Effects of transcription factor SOX11 on the biological behavior of neuroblastoma cell and potential regulatory mechanism

Jing-Ru Huang,Yong Li,Peng Chen,Ji-Xiu Wei,Xia Yang,Qiong-Qian Xu,Jia-Bo Chen 대한외과학회 2024 Annals of Surgical Treatment and Research(ASRT) Vol.106 No.5

Purpose: This study aimed to analyze the expression and prognosis of SRY-box transcription factor 11 (SOX11) in neuroblastoma (NB), as well as the biological function and potential regulatory mechanism of SOX11 in NB. Methods: Public RNA sequencing was used to detect the expression level of SOX11. The Kaplan-Meier curve and hazard ratios (HR) were used to determine the prognostic value of SOX11 in NB. Functional analyses were performed using CCK8, wound healing assay, and transwell invasion assay. Finally, the potential target genes of SOX11 were predicted by Harmonizonme (Maayan Laboratory) and Cistrome Data Browser (Cistrome Project) database to explore the potential molecular mechanism of SOX11 in NB. Results: Compared with normal adrenal tissue, the expression of SOX11 in NB tissue was significantly upregulated. The Kaplan-Meier curve showed that high expression of SOX11 was associated with poor prognosis in children with NB (HR, 1.719; P = 0.049). SOX11 knockdown suppressed the migration capacity of SK-N-SH cells but did not affect proliferation and invasion capacity. Enhancer of zeste homolog 2 (EZH2) may be a potential downstream target gene for the transcription factor SOX11 to play a role in NB. Conclusion: The transcription factor SOX11 was significantly upregulated in NB. SOX11 knockdown suppressed the migration capacity of NB cell SK-N-SH. SOX11 may promote the progression of NB by targeting EZH2.

Association of polymorphisms in bone morphogenetic protein receptor-1B gene exon-9 with litter size in Dorset, Mongolian, and Small Tail Han ewes

Jianlei Jia,Qian Chen,Lin-sheng Gui,Jipeng Jin,Yongyuan Li,Qiaohong Ru,Shengzhen Hou 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.7

Objective: The present study was to investigate the association of polymorphisms in exon-9 of the bone morphogenetic protein receptor-1B (BMPR-1B) gene (C864T) with litter size in 240 Dorset, 232 Mongolian, and 124 Small Tail Han ewes. Methods: Blood samples were collected from 596 ewes and genomic DNA was extracted using the phenol: chloroform extraction method. The 304-bp amplified polymerase chain reaction product was analyzed for polymorphism by single-strand conformation polymorphism method. The genotypic frequency and allele frequency of BMPR-1B gene exon-9 were computed after sequence alignment. The χ2 independence test was used to analyze the association of genotypic frequency and litter size traits with in each ewe breed, where the phenotype was directly treated as category. Results: The results indicated two different banding patterns AA and AB for this fragment, with the most frequent genotype and allele of AA and A. Calculated Chi-square test for BMPR-1B gene exon-9 was found to be more than that of p value at the 5% level of significance, indicating that the population under study was in Hardy-Weinberg equilibrium for all ewes. The χ2 independence test analyses indicated litter size differences between genotypes was not the same for each breed. The 304-bp nucleotide sequence was subjected to BLAST analysis, and the C864T mutation significantly affected litter size in singletons, twins and multiples. The heterozygosity in exon-9 of BMPR-1B gene could increase litter size for all the studied ewes. Conclusion: Consequently, it appears that the polymorphism BMPR-1B gene exon-9 detected in this study may have potential use in marker assisted selection for litter size in Dorset, Mongolian, and Small Tail Han ewes.

Salt template-assisted construction of three-dimensional interconnected network in thermally conductive EP/PVDF/NiCo@GNP composites

Ning Jia,Bin Yang,Xiaohong Wang,Shishuang Cui,Peng Chen,Ru Xia,Zhengzhi Zheng,Jiasheng Qian,Yuchao Ke,Yang Pan 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-

