis infection. In the search for novel pharmacological strategies that inhibit the Th1 immune response (proinflammatory) and assist the host against T. spiralis infection, recent studies showed that resiniferatoxin (RTX) had anti-inflammatory activity, which decreased the serum levels of IL-12, INF-γ, IL-1β, TNF-α, NO, and PGE2, as well the number of eosinophils in the blood, associated with decreased intestinal pathology and muscle parasite burden. These researches demonstrate that RTX is capable to inhibit the production of Th1 cytokines, contributing to the defense against T. spiralis infection, which places it as a new potential drug modulator of the immune response.

Antithrombotic Study and Identification of Metabolites in Leaf Extracts of Chaya [Cnidoscolus aconitifolius (Mill.) I.M. Johnst.]

Juan Pablo Quintal-Martínez,Irma Guadalupe Quintal-Ortiz,Ligia Gabriela Alonzo-Salomón,David Muñoz-Rodríguez,Maira Rubi Segura-Campos 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.12

In Mexico, Cnidoscolus aconitifolius (chaya) has been used to treat cardiovascular diseases (CVD). Because CVD are the number one cause of mortality, chaya use has become a health strategy. The aim of this study was to evaluate the antithrombotic activity and identify the metabolites in the most active extract. Aqueous (Aq), ethanolic (EtOH), acetonic (An), ethyl acetate (AcOEt), diethyl ether (Et2O), and hexanic (Hx) extracts were obtained. Platelet aggregation, phospholipase A2, prothrombin time (PT), activated partial thromboplastin time (aPTT), and clot lysis were evaluated. Metabolites were identified by gas chromatography–mass spectrometry (GC-MS). EtOH showed the greatest inhibition of platelet aggregation and phospholipase A2. Ac had the greatest effect on PT and aPTT. AcOEt had the greatest effect on clot lysis. EtOH, with the highest potential, was analyzed by GC-MS; fatty acids and triterpenes were identified. Thus, EtOH showed greater antiplatelet activity and other extracts showed moderate activity. This is a preliminary antithrombotic study. Future research will allow the development of nutraceuticals or functional ingredients for the prevention and treatment of thrombosis.

Stroke Severity Score based on Six Signs and Symptoms The 6S Score: A Simple Tool for Assessing Stroke Severity and In-hospital Mortality

Juan Manual Racosta,Federico Di Guglielmo,Francisco Ricardo Klein,Patricia Mariana Riccio,Francisco Muñoz Giacomelli,María Eugenia González Toledo,Fátima Pagani Cassará,Agustina Tamargo,Matías Delfitt 대한뇌졸중학회 2014 Journal of stroke Vol.16 No.3

Background and Purpose Ascertaining stroke severity and predicting risk of in-hospital mortality is crucial to advise patients and families about medical decisions. We developed and tested the validity of a new stroke score, the 6S Score (Stroke Severity Score based on Six Signs and Symptoms), for quantifying ischemic stroke severity and predicting in-hospital mortality. Methods We prospectively assessed 210 consecutive acute ischemic stroke patients. The cohort was further divided into a derivation (n=120) and a validation (n=90) sample. From a total of 10 stroke signs and symptoms, we selected those with likelihood ratio’s P<0.005. We tested the validity of the score for predicting in-hospital mortality by using receiver operating characteristic curves. We used a scatterplot and the Spearman’s test to evaluate the correlation between the 6S Score and the National Institutes of Health Stroke Scale as a marker of stroke severity. We used principal component and exploratory factor analyses for assessing qualitative aspects of the 6S Score. Results The C statistic for in-hospital mortality was 0.82 for the 6S Score and 0.86 for the National Institutes of health Stroke Scale, respectively, with no significant differences between each other (P=0.79). The correlation between both scores was strong (Spearman’s rho 0.68, P<0.001). The factor analyses showed a good balance between left/right hemispheres and anterior/posterior circulations. Conclusions The 6S Score may constitute a tool for easily assessing stroke severity and predicting stroke mortality. Further research is needed for further assessing its external validity.

