Simple strategies for haplotype analysis of the X chromosome with application to age-related macular degeneration

Jiang, Renfang,Dong, Jianping,Joo, Jungnam,Geller, Nancy L,Zheng, Gang Macmillan Publishers Limited 2011 European journal of human genetics Vol.19 No.7

For haplotype analysis of the X chromosome, haplotype-sharing (HS) statistics with sliding windows are defined for males and females separately, which are then combined to a single HS test for the X chromosome. When independent replication samples are not available, the training-testing sets approach is used to validate this procedure and a permutation method is used to obtain its P-value. We applied this method to the X chromosome (with 1804 SNPs) for age-related macular degeneration (AMD). We found a window of five SNPs over a 272 kb region associated with AMD after Bonferroni correction. An examination of the odds ratio and the population attributable risks revealed a disease-preventive haplotype, ATGAC, on these five SNPs. For elderly females without this haplotype, the likelihood of AMD is increased by a factor of 4.75 with a 95% confidence interval (1.43, 15.82). The frequency of ATGAC in HapMap CEU is 0.276. These five SNPs are covered by the gene DIAPH2, which is known to cause premature ovarian failure (POF) in females. Our results indicated that DIAPH2 may be a polygenic pleiotropy for POF and AMD.

Silencing of Long Non-Coding RNA MALAT1 Promotes Apoptosis of Glioma Cells

Jianping Xiang,Shifeng Guo,Shuling Jiang,Yuelong Xu,Jiwei Li,Li Li,Jinyu Xiang 대한의학회 2016 Journal of Korean medical science Vol.31 No.5

The metastasis-associated lung adenocarcinoma transcription 1 (MALAT1) is a highly conserved long non-coding RNA (lncRNA) gene. However, little is known about the pathological role of lncRNA MALAT1 in glioma. In the present study, we explored the expression level of lncRNA MALAT1 in primary glioma tissues as well as in U87 and U251 glioma cell lines. Using qRT-PCR, we found that the expression of lncRNA MALAT1 was significantly increased in glioma tissues compared with that of paracancerous tissues. Meanwhile, the expression of MALAT1 was highly expressed in U98 and U251 cells. In order to explore the function of MALAT1, the expression of MALAT1 was greatly reduced in U87 and U251 cells transfected with siRNA specifically targeting MALAT1. Consequently, cell viability of U87 and U251 cells were drastically decreased after the knockdown of MALAT1. Concomitantly, the apoptosis rate of the two cell lines was dramatically increased. Furthermore, the expression levels of some tumor markers were reduced after the knockdown of MALAT1, such as CCND1 and MYC. In summary, the current study indicated a promoting role of MALAT1 in the development of glioma cell.

Effect of Multi-Level Microstructure on Local and Bulk Mechanical Properties in Micro-Injection Molded PC/PET Blend

Jianping Ren,Jing Jiang,Zihui Li,Jianhua Hou,Qian Li 한국고분자학회 2020 Macromolecular Research Vol.28 No.10

This study introduces a method to investigate the relationship between the multi-level microstructures and mechanical properties of polymer blends prepared by micro-injection molding (μIM). Special morphological features were systematically researched. Polycarbonate (PC), poly(ethylene terephthalate) (PET), and PC/PET microparts all exhibit typical “skin-core” morphologies. The thickness of the core layer is much greater than that of the skin layer, and the thickness of the skin layer gradually decreases along the flow direction. Photoacoustic Fourier transform infrared spectroscopy records reveal that the PC molecular chain has the biggest orientation degree, followed by PC/PET and PET chains under the same μIM processing conditions. Moreover, the molecular chains orientation in the skin layer is more than 50% that in the core layer. Nanoindentation tests are conducted to study local mechanical properties. The higher modulus in the shear layer is affected to a greater extent by high shear action in comparison with the frozen and core layers. Uniaxial tensile testing demonstrates that the tensile strength of PC/PET micropart is 15.5% higher than that of the PET micropart, while the toughness is 16% higher than that of the PC microparts. In-situ, high- speed tensile imaging, combined with scanning electron microscopy micrographs of the fracture section, are used to study the fracture behaviors of the microparts. The results gathered in this paper may provide a theoretical basis and data to support the feasibility and efficiency of micro-injection molded polymer blends.

