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RISS 인기검색어
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- 저자 : Jianliang Sun (검색결과 5 건)
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Screening nephroprotective compounds from cortex Moutan by mesangial cell extraction and UPLC
Min Sun,Limei Huang,Jianliang Zhu,Wenjie Bu,Jing Sun,Zhaohui Fang 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6
A method for screening nephroprotective compoundsin cortex Moutan, a common traditional Chinesemedicine (TCM) in treating diabetic nephropathy with renalmesangial cell extraction and ultra performance liquidchromatography technique was described in this paper. Wehypothesize that the compounds which bind to cell membranesunder pathological conditions may be the bioactivecompounds in TCMs. Mesangial cells were cultured inmedium containing 5 mM (physiological, NG) or 30 mM(pathological, HG) glucose for 48 h and then incubated withcortex Moutan extract. After the unbound substances werewashed off, the cell membrane-bound compounds weredissociated and concentrated by an SPE column. By comparingthe chromatograms of NG and HG cultured-cellextractions and cortex Moutan extract, three compoundsbound to both NG and HG-cultured mesangial cells wereidentified as paeoniflorin, pentagalloylglucose (PGG) andpaeonol. In vitro studies showed that paeoniflorin, PGG andpaeonol reduced the activity of nicotinamide-adenine dinucleotidephosphate oxidase (NADPH) activity, anddecreased the level of reactive oxygen species (ROS),transforming growth factor-b1 (TGF-b1) and fibronectin inhigh glucose cultured mesangial cells. The results indicatethat paeonol, paeoniflorin and PGG may be the nephroprotectivecompounds from cortex Moutan. This study isexpected to provide a more reliable and effective method forscreening bioactive compounds from the complex TCM systems.
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Multi-Modal Stress Characteristics Under Coupling Effect of Strip and Work Roll
Jinxing Cui,Yan Peng,Jianliang Sun,Boyang Li 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.10
In this paper, we investigate the rolling interface instability characteristics caused by the coupled motion of strip and the roll system. Based on the experimental test, the dynamical behaviors of the rolling mill under the motion of roll system and the strip show multi-modal vibration phenomenon, which are consistent with the variation of rolling interface force and energy parameters. Then, the dynamic geometric parameters of rolling interface and the dynamic stress models are established. Through the simulation analysis, it can be concluded the multi-modal stress state of the rolling interface is caused by the coupling motion of the strip and the roll system. The results show that the multi-modal stress of the rolling interface cause the rolling process instability, leading the "pulling " on the strip and "beating" on the work roll, which change the instability state of the rolling process. This study provides a theoretical basis for the study of multi-modal vibration characteristics of rolling mill.
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Slab analysis of large cylindrical shell rolling considering mixed friction
Suwen Chen,Hongmin Liu,Yan Peng,Jianliang Sun 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.11
Large cylindrical shell rolling is an advanced plastic-forming technique that has unequal speed and radius of both its upper and lowerrolls. A theoretical model for a large cylindrical shell rolling that is based on the slab method is proposed, in which the non-uniform normaland shear stresses that act on the vertical sides are considered. A mixed friction model of combined Coulomb and sticking friction isused to present interface friction, to improve the accuracy of the proposed model. The rolling pressure, rolling force, and torque as well asfriction stress can be rapidly and easily calculated through the proposed model at different rolling conditions. The predicted rolling forceis consistent with the experimental result. The research results provide valuable guidelines for both the design and optimization of rollingproduct, process, and equipment.
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Haiyun Chen,Jialong Liang,Xin Gu,Jiawen Zhou,Chunfeng Xie,Xianhui Lv,Rong Wang,Qing Liu,Zhiyuan Mao,Haijian Sun,Guoping Zuo,Dengshun Miao,Jianliang Jin 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-
To study whether TGF-β1/IL-11/MEK/ERK (TIME) signaling mediates senescence-associated pulmonary fibrosis (SAPF) in Bmi-1-deficient (Bmi-1−/−) mice and determines the major downstream mediator of Bmi-1 and crosstalk between p16INK4a and reactive oxygen species that regulates SAPF, phenotypes were compared among 7-week-old p16INK4a and Bmi-1 double-knockout, N-acetylcysteine (NAC)-treated Bmi-1−/−, Bmi-1−/−, and wild-type mice. Pulmonary fibroblasts and alveolar type II epithelial (AT2) cells were used for experiments. Human pulmonary tissues were tested for type Ι collagen, α-smooth muscle actin (α-SMA), p16INK4a, p53, p21, and TIME signaling by using enzyme-linked immunosorbent assay (ELISA). Our results demonstrated that Bmi-1 deficiency resulted in a shortened lifespan, ventilatory resistance, poor ventilatory compliance, and SAPF, including cell senescence, DNA damage, a senescence-associated secretory phenotype and collagen overdeposition that was mediated by the upregulation of TIME signaling. The signaling stimulated cell senescence, senescence-related secretion of TGF-β1 and IL-11 and production of collagen 1 by pulmonary fibroblasts and the epithelial-to-mesenchymal transition of AT2 cells. These processes were inhibited by anti-IL-11 or the MEK inhibitor PD98059. NAC treatment prolonged the lifespan and ameliorated pulmonary dysfunction and SAPF by downregulating TIME signaling more than p16INK4a deletion by inhibiting oxidative stress and DNA damage and promoting ubiquitinproteasome degradation of p16INK4a and p53. Cytoplasmic p16INK4a accumulation upregulated MEK/ERK signaling by inhibiting the translocation of pERK1/2 (Thr202/Tyr204) from the cytoplasm to the nucleus in senescent fibroblasts. The accumulation of collagen 1 and α-SMA in human lungs accompanied by cell senescence may be mediated by TIME signaling. Thus, this signaling in aging fibroblasts or AT2 cells could be a therapeutic target for preventing SAPF.
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