In this study, graphene nanoplatelets (GNP) and nickel–cobalt (NiCo) alloy were adopted as reinforcingmaterials to prepare epoxy (EP) resin-based composites. NiCo particles were able to achieve uniformanchoring on GNPs’ surface under the electrostatic force due to the successful introduction of oxygendefects on GNPs. With the synergistic effect of adhesive PVDF and salt particles, the system with threedimensionally(3D) interconnected network was successfully constructed. Benefit from the formation ofmechanically stable and efficient heat conduction paths, the thermal conductivity (TC) of the EP/PVDF/NiCo@GNP composite reached 1.077 Wm1K1 at a filler volume fraction of 6.82 vol%, while the counterpart’sstoragemodulus (E’) was up to 3.22 GPa. In addition, a scaling formula was quantitatively proposed tocorrelate TC with E’, and the perfect agreement between the theoretical prediction and experimental dataclarified the underlying mechanism of the relationship between phonons transport and stress transferwithin a systemwith 3D ordered structures. The establishment of relationship between AHR (average heatingrate) and TC made it possible to further predict TC of composites based upon the infrared thermal imaging(ITI) data. The as-prepared GNP composites show good application prospects as thermal managementmaterials which need to experience thermally mechanical deformation.

Study on Crystallization Kinetics of Dynamically-Vulcanized PP/EPDM Blends

Bin Yang,Yan-Li Deng,Xia Ru,Ji-Bin Miao,Ming Cao,Jia-Sheng Qian,Li-Fen Su,Peng Chen,Jing-Wang Liu,La-Xia Wu,Tao Pang 한국고분자학회 2016 폴리머 Vol.40 No.4

Two types of β nucleating agents (β-NAs), aryl dicarboxylic acid amide (TMB-5) and diphenyl phthalate diamine (NT-C), were adopted to modify the polypropylene (PP)/ethylene propylene diene monomer (EPDM) blends, which were prepared by dynamic-vulcanization technology. Wide angle X-ray diffraction (WAXD) and differential scanning calorimetry (DSC) were used to study the crystallization kinetics of PP. Our results showed that the addition of β-NAs can considerably increase the crystallization temperature, and significantly decrease the spherulite size of β-PP (L300). The Jeziorny analysis showed there were ~82% and ~89% of relative crystallinity generated from the primary crystallization in the composites containing TMB-5 and NT-C, respectively. The crystallization half time (t0.5) showed that NT-C improved the overall crystallization rate more effectively than TMB-5. In addition, the peaks of the relative crystallization rate curves were shifted towards higher temperature by 14 and 9℃ with the addition of TMB-5 and NT-C, respectively.

Evaluation of Non-isothermal Melt Crystallization Kinetics of the Poly(lactic acid)/Graphene Composites under Variant Cooling Circumstances via Solidification Analysis Approach

Bin Yang,Peng Zhang,Shu-Chun Zhao,Ru Xia,Li-Fen Su,Ji-Bin Miao,Peng Chen,Jia-Sheng Qian,You Shi 한국고분자학회 2019 폴리머 Vol.43 No.5

The poly(lactic acid) (PLA)/graphene (GN) composites were prepared by solution blending. An in situ measurement technique was adopted to examine the non-isothermal crystallization of PLA/GN composites, which was different from the typical circumstances at constant cooling rate in DSC. The rheological properties of the samples were investigated, and the Carreau-A model was applied to non-linearly fit the rheological data. The non-isothermal crystallization kinetics and solidification behaviors at variant cooling rates were investigated using a modified version of Avrami equation by Jeziorny coupled with three-parameter model (TPM) and four-parameter model (FPM). Our findings showed that the viscosity of the PLA/GN composites dropped with increasing shear rate or GN content. Crystallization kinetic analysis suggested that GN served as an effective nucleating agent for PLA under non-isothermal melt crystallization conditions with variant cooling rate.

Nonlinear time history analysis of a pre-stressed concrete containment vessel model under Japan’s March 11 earthquake

Zuo-zhou Zhao,An Duan,Ju Che,Jia-ru Qian,Wei-liang Jin 사단법인 한국계산역학회 2014 Computers and Concrete, An International Journal Vol.13 No.1

To evaluate the behavior of the advanced unbonded pre-stressed concrete containment vessel (UPCCV) for one typical China nuclear power plant under Japan’s March 11 earthquake, five nonlinear time history analysis and a nonlinear static analysis of a 1:10 scale UPCCV structure have been carried out with MSC.MARC finite element program. Comparisons between the analytical and experimental results demonstrated that the developed finite element model can predict the earthquake behavior of the UPCCV with fair accuracy. The responses of the 1:10 scale UPCCV subjected to the 11 March 2011 Japan earthquakes recorded at the MYG003 station with the peak ground acceleration (PGA) of 781 gal and at the MYG013 station with the PGA of 982 gal were predicted by the dynamic analysis. Finally, a static analysis was performed to seek the ultimate load carrying capacity for the 1:10 scale UPCCV.