SEMISUPERVISED CLASSIFICATION FOR FAULT DIAGNOSIS IN NUCLEAR POWER PLANTS

JIANPING MA,Jin Jiang 한국원자력학회 2015 Nuclear Engineering and Technology Vol.47 No.2

Pattern classifications have become important tools for fault diagnosis in nuclear powerplants (NPP). However, it is often difficult to obtain training data under fault conditionsto train a supervised classification model. By contrast, normal plant operating data canbe easily made available through increased deployment of supervisory, control, and dataacquisition systems. Such data can also be used to train classification models to improvethe performance of fault diagnosis scheme. In this paper, a fault diagnosis scheme based on semisupervised classification (SSC)scheme is developed. In this scheme, new measurements collected from the plant areintegrated with data observed under fault conditions to train the SSC models. The trainedmodels are subsequently applied to new measurements for fault diagnosis. In comparisonwith supervised classifiers, the proposed scheme requires significantly fewer data collectedunder fault conditions to train the classifier. The developed scheme has been validated using different fault scenarios on a desktopNPP simulator as well as on a physical NPP simulator using a graph-based SSC algorithm. All the considered faults have been successfully diagnosed. The results have demonstrated that SSC is a promising tool for fault diagnosis in NPPs

INTERDECADAL VARIATIONS OF EAST ASIAN SUMMER MONSOON NORTHWARD PROPAGATION AND INFLUENCES ON SUMMER PRECIPITATION OVER EAST CHINA

Zhihong Jiang,Song Yang,Jinhai He,Jianping Li 한국기상학회 2006 한국기상학회 학술대회 논문집 Vol.2006 No.-

Transcriptome-based identification of the optimal reference genes as internal controls for quantitative RT-PCR in razor clam (Sinonovacula constricta)

Xuelin Zhao,Jianping Fu,Liting Jiang,Weiwei Zhang,Yina Shao,Chunhua Jin,Jinbo Xiong,Chenghua Li 한국유전학회 2018 Genes & Genomics Vol.40 No.6

Quantitative real-time PCR (qRT-PCR) is a standard method to measure gene expression in function exploring. Accurate and reproducible data of qRT-PCR requires appropriate reference genes, which are stably expressed under different experimental conditions. However, no housekeeping genes were validated as internal controls for qRT-PCR in Sinonovacula constricta. In this study, we classified the transcriptome data of two tissues for Vibrio infection and Cd2+ stress into ten clusters based on the gene expression patterns. Among them, cluster 5 had the most stable gene expression patterns regardless of tissues and treatments as the database for candidate reference genes. A total of 55 orthologs of classical housekeeping genes in the clam transcriptome were annotated. Combined the expression profiles and housekeeping genes in S. constricta, we chose eight candidate reference genes and validated their expression in Vibrio-infected samples and different tissues by qRT-PCR. Their expression stability was analyzed by three different algorithms geNorm, NormFinder and BestKeeper. Although the rank of the eight candidate reference genes is different in different treatments using different software, RS9 could be the best reference genes for normalization of qRT-PCR expression data in S. constricta under various treatments considering the above analysis. Meanwhile, the ranking of genes based on the CV values of transcriptomic data was similar to the validation results. This study provides for the first time a list of suitable reference genes for S. constricta and a valuable resource for further studies of clam immune defense systems.

Elucidating the Role of Anthropogenic Aerosols in Arctic Sea Ice Variations

Wang, Yuan,Jiang, Jonathan H.,Su, Hui,Choi, Yong-Sang,Huang, Lei,Guo, Jianping,Yung, Yuk L. American Meteorological Society 2018 Journal of climate Vol.31 No.1

A Study on Multivariable Optimization in Precision Manufacturing Using MOPSONNS

Zhaopeng He,Tielin Shi,Jianping Xuan,Su Jiang,Yinfeng Wang 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.11

