Recombinant Human Thioredoxin-1 Protects Macrophages from Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation and Cell Apoptosis

Zhang, Hui,Liu, Qi,Lin, Jia-Le,Wang, Yu,Zhang, Ruo-Xi,Hou, Jing-Bo,Yu, Bo The Korean Society of Applied Pharmacology 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.2

Oxidized low-density lipoprotein (ox-LDL)-induced macrophage foam cell formation and apoptosis play critical roles in the pathogenesis of atherosclerosis. Thioredoxin-1 (Trx) is an antioxidant that potently protects various cells from oxidative stress-induced cell death. However, the protective effect of Trx on ox-LDL-induced macrophage foam cell formation and apoptosis has not been studied. This study aims to investigate the effect of recombinant human Trx (rhTrx) on ox-LDL-stimulated RAW264.7 macrophages and elucidate the possible mechanisms. RhTrx significantly inhibited ox-LDL-induced cholesterol accumulation and apoptosis in RAW264.7 macrophages. RhTrx also suppressed the ox-LDL-induced overproduction of lectin-like oxidized LDL receptor (LOX-1), Bax and activated caspase-3, but it increased the expression of Bcl-2. In addition, rhTrx markedly inhibited the ox-LDL-induced production of intracellular reactive oxygen species (ROS) and phosphorylation of p38 mitogen-activated protein kinases (MAPK). Furthermore, anisomycin (a p38 MAPK activator) abolished the protective effect of rhTrx on ox-LDL-stimulated RAW264.7 cells, and SB203580 (a p38 MAPK inhibitor) exerted a similar effect as rhTrx. Collectively, these findings indicate that rhTrx suppresses ox-LDL-stimulated foam cell formation and macrophage apoptosis by inhibiting ROS generation, p38 MAPK activation and LOX-1 expression. Therefore, we propose that rhTrx has therapeutic potential in the prevention and treatment of atherosclerosis.

Trajectory Tracking Control of Nonholonomic Wheeled Mobile Robots Using Model Predictive Control Subjected to Lyapunov-based Input Constraints

Jing-Jun Zhang,Rui-Zhen Gao,Zhi-Li Fang,Zhong-Qi Zhang,Shao-Bo Zhang 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.5

This paper studies the trajectory tracking control for the nonholonomic wheeled mobile robot (WMR) based on model predictive control (MPC). Concerning overcome stable problem occurred from optimization control of MPC, we develop an MPC controller subjected to Lyapunov-based input constraints (LMPC) to solve the trajectory tracking problem for the nonholonomic WMR. The Lyapunov stable characteristic of the feedback linearization (FL) is utilized to ensure the stability of the control system. The recursive feasibility of input and stability of the control system based on LMPC is analyzed by the contraction constraint of FL. Besides, the paper also demonstrates the stability of the trajectory tracking control by the receding horizon optimized control solution embedded in LMPC. The simulation experiment results significantly verify the validity of the proposed LMPC approach in trajectory tracking.

Recombinant Human Thioredoxin-1 Protects Macrophages from Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation and Cell Apoptosis

( Hui Zhang ),( Qi Liu ),( Jia-le Lin ),( Yu Wang ),( Ruo-xi Zhang ),( Jing-bo Hou ),( Bo Yu ) 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.2

Oxidized low-density lipoprotein (ox-LDL)-induced macrophage foam cell formation and apoptosis play critical roles in the pathogenesis of atherosclerosis. Thioredoxin-1 (Trx) is an antioxidant that potently protects various cells from oxidative stress-induced cell death. However, the protective effect of Trx on ox-LDL-induced macrophage foam cell formation and apoptosis has not been studied. This study aims to investigate the effect of recombinant human Trx (rhTrx) on ox-LDL-stimulated RAW264.7 macrophages and elucidate the possible mechanisms. RhTrx significantly inhibited ox-LDL-induced cholesterol accumulation and apoptosis in RAW264.7 macrophages. RhTrx also suppressed the ox-LDL-induced overproduction of lectin-like oxidized LDL receptor (LOX- 1), Bax and activated caspase-3, but it increased the expression of Bcl-2. In addition, rhTrx markedly inhibited the ox-LDL-induced production of intracellular reactive oxygen species (ROS) and phosphorylation of p38 mitogen-activated protein kinases (MAPK). Furthermore, anisomycin (a p38 MAPK activator) abolished the protective effect of rhTrx on ox-LDL-stimulated RAW264.7 cells, and SB203580 (a p38 MAPK inhibitor) exerted a similar effect as rhTrx. Collectively, these findings indicate that rhTrx suppresses ox-LDL-stimulated foam cell formation and macrophage apoptosis by inhibiting ROS generation, p38 MAPK activation and LOX-1 expression. Therefore, we propose that rhTrx has therapeutic potential in the prevention and treatment of atherosclerosis.