Development of nanostructure in ultra-fine anatase powders derived by the low temperature sol gel-solvothermal process

Oliver Muñiz-Serrato,Juan Serrato Rodríguez 한양대학교 세라믹연구소 2015 Journal of Ceramic Processing Research Vol.16 No.5

Ultra-fine crystalline anatase nano-powders of less than 6 nm particle size have been obtained at the early stage of crystallization using the one step low temperature non-aqueous sol gel-solvothermal treatment of the Ti(OBu)4 precursor. The solvothermal treatment is held at 110 o C for 10, 20, 40, 60 hours in the presence of 2-etoxiethanol and ethanol as solvent. Nanostructural development is followed by TEM, HRTEM, electron and X Ray diffraction. Particle coarsening by oriented attachment is evidenced by HRTEM observations and by particle growth kinetics during the solvothermal treatment.

Formation of flow coated high catalytic activity thin films from the low temperature sol-gel titanium butoxide precursor

Oliver Muñiz-Serrato,Juan Serrato Rodríguez 한양대학교 세라믹연구소 2018 Journal of Ceramic Processing Research Vol.19 No.4

The low temperature sol-gel approach is used in which the titanium butoxide (IV) precursor is hydrolyzed in the presence ofacetic and nitric acids to form a bidentate acetate complex (1538, 1429 cm−1 IR doublet). It is known that the role of the aceticacid as a chemical modifier controls the condensation and oligomerization reactions, but other effect of the complex acetateligand comprises the preferential crystallization of the titania into the anatase phase. In addition, it is found here, that thepresence of such a ligand at acidic pH values considerably impacts the nanostructure i.e. the formation of smooth hydrophobicanatase films containing fine non-aggregated nano-crystallites with auto-assembled micro pores. The resulting anatase-acetatenano-crystalline material is used to form thin films by the flow coating process. 4.2 nm size crystalline anatase and fullydispersed nanoparticles form a pattern of ultra-small crystallites as shown by TEM that are presumably responsible for theremarkable smooth and macro porous thin films with applications in photo-catalysis.

Trends in Scientific Literature on Atypical Antipsychotics in South Korea: A Bibliometric Study

Francisco López-Muñoz,Winston W. Shen,Raquel Moreno,Gabriel Rubio,Juan D. Molina,Concha Noriega,Miguel A. Pérez-Nieto,Lorena Huelves,Cecilio Álamo,배치운 대한신경정신의학회 2013 PSYCHIATRY INVESTIGATION Vol.10 No.1

Objective We have carried out a bibliometric study on the scientific publications in relation to atypical or second-generation antipsychotic drugs (SGAs) in South Korea. Methods With the EMBASE and MEDLINE databases, we selected those publications made in South Korea whose title included the descriptors atypic* (atypical*) antipsychotic*, second-generation antipsychotic*, clozapine, risperidone, olanzapine, ziprasidone, quetiapine, sertindole, aripiprazole, paliperidone, amisulpride, zotepine, asenapine, iloperidone, lurasidone, perospirone and blonanserin. We applied some bibliometric indicators of paper production and dispersion with Price's law and Bradford's law, respectively. We also calculated the participation index (PI) of the different countries, and correlated the bibliometric data with some social and health data from Korea (such as total per capita expenditure on health and gross domestic expenditure on research and development). Results We collected 326 original papers published between 1993 and 2011. Our results state fulfilment of fulfilled Price's law, with scientific production on SGAs showing exponential growth (correlation coefficient r=0.8978, as against an r=0.8149 after linear adjustment). The most widely studied drugs were risperidone (91 papers), aripiprazole (77), olanzapine (53), and clozapine (43). Division into Bradford zones yielded a nucleus occupied by the Progress in Neuro-Psychopharmacology and Biological Psychiatry (36 articles). A total of 86 different journals were published, with 4 of the first 10 used journals having an impact factor being greater than 4. Conclusion The publications on SGAs in South Korea have undergone exponential growth over the studied period, without evidence of reaching a saturation point.

Numerical investigation of truck aerodynamics on several classes of infrastructures

Alejandro Alonso-Estébanez,Juan J. del Coz Díaz,Felipe P. Álvarez Rabanal,Pablo Pascual-Muñoz,Paulino J. García Nieto 한국풍공학회 2018 Wind and Structures, An International Journal (WAS Vol.26 No.1

This paper describes the effect of different testing parameters (configuration of infrastructure and truck position on road) on truck aerodynamic coefficients under cross wind conditions, by means of a numerical approach known as Large Eddy Simulation (LES). In order to estimate the air flow behaviour around both the infrastructure and the truck, the filtered continuity and momentum equations along with the Smagorinsky–Lilly model were solved. A solution for these non-linear equations was approached through the finite volume method (FVM) and using temporal and spatial discretization schemes. As for the results, the aerodynamic coefficients acting on the truck model exhibited nearly constant values regardless of the Reynolds number. The flat ground is the infrastructure where the rollover coefficient acting on the truck model showed lowest values under cross wind conditions (yaw angle of 90), while the worst infrastructure studied for vehicle stability was an embankment with downward-slope on the leeward side. The position of the truck on the road and the value of embankment slope angle that minimizes the rollover coefficient were determined by successfully applying the Response Surface Methodology.