7075 aluminum alloy has been widely applied in the field of aerospace and marine sheet metal because of its protruding mechanical and corrosion resistance. In this paper, the problem of selecting optimal process parameters to optimize multiple processing variables had been studied in precision manufacturing. Multi-objective particle swarm optimized neural networks system was put forward to determine the optimal cutting conditions with multi-objective particle swarm algorithm and multiple neural networks as prediction models of machining variables. Precision parts manufacturing of 7075 aluminum alloy would go through two operations of material removal and surface forming. Firstly, optimal cutting conditions were determined to minimize tool wear while maximizing metal removal rate in material removal stage. Secondly, it was significant and meaningful to select optimal cutting conditions corresponding to the best surface quality and minimum root mean square of tool vibration in surface forming stage. Orthogonal experiments had been carried out to observe the relationship between machining-related variables and cutting parameters in detail. Multiple neural networks were trained to establish predictive models of cutting process from orthogonal experimental and statistical data. Maximum deviation theory sorted the Pareto solutions searched by optimization process of neural networks driven by multi-objective particle swarm algorithm. The top ranking Pareto solutions had been determined as the optimal cutting parameters combination for material removal and surface forming stages, respectively. Finally, the proposed optimization system can also be used to optimize the processing of other difficult-to-machine materials.

Efficient Bioreduction of Ethyl 4-chloro-3-oxobutanoate to (S)-chloro-3-hydrobutanoate by Whole Cells of Candida magnoliae in Water/n-Butyl Acetate Two-phase System

Zhinan Xu,Limei Fang,Jianping Lin,Xiaoxia Jiang 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.1

The asymmetric biosynthesis of ethyl (S)-4-chloro-3-hydrobutanoate from ethyl 4-chloro-3-oxobutanoate was investigated by using whole cells of Candida magnoliae JX120-3 without the addition of glucose dehydrogenase or NADP+/NADPH. In a one-phase system, the bioconversion yield was seriously affected on the addition of 12.1 g/L ethyl 4-chloro-3-oxobutanoate. In order to reduce this substrate inhibition, a water/n-butyl acetate two-phase system was developed, and the bioreduction conditions optimized with regard to the yield and product enantiometric excess value. The optimal conditions were as following: water to n-butyl acetate volume ratio of 1:1, 4.0 g DCW/L active cells, 50 g/L glucose and 35°C. By adopting a dropwise substrate feeding strategy, high concentration of ethyl 4-chloro-3-oxobutanoate (60 g/L) could be asymmetrically reduced to ethyl (S)-4-chloro-3-hydrobutanoate with high yield (93.8%) and high enantiometric excess value (92.7%).

Endotoxemia-mediated inflammation potentiates aminoglycoside-induced ototoxicity

Koo, Ja-Won,Quintanilla-Dieck, Lourdes,Jiang, Meiyan,Liu, Jianping,Urdang, Zachary D.,Allensworth, Jordan J.,Cross, Campbell P.,Li, Hongzhe,Steyger, Peter S. American Association for the Advancement of Scienc 2015 Science translational medicine Vol.7 No.298

<P>The ototoxic aminoglycoside antibiotics are essential to treat severe bacterial infections, particularly in neonatal intensive care units. Using a bacterial lipopolysaccharide (LPS) experimental model of sepsis, we tested whether LPS-mediated inflammation potentiates cochlear uptake of aminoglycosides and permanent hearing loss in mice. Using confocal microscopy and enzyme-linked immunosorbent assays, we found that low-dose LPS (endotoxemia) greatly increased cochlear concentrations of aminoglycosides and resulted in vasodilation of cochlear capillaries without inducing paracellular flux across the blood-labyrinth barrier (BLB) or elevating serum concentrations of the drug. Additionally, endotoxemia increased expression of both serum and cochlear inflammatory markers. These LPS-induced changes, classically mediated by Toll-like receptor 4 (TLR4), were attenuated in TLR4-hyporesponsive mice. Multiday dosing with aminoglycosides during chronic endotoxemia induced greater hearing threshold shifts and sensory cell loss compared to mice without endotoxemia. Thus, endotoxemia-mediated inflammation enhanced aminoglycoside trafficking across the BLB and potentiated aminoglycoside-induced ototoxicity. These data indicate that patients with severe infections are at greater risk of aminoglycoside-induced hearing loss than previously recognized.</P>