Inhibition of the fungal exopolysaccharides from the submerged liquid culture of Fomitopsis castanea mycelia against melanogenesis

Juhui JIN,Thi Thannh Hanh NGUYEN,Changmu KIM,Ilkyoon MOK,Doman KIM 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

Effects of green tea leaf, lotus leaf, and kimchi powders on quality characteristics of chicken liver sausages

( Juhui Choe ),( Gye-woong Kim ),( Hack-youn Kim ) 한국축산학회(구 한국동물자원과학회) 2019 한국축산학회지 Vol.61 No.1

Liver sausage is flavorful and highly nutritious. However, liver has a relatively short shelf life due to acceleration of oxidation in the presence of endogenous enzymes and metals. Powders derived from natural sources, including plants or fruits, are applied to meat products for inhibiting oxidation without adverse effects on their quality. Hence, this study investigated the effects of natural powders derived from green tea leaf (GTL), lotus leaf (LL), and kimchi (KC) on the quality and change in lipid oxidation and freshness of chicken liver sausages during two weeks of storage. Chicken liver sausages were manufactured with chicken breast (70%) and liver (20%), pork back fat (5%), iced water (5%), various additives, and GTL, LL, and KC [0 (control) or 1%]. They were processed in three batches. For determination of the quality characteristics of chicken liver sausages with various plant powders, pH, color, and texture properties were assessed. In addition, lipid oxidation and freshness using thiobarbituric acid reactive substances (TBARS) and total volatile basic nitrogen (TVBN) were analyzed at day 0 and week 2 of refrigerated storage. Higher values were obtained for pH and cooking yield in sausage samples with LL and KC powders than in samples with the other treatments. For a* values, the sausage samples with KC showed similar (p > 0.05) values, whilst others had significantly lower values than the control. The addition of the three powders to sausage samples induced an increase (p < 0.05) in hardness, gumminess, and chewiness. The addition of plant powders did not influence TBARS and TVBN of sausage samples at the initial stage. However, after two weeks of storage, significantly lower TBARS and TVBN values were observed, and the sausage with KC (p < 0.05) showed the lowest values of both TBARS and TVBN. The results showed the potential ability of the three powders to improve the quality and inhibit lipid oxidation in liver sausages. Particularly, the addition of KC did not adversely affect the a* values of sausage samples. The effects on sensory properties and inhibition mechanisms of GTL, LL, and KC in meat products should be further studied.

Space Composition and Landscape Characteristics around Village Forests in Wando-gun

Juhui Kim,Hyunmi Bae 전북대학교 휴양및경관계획연구소 2017 휴양및경관연구 (J East Asian Landscape Studies) Vol.11 No.1

The purpose of this study is to investigate the space composition and landscape structure around the village forests in five villages located on the coast of Wando-gun. The research focuses on field investigation and literature review. The results of this study are as follows In the case of village forests constructed for the protection of villages and cultivated areas in the coastal area of Wando-gun, which is the study area, it is confirmed that there are mountains behind the forests. Through the analysis of landscape elements, it was concluded that two types of layouts exist. Regarding the space composition around the village forest on the coast, it was found that there is a type A arranged in the order of sea → coast → village forest → village → cultivated land → rear mountain, and a type B arranged in the order the sea → coast → village forest → cultivated land → village → cultivated land → rear mountain. In the analysis of landscape characteristics, the elevation angle from the coast to the top of the mountain behind the village forest was measured. Accordingly, elevation angles of approximately 16°–17° in the type A arrangement and 7°–9° in type B arrangement were determined. From these results, it can be confirmed that the space composition and placement of the village forest with disaster prevention function vary according to the distance and elevation (changes in elevation angle) from the coast to the rear mountain.

