황색포도알균에 의한 급성 화농성 천골장골관절염 1예

문수연,신준범,김경엽,김석연,문수영,문송미,이미숙 대한감염학회 2007 감염과 화학요법 Vol.39 No.5

저자들은 특별한 위험 인자 없이 심한 허리 및 왼쪽 둔부 통증으로 내원하여 황색포도알균에 의한 균혈증이 진단된 젊은 여성에서 드문 감염질환인 화농성 천골장골관절염과 엉덩근 및 엉덩허리근 농양이 발생된 예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Pyogenic sacroiliits is a rare disease and the diagnosis is difficult because of unawareness of the disease and nonspecific symptoms and signs mimicking septic hip, iliopsoas muscle abscess and herniation of intervertebral disk. There are some predisposing factors related to the disease like trauma, pregnancy, intravenous drug abuse and infection of other systems such as skin, genitourinary system and heart. Staphylococcus aureus is the most common causative bacteria. Here we describe a case of 27-year-old woman presented with acute onset of back and left buttock pain. Bone scintigraphy revealed increased uptake at left sacroiliac joint and computed tomography of the pelvis showed left sacroiliitis with left iliacus and iliopsoas abscess. There was no history of usual predisposing conditions. S. aureus was grown from blood cultures and the patient improved with 2 weeks of intravenous cefazolin followed by 4 weeks of oral clindamycin treatment.

Development of a novel endolysin, PanLys.1, for the specific inhibition of Peptostreptococcus anaerobius

Joonbeom Moon,Hanbeen Kim,Dongseok Lee,Jakyeom Seo Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.8

Objective: The objective of this study was to develop a novel endolysin (PanLys.1) for the specific killing of the ruminal hyper-ammonia-producing bacterium Peptostreptococcus anaerobius (P. anaerobius). Methods: Whole genome sequences of P. anaerobius strains and related bacteriophages were collected from the National Center for Biotechnology Information database, and the candidate gene for PanLys.1 was isolated based on amino acid sequences and conserved domain database (CDD) analysis. The gene was overexpressed using a pET system in Escherichia coli BL21 (DE3). The lytic activity of PanLys.1 was evaluated under various conditions (dosage, pH, temperature, NaCl, and metal ions) to determine the optimal lytic activity conditions. Finally, the killing activity of PanLys.1 against P. anaerobius was confirmed using an in vitro rumen fermentation system. Results: CDD analysis showed that PanLys.1 has a modular design with a catalytic domain, amidase-2, at the N-terminal, and a cell wall binding domain, from the CW-7 superfamily, at the C-terminal. The lytic activity of PanLys.1 against P. anaerobius was the highest at pH 8.0 (p<0.05) and was maintained at 37℃ to 45℃, and 0 to 250 mM NaCl. The activity of PanLys.1 significantly decreased (p<0.05) after Mn²⁺ or Zn²⁺ treatment. The relative abundance of P. anaerobius did not decrease after administration PanLys.1 under in vitro rumen conditions. Conclusion: The application of PanLys.1 to modulate P. anaerobius in the rumen might not be feasible because its lytic activity was not observed in in vitro rumen system.

A Case of Streptococcus pyogenes Pneumonia with Rhabdomyolysis

Shin, Joonbeom,Moon, Soo-youn,Maeng, Chi Hoon,Lee, Mi Suk,Lee, Sangho,Lee, Hee Joo,Lee, Sang Oh 대한감염학회 2007 감염과 화학요법 Vol.39 No.5

We describe a case of Streptococcus pyogenes pneumonia in a 63-year-old man presenting with rhabdomyolysis and acute renal failure. The patient completely recovered following treatment with mechanical ventilation, continuous venovenous hemodiafiltration, and intravenous antimicrobial agents. 저자들은 제2형 당뇨병으로 진단 받고 인슐린으로 당뇨조절 중이던 68세 남자에서 횡문근융해증이 동반된 Streptococcus pyogenes에 의한 지역사회폐렴 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

Autophagy induced by AXL receptor tyrosine kinase alleviates acute liver injury via inhibition of NLRP3 inflammasome activation in mice

Han, Jihye,Bae, Joonbeom,Choi, Chang-Yong,Choi, Sang-Pil,Kang, Hyung-Sik,Jo, Eun-Kyeong,Park, Jongsun,Lee, Young Sik,Moon, Hyun-Seuk,Park, Chung-Gyu,Lee, Myung-Shik,Chun, Taehoon Informa UK (TaylorFrancis) 2016 AUTOPHAGY Vol.12 No.12

