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Sunhee Yoon,Jonguk Ju,Soonjo Kwon,전태준,김선민 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.1
Mechanical vibration is a key external stimulus of living organisms. In previous studies, mechanical vibration has been found to affect the proliferation and differentiation of cells. However, there have been few reports of similar effects at the organismal scale. In this study, we demonstrated the effect of mechanical vibration on the growth and activity of Caenorhabditis elegans in the embryonic stage. The group of worms that were exposed to mechanical vibration during the embryonic period grew faster than the control group of worms. The growth of all groups was compared by measuring body length. Furthermore, we investigated the mechanical vibration effects in two types of mutants: body wall mechanosensory-abnormal mutants (mec-4(e1339)) and head mechanosensory-defective mutants (trp-4(sy695)). The mec-4(e1339) mutant was not affected by mechanical vibration during growth. On the other hand, the trp-4(sy695) mutant exposed to mechanical vibration showed a difference in growth compared with the control group, similar to wild-type worms. Therefore, mechanical vibration during the early developmental period of C. elegans affects the growth and activity of worms. Additionally, we observed a relationship between the gastrulation period and the mechanosensory system development of the worms. This study will serve as the basis for analyzing the effects of mechanical vibration on the developmental stages of C. elegans.
Liu, Xiao-Min,An, Jonguk,Han, Hay Ju,Kim, Sun Ho,Lim, Chae Oh,Yun, Dae-Jin,Chung, Woo Sik Springer 2014 Plant cell reports Vol.33 No.12
<P>ZAT11, a Zinc Finger of Arabidopsis Thaliana 11, is a dual-function transcriptional regulator that positively regulates primary root growth but negatively regulates Ni (2+) tolerance. Zinc Finger of Arabidopsis Thaliana 11 (ZAT11) is a C2H2-type zinc finger protein that has been reported to function as an active transcriptional repressor. However, the biological function of ZAT11 remains unknown. Here we show that GFP-tagged ZAT11 is targeted to the nucleus. Analysis of plants expressing ZAT11 promoter-GUS showed that ZAT11 is highly expressed in roots and particularly in root tips. To identify the biological function of ZAT11, we constructed three independent lines of ZAT11 overexpressing transgenic plant (ZAT11 OE). ZAT11 OE enhanced the elongation of primary root but reduced the metal tolerance against nickel ion (Ni(2+)). The reduced Ni(2+) tolerance of ZAT11 OE was correlated with decreased accumulation of Ni(2+) in plants. The decreased accumulation of Ni(2+) in ZAT11 OE was caused by the reduced transcription of a vacuolar Ni(2+) transporter gene. Taken together, our results suggest that ZAT11 is a dual function transcriptional regulator that positively regulates primary root growth but negatively regulates Ni(2+) tolerance.</P>
Highly Reliable Amorphous In-Ga-Zn-O Thin-Film Transistors Through the Addition of Nitrogen Doping
Park, Kyung,Kim, Jong Heon,Sung, Taehoon,Park, Hyun-Woo,Baeck, Ju-Heyuck,Bae, Jonguk,Park, Kwon-Shik,Yoon, Sooyoung,Kang, Inbyeong,Chung, Kwun-Bum,Kim, Hyun-Suk,Kwon, Jang-Yeon Institute of Electrical and Electronics Engineers 2019 IEEE transactions on electron devices Vol.66 No.1