A Novel Kinetic Analysis of Crystallization of Polypropylene (PP) in Dynamically-vulcanized PP/Ethylene-propylene-diene Rubber (EPDM) Blends Using an In-situ Measurement Technique

Lei Hu,Bin Yang,Yan-Li Deng,Fei-Xue Lu,Ru Xia,Zheng-Zhi Zheng,Ji-Bin Miao,Jia-Sheng Qian,Chuan-Ru Zhang,Peng Chen,Yu-Chuan Zhang 한국고분자학회 2017 폴리머 Vol.41 No.4

The effects of cooling medium temperatures and plastic/rubber ratios on solidification and crystallization kinetics of dynamically-vulcanized polypropylene/ethylene-propylene-diene rubber (PP/EPDM) blends were investigated with the aid of an in-situ measurement technique. The cooling medium temperature heavily influenced the solidification kinetics primarily due to a combination of latent heat liberated from the molten polymer and the heat transferred away via the metallic wall during the cooling period. Interestingly, the parameter C in three-parameter model was not only affected by the material properties, but also by the cooling condition, different from the previous literature. The crystallization kinetics analysis indicated that the effect of EPDM in the blends consisted of both nucleation-promoting effect (low EPDM loading) and steric effect (higher EPDM loading). The present kinetic analysis may be helpful to further studies on improving the product performances for industrial applications.

In situ Copolymerized Toughened Polymethyl Methacrylate (PMMA) with Highly Transparency for Support Film of Polarizers

Yingying Wang,Bin Yang,Liangyong He,Yuqing Yang,Nuo Zhang,Yang Wang,Zhiqiang Shi,Yuchao Ke,Lifen Su,Jia-Sheng Qian,RU XIA,Tao Jiang 한국고분자학회 2022 폴리머 Vol.46 No.5

In this study, a series of poly(methyl methacrylate) (PMMA) copolymer films were prepared via solutionpolymerization of methyl methacrylate (MMA) with butyl acrylate (BA) and lauryl methacrylate (LMA) as monomers. Mechanical properties, hydrophobic properties, and optical properties of the films were intensively investigated. The rheological results showed that the fluidity of the copolymer was considerably enhanced. When the monomer ratio ofMMA:BA:LMA was 100:30:10, the copolymer film S4 showed the best overall performance with perfect optical transparency maintained. The results of the dynamic mechanical and thermal analysis suggested that the glass transition temperature (Tg) moved towards lower temperature, with enhanced ductility of the PMMA films. A large number of yieldfolds and crazes appeared on the cross-sectional surface of copolymer films through morphological observations, displaying the obvious characteristics of toughness fracture and obeying the energy dissipation mechanism of cracks shearband. The present study provided a facile way of preparing PMMA films with high toughness and light transmittanceby appropriate selection of the monomers, which will be of practical significance for further studies on the replacementof triacetyl cellulose as a support film of polarizers.

Non-isothermal Crystallization Behavior, Rheological and Thermal Conductive Properties of Recycled Polyethylene Terephthalate/Polyethylene Blends

Bin Yang,Dan Wang,Qin-Ting Chen,Jin Chen,Kang Chen,Ji-Bin Miao,Jia-Sheng Qian,Ru Xia,You Shi 한국고분자학회 2020 폴리머 Vol.44 No.3

In this study, we prepared series of recycled polyethylene terephthalate (RPET)/polyethylene (PE) blends using melt extrusion. The effect of RPET content on crystallization behavior and thermal conductive properties of the resultant blends were investigated using differential scanning calorimetry (DSC), dynamic mechanical thermal analysis (DMTA), etc. RPET was found to exert nucleating effect on the melt crystallization of PE. The Agari model presented fairly reasonable prediction of thermal conductivity as a function of RPET loading. The melt cooling process was predicted with an enthalpy transformation method (ETM), which is a well-established mean of evaluating the instantaneous heat conduction of crystalline polymers/composites, and the obtained curves were consistent with our experimental results. Besides, a four-parameter model (FPM) was adopted coupled with an in-situ temperature measurement in order to further disclose the solidification and crystallization kinetics of PE in the presence of RPET in the blends.