Soluble expression, purification and the role of C-terminal glycine residues in scorpion toxin BmK AGP-SYPU2

( Rong Zhang ),( Yong Cui ),( Xi Zhang ),( Zhuo Yang ),( Yong Shan Zhao ),( Yong Bo Song ),( Chun Fu Wu ),( Jing Hai Zhang ) 생화학분자생물학회 2010 BMB Reports Vol.43 No.12

The existence of glycine residues in long-chain scorpion toxins has been well documented. However, their role as analgesics has not been evaluated. To address this issue, we investigated the functional role of glycines in the C-terminal end of Chinese-scorpion toxin from Buthus martensii Karsch (BmK AGP-SYPU2) using site-directed mutagenesis and analgesic activity assays. Recombinant BmK AGP-SYPU2 and its mutants were efficiently expressed in E. coli and purified to homogeneity using immobilized metal ion affinity chromatography (IMAC) and cation exchange chromatography. The mouse-twisting test was used to detect the analgesic activity of BmK AGP-SYPU2 and its mutants. As a result, we identified glycines at the C-terminal end that, when altered, significantly affected analgesic activity. Also, Mut6566 was significantly decreased compared to BmK AGP-SYPU2. These data indicate that the glycines at the C-terminal end are important for the analgesic activity of BmK AGP-SYPU2. [BMB reports 2010; 43(12): 801-806]

Research of Evaluation System of English Autonomous Learning Ability Based on BP Neural Network

Bo Du,Yan Zhang,Jing Zhang,Dongming Li 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.12

This paper focuses on the application of BP neural network, taking an evaluation system of English autonomous learning ability as an example. Based on the full analysis the inscape of the system, the recorded data that is related with Web server log in a process of students' online learning has been collected and analyzed by using data mining technology. With BP neural network pattern, an evaluation model of autonomous learning ability based on BP neural network has been founded on the data collected. The experimental results show that the evaluation results of model are in general accord with those of experts: the highest accuracy was 100%, the lowest is 91.7%, and the average is 94.6%; therefore, it is feasible and effective to apply the BP neural network to the evaluation of autonomous learning ability.

ZnO Nanowires/ CdS Nanorods Structure Grown on Silica Micropillars Array for Photosensitive Application

Jing Liu,Yuanze Xu,Xiaoxiao Liang,Mingming Yan,Bo Wang,Tianchong Zhang,Zhen Hong,Futing Yi 대한금속·재료학회 2021 ELECTRONIC MATERIALS LETTERS Vol.17 No.6

Utilizing silica micropillar arrays as substrate, a kind of Zinc oxide (ZnO) nanowires/Cadmium sulfi de (CdS) nanopillars heterojunctionstructure is prepared successfully by twice hydrothermal reaction method. This ZnO nanowires/CdS nanorods/silicamicropillars structure is tree-liked morphology from the SEM and TEM images, and both the ZnO nanowires and CdS nanorodsare well crystallinity on the silica surface from the X-ray diff raction curves. The CdS nanorods/silica micropillars structure has alow refl ectivity to the incoming light for wavelength from 200 to 800 nm. After the ZnO nanowires growing, the refl ectivity oftree-liked structure is much lower than that without ZnO nanowires especially for the wavelength less than 350 nm. The resistanceresponse of the CdS nanorods/silica micropillars based photoresistors with and without ZnO nanowires are tested. And theresults reveal that the ZnO nanowires covering can improve the response of the CdS nanorods/silica micropillars photoresistors. The best photosensitivity response of the ZnO nanowires/CdS nanorods/silica micropillars photoresistor for 450 nm wavelengthlight with 5 mW/cm 2 illumination can achieve to 23.3, while that for CdS nanorods/silica micropillars photoresistors is 18.8.

Development of a Novel FES Control System Based on Treadmill Motor Current Variation for Gait Rehabilitation of Hemiplegic Patients after Stroke

Jing Ye,Yasutaka Nakashima,Takao Watanabe,Masatoshi Seki,Bo Zhang,Quanquan Liu,Yuki Yokoo,Yo Kobayashi,Qixin Cao,Masakatsu G. Fujie 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10

In consideration of a large proportion of stroke survivors with persistent deficits, current interventions have limited ability to restore normal motor function. We propose a gait training system for gait rehabilitation of hemiplegic patients with convalescent stroke. It consists of a functional electrical stimulation (FES) device which is used to influence the gait pattern instantly to improve the quality of the gait, and a treadmill with two separated belts. The stimulus triggers from the FES could be controlled automatically by the subject`s gait phase determination, including stance phase and swing phase. Gait phase could be estimated and recognized through observing current value variation of the treadmill motor during the subject’s walking on the treadmill belts. In this paper, we have preliminarily tested the feasibility of the proposed method through hemiplegic simulation experiments. Also, Tibialis Anterior (TA) and quadriceps on the hemiplegic side were successfully stimulated by the expected FES stimuli during gait.

