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Peptide-Directed Photo-Cross-Linking for Site-Specific Conjugation of IgG
Park, Jisoo,Lee, Yumi,Ko, Byoung Joon,Yoo, Tae Hyeon American Chemical Society 2018 Bioconjugate chemistry Vol.29 No.10
<P>Conjugation of antibody has expanded its applications in therapeutics and diagnostics, and various methods have been developed based on chemical or enzymatic reactions. However, the majority of them have focused on synthetic molecules such as small molecules, nucleic acids, or synthetic materials, but site-specific conjugation of antibody with protein cargo has rarely been demonstrated. In this Communication, we report a PEptide-DIrected Photo-cross-linking (PEDIP) reaction for site-specific conjugation of IgG with protein using an Fc-binding peptide and a photoreactive amino acid analogue, and demonstrate this method by developing an immunotoxin composed of a Her2-targeting IgG (trastuzumab) and an engineered <I>Pseudomonas</I> exotoxin A (PE24). The ADP-ribosylation of eukaryotic elongation factor-2 by the bacterial toxin inhibits the ribosomal translation of protein, and the trastuzumab-PE24 conjugate exhibited the cytotoxicity toward Her2-overexpressing cell lines. The PEDIP reaction can also be applied for many other types of cargo with slight modifications of the method.</P> [FIG OMISSION]</BR>
Speech Separation through Audio-Visual Learning: A Survey
Jisoo Park,Junseok Kwon 대한전자공학회 2023 대한전자공학회 학술대회 Vol.2023 No.6
Audio-visual learning has gained significant attention in the field of deep learning, as it seeks to leverage the relationship between audio and visual modalities to improve audio-based tasks. Speech separation is a significant aspect of audio-visual learning that aims to address the cocktail party problem, where multiple sound sources overlap, making it challenging to isolate individual speech signals. In this survey paper, we provide an overview of the recent advances in audio-visual speech separation techniques and review the commonly used datasets in this area. We also identify the remaining challenges and discuss potential approaches for future research.
Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
Park, Jisoo,Lee, Hyunji,Tran, Quangdon,Mun, Kisun,Kim, Dohoon,Hong, Youngeun,Kwon, So Hee,Brazil, Derek,Park, Jongsun,Kim, Seon-Hwan Korean Society of ToxicologyKorea Environmental Mu 2017 Toxicological Research Vol.33 No.1
Transmembrane protein 39A (TMEM39A) belongs to the TMEM39 family. TMEM39A gene is a susceptibility locus for multiple sclerosis. In addition, TMEM39A seems to be implicated in systemic lupus erythematosus. However, any possible involvement of TMEM39A in cancer remains largely unknown. In the present report, we provide evidence that TMEM39A may play a role in brain tumors. Western blotting using an anti-TMEM39A antibody indicated that TMEM39A was overexpressed in glioblastoma cell lines, including U87-MG and U251-MG. Deep-sequencing transcriptomic profiling of U87-MG and U251-MG cells revealed that TMEM39A transcripts were upregulated in such cells compared with those of the cerebral cortex. Confocal microscopic analysis of U251-MG cells stained with anti-TMEM39A antibody showed that TMEM39A was located in dot-like structures lying close to the nucleus. TMEM39A probably located to mitochondria or to endosomes. Immunohistochemical analysis of glioma tissue specimens indicated that TMEM39A was markedly upregulated in such samples. Bioinformatic analysis of the Rembrandt knowledge base also supported upregulation of TMEM39A mRNA levels in glioma patients. Together, the results afford strong evidence that TMEM39A is upregulated in glioma cell lines and glioma tissue specimens. Therefore, TMEM39A may serve as a novel diagnostic marker of, and a therapeutic target for, gliomas and other cancers.