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Ji, Hong,Wang, Jianfa,Liu, Juxiong,Guo, Jingru,Wang, Zhongwei,Zhang, Xu,Guo, Li,Yang, Huanmin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3
Zi geese (Anser anser domestica) belong to the white geese and are excellent layers with a superior feed-to-egg conversion ratio. Quantitative gene expression analysis, such as Real-time qRT-PCR, will provide a good understanding of ovarian function during egg-laying and consequently improve egg production. However, we still don't know what reference genes in geese, which show stable expression, should be used for such quantitative analysis. In order to reveal such reference genes, the stability of seven genes were tested in five tissues of Zi geese. Methodology/Principal Findings: The relative transcription levels of genes encoding hypoxanthine guanine phosphoribosyl transferase 1 (HPRT1), ${\beta}$-actin (ACTB), ${\beta}$-tubulin (TUB), glyceraldehyde-3-phosphate-dehydrogenase (GADPH), succinate dehydrogenase flavoprotein (SDH), 28S rRNA (28S) and 18S rRNA (18S) have been quantified in heart, liver, kidney, muscle and ovary in Zi geese respectively at different developmental stages (1 d, 2, 4, 6 and 8 months). The expression stability of these genes was analyzed using geNorm, NormFinder and BestKeeper software. Conclusions: The expression of 28S in heart, GAPDH in liver and ovary, ACTB in kidney and HPRT1 in muscle are the most stable genes as identified by the three different analysis methods. Thus, these genes are recommended for use as candidate reference genes to compare mRNA transcription in various developmental stages of geese.
( Ji Hong ),( Peng Zhang ),( I Na Yoon ),( Jae Sam Hwang ),( Jin Ku Kang ),( Ho Kim ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.4
Clostridium difficile, which causes pseudomembranous colitis, releases toxin A and toxin B. These toxins are considered to be the main causative agents for the disease pathogenesis, and their expression is associated with a marked increase of apoptosis in mucosal epithelial cells. Colonic epithelial cells are believed to form a physical barrier between the lumen and the submucosa, and abnormally increased mucosal epithelial cell apoptosis is considered to be an initial step in gut inflammation responses. Therefore, one approach to treating pseudomembranous colitis would be to develop agents that block the mucosal epithelial cell apoptosis caused by toxin A, thus restoring barrier function and curing inflammatory responses in the gut. We recently isolated an antimicrobial peptide, Periplanetasin-2 (Peri-2, YPCKLNLKLGKVPFH) from the American cockroach, whose extracts have shown great potential for clinical use. Here, we assessed whether Peri-2 could inhibit the cell toxicity and inflammation caused by C. difficile toxin A. Indeed, in human colonocyte HT29 cells, Peri-2 inhibited the toxin A-induced decrease in cell proliferation and ameliorated the cell apoptosis induced by this toxin. Moreover, in the toxin A-induced mouse enteritis model, Peri-2 blocked the mucosal disruption and inflammatory response caused by toxin A. These results suggest that the American cockroach peptide Peri-2 could be a possible drug candidate for addressing the pseudomembranous colitis caused by C. difficile toxin A.
Cholesterol Granuloma in the Mandible ; A Case Report
Jihong Kim,Seong Doo Hong,Jae lL Lee,Sam Pyo Hong 대한구강악안면병리학회 2007 대한구강악안면병리학회지 Vol.31 No.2
Cholesterol granuloma(CG) occurs frequently in association with chronic middle ear diseases. pa rti cul arly dis eases in the mastoid antrum a nd ai r cells of the tempora l bone. and much less frequent ly in paranasal sinuses, It occurs f1'eq uentJy seconda1'Y to massive hemor1'hage of oral and pal'aol'aJ cysts, Howeve1'. It has nevel' been I'eported to OCCUI' solely wi thout any association of pl'eexisting lesion in the mandibJe We expel'ienced development of unusuaJ cholestel'ol granuloma in the mandible, Seventy year old female pl'e sented diffuse ha1'd swelling on the left mandibular a rea with Iymphadenopathy in the left cer vical Iymph node Radi og1'aphic exarnination showed a well circumsc ribed multi locular radiolucency resembling soap bubble appeara nce with tooth di s placement and root resorption‘ leading to the radiogrphic imp1'ession of dentigerous cyst 0 1' 。dontogenic cys t or amelobJas toma, CT showed bucco-lingua lly undul ating expansi le lesion with co rticated ma l'gin from the left posterior mandibular bocly to the anterior ramus. including #46, #47 ancl #43. and the mass containing in the lesion showed s lightly lower a ttenuation than muscJe leading to the impression of ameloblastoma, The mass aftel' surgical excis ion composed of 3 sac like structures, measuring 4,1 cm, 1, 3cm ancl 1.4cm in diame ter respectively, One sac was t ightl y a ttached to the #46. l'eRembling dentigerous cyst, Mi croscopic examination showed a large numbel' of c h이 es te rol clefts in association with hemol'rhage, hemosiderin pigments and fO l'eign body giant cells, There was no evidence of cyst 0 1' other lesions, CG should be taken into diffe l'enti a l diagnosis in addition to odontogenic cysts and tumors when radiographica lly well ci rcumscribed multilocuJal' radiolucent lesion occurs