<P>Severe hepatic inflammation is a common cause of acute or chronic liver disease. Macrophages are one of the key mediators which regulate the progress of hepatic inflammation. Increasing evidence shows that the TAM (TYRO3, AXL and MERTK) family of RTKs (receptor tyrosine kinases), which is expressed in macrophages, alleviates inflammatory responses through a negative feedback loop. However, the functional contribution of each TAM family member to the progression of hepatic inflammation remains elusive. In this study, we explore the role of individual TAM family proteins during autophagy induction and evaluate their contribution to hepatic inflammation. Among the TAM family of RTKs, AXL (AXL receptor tyrosine kinase) only induces autophagy in macrophages after interaction with its ligand, GAS6 (growth arrest specific 6). Based on our results, autophosphorylation of 2 tyrosine residues (Tyr815 and Tyr860) in the cytoplasmic domain of AXL in mice is required for autophagy induction and AXL-mediated autophagy induction is dependent on MAPK (mitogen-activated protein kinase)14 activity. Furthermore, induction of AXL-mediated autophagy prevents CASP1 (caspase 1)-dependent IL1B (interleukin 1, ) and IL18 (interleukin 18) maturation by inhibiting NLRP3 (NLR family, pyrin domain containing 3) inflammasome activation. In agreement with these observations, axl(-/-) mice show more severe symptoms than do wild-type (Axl(+/+)) mice following acute hepatic injury induced by administration of lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). Hence, GAS6-AXL signaling-mediated autophagy induction in murine macrophages ameliorates hepatic inflammatory responses by inhibiting NLRP3 inflammasome activation.</P>

다발성 경화증 환자에서 발병된 청소년 류마티스 관절염 1예

문수영 ( Soo Young Moon ),이연아 ( Yeon Ah Lee ),신준범 ( Joonbeom Shin ),이상훈 ( Sang Hoon Lee ),홍승재 ( Seung Jae Hong ),양형인 ( Hyung In Yang ) 대한내과학회 2008 대한내과학회지 Vol.74 No.1

다발성 경화증은 중추신경계의 면역 이상에 따른 염증성 탈수초성 질환으로 병변이 발생하는 위치에 따라 다양한 신경계 증상으로 발현한다. 다발성 경화증은 다른 자가 면역질환을 동반하거나 전신 홍반 루푸스의 가족력이 있는 경우가 있으나, 다발성 경화증에 있어서 청소년 류마티스 관절염이 동반되는 경우는 매우 드물어 국내에서 보고된 바가 없다. 저자들은 다발성 경화증을 진단 받은 9세 여자 환자가 3년 후 다발성 관절통을 주소로 내원하여, 류마티스 인자가 양성인 청소년 류마티스 관절염으로 진단된 예를 경험하였기에 이를 보고하는 바이다. Multiple sclerosis (MS) is an immune-mediated, inflammatory demyelinating disease of the central nervous system. The coexistence of multiple sclerosis and rheumatoid arthritis is very rare. This rare association is interesting due to the overlapping pathophysiological similarities of T-cells and tumor necrosis factor-α (TNF-α) in both diseases. We report a case of a Korean 10-year-old girl with multiple sclerosis, who after 3 years developed a clinical and serological manifestation of juvenile rheumatoid arthritis. (Korean J Med 74:106-109, 2008)

Replacement of corn with rice grains did not alter growth performance and rumen fermentation in growing Hanwoo steers

Yang, Sungjae,Kim, Byeongwoo,Kim, Hanbeen,Moon, Joonbeom,Yoo, Daekyum,Baek, Youl-Chang,Lee, Seyoung,Seo, Jakyeom Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.2

Objective: This study was realized to evaluate the nutritional value of rice grains as a replacement for corn grains in the diet of growing Hanwoo steers. Methods: Two experimental diets were prepared: i) Corn total mixed ration (TMR) consisting of 20% corn grains and ii) Rice TMR consisting of 20% rice grains, in a dry matter (DM) basis. These treatments were used for in vitro rumen fermentation and in vivo growth trials. In the rumen fermentation experiment, the in vitro DM digestibility (IVDMD), in vitro crude protein digestibility (IVCPD), in vitro neutral detergent fiber digestibility, pH, ammonia nitrogen, and volatile fatty acids (VFA) were estimated at 48 h, and the gas production was measured at 3, 6, 12, 24, and 48 h. Twenty four growing Hanwoo steers (9 months old; body weight [BW]: 259±13 kg) were randomly divided into two treatment groups and the BW, dry matter intake (DMI), average daily gain (ADG), and feed conversion ratio (FCR) were measured. Results: The in vitro experiment showed that the IVDMD, IVCPD, and VFA production of the Rice TMR were higher than those of the Corn TMR (p<0.05). The growth trial showed no differences (p>0.05) in the final BW, ADG, DMI, and FCR between the two TMRs. Conclusion: The use of rice grains instead of corn grains did not exhibit any negative effects on the rumen fermentation or growth performance, thereby rice grains with a DM of less than 20% could be used as a starch source in the diet of growing steers.