Periparturient stocking density affects lying and ruminating behavior and one-week-calf performance of Holstein cows

Jiang Mingming,Alugongo Gibson Maswayi,Xiao Jianxin,Li Congcong,Ma Yulin,Li Tingting,Cao Zhijun,Liu Dasen 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

Objective: This study aimed to investigate the effect of stocking density on the behavior, productivity, and metabolism of periparturient Holstein cows as well as calf performance. Methods: A total of 48 periparturient cows were randomly assigned into three groups at 28 days (±3 days) before their expected calving date. The stocking densities of the groups, relative to the standard cubicle and feed bunk number, were i) 80% (13 cows), ii) 100% (16 cows), and iii) 120% (19 cows). Lying and rumination behavior was recorded using electronic data loggers and HR-Tags from d -21 (“d-” means days before calving) until the calving date, d 0. Lying time was assessed to determine the diurnal total hours spent lying per day. Rumination time was averaged in 2 hours interval periods over 24 hours during the experimental period. Results: Cows in the 80% group spent more time lying and ruminating between d -21 and d -7 and tended to ruminate more between d -14 and d 0. Calcium levels tended to be higher for cows in the 80% group, no other observable differences were found in monitored blood parameters. Moreover, 3.5% fat corrected milk and energy corrected milk yields were higher in 80% group in the first month of lactation. No other observable differences were found in the yield and composition of colostrum and milk in the first 10 months of lactation. The growth and performance of calves in the first week of life was not affected by stocking density of the dams. Conclusion: We concluded that lower stocking density may increase lying and ruminating behavior of prepartum Holstein cows. However, this did not translate into improved productivity and metabolism. Objective: This study aimed to investigate the effect of stocking density on the behavior, productivity, and metabolism of periparturient Holstein cows as well as calf performance.Methods: A total of 48 periparturient cows were randomly assigned into three groups at 28 days (±3 days) before their expected calving date. The stocking densities of the groups, relative to the standard cubicle and feed bunk number, were i) 80% (13 cows), ii) 100% (16 cows), and iii) 120% (19 cows). Lying and rumination behavior was recorded using electronic data loggers and HR-Tags from d -21 (“d-” means days before calving) until the calving date, d 0. Lying time was assessed to determine the diurnal total hours spent lying per day. Rumination time was averaged in 2 hours interval periods over 24 hours during the experimental period.Results: Cows in the 80% group spent more time lying and ruminating between d -21 and d -7 and tended to ruminate more between d -14 and d 0. Calcium levels tended to be higher for cows in the 80% group, no other observable differences were found in monitored blood parameters. Moreover, 3.5% fat corrected milk and energy corrected milk yields were higher in 80% group in the first month of lactation. No other observable differences were found in the yield and composition of colostrum and milk in the first 10 months of lactation. The growth and performance of calves in the first week of life was not affected by stocking density of the dams.Conclusion: We concluded that lower stocking density may increase lying and ruminating behavior of prepartum Holstein cows. However, this did not translate into improved productivity and metabolism.

Functional significance of cholesterol metabolism in cancer: from threat to treatment

Xiao Mingming,Xu Jin,Wang Wei,Zhang Bo,Liu Jiang,Li Jialin,Xu Hang,Zhao Yingjun,Yu Xianjun,Shi Si 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Cholesterol is an essential structural component of membranes that contributes to membrane integrity and fluidity. Cholesterol homeostasis plays a critical role in the maintenance of cellular activities. Recently, increasing evidence has indicated that cholesterol is a major determinant by modulating cell signaling events governing the hallmarks of cancer. Numerous studies have shown the functional significance of cholesterol metabolism in tumorigenesis, cancer progression and metastasis through its regulatory effects on the immune response, ferroptosis, autophagy, cell stemness, and the DNA damage response. Here, we summarize recent literature describing cholesterol metabolism in cancer cells, including the cholesterol metabolism pathways and the mutual regulatory mechanisms involved in cancer progression and cholesterol metabolism. We also discuss various drugs targeting cholesterol metabolism to suggest new strategies for cancer treatment.

Performance of Homologous and Heterologous Prime-Boost Immunization Regimens of Recombinant Adenovirus and Modified Vaccinia Virus Ankara Expressing an Ag85B-TB10.4 Fusion Protein against Mycobacterium tuberculosis

( Yiming Kou ),( Mingming Wan ),( Wei Shi ),( Jie Liu ),( Zhilei Zhao ),( Yongqing Xu ),( Wei Wei ),( Bo Sun ),( Feng Gao ),( Linjun Cai ),( Chunlai Jiang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.6