Optimization of 1D ¹H Quantitative NMR (Nuclear Magnetic Resonance) Conditions for Polar Metabolites in Meat

Kim, Hyun Cheol,Ko, Yoon-Joo,Kim, Minsu,Choe, Juhui,Yong, Hae In,Jo, Cheorun Korean Society for Food Science of Animal Resource 2019 한국축산식품학회지 Vol.39 No.1

The objective of this study was to establish an optimized 1D $^1H$ quantitative nuclear magnetic resonance (qNMR) analytical method for analyzing polar metabolites in meat. Three extraction solutions [0.6 M perchloric acid, 10 mM phosphate buffer, water/methanol (1:1)], three reconstitution buffers [20 mM 3-morpholinopropane-1-sulfonic acid, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, phosphate buffer], and two pulse programs (zg30, noesypr1d) were evaluated. Extraction with 0.6 M perchloric acid and 20 mM phosphate resulted in a stable baseline and no additional overlap for quantifying polar metabolites in chicken breast. In qNMR analysis, zg30 pulse program (without water-suppression) showed smaller relative standard deviation (RSD) and faster running time than noesypr1d (water-suppression). High-performance liquid chromatography was compared with qNMR analyses to validate accuracy. The zg30 pulse program showed good accuracy and lower RSD. The optimized qNMR method was able to apply for beef and pork samples. Thus, an optimized 1D $^1H$ qNMR method for meat metabolomics was established.

Antimelanogenesis Effects of Fungal Exopolysaccharides Prepared from Submerged Culture of Fomitopsis castanea Mycelia

( Juhui Jin ),( Thi Thanh Hanh Nguyen ),( Changmu Kim ),( Doman Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8

Fungal exopolysaccharides are important natural products having diverse biological functions. In this study, exopolysaccharides from Fomitopsis castanea mycelia (FEPS) were prepared, and the highest mushroom tyrosinase inhibitory activity was found. FEPS were prepared from cultivation broth by ethanol precipitation method. The extraction yield and protein concentration of FEPS were 213.1 mg/l and 0.03%, respectively. FEPS inhibited mushroom tyrosinase with the half maximal inhibitory concentration (IC₅₀) of 16.5 mg/ml and dose-dependently inhibited cellular tyrosinase activity (63.9% at 50 μg/ml, and 83.3% at 100 μg/ml) in the cell-free extract of SK-MEL-5 human melanoma cell and α-melanocytestimulating hormone (α-MSH)-stimulated melanin formation in intact SK-MEL-5 human melanoma cell. The IC₅₀ of FEPS against NO production from RAW264.7 macrophage cells was 42.8 ± 0.64 μg/ml. By in vivo study using a zebrafish model, exposure of FEPS at 400 μg/ml to dechorionated zebrafish embryos for 18 h decreased the pigment density, compared to that without FEPS-treated control.

함정용 다기능 콘솔 내부 음향 시스템 설계에 관한 고찰

김주희(Juhui Kim) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.11

감성 파악 연구를 위한 멀티 모달 안경형 웨어러블 디바이스의 설계

박주희(Juhui Park),권장호(Jangho Kwon),김다혜(Da-Hye Kim),박완주(Wanjoo Park),김래현(Laehyun Kim) 한국HCI학회 2017 한국HCI학회 학술대회 Vol.2017 No.2

최근 정신건강이 대두되면서 사람의 감성을 파악할 수 있는 연구가 진행되고 있다. 본 연구에서는 실험실에서의 감성 파악을 위한 데이터가 아닌 실생활에서 편리하게 데이터를 얻을 수 있는 안경형 웨어러블 디바이스를 개발하고 데이터의 신뢰성을 평가하였다. 레퍼런스인 손에서 측정한 피부전도도 신호와 개발한 안경형 디바이스의 안면부 콧등 및 유양돌기에서 측정한 피부전도도 신호 사이의 상관관계가 높아 신뢰성이 높은 디바이스임을 확인하였다. 또한 안경형 웨어러블 디바이스는 감성파악을 하기 위한 데이터를 얻는 생체신호 센서와 카메라 렌즈 부분의 기구적자유도를 높게 설계하여 다양한 사용자의 두상에 최적화하였다. 기존의 감성평가의 대부분은 실험실의 세팅된 상황에서 이루어지지만 안경형 웨어러블 디바이스를 개발 및 사용하여 다양한 환경에서 안정적인 사용자 감성 추정이 가능하였다.

