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Woo, Jongmin,Han, Dohyun,Wang, Joseph Injae,Park, Joonho,Kim, Hyunsoo,Kim, Youngsoo American Chemical Society 2017 Journal of proteome research Vol.16 No.9
<P>The development of systematic proteomic quantification techniques in systems biology research has enabled one to perform an in-depth analysis of cellular systems. We have developed a systematic proteomic approach that encompasses the spectrum from global to targeted analysis on a single platform. We have applied this technique to an activated microglia cell system to examine changes in the intracellular and extracellular proteomes. Microglia become activated when their homeostatic microenvironment is disrupted. There are varying degrees of microglial activation, and we chose to focus on the proinflammatory reactive state that is induced by exposure to such stimuli as lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma). Using an improved shotgun proteomics approach, we identified 5497 proteins in the whole-cell proteome and 4938 proteins in the secretome that were associated with the activation of BV2 mouse microglia by LPS or IFN-gamma. Of the differentially expressed proteins in stimulated microglia, we classified pathways that were related to immune-inflammatory responses and metabolism. Our label-free parallel reaction monitoring (PRM) approach made it possible to comprehensively measure the hyper-multiplex quantitative value of each protein by high resolution mass spectrometry. Over 450 peptides that corresponded to pathway proteins and direct or indirect interactors via the STRING database were quantified by label-free PRM in a single run. Moreover, we performed a longitudinal quantification of secreted proteins during microglial activation, in which neurotoxic molecules that mediate neuronal cell loss in the brain are released. These data suggest that latent pathways that are associated with neurodegenerative diseases can be discovered by constructing and analyzing a pathway network model of proteins. Furthermore, this systematic quantification platform has tremendous potential for applications in large-scale targeted analyses. The proteomics data for discovery and label-free PRM analysis have been deposited to the ProteomeXchange Consortium with identifiers <PXDO06558> and <PXDO06556>, respectively.</P>
Woo, Hyeonseok,Kim, Jongmin,Cho, Sangeun,Jo, Yongcheol,Roh, Cheong Hyun,Kim, Hyungsang,Hahn, Cheol-Koo,Im, Hyunsik The Korean Vacuum Society 2017 Applied Science and Convergence Technology Vol.26 No.3
An InGaN/GaN multiple quantum well (MQW) structure is grown on a GaN/sapphire template using a plasma-assisted molecular beam epitaxy (PA-MBE). The fluctuation of the quantum well thickness formed from roughly-grown InGaN layer results in a disordered photoluminescence (PL) spectrum. The surface morphologies of the InGaN layers with various In compositions are investigated by reflection high energy electron diffraction (RHEED) and atomic force microscopy (AFM). A blurred InGaN/GaN hetero-interface and the non-uniform QW size is confirmed by high resolution transmission electron microscopy (HR-TEM). Inhomogeneity of the quantum confinement results in a degradation of the quantum efficiency even though the InGaN layer has a uniform In composition.
Evaluation of Long-term Performance of Metal Seal Through Accelerated Test
Woo-seok Choi,Jongmin Lim,Yun-young Yang,Sang Soon Cho 한국방사성폐기물학회 2020 방사성폐기물학회지 Vol.18 No.2(E)
사용후핵연료를 저장하는 볼트체결 저장용기의 격납경계를 형성하는 주된 구성요소는 금속 밀봉재이다. 이러한 금속 밀봉 재는 열과 방사선에 의해 그 성질이 저하된다. 또한, 금속 밀봉재가 강한 열에 장기간 노출되면 크리프 현상이 발생한다. 이러한 크리프는 밀봉시스템에 응력 이완을 가져와서, 결국 밀봉재의 건전성을 해치게 된다. 이러한 응력 이완은 금속 밀봉재의 밀봉성능 저하로 이어지며, 저하의 정도에 따라 저장용기의 누설을 야기할 수 있다. 또한, 볼트 체결력의 감소도 밀봉성능 저하에 영향을 미친다. 본 논문에서는 금속 밀봉재의 격납건전성과 볼트체결력 감소를 평가하기 위해 수행한 가속화 시 험의 결과에 대하여 기술한다. 전 시험기간 동안 각 시편에서의 누설률, 볼트 변형률, 금속 밀봉재 주변 온도를 계측하여 분석하였고, 금속 밀봉재는 저장기간 50년 동안 격납건전성을 유지함을 입증하였다. 또한, 가속화 시험의 타당성에 대해서 기술하였다. Metal seals are the main components that establish the containment boundary in bolted casks, which store spent nuclear fuel. These seals are degraded by heat and radiation. In addition, creep occurs when the seals are exposed to intense heat for an extended period. This creep results in the stress relaxation of the seals, which consequently impairs the seal integrity. The stress relaxation can reduce the sealing performance of the metal seal, which can further cause leakage in the storage cask. Moreover, the reduction of bolt tension leads to sealing performance degradation. In this study, the results of high-temperature-accelerated tests were obtained to evaluate the containment integrity of metal seals and the decrease in bolt tension. During the tests, the leakage rate, bolt strain, and ambient temperature of the metal seals were measured and analyzed. The metal seals were found to maintain containment integrity for 50 years of storage. The validity of the acceleration test was also investigated.