<P>The electrical properties and the device reliability under positive/negative bias stress with/without illumination regarding amorphous indium gallium zinc oxide (a-IGZO) thin-film transistors (TFTs) are evaluated as a function of the nitrogen partial pressure in the sputter deposition. Interestingly, it is easy to control the carrier concentration through the incorporation of nitrogen into IGZO, whereby the device performance is changed. In addition, when nitrogen is introduced during the conventional IGZO deposition, the device reliability of N-doped IGZO TFTs is considerably improved compared to that of undoped-IGZO TFTs due to the reduced amount of defects. It is also interesting that such an improvement of the device reliability regarding IGZO is easily obtainable through the simple addition of nitrogen to the conventional deposition process. It is, therefore, strongly believed that this simple nitrogen-doping process for IGZO is very effective regarding the achievement of highly durable devices, and it can be immediately applied to the current mass production of the high-performance displays for which the oxide semiconductor is used.</P>
Kim, Ho Soo,Park, Hyeong Cheol,Kim, Kyung Eun,Jung, Mi Soon,Han, Hay Ju,Kim, Sun Ho,Kwon, Young Sang,Bahk, Sunghwa,An, Jonguk,Bae, Dong Won,Yun, Dae-Jin,Kwak, Sang-Soo,Chung, Woo Sik Oxford University Press 2012 Nucleic acids research Vol.40 No.18
<P>Transcriptional repression of pathogen defense-related genes is essential for plant growth and development. Several proteins are known to be involved in the transcriptional regulation of plant defense responses. However, mechanisms by which expression of defense-related genes are regulated by repressor proteins are poorly characterized. Here, we describe the <I>in planta</I> function of CBNAC, a calmodulin-regulated NAC transcriptional repressor in <I>Arabidopsis</I>. A T-DNA insertional mutant (<I>cbnac1</I>) displayed enhanced resistance to a virulent strain of the bacterial pathogen <I>Pseudomonas syringae</I> DC3000 (<I>Pst</I>DC3000), whereas resistance was reduced in transgenic <I>CBNAC</I> overexpression lines. The observed changes in disease resistance were correlated with alterations in <I>pathogenesis-related protein 1</I> (<I>PR1</I>) gene expression. CBNAC bound directly to the <I>PR1</I> promoter. SNI1 (<I>suppressor of nonexpressor of PR genes1, inducible 1</I>) was identified as a CBNAC-binding protein. Basal resistance to <I>Pst</I>DC3000 and derepression of <I>PR1</I> expression was greater in the <I>cbnac1 sni1</I> double mutant than in either <I>cbnac1</I> or <I>sni1</I> mutants. SNI1 enhanced binding of CBNAC to its cognate <I>PR1</I> promoter element. CBNAC and SNI1 are hypothesized to work as repressor proteins in the cooperative suppression of plant basal defense.</P>
PZT 파우더 첨가에 따른 티타늄 파우더/폴리머 콘크리트 복합재료의 진동 특성 및 압축 물성 분석
박재현,김석룡,김경수,김건,김석호,이범주,정안목,안종욱,김선주,이시맥,유형민,Park, Jaehyun,Kim, Seok-Ryong,Kim, Kyoung-Soo,Kim, Geon,Kim, Seok-Ho,Lee, Beom-Joo,Jeong, Anmok,An, Jonguk,Kim, Seon Ju,Lee, Si-Maek,Yoo, Hyeong-Min 한국복합재료학회 2022 Composites research Vol.35 No.3
In this study, Ti powder/Polymer concrete composites were processed by adding the PZT powder, one of the piezoelectric materials, to improve the vibration damping effect of Polymer concrete. Ti powder was added at a constant ratio in order to maximize the vibration damping effect using the piezoelectric effect. Three types of composite material specimens were prepared: a specimen without PZT powder, specimens with 2.5 wt% and 5 wt% of PZT powder. The vibration characteristics and compression properties were analyzed for all specimens. As a result, it was confirmed that as the addition ratio of PZT powder increased, the Inertance value at the resonant frequency decreased due to the piezoelectric effect when the vibration generated from Ti powder/polymer concrete was transmitted. Especially, the Inertance value was decreased by about 19.3% compared to the specimen without PZT at the resonant frequency. The change in acceleration with time also significantly decreased as PZT powder was added, confirming the effect of PZT addition. In addition, through the compression strength test, it was found that the degree of deterioration in compression properties due to the addition of PZT up to 5 wt% was insignificant, and it was confirmed that the powder was evenly dispersed in the composites through the cross-sectional analysis of the specimen.