자계결합형 무선전력전송 시스템 내 자계결합계수의 산출에 대한 연구

김주희(Juhui Kim) 전력전자학회 2019 전력전자학술대회 논문집 Vol.2019 No.11

Culture-Free Detection of Bacterial Pathogens on Plasmonic Nanopillar Arrays Using Rapid Raman Mapping

Ko, Juhui,Park, Sung-Gyu,Lee, Sangyeop,Wang, Xiaokun,Mun, Chaewon,Kim, Sunho,Kim, Dong-Ho,Choo, Jaebum American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.8

<P>We utilized a fast Raman spectral mapping technique for fast detection of bacterial pathogens. Three-dimensional (3D) plasmonic nanopillar arrays were fabricated using the nanolithography-free process consisting of maskless Ar plasma treatment of a polyethylene terephthalate substrate and subsequent metal deposition. Bacterial pathogens were immobilized on the positively charged poly(L-lysine)-coated 3D plasmonic substrate through electrostatic interactions. Then, the bacterial surfaces were selectively labeled with antibody-conjugated surface-enhanced Raman scattering (SERS) nanotags, and Raman mapping images were collected and statistically analyzed for quantitative analysis of bacteria. Salmonella typhimurium was selected as a model pathogen bacterium to confirm the efficacy of our SERS imaging technique. Minimum number of Raman mapping points with statistical reliability was determined to reduce assay time. It was possible to get a statistically reliable standard calibration curve for 529 pixels (laser spot with 60 mu m interval), which required a total mapping time of 45 min to get a standard calibration curve for five different concentrations of bacteria in the 0 to 10(6) CFU/mL range. No amplification step was necessary for quantification because low-abundance target bacteria could be measured using the Raman spectral mapping technique. Therefore, this approach allows accurate quantification of bacterial pathogens without any culturing or enrichment process.</P>

사용자 맞춤형 여행코스 추천 애플리케이션

강주희 ( Juhui Kang ),김은경 ( Eungyeong Kim ),김석훈 ( Seokhoon Kim ) 한국정보처리학회 2017 한국정보처리학회 학술대회논문집 Vol.24 No.2

매년 여행을 즐기는 여행객들의 수가 꾸준히 증가하고 있으며, 이러한 추세는 해외여행 뿐 아니라 국내 여행에서도 나타나고 있다. 국내 여행을 즐기는 여행객 수의 증가는 매우 다양하고 복합적인 요인들에 의해 이루어지고 있는 것이 사실이나, 국내 여행객들의 절대 다수는 해외여행과는 달리 패키지 형태 보다는 자유여행 형태의 여행을 선호하고 있다. 이는 해외 여행지 대비 국내 여행지가 여행객들이 취득 및 분석할 수 있는 정보의 접근성이 훨씬 높고 정보의 양 역시 풍부하다는 것에서 기인한다고 할 수 있다. 그러나 이러한 정보 접근의 용이성 및 정보량의 풍요성은 오히려 자유여행을 즐기고자 하는 여행객들이 여행코스 및 숙소를 정하는데 많은 시간을 투자하게 되는 요인으로 작용하고 있다. 때문에 이러한 단점을 해결하고자 본 논문에서 제안하는 애플리케이션은 국내 여행객들이 편리하고 손쉽게 국내 여행을 즐길 수 있도록 여행코스 및 숙소를 지정할 수 있는 기능을 제공한다. 이를 위해, 제안하는 애플리케이션에서는 국내 여행과 관련된 다양한 정보들을 각종 포털 사이트와 SNS에서 수집하고, 이를 기반으로 사용자 선호 정보와의 매칭을 통해 맞춤형 여행 코스 제안 및 숙소 예약 기능을 제공한다. 이를 통해, 국내 여행을 즐기는 여행객들에게 편리함을 제공하고, 국내 여행객 수의 증가를 기대할 수 있다.