Effect of Land-use Types on Soil Organic Carbon and Particulate Organic Carbon in Ecotones of Agriculture and Animal Husbandry

Bo Deng,Jinge An,Jianguo Han,Fuyu Yang,Yingjun Zhang 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

In this paper, the effect of different land-use types on soil organic carbon (SOC) and Particulate Organic Carbon(POC) was studied by analyzing the soils in the meadow and conversing land of grassland to cropland in ecotones of agriculture and animal husbandry. It is shown t㏊t, compared with meadow, rotations of P/O and P/Cwill reduce SOC in 0-30㎝ soil layers by 47.54%-65.11% and 34.16-64.26% and POC by 16.90%-24.48% and 11.94%-20.29%, respectively. And SOC in meadow is more t㏊n in conversing land of grassland to cropland (P<0.05). Conversing grassland to cropland in ecotones of agriculture and animal husbandry will decrease the contents of SOC and POC.

Urchin-like alkaline nickel–cobalt carbonate derived Ni3S4/Co3S4 nanoparticles anchored on rGO for lithium/sodium-ion batteries with enhanced capacity

Bo-Han Zhang,Yu-Qian Wang,Fen Gao,Hua-Ying Wang,Wan-Xin Wen,Yun-Lei Hou,Jing-Zhou Chen,Dong-Lin Zhao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

Transition metal sulfides (TMS) have emerged as promising anode materials for lithium and sodium ionbatteries due to their high theoretical capacity and low cost. However, the severe volume expansion duringthe conversion reaction causes rapid collapse inevitability when used as anode material. Herein, wepertinently fabricated nanoscale Ni3S4/Co3S4 with uniform particle size homogeneously dispersedbetween pleated graphene (Ni3S4/Co3S4@rGO) through facile hydrothermal assisted by cationic surfactants. The construction of 3D nanocomposites significantly shortens ion migration distances and enricheselectronic pathways, accelerating charge transfer kinetics while maintaining good stability. Uniformlydistributed bimetallic sulfides and rGO limit each other’s aggregation, allowing the excellent structuralproperties of the material to be maintained over time. Thus, the Ni3S4-Co3S4@rGO electrodes deliver ahigh reversible capacity of 972.5 mAh/g upon 200 cycles at 100 mA g1 in Lithium-ion battery and487.5 mAh/g upon 150 cycles at 100 mA g1 in Sodium-ion battery, as well as excellent rate capacityin both. These excellent properties show the potential for expansion of this simple synthetic methodin the field of alkali metal batteries.

Long noncoding RNA UCA1 inhibits ischaemia/reperfusion injury induced cardiomyocytes apoptosis via suppression of endoplasmic reticulum stress

Jing Chen,Qi Hu,Bo‑fang Zhang,Xiao‑pei Liu,Shuo Yang,Hong Jiang 한국유전학회 2019 Genes & Genomics Vol.41 No.7

Background Ischemia heart disease is one of the major causes of death worldwide which often associated with tissue infarction and limit the recovery of function. Multiple factors involved in the I/R-induced cardiomyocyte dysfunction which were consistent with a role of oxidative stress and altered endothelium-dependent responses. However, the pathogenic mechanisms in I/R injury remain unclear. Materials and methods The H9C2 cells were in the ischaemia/reperfusion (I/R) condition. After I/R, the cells were transfected with or without adenovirus-urothelial carcinoma associated 1(Ad-UCA1). Then qRT-PCR analysis was performed to quantify mRNA expression of different treatment groups. Cell apoptosis rate was assessed using flow cytometry and ER stress biomarker expression were measured by immunoblotting. Intracellular and mitochondrial ROS generation were assayed by fluorescence microscope after staining with the DCFDA or MitoSOX. Results I/R conditions trigger lncRNAs UCA1 expression, cellular and mitochondria ROS production, resulting in cell apoptosis through the induction of oxidative and ER stress. Overexpression of UCA1 protects H9C2 cells from I/R-induced ER stress and cell apoptosis. Moreover, UCA1 might be a potential regulator in the protective effect of I/R‑induced oxidative stress and mitochondria dysfunction. Subsequently, ER stress inhibitor attenuated the effect of siUCA1 induced injury in H9C2 cells. Conclusion The expression of UCA1 against I/R induced oxidative stress and mitochondria dysfunction via suppression of endoplasmic reticulum stress. UCA1 might be a biomarker to improved diagnosis of I/R injury.