Aculturación Y Estilos de Vida de Los Migrantes Mexicanos en Estados Unidos

Sagrario Garay Villegas,Juan Martell Muñoz 부산외국어대학교 이베로아메리카연구소 2015 이베로아메리카 Vol.17 No.1

El flujo migratorio de mexicanos que se dirigen a Estados Unidos es un hecho conocido y documentado desde hace varias décadas. Las variaciones en las características de los migrantes y en su monto hace que cada vez se torne más complejo el estudio de este fenómeno, y al mismo tiempo permite que se deriven una serie de temas asociados con el desplazamiento, la adaptación de los migrantes en el país receptor y las implicaciones que se originan tanto en los migrantes como en su entorno. En particular en este artículo se tiene como objetivo principal mostrar el papel de la aculturación (vista a través del dominio del idioma inglés) en los estilos de vida de los migrantes mexicanos en Estados Unidos. Para llevar a cabo lo anterior se hace una discusión amplia sobre los procesos migratorios, la aculturación y los estilos de vida de los migrantes; además a partir de la Hispanic Health Care Survey 2007, se realiza una aproximación empírica de los estilos de vida de los migrantes mexicanos más y menos aculturizados. Palabras Clave: Migrantes, Aculturación, Estilos de Vida y Salud. The flow of Mexicans who go to the United States has been studied for several decades. The changes in the amount and the characteristics of the migrants makes more and more complex the study of this phenomenon and, at the same time, it allows to derive a number of issues associated with their displacement, the adaptation of migrants in the host country and the implications that arise both migrants and their context. In particular in this article is aimed to show the role of acculturation (view through the dominance of the English language) on lifestyles of Mexican migrants in the United States. To carry out the above we present a wide discussion on migratory processes, acculturation and the lifestyles of migrants. In addition, we use as source of data the Hispanic Health Care Survey 2007, like an empirical approximation to show the relation between acculturation, lifestyles and health of the Mexican migrants in United States. Key Words: Migrants, Acculturation, Lifestyles and Health

Biosorption of Heavy Metals from Acid Mine Drainage onto Biopolymers (Chitin and α (1,3) β-D-glucan) from Industrial Biowaste Exhausted Brewer’s Yeasts (Saccharomyces cerevisiae L.)

Fernando I. Ramírez-Paredes,Teresa Manzano-Muñoz,Juan C. Garcia-Prieto,Galina G. Zhadan,Valery L. Shnyrov,John F. Kennedy,Manuel G. Roig 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.6

A biosorption process has been developed for the bioremediation of heavy metal-contaminated acid drainages from Merladet and Faith open-cast mines,located in western Spain. The process is based on the physico-chemical properties for the adsorption, ion exchange, and complexation of metal ions by biopolymers (chitin and α (1,3) β-D-glucan) from industrial biowaste exhausted brewer's yeast (Saccharomyces cerevisiae L.). Firstly, the chemical composition (U, Mn, Al, Fe, Cu, Zn,and Ni) and the physico-chemical and ecological states of these acid mine drainages were characterised. Furthermore,the selectivity for Zn, Cu, Mn, Ni, and Al the first order kinetics and the performance of the metals biosorption process by exhausted brewer's yeast were evaluated with polluted acid synthetic waters and mine drainages. The biosorption equilibria were reached in 10 ~ 15 min following Langmuir type isotherms with higher affinity constants for metal-biosorbent binding for synthetic waters than for acid mine drainages. The efficiency of the process with real water samples was markedly lower for the case of Mn, and zero for Zn and Al. An antagonistic interference on the biosorption of a metal due to the presence of other metals is proposed. Finally, the ecotoxicity of the acid mine drainage was removed when it was incubated with brewer’s yeast trapped in polyurethane foam.