( Jongmin Lee ),( Chang Dong Yeo ),( Sang Haak Lee ),( Jin Woo Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: A high proportion of patients in whom neutropenia had been complicated by pneumonia during neutropenia recovery are related to an increased risk of acute lung injury (ALI) and detrimental outcomes. The aim of the present study is to investigate whether neutrophil elastase (NE) inhibition is effective in murine LPS-induced ALI during neutropenia recovery and whether NE inhibition suppresses the activation of Mer signaling pathway. Methods: Cyclophosphamide was given to mice to induce neutropenia. Mice were administrated by intratracheal instillation of LPS seven days after cyclophosphamide administration. Sivelestat, neutrophuil elastase inhibitor, was given by intraperitoneal injection once daily starting on day 0 and continuing until mice were sacrificed on day 5 (preventive group). In addition, sivelestat was given after, instead of before, LPS administration on day 2 (therapeutic group). Results: Sivelestat attenuated the lung edema and histopathological change of LPS-induced lung injury. The accumulation of neutrophils and the concentrations of TNF-a, IL-6 and MPO in BAL fiuids were effectively inhibited by sivelestat. The gene expression of Mer tyrosine kinase was increased by sivelestat treatment. Conclusions: Sivelestat significantly attenuated LPS induced ALI during neutropenia recovery. These findings suggest NE inhibition could be a promising target alleviating lung infiammation without increasing susceptibility to infection in ALI/ARDS during neutropenia recovery.
Jongmin Sim*,Hannah Lois Kangleon-Tan*,Ji Young You,Eun-Shin Lee,Haemin Lee,Sun Moon Yang,Min-Ki Seong,Eun Hwa Park,Seok Jin Nam,박민호,Seokwon Lee,Woo-Chan Park,Rogelio G. Kangleon Jr,Crisostomo B. Dy,S 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.103 No.6
Purpose: Although adjuvant chemotherapy (CTx) is still recommended for high-risk patients with hormone receptorpositive and human epidermal receptor (HER)-2-negative breast cancer, recent studies found that selected patients with low disease burden may be spared from CTx and receive hormonal treatment (HT) alone. This study aims to evaluate the trends of treatment (CTx + HT vs. HT alone) in Korea and to assess the impact on overall survival (OS) according to treatment pattern. Methods: The Korean Breast Cancer Society Registry was queried (2000 to 2018) for women with pT1-2N0-1 hormone receptor-positive and HER2-negative disease who underwent surgery and adjuvant systemic treatment (CTx and HT). Clinicopathologic factors, change in pattern of treatment over time, and OS for each treatment option were analyzed. Results: A total of 40,938 women were included in the study; 20,880 (51.0%) received CTx + HT, while 20,058 (49.0%) received HT only. In recent years, there has been a steady increase in the use of HT alone, from 21.0% (2000) to 64.6% (2018). In Cox regression analysis, age, type of breast and axillary operations, T and N stages, body mass index, histologic grade, and presence of lymphovascular invasion were prognostic indicators for OS. There was no significant difference between CTx + HT and HT alone in terms of OS (P = 0.126). Conclusion: Over the years, there has been a shift from CTx + HT to HT alone without a significant difference in OS. Therefore, HT alone could be a safe treatment option in selected patients, even those with T2N1 disease.