Tuberculosis (TB) remains a serious health issue around the word. Adenovirus (Ad)-based vaccine and modified vaccinia virus Ankara (MVA)-based vaccine have emerged as two of the most promising immunization candidates over the past few years. However, the performance of the homologous and heterologous prime-boost immunization regimens of these two viral vector-based vaccines remains unclear. In the present study, we constructed recombinant Ad and MVA expressing an Ag85B-TB10.4 fusion protein (AdH4 and MVAH4) and evaluated the impact of their different immunization regimens on the humoral and cellular immune responses. We found that the viral vector-based vaccines could generate significantly higher levels of antigen-specific antibodies, IFN-γ-producing splenocytes, CD69⁺CD8⁺ T cells, and IFN-γ secretion when compared with bacillus Calmette-Guerin (BCG) in a mouse model. AdH4-containing immunization regimens (AdH4-AdH4, AdH4-MVAH4, and MVAH4-AdH4) induced significantly stronger antibody responses, much more IFN-γ-producing splenocytes and CD69⁺CD8⁺ T cells, and higher levels of IFN-γ secretion when compared with the MVAH4-MVAH4 immunization regimen. The number of IFN-γ-producing splenocytes sensitive to CD8⁺ T-cell restricted peptides of Ag85B (9-1p and 9-2p) and Th1-related cytokines (IFN-γ and TNF-α) in the AdH4-MVAH4 heterologous prime-boost regimen immunization group was significantly higher than that in the other viral vector-based vaccine- and BCG-immunized groups, respectively. These results indicate that an immunization regimen involving AdH4 may have a higher capacity to induce humoral and cellular immune responses against TB in mice than that by regimens containing BCG or MVAH4 alone, and the AdH4-MVAH4 prime-boost regimen may generate an ideal protective effect.

EMOJIS AND BRAND SELF-REPRESENTATION: A TEXT ANALYTICS APPROACH

Xiaowei Wang,,Mingming Cheng,Shanshi Li,Ruochen Jiang 글로벌지식마케팅경영학회 2020 Global Marketing Conference Vol.2020 No.11

Simpler Efficient Group Signature Scheme with Verifier-Local Revocation from Lattices

( Yanhua Zhang ),( Yupu Hu ),( Wen Gao ),( Mingming Jiang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.1

Verifier-local revocation (VLR) seems to be the most flexible revocation approaches for any group signature scheme, because it just only requires the verifiers to possess some up-to-date revocation information, but not the signers. Langlois et al. (PKC 2014) proposed the first VLR group signature based on lattice assumptions in the random oracle model. Their scheme has at least O(n²)·logN bit group public key and O (n) ·log N bit signature, respectively. Here, n is the security parameter and N is the maximum number of group members. In this paper, we present a simpler lattice-based VLR group signature, which is more efficient by a(log)ONfactor in both the group public key and the signature size. The security of our VLR group signature can be reduced to the hardness of learning with errors (LWE) and small integer solution (SIS) in the random oracle model.

Efficient Certificateless Signature Scheme on NTRU Lattice

( Jia Xie ),( Yupu Hu ),( Juntao Gao ),( Wen Gao ),( Mingming Jiang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.10

Because of the advantages of certificateless and no escrow feature over the regular signature and identity-based signature, certificateless signature has been widely applied in e-business, e-government and software security since it was proposed in 2003. Although a number of certificateless signature schemes have been proposed, there is only one lattice-based certificateless signature scheme which is still secure in the quantum era. But its efficiency is not very satisfactory. In this paper, the first certificateless signature scheme on NTRU lattice is proposed, which is proven to be secure in random oracle model. Moreover, the efficiency of the new scheme is higher than that of the only one lattice-based certificateless signature.

FLOURY ENDOSPERM12 Encoding Alanine Aminotransferase 1 Regulates Carbon and Nitrogen Metabolism in Rice

Mingsheng Zhong,Xi Liu,Feng Liu,Yulong Ren,Yunlong Wang,Jianping Zhu,Xuan Teng,Erchao Duan,Fan Wang,Huan Zhang,Mingming Wu,Yuanyuan Hao,Xiaopin Zhu,Ruonan Jing,Xiuping Guo,Ling Jiang,Yihua Wang,Jianmi 한국식물학회 2019 Journal of Plant Biology Vol.62 No.1

Starch is a major storage substance in cerealgrains, and starch biosynthesis is a complex process. In orderto elucidate regulation of the starch biosynthesis pathway, wescreened a series of rice (Oryza sativa L.) endospermmutants. In this study, we identified a floury white-coreendosperm mutant named floury endosperm12 (flo12). Theflo12 mutant exhibited loosely packed starch granules and alower thousand kernel weight compared to wild type. Semithinsections revealed that compound starch grains (SG) inflo12 interior endosperm cells were developed abnormally. Furthermore, amylose content was decreased, while totalprotein content was significantly increased in flo12 grains. Map-based cloning showed that FLO12 encodes rice alanineaminotransferase 1 (OsAlaAT1). OsAlaAT1 is highly expressedin developing endosperm. Subcellular localization showedthat OsAlaAT1 is localized in the cytosol. Moreover, theexpression of most starch synthesis-related genes wasdecreased, while most of the storage protein coding geneshad elevated expression levels in the flo12 mutant. Inaddition, overexpression of the OsAlaAT1 gene increasedgrain weight. In brief, we demonstrated that OsAlaAT1regulates carbon and nitrogen metabolism, which provides anew insight for the improvement of